Id |
Subject |
Object |
Predicate |
Lexical cue |
T8821 |
0-7 |
NN |
denotes |
Protein |
T8822 |
8-16 |
NNS |
denotes |
extracts |
T8823 |
22-30 |
VBN |
denotes |
isolated |
T8824 |
17-21 |
VBD |
denotes |
were |
T8825 |
31-35 |
IN |
denotes |
from |
T8826 |
36-47 |
VBN |
denotes |
homogenized |
T8827 |
48-53 |
NN |
denotes |
liver |
T8828 |
54-55 |
-LRB- |
denotes |
( |
T8829 |
55-62 |
RB |
denotes |
freshly |
T8830 |
63-72 |
VBN |
denotes |
harvested |
T8831 |
73-76 |
CC |
denotes |
and |
T8832 |
77-82 |
NN |
denotes |
flash |
T8833 |
83-89 |
VBN |
denotes |
frozen |
T8834 |
89-90 |
-RRB- |
denotes |
) |
T8835 |
91-93 |
IN |
denotes |
of |
T8836 |
94-102 |
NN |
denotes |
wildtype |
T8837 |
126-136 |
JJ |
denotes |
transgenic |
T8838 |
102-104 |
, |
denotes |
, |
T8839 |
104-108 |
NN |
denotes |
Line |
T8840 |
109-113 |
NN |
denotes |
525A |
T8841 |
113-115 |
, |
denotes |
, |
T8842 |
115-118 |
CC |
denotes |
and |
T8843 |
119-123 |
NN |
denotes |
Line |
T8844 |
124-125 |
NN |
denotes |
A |
T8845 |
137-141 |
NNS |
denotes |
mice |
T8846 |
141-142 |
. |
denotes |
. |
T8847 |
142-329 |
sentence |
denotes |
Protein (25 μg per sample) was analyzed by electrophoresis on a 10% SDS-PAGE Ready Gel (Bio-Rad, Hercules, CA) and transferred to PVDF membrane (Sequi-Blot; Bio-Rad) via electroblotting. |
T8848 |
143-150 |
NN |
denotes |
Protein |
T8849 |
174-182 |
VBN |
denotes |
analyzed |
T8850 |
151-152 |
-LRB- |
denotes |
( |
T8851 |
155-157 |
NN |
denotes |
μg |
T8852 |
152-154 |
CD |
denotes |
25 |
T8853 |
158-161 |
IN |
denotes |
per |
T8854 |
162-168 |
NN |
denotes |
sample |
T8855 |
168-169 |
-RRB- |
denotes |
) |
T8856 |
170-173 |
VBD |
denotes |
was |
T8857 |
183-185 |
IN |
denotes |
by |
T8858 |
186-201 |
NN |
denotes |
electrophoresis |
T8859 |
202-204 |
IN |
denotes |
on |
T8860 |
205-206 |
DT |
denotes |
a |
T8861 |
226-229 |
NN |
denotes |
Gel |
T8862 |
207-209 |
CD |
denotes |
10 |
T8863 |
209-210 |
NN |
denotes |
% |
T8864 |
211-214 |
NN |
denotes |
SDS |
T8865 |
215-219 |
NN |
denotes |
PAGE |
T8866 |
214-215 |
HYPH |
denotes |
- |
T8867 |
220-225 |
JJ |
denotes |
Ready |
T8868 |
230-231 |
-LRB- |
denotes |
( |
T8869 |
235-238 |
NNP |
denotes |
Rad |
T8870 |
231-234 |
NNP |
denotes |
Bio |
T8871 |
234-235 |
HYPH |
denotes |
- |
T8872 |
238-240 |
, |
denotes |
, |
T8873 |
240-248 |
NNP |
denotes |
Hercules |
T8874 |
248-250 |
, |
denotes |
, |
T8875 |
250-252 |
NNP |
denotes |
CA |
T8876 |
252-253 |
-RRB- |
denotes |
) |
T8877 |
254-257 |
CC |
denotes |
and |
T8878 |
258-269 |
VBN |
denotes |
transferred |
T8879 |
270-272 |
IN |
denotes |
to |
T8880 |
273-277 |
NN |
denotes |
PVDF |
T8881 |
278-286 |
NN |
denotes |
membrane |
T8882 |
287-288 |
-LRB- |
denotes |
( |
T8883 |
304-307 |
NNP |
denotes |
Rad |
T8884 |
288-293 |
NNP |
denotes |
Sequi |
T8885 |
294-298 |
NNP |
denotes |
Blot |
T8886 |
293-294 |
HYPH |
denotes |
- |
T8887 |
298-299 |
: |
denotes |
; |
T8888 |
300-303 |
NNP |
denotes |
Bio |
T8889 |
303-304 |
HYPH |
denotes |
- |
T8890 |
307-308 |
-RRB- |
denotes |
) |
T8891 |
309-312 |
IN |
denotes |
via |
T8892 |
313-328 |
NN |
denotes |
electroblotting |
T8893 |
328-329 |
. |
denotes |
. |
T8894 |
329-538 |
sentence |
denotes |
All blots were probed for E2 protein using polyclonal rabbit E2 antisera (1:5,000), which detects both mouse (~47 kD) and human (~54 kD) E2 subunits ([30]; gift from Dr. Susan Hutson, Wake Forest University). |
T8895 |
330-333 |
DT |
denotes |
All |
T8896 |
334-339 |
NNS |
denotes |
blots |
T8897 |
345-351 |
VBN |
denotes |
probed |
T8898 |
340-344 |
VBD |
denotes |
were |
T8899 |
352-355 |
IN |
denotes |
for |
T8900 |
356-358 |
NN |
denotes |
E2 |
T8901 |
359-366 |
NN |
denotes |
protein |
T8902 |
367-372 |
VBG |
denotes |
using |
T8903 |
373-383 |
JJ |
denotes |
polyclonal |
T8904 |
394-402 |
NNS |
denotes |
antisera |
T8905 |
384-390 |
NN |
denotes |
rabbit |
T8906 |
391-393 |
NN |
denotes |
E2 |
T8907 |
403-404 |
-LRB- |
denotes |
( |
T8908 |
406-411 |
CD |
denotes |
5,000 |
T8909 |
404-405 |
CD |
denotes |
1 |
T8910 |
405-406 |
SYM |
denotes |
: |
T8911 |
411-412 |
-RRB- |
denotes |
) |
T8912 |
412-414 |
, |
denotes |
, |
T8913 |
414-419 |
WDT |
denotes |
which |
T8914 |
420-427 |
VBZ |
denotes |
detects |
T8915 |
428-432 |
CC |
denotes |
both |
T8916 |
470-478 |
NNS |
denotes |
subunits |
T8917 |
433-438 |
NN |
denotes |
mouse |
T8918 |
439-440 |
-LRB- |
denotes |
( |
T8919 |
444-446 |
NN |
denotes |
kD |
T8920 |
440-441 |
SYM |
denotes |
~ |
T8921 |
441-443 |
CD |
denotes |
47 |
T8922 |
446-447 |
-RRB- |
denotes |
) |
T8923 |
448-451 |
CC |
denotes |
and |
T8924 |
452-457 |
JJ |
denotes |
human |
T8925 |
458-459 |
-LRB- |
denotes |
( |
T8926 |
463-465 |
NN |
denotes |
kD |
T8927 |
459-460 |
SYM |
denotes |
~ |
T8928 |
460-462 |
CD |
denotes |
54 |
T8929 |
465-466 |
-RRB- |
denotes |
) |
T8930 |
467-469 |
NN |
denotes |
E2 |
T8931 |
479-480 |
-LRB- |
denotes |
( |
T8932 |
486-490 |
NN |
denotes |
gift |
T8933 |
480-481 |
-LRB- |
denotes |
[ |
T8934 |
481-483 |
CD |
denotes |
30 |
T8935 |
483-484 |
-RRB- |
denotes |
] |
T8936 |
484-485 |
: |
denotes |
; |
T8937 |
491-495 |
IN |
denotes |
from |
T8938 |
496-499 |
NNP |
denotes |
Dr. |
T8939 |
506-512 |
NNP |
denotes |
Hutson |
T8940 |
500-505 |
NNP |
denotes |
Susan |
T8941 |
512-514 |
, |
denotes |
, |
T8942 |
514-518 |
NNP |
denotes |
Wake |
T8943 |
519-525 |
NNP |
denotes |
Forest |
T8944 |
526-536 |
NNP |
denotes |
University |
T8945 |
536-537 |
-RRB- |
denotes |
) |
T8946 |
537-538 |
. |
denotes |
. |
T8947 |
538-649 |
sentence |
denotes |
Blots were re-probed with a rabbit anti-c-myc tag antibody (1:10,000; abCam, Cambridge, MA; cat.# ab9106-100). |
T8948 |
539-544 |
NNS |
denotes |
Blots |
T8949 |
550-559 |
VBN |
denotes |
re-probed |
T8950 |
545-549 |
VBD |
denotes |
were |
T8951 |
560-564 |
IN |
denotes |
with |
T8952 |
565-566 |
DT |
denotes |
a |
T8953 |
589-597 |
NN |
denotes |
antibody |
T8954 |
567-573 |
NN |
denotes |
rabbit |
T8955 |
574-580 |
JJ |
denotes |
anti-c |
T8956 |
581-584 |
NN |
denotes |
myc |
T8957 |
580-581 |
HYPH |
denotes |
- |
T8958 |
585-588 |
NN |
denotes |
tag |
T8959 |
598-599 |
-LRB- |
denotes |
( |
T8960 |
637-643 |
NN |
denotes |
ab9106 |
T8961 |
599-600 |
CD |
denotes |
1 |
T8962 |
601-607 |
CD |
denotes |
10,000 |
T8963 |
600-601 |
SYM |
denotes |
: |
T8964 |
607-608 |
: |
denotes |
; |
T8965 |
609-614 |
NNP |
denotes |
abCam |
T8966 |
614-616 |
, |
denotes |
, |
T8967 |
616-625 |
NNP |
denotes |
Cambridge |
T8968 |
625-627 |
, |
denotes |
, |
T8969 |
627-629 |
NNP |
denotes |
MA |
T8970 |
629-630 |
: |
denotes |
; |
T8971 |
631-635 |
NN |
denotes |
cat. |
T8972 |
635-636 |
SYM |
denotes |
# |
T8973 |
643-644 |
HYPH |
denotes |
- |
T8974 |
644-647 |
CD |
denotes |
100 |
T8975 |
647-648 |
-RRB- |
denotes |
) |
T8976 |
648-649 |
. |
denotes |
. |
T8977 |
649-780 |
sentence |
denotes |
Blots were also re-probed with an antibody for β-actin (43 kD; 1:10,000; abCam; cat.# ab8227-50) to allow for loading comparisons. |
T8978 |
650-655 |
NNS |
denotes |
Blots |
T8979 |
666-675 |
VBN |
denotes |
re-probed |
T8980 |
656-660 |
VBD |
denotes |
were |
T8981 |
661-665 |
RB |
denotes |
also |
T8982 |
676-680 |
IN |
denotes |
with |
T8983 |
681-683 |
DT |
denotes |
an |
T8984 |
684-692 |
NN |
denotes |
antibody |
T8985 |
693-696 |
IN |
denotes |
for |
T8986 |
697-698 |
NN |
denotes |
β |
T8987 |
699-704 |
NN |
denotes |
actin |
T8988 |
698-699 |
HYPH |
denotes |
- |
T8989 |
705-706 |
-LRB- |
denotes |
( |
T8990 |
736-742 |
NN |
denotes |
ab8227 |
T8991 |
706-708 |
CD |
denotes |
43 |
T8992 |
709-711 |
NN |
denotes |
kD |
T8993 |
711-712 |
: |
denotes |
; |
T8994 |
713-714 |
CD |
denotes |
1 |
T8995 |
715-721 |
CD |
denotes |
10,000 |
T8996 |
714-715 |
SYM |
denotes |
: |
T8997 |
721-722 |
: |
denotes |
; |
T8998 |
723-728 |
NN |
denotes |
abCam |
T8999 |
728-729 |
: |
denotes |
; |
T9000 |
730-734 |
NN |
denotes |
cat. |
T9001 |
734-735 |
SYM |
denotes |
# |
T9002 |
742-743 |
HYPH |
denotes |
- |
T9003 |
743-745 |
CD |
denotes |
50 |
T9004 |
745-746 |
-RRB- |
denotes |
) |
T9005 |
747-749 |
TO |
denotes |
to |
T9006 |
750-755 |
VB |
denotes |
allow |
T9007 |
756-759 |
IN |
denotes |
for |
T9008 |
760-767 |
NN |
denotes |
loading |
T9009 |
768-779 |
NNS |
denotes |
comparisons |
T9010 |
779-780 |
. |
denotes |
. |
T9011 |
780-1034 |
sentence |
denotes |
A goat anti-rabbit secondary antibody conjugated to horseradish peroxidase (1:10,000; Novus, Littleton, CO; cat.# NB730-H) was used for detection using the Western Lightning chemiluminescence reagent (Perkin Elmer, Boston, MA) and exposed to X-ray film. |
T9012 |
781-782 |
DT |
denotes |
A |
T9013 |
810-818 |
NN |
denotes |
antibody |
T9014 |
783-787 |
NN |
denotes |
goat |
T9015 |
788-799 |
JJ |
denotes |
anti-rabbit |
T9016 |
800-809 |
JJ |
denotes |
secondary |
T9017 |
908-912 |
VBN |
denotes |
used |
T9018 |
819-829 |
VBN |
denotes |
conjugated |
T9019 |
830-832 |
IN |
denotes |
to |
T9020 |
833-844 |
NN |
denotes |
horseradish |
T9021 |
845-855 |
NN |
denotes |
peroxidase |
T9022 |
856-857 |
-LRB- |
denotes |
( |
T9023 |
901-902 |
NN |
denotes |
H |
T9024 |
857-858 |
CD |
denotes |
1 |
T9025 |
859-865 |
CD |
denotes |
10,000 |
T9026 |
858-859 |
SYM |
denotes |
: |
T9027 |
865-866 |
: |
denotes |
; |
T9028 |
867-872 |
NNP |
denotes |
Novus |
T9029 |
872-874 |
, |
denotes |
, |
T9030 |
874-883 |
NNP |
denotes |
Littleton |
T9031 |
883-885 |
, |
denotes |
, |
T9032 |
885-887 |
NNP |
denotes |
CO |
T9033 |
887-888 |
: |
denotes |
; |
T9034 |
889-893 |
NN |
denotes |
cat. |
T9035 |
893-894 |
SYM |
denotes |
# |
T9036 |
895-900 |
NN |
denotes |
NB730 |
T9037 |
900-901 |
HYPH |
denotes |
- |
T9038 |
902-903 |
-RRB- |
denotes |
) |
T9039 |
904-907 |
VBD |
denotes |
was |
T9040 |
913-916 |
IN |
denotes |
for |
T9041 |
917-926 |
NN |
denotes |
detection |
T9042 |
927-932 |
VBG |
denotes |
using |
T9043 |
933-936 |
DT |
denotes |
the |
T9044 |
973-980 |
NN |
denotes |
reagent |
T9045 |
937-944 |
NNP |
denotes |
Western |
T9046 |
945-954 |
NNP |
denotes |
Lightning |
T9047 |
955-972 |
NN |
denotes |
chemiluminescence |
T9048 |
981-982 |
-LRB- |
denotes |
( |
T9049 |
989-994 |
NNP |
denotes |
Elmer |
T9050 |
982-988 |
NNP |
denotes |
Perkin |
T9051 |
994-996 |
, |
denotes |
, |
T9052 |
996-1002 |
NNP |
denotes |
Boston |
T9053 |
1002-1004 |
, |
denotes |
, |
T9054 |
1004-1006 |
NNP |
denotes |
MA |
T9055 |
1006-1007 |
-RRB- |
denotes |
) |
T9056 |
1008-1011 |
CC |
denotes |
and |
T9057 |
1012-1019 |
VBN |
denotes |
exposed |
T9058 |
1020-1022 |
IN |
denotes |
to |
T9059 |
1023-1028 |
NN |
denotes |
X-ray |
T9060 |
1029-1033 |
NN |
denotes |
film |
T9061 |
1033-1034 |
. |
denotes |
. |
R2510 |
T8821 |
T8822 |
compound |
Protein,extracts |
R2511 |
T8822 |
T8823 |
nsubjpass |
extracts,isolated |
R2512 |
T8824 |
T8823 |
auxpass |
were,isolated |
R2513 |
T8825 |
T8823 |
prep |
from,isolated |
R2514 |
T8826 |
T8827 |
amod |
homogenized,liver |
R2515 |
T8827 |
T8825 |
pobj |
liver,from |
R2516 |
T8828 |
T8827 |
punct |
(,liver |
R2517 |
T8829 |
T8830 |
advmod |
freshly,harvested |
R2518 |
T8830 |
T8827 |
acl |
harvested,liver |
R2519 |
T8831 |
T8830 |
cc |
and,harvested |
R2520 |
T8832 |
T8833 |
dep |
flash,frozen |
R2521 |
T8833 |
T8830 |
conj |
frozen,harvested |
R2522 |
T8834 |
T8827 |
punct |
),liver |
R2523 |
T8835 |
T8827 |
prep |
of,liver |
R2524 |
T8836 |
T8837 |
npadvmod |
wildtype,transgenic |
R2525 |
T8837 |
T8845 |
amod |
transgenic,mice |
R2526 |
T8838 |
T8836 |
punct |
", ",wildtype |
R2527 |
T8839 |
T8840 |
compound |
Line,525A |
R2528 |
T8840 |
T8836 |
conj |
525A,wildtype |
R2529 |
T8841 |
T8840 |
punct |
", ",525A |
R2530 |
T8842 |
T8840 |
cc |
and,525A |
R2531 |
T8843 |
T8844 |
compound |
Line,A |
R2532 |
T8844 |
T8840 |
conj |
A,525A |
R2533 |
T8845 |
T8835 |
pobj |
mice,of |
R2534 |
T8846 |
T8823 |
punct |
.,isolated |
R2535 |
T8848 |
T8849 |
nsubjpass |
Protein,analyzed |
R2536 |
T8850 |
T8851 |
punct |
(,μg |
R2537 |
T8851 |
T8848 |
parataxis |
μg,Protein |
R2538 |
T8852 |
T8851 |
nummod |
25,μg |
R2539 |
T8853 |
T8851 |
prep |
per,μg |
R2540 |
T8854 |
T8853 |
pobj |
sample,per |
R2541 |
T8855 |
T8851 |
punct |
),μg |
R2542 |
T8856 |
T8849 |
auxpass |
was,analyzed |
R2543 |
T8857 |
T8849 |
prep |
by,analyzed |
R2544 |
T8858 |
T8857 |
pobj |
electrophoresis,by |
R2545 |
T8859 |
T8849 |
prep |
on,analyzed |
R2546 |
T8860 |
T8861 |
det |
a,Gel |
R2547 |
T8861 |
T8859 |
pobj |
Gel,on |
R2548 |
T8862 |
T8863 |
nummod |
10,% |
R2549 |
T8863 |
T8861 |
nmod |
%,Gel |
R2550 |
T8864 |
T8865 |
nmod |
SDS,PAGE |
R2551 |
T8865 |
T8861 |
nmod |
PAGE,Gel |
R2552 |
T8866 |
T8865 |
punct |
-,PAGE |
R2553 |
T8867 |
T8861 |
amod |
Ready,Gel |
R2554 |
T8868 |
T8869 |
punct |
(,Rad |
R2555 |
T8869 |
T8861 |
parataxis |
Rad,Gel |
R2556 |
T8870 |
T8869 |
compound |
Bio,Rad |
R2557 |
T8871 |
T8869 |
punct |
-,Rad |
R2558 |
T8872 |
T8869 |
punct |
", ",Rad |
R2559 |
T8873 |
T8869 |
npadvmod |
Hercules,Rad |
R2560 |
T8874 |
T8869 |
punct |
", ",Rad |
R2561 |
T8875 |
T8869 |
npadvmod |
CA,Rad |
R2562 |
T8876 |
T8869 |
punct |
),Rad |
R2563 |
T8877 |
T8849 |
cc |
and,analyzed |
R2564 |
T8878 |
T8849 |
conj |
transferred,analyzed |
R2565 |
T8879 |
T8878 |
prep |
to,transferred |
R2566 |
T8880 |
T8881 |
compound |
PVDF,membrane |
R2567 |
T8881 |
T8879 |
pobj |
membrane,to |
R2568 |
T8882 |
T8883 |
punct |
(,Rad |
R2569 |
T8883 |
T8881 |
parataxis |
Rad,membrane |
R2570 |
T8884 |
T8885 |
compound |
Sequi,Blot |
R2571 |
T8885 |
T8883 |
dep |
Blot,Rad |
R2572 |
T8886 |
T8885 |
punct |
-,Blot |
R2573 |
T8887 |
T8883 |
punct |
;,Rad |
R2574 |
T8888 |
T8883 |
compound |
Bio,Rad |
R2575 |
T8889 |
T8883 |
punct |
-,Rad |
R2576 |
T8890 |
T8883 |
punct |
),Rad |
R2577 |
T8891 |
T8878 |
prep |
via,transferred |
R2578 |
T8892 |
T8891 |
pobj |
electroblotting,via |
R2579 |
T8893 |
T8849 |
punct |
.,analyzed |
R2580 |
T8895 |
T8896 |
det |
All,blots |
R2581 |
T8896 |
T8897 |
nsubjpass |
blots,probed |
R2582 |
T8898 |
T8897 |
auxpass |
were,probed |
R2583 |
T8899 |
T8897 |
prep |
for,probed |
R2584 |
T8900 |
T8901 |
compound |
E2,protein |
R2585 |
T8901 |
T8899 |
pobj |
protein,for |
R2586 |
T8902 |
T8897 |
advcl |
using,probed |
R2587 |
T8903 |
T8904 |
amod |
polyclonal,antisera |
R2588 |
T8904 |
T8902 |
dobj |
antisera,using |
R2589 |
T8905 |
T8904 |
compound |
rabbit,antisera |
R2590 |
T8906 |
T8904 |
compound |
E2,antisera |
R2591 |
T8907 |
T8908 |
punct |
(,"5,000" |
R2592 |
T8908 |
T8904 |
parataxis |
"5,000",antisera |
R2593 |
T8909 |
T8908 |
nummod |
1,"5,000" |
R2594 |
T8910 |
T8908 |
punct |
:,"5,000" |
R2595 |
T8911 |
T8908 |
punct |
),"5,000" |
R2596 |
T8912 |
T8904 |
punct |
", ",antisera |
R2597 |
T8913 |
T8914 |
dep |
which,detects |
R2598 |
T8914 |
T8904 |
relcl |
detects,antisera |
R2599 |
T8915 |
T8916 |
preconj |
both,subunits |
R2600 |
T8916 |
T8914 |
dobj |
subunits,detects |
R2601 |
T8917 |
T8916 |
nmod |
mouse,subunits |
R2602 |
T8918 |
T8919 |
punct |
(,kD |
R2603 |
T8919 |
T8917 |
parataxis |
kD,mouse |
R2604 |
T8920 |
T8921 |
punct |
~,47 |
R2605 |
T8921 |
T8919 |
nummod |
47,kD |
R2606 |
T8922 |
T8919 |
punct |
),kD |
R2607 |
T8923 |
T8917 |
cc |
and,mouse |
R2608 |
T8924 |
T8917 |
conj |
human,mouse |
R2609 |
T8925 |
T8926 |
punct |
(,kD |
R2610 |
T8926 |
T8924 |
parataxis |
kD,human |
R2611 |
T8927 |
T8928 |
punct |
~,54 |
R2612 |
T8928 |
T8926 |
nummod |
54,kD |
R2613 |
T8929 |
T8926 |
punct |
),kD |
R2614 |
T8930 |
T8916 |
compound |
E2,subunits |
R2615 |
T8931 |
T8932 |
punct |
(,gift |
R2616 |
T8932 |
T8916 |
parataxis |
gift,subunits |
R2617 |
T8933 |
T8932 |
punct |
[,gift |
R2618 |
T8934 |
T8932 |
dep |
30,gift |
R2619 |
T8935 |
T8932 |
punct |
],gift |
R2620 |
T8936 |
T8932 |
punct |
;,gift |
R2621 |
T8937 |
T8932 |
prep |
from,gift |
R2622 |
T8938 |
T8939 |
compound |
Dr.,Hutson |
R2623 |
T8939 |
T8937 |
pobj |
Hutson,from |
R2624 |
T8940 |
T8939 |
compound |
Susan,Hutson |
R2625 |
T8941 |
T8939 |
punct |
", ",Hutson |
R2626 |
T8942 |
T8943 |
compound |
Wake,Forest |
R2627 |
T8943 |
T8944 |
compound |
Forest,University |
R2628 |
T8944 |
T8939 |
npadvmod |
University,Hutson |
R2629 |
T8945 |
T8932 |
punct |
),gift |
R2630 |
T8946 |
T8897 |
punct |
.,probed |
R2631 |
T8948 |
T8949 |
nsubjpass |
Blots,re-probed |
R2632 |
T8950 |
T8949 |
auxpass |
were,re-probed |
R2633 |
T8951 |
T8949 |
prep |
with,re-probed |
R2634 |
T8952 |
T8953 |
det |
a,antibody |
R2635 |
T8953 |
T8951 |
pobj |
antibody,with |
R2636 |
T8954 |
T8953 |
nmod |
rabbit,antibody |
R2637 |
T8955 |
T8956 |
amod |
anti-c,myc |
R2638 |
T8956 |
T8953 |
compound |
myc,antibody |
R2639 |
T8957 |
T8956 |
punct |
-,myc |
R2640 |
T8958 |
T8953 |
compound |
tag,antibody |
R2641 |
T8959 |
T8960 |
punct |
(,ab9106 |
R2642 |
T8960 |
T8953 |
parataxis |
ab9106,antibody |
R2643 |
T8961 |
T8962 |
nummod |
1,"10,000" |
R2644 |
T8962 |
T8960 |
dep |
"10,000",ab9106 |
R2645 |
T8963 |
T8962 |
punct |
:,"10,000" |
R2646 |
T8964 |
T8960 |
punct |
;,ab9106 |
R2647 |
T8965 |
T8960 |
dep |
abCam,ab9106 |
R2648 |
T8966 |
T8965 |
punct |
", ",abCam |
R2649 |
T8967 |
T8965 |
npadvmod |
Cambridge,abCam |
R2650 |
T8968 |
T8965 |
punct |
", ",abCam |
R2651 |
T8969 |
T8965 |
npadvmod |
MA,abCam |
R2652 |
T8970 |
T8960 |
punct |
;,ab9106 |
R2653 |
T8971 |
T8960 |
nmod |
cat.,ab9106 |
R2654 |
T8972 |
T8960 |
punct |
#,ab9106 |
R2655 |
T8973 |
T8960 |
punct |
-,ab9106 |
R2656 |
T8974 |
T8960 |
nummod |
100,ab9106 |
R2657 |
T8975 |
T8960 |
punct |
),ab9106 |
R2658 |
T8976 |
T8949 |
punct |
.,re-probed |
R2659 |
T8978 |
T8979 |
nsubjpass |
Blots,re-probed |
R2660 |
T8980 |
T8979 |
auxpass |
were,re-probed |
R2661 |
T8981 |
T8979 |
advmod |
also,re-probed |
R2662 |
T8982 |
T8979 |
prep |
with,re-probed |
R2663 |
T8983 |
T8984 |
det |
an,antibody |
R2664 |
T8984 |
T8982 |
pobj |
antibody,with |
R2665 |
T8985 |
T8984 |
prep |
for,antibody |
R2666 |
T8986 |
T8987 |
compound |
β,actin |
R2667 |
T8987 |
T8985 |
pobj |
actin,for |
R2668 |
T8988 |
T8987 |
punct |
-,actin |
R2669 |
T8989 |
T8990 |
punct |
(,ab8227 |
R2670 |
T8990 |
T8987 |
parataxis |
ab8227,actin |
R2671 |
T8991 |
T8992 |
nummod |
43,kD |
R2672 |
T8992 |
T8990 |
dep |
kD,ab8227 |
R2673 |
T8993 |
T8990 |
punct |
;,ab8227 |
R2674 |
T8994 |
T8995 |
nummod |
1,"10,000" |
R2675 |
T8995 |
T8990 |
dep |
"10,000",ab8227 |
R2676 |
T8996 |
T8995 |
punct |
:,"10,000" |
R2677 |
T8997 |
T8990 |
punct |
;,ab8227 |
R2678 |
T8998 |
T8990 |
dep |
abCam,ab8227 |
R2679 |
T8999 |
T8990 |
punct |
;,ab8227 |
R2680 |
T9000 |
T8990 |
nmod |
cat.,ab8227 |
R2681 |
T9001 |
T8990 |
punct |
#,ab8227 |
R2682 |
T9002 |
T8990 |
punct |
-,ab8227 |
R2683 |
T9003 |
T8990 |
nummod |
50,ab8227 |
R2684 |
T9004 |
T8990 |
punct |
),ab8227 |
R2685 |
T9005 |
T9006 |
aux |
to,allow |
R2686 |
T9006 |
T8979 |
advcl |
allow,re-probed |
R2687 |
T9007 |
T9006 |
prep |
for,allow |
R2688 |
T9008 |
T9009 |
compound |
loading,comparisons |
R2689 |
T9009 |
T9007 |
pobj |
comparisons,for |
R2690 |
T9010 |
T8979 |
punct |
.,re-probed |
R2691 |
T9012 |
T9013 |
det |
A,antibody |
R2692 |
T9013 |
T9017 |
nsubjpass |
antibody,used |
R2693 |
T9014 |
T9013 |
nmod |
goat,antibody |
R2694 |
T9015 |
T9013 |
amod |
anti-rabbit,antibody |
R2695 |
T9016 |
T9013 |
amod |
secondary,antibody |
R2696 |
T9018 |
T9013 |
acl |
conjugated,antibody |
R2697 |
T9019 |
T9018 |
prep |
to,conjugated |
R2698 |
T9020 |
T9021 |
compound |
horseradish,peroxidase |
R2699 |
T9021 |
T9019 |
pobj |
peroxidase,to |
R2700 |
T9022 |
T9023 |
punct |
(,H |
R2701 |
T9023 |
T9021 |
parataxis |
H,peroxidase |
R2702 |
T9024 |
T9025 |
nummod |
1,"10,000" |
R2703 |
T9025 |
T9023 |
dep |
"10,000",H |
R2704 |
T9026 |
T9025 |
punct |
:,"10,000" |
R2705 |
T9027 |
T9023 |
punct |
;,H |
R2706 |
T9028 |
T9023 |
dep |
Novus,H |
R2707 |
T9029 |
T9028 |
punct |
", ",Novus |
R2708 |
T9030 |
T9028 |
npadvmod |
Littleton,Novus |
R2709 |
T9031 |
T9028 |
punct |
", ",Novus |
R2710 |
T9032 |
T9028 |
npadvmod |
CO,Novus |
R2711 |
T9033 |
T9023 |
punct |
;,H |
R2712 |
T9034 |
T9023 |
nmod |
cat.,H |
R2713 |
T9035 |
T9023 |
punct |
#,H |
R2714 |
T9036 |
T9023 |
compound |
NB730,H |
R2715 |
T9037 |
T9023 |
punct |
-,H |
R2716 |
T9038 |
T9023 |
punct |
),H |
R2717 |
T9039 |
T9017 |
auxpass |
was,used |
R2718 |
T9040 |
T9017 |
prep |
for,used |
R2719 |
T9041 |
T9040 |
pobj |
detection,for |
R2720 |
T9042 |
T9017 |
advcl |
using,used |
R2721 |
T9043 |
T9044 |
det |
the,reagent |
R2722 |
T9044 |
T9042 |
dobj |
reagent,using |
R2723 |
T9045 |
T9046 |
compound |
Western,Lightning |
R2724 |
T9046 |
T9044 |
compound |
Lightning,reagent |
R2725 |
T9047 |
T9044 |
compound |
chemiluminescence,reagent |
R2726 |
T9048 |
T9049 |
punct |
(,Elmer |
R2727 |
T9049 |
T9044 |
parataxis |
Elmer,reagent |
R2728 |
T9050 |
T9049 |
compound |
Perkin,Elmer |
R2729 |
T9051 |
T9049 |
punct |
", ",Elmer |
R2730 |
T9052 |
T9049 |
npadvmod |
Boston,Elmer |
R2731 |
T9053 |
T9049 |
punct |
", ",Elmer |
R2732 |
T9054 |
T9049 |
npadvmod |
MA,Elmer |
R2733 |
T9055 |
T9049 |
punct |
),Elmer |
R2734 |
T9056 |
T9017 |
cc |
and,used |
R2735 |
T9057 |
T9017 |
conj |
exposed,used |
R2736 |
T9058 |
T9057 |
prep |
to,exposed |
R2737 |
T9059 |
T9060 |
compound |
X-ray,film |
R2738 |
T9060 |
T9058 |
pobj |
film,to |
R2739 |
T9061 |
T9017 |
punct |
.,used |