PMC:1448208 / 14304-14642 JSONTXT

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    craft-sa-dev

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to determine the BCKDH activity. The use of tissue homogenates was necessitated by the limited availability of liver tissue, particularly from newborn pups. BCKDH activity was determined by measuring the release of 14CO2 from α-keto [1-14C] isocaproate as described previously [31]. The complete reaction mixture contained (final volu"}

    craft-ca-core-ex-dev

    {"project":"craft-ca-core-ex-dev","denotations":[{"id":"T8078","span":{"begin":48,"end":54},"obj":"UBERON:0000479"},{"id":"T8079","span":{"begin":115,"end":120},"obj":"UBERON:0002107"},{"id":"T8080","span":{"begin":121,"end":127},"obj":"UBERON:0000479"},{"id":"T8081","span":{"begin":147,"end":154},"obj":"PATO_UBERON_EXT:neonate_or_newborn"},{"id":"T8082","span":{"begin":155,"end":159},"obj":"NCBITaxon_UBERON_EXT:pup"},{"id":"T8083","span":{"begin":208,"end":215},"obj":"GO_EXT:biological_release"},{"id":"T8084","span":{"begin":221,"end":224},"obj":"CHEBI:16526"},{"id":"T8085","span":{"begin":230,"end":236},"obj":"_FRAGMENT"},{"id":"T8086","span":{"begin":245,"end":256},"obj":"CHEBI:17865"},{"id":"T8087","span":{"begin":240,"end":243},"obj":"CHEBI:36927"},{"id":"T8088","span":{"begin":309,"end":316},"obj":"CHEBI:60004"}],"relations":[{"id":"R2280","pred":"_lexicallyChainedTo","subj":"T8086","obj":"T8085"}],"text":"ved to determine the BCKDH activity. The use of tissue homogenates was necessitated by the limited availability of liver tissue, particularly from newborn pups. BCKDH activity was determined by measuring the release of 14CO2 from α-keto [1-14C] isocaproate as described previously [31]. The complete reaction mixture contained (final volu"}

    craft-ca-core-dev

    {"project":"craft-ca-core-dev","denotations":[{"id":"T8049","span":{"begin":48,"end":54},"obj":"UBERON:0000479"},{"id":"T8050","span":{"begin":115,"end":120},"obj":"UBERON:0002107"},{"id":"T8051","span":{"begin":121,"end":127},"obj":"UBERON:0000479"},{"id":"T8052","span":{"begin":221,"end":224},"obj":"CHEBI:16526"},{"id":"T8053","span":{"begin":230,"end":236},"obj":"_FRAGMENT"},{"id":"T8054","span":{"begin":245,"end":256},"obj":"CHEBI:17865"},{"id":"T8055","span":{"begin":240,"end":243},"obj":"CHEBI:36927"},{"id":"T8056","span":{"begin":309,"end":316},"obj":"CHEBI:60004"}],"relations":[{"id":"R2278","pred":"_lexicallyChainedTo","subj":"T8054","obj":"T8053"}],"text":"ved to determine the BCKDH activity. The use of tissue homogenates was necessitated by the limited availability of liver tissue, particularly from newborn pups. BCKDH activity was determined by measuring the release of 14CO2 from α-keto [1-14C] isocaproate as described previously [31]. The complete reaction mixture contained (final volu"}