PMC:1448208 / 12685-12941
Annnotations
craft-sa-dev
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Fibroblasts were passed onto glass, fixed in 2% paraformaldehyde in PBS for 10 minutes, permeabilized in 2% paraformaldehyde containing 0.1% Triton X100 for 10 minutes and washed three times in PBS containing 0.5% BSA and 0.15% glycine, pH 7.4 (Buffer A)."}
craft-ca-core-ex-dev
{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T7158","span":{"begin":1,"end":12},"obj":"CL:0000057"},{"id":"T7159","span":{"begin":49,"end":65},"obj":"CHEBI_EXT:paraformaldehye"},{"id":"T7160","span":{"begin":109,"end":125},"obj":"CHEBI_EXT:paraformaldehye"},{"id":"T7161","span":{"begin":142,"end":153},"obj":"CHEBI:9750"},{"id":"T7162","span":{"begin":229,"end":236},"obj":"CHEBI_SO_EXT:glycine"},{"id":"T7163","span":{"begin":246,"end":252},"obj":"CHEBI_CHMO_EXT:buffer_solution"}],"text":" Fibroblasts were passed onto glass, fixed in 2% paraformaldehyde in PBS for 10 minutes, permeabilized in 2% paraformaldehyde containing 0.1% Triton X100 for 10 minutes and washed three times in PBS containing 0.5% BSA and 0.15% glycine, pH 7.4 (Buffer A)."}
craft-ca-core-dev
{"project":"craft-ca-core-dev","denotations":[{"id":"T7145","span":{"begin":1,"end":12},"obj":"CL:0000057"},{"id":"T7146","span":{"begin":142,"end":153},"obj":"CHEBI:9750"}],"text":" Fibroblasts were passed onto glass, fixed in 2% paraformaldehyde in PBS for 10 minutes, permeabilized in 2% paraformaldehyde containing 0.1% Triton X100 for 10 minutes and washed three times in PBS containing 0.5% BSA and 0.15% glycine, pH 7.4 (Buffer A)."}