PMC:1440874 / 50062-56120 JSON TXT

Annnotations TAB JSON ListView MergeView

craft-sa-dev

Id	Subject	Object	Predicate	Lexical cue
T11189	23-33	NN	denotes	Generation
T11190	34-36	IN	denotes	of
T11191	37-39	NN	denotes	ES
T11192	40-45	NNS	denotes	cells
T11193	46-53	VBG	denotes	bearing
T11194	54-57	DT	denotes	the
T11195	68-74	NN	denotes	allele
T11196	58-67	NN	denotes	Atrx flox
T11197	74-75	.	denotes	.
T11198	75-240	sentence	denotes	Briefly, the targeting vector (shown in Figure 2A) places a loxP site within intron 18 and a loxP-flanked MC1neopA selection cassette in intron 17 of the Atrx gene.
T11199	76-83	RB	denotes	Briefly
T11200	127-133	VBZ	denotes	places
T11201	83-85	,	denotes	,
T11202	85-88	DT	denotes	the
T11203	99-105	NN	denotes	vector
T11204	89-98	NN	denotes	targeting
T11205	106-107	-LRB-	denotes	(
T11206	107-112	VBN	denotes	shown
T11207	113-115	IN	denotes	in
T11208	116-122	NN	denotes	Figure
T11209	123-125	NN	denotes	2A
T11210	125-126	-RRB-	denotes	)
T11211	134-135	DT	denotes	a
T11212	141-145	NN	denotes	site
T11213	136-140	NN	denotes	loxP
T11214	146-152	IN	denotes	within
T11215	153-159	NN	denotes	intron
T11216	160-162	CD	denotes	18
T11217	163-166	CC	denotes	and
T11218	167-168	DT	denotes	a
T11219	201-209	NN	denotes	cassette
T11220	169-173	NN	denotes	loxP
T11221	174-181	VBN	denotes	flanked
T11222	173-174	HYPH	denotes	-
T11223	182-190	NN	denotes	MC1neopA
T11224	191-200	NN	denotes	selection
T11225	210-212	IN	denotes	in
T11226	213-219	NN	denotes	intron
T11227	220-222	CD	denotes	17
T11228	223-225	IN	denotes	of
T11229	226-229	DT	denotes	the
T11230	235-239	NN	denotes	gene
T11231	230-234	NN	denotes	Atrx
T11232	239-240	.	denotes	.
T11233	240-311	sentence	denotes	A detailed description of the targeting construct is provided in [20].
T11234	241-242	DT	denotes	A
T11235	252-263	NN	denotes	description
T11236	243-251	VBN	denotes	detailed
T11237	294-302	VBN	denotes	provided
T11238	264-266	IN	denotes	of
T11239	267-270	DT	denotes	the
T11240	281-290	NN	denotes	construct
T11241	271-280	NN	denotes	targeting
T11242	291-293	VBZ	denotes	is
T11243	303-305	IN	denotes	in
T11244	306-307	-LRB-	denotes	[
T11245	307-309	CD	denotes	20
T11246	309-310	-RRB-	denotes	]
T11247	310-311	.	denotes	.
T11248	311-451	sentence	denotes	Linearised plasmid (150 μg) was electroporated into 1 × 108 E14Tg2a ES cells, and colonies resistant to G418 and ganciclovir were isolated.
T11249	312-322	VBN	denotes	Linearised
T11250	323-330	NN	denotes	plasmid
T11251	344-358	VBN	denotes	electroporated
T11252	331-332	-LRB-	denotes	(
T11253	336-338	NNS	denotes	μg
T11254	332-335	CD	denotes	150
T11255	338-339	-RRB-	denotes	)
T11256	340-343	VBD	denotes	was
T11257	359-363	IN	denotes	into
T11258	364-365	CD	denotes	1
T11259	368-371	CD	denotes	108
T11260	366-367	SYM	denotes	×
T11261	383-388	NNS	denotes	cells
T11262	372-379	NN	denotes	E14Tg2a
T11263	380-382	NN	denotes	ES
T11264	388-390	,	denotes	,
T11265	390-393	CC	denotes	and
T11266	394-402	NNS	denotes	colonies
T11267	442-450	VBN	denotes	isolated
T11268	403-412	JJ	denotes	resistant
T11269	413-415	IN	denotes	to
T11270	416-420	NN	denotes	G418
T11271	421-424	CC	denotes	and
T11272	425-436	NN	denotes	ganciclovir
T11273	437-441	VBD	denotes	were
T11274	450-451	.	denotes	.
T11275	451-685	sentence	denotes	Homologous targeting events were identified by Southern blot of EcoRI-digested DNA and hybridisation with a 5′ probe (generated with primers PPS1.20 and PPS1.27) and a 3′ probe (a 0.9-kb HaeIII fragment) as shown in Figure 2A and 2B.
T11276	452-462	JJ	denotes	Homologous
T11277	473-479	NNS	denotes	events
T11278	463-472	NN	denotes	targeting
T11279	485-495	VBN	denotes	identified
T11280	480-484	VBD	denotes	were
T11281	496-498	IN	denotes	by
T11282	499-507	NNP	denotes	Southern
T11283	508-512	NN	denotes	blot
T11284	513-515	IN	denotes	of
T11285	516-521	NN	denotes	EcoRI
T11286	522-530	VBN	denotes	digested
T11287	521-522	HYPH	denotes	-
T11288	531-534	NN	denotes	DNA
T11289	535-538	CC	denotes	and
T11290	539-552	NN	denotes	hybridisation
T11291	553-557	IN	denotes	with
T11292	558-559	DT	denotes	a
T11293	563-568	NN	denotes	probe
T11294	560-561	CD	denotes	5
T11295	561-562	SYM	denotes	′
T11296	569-570	-LRB-	denotes	(
T11297	570-579	VBN	denotes	generated
T11298	580-584	IN	denotes	with
T11299	585-592	NNS	denotes	primers
T11300	593-600	NN	denotes	PPS1.20
T11301	601-604	CC	denotes	and
T11302	605-612	NN	denotes	PPS1.27
T11303	612-613	-RRB-	denotes	)
T11304	614-617	CC	denotes	and
T11305	618-619	DT	denotes	a
T11306	623-628	NN	denotes	probe
T11307	620-621	CD	denotes	3
T11308	621-622	SYM	denotes	′
T11309	629-630	-LRB-	denotes	(
T11310	630-631	DT	denotes	a
T11311	646-654	NN	denotes	fragment
T11312	632-635	CD	denotes	0.9
T11313	636-638	NN	denotes	kb
T11314	635-636	HYPH	denotes	-
T11315	639-645	NN	denotes	HaeIII
T11316	654-655	-RRB-	denotes	)
T11317	656-658	IN	denotes	as
T11318	659-664	VBN	denotes	shown
T11319	665-667	IN	denotes	in
T11320	668-674	NN	denotes	Figure
T11321	675-677	NN	denotes	2A
T11322	678-681	CC	denotes	and
T11323	682-684	NN	denotes	2B
T11324	684-685	.	denotes	.
T11325	685-1004	sentence	denotes	DNA from correctly targeted clones was also digested with SacI and analysed by Southern blot and hybridisation with a probe from within intron 17 (a PCR product generated with primers PPS1.15 and Xnp46) to confirm that the loxP site within intron 18 had been included within the crossed-over region (Figure 2A and 2C).
T11326	686-689	NN	denotes	DNA
T11327	730-738	VBN	denotes	digested
T11328	690-694	IN	denotes	from
T11329	695-704	RB	denotes	correctly
T11330	705-713	VBN	denotes	targeted
T11331	714-720	NNS	denotes	clones
T11332	721-724	VBD	denotes	was
T11333	725-729	RB	denotes	also
T11334	739-743	IN	denotes	with
T11335	744-748	NN	denotes	SacI
T11336	749-752	CC	denotes	and
T11337	753-761	VBN	denotes	analysed
T11338	762-764	IN	denotes	by
T11339	765-773	NNP	denotes	Southern
T11340	774-778	NN	denotes	blot
T11341	779-782	CC	denotes	and
T11342	783-796	NN	denotes	hybridisation
T11343	797-801	IN	denotes	with
T11344	802-803	DT	denotes	a
T11345	804-809	NN	denotes	probe
T11346	810-814	IN	denotes	from
T11347	815-821	IN	denotes	within
T11348	822-828	NN	denotes	intron
T11349	829-831	CD	denotes	17
T11350	832-833	-LRB-	denotes	(
T11351	833-834	DT	denotes	a
T11352	839-846	NN	denotes	product
T11353	835-838	NN	denotes	PCR
T11354	847-856	VBN	denotes	generated
T11355	857-861	IN	denotes	with
T11356	862-869	NNS	denotes	primers
T11357	870-877	NN	denotes	PPS1.15
T11358	878-881	CC	denotes	and
T11359	882-887	NN	denotes	Xnp46
T11360	887-888	-RRB-	denotes	)
T11361	889-891	TO	denotes	to
T11362	892-899	VB	denotes	confirm
T11363	900-904	IN	denotes	that
T11364	945-953	VBN	denotes	included
T11365	905-908	DT	denotes	the
T11366	914-918	NN	denotes	site
T11367	909-913	NN	denotes	loxP
T11368	919-925	IN	denotes	within
T11369	926-932	NN	denotes	intron
T11370	933-935	CD	denotes	18
T11371	936-939	VBD	denotes	had
T11372	940-944	VBN	denotes	been
T11373	954-960	IN	denotes	within
T11374	961-964	DT	denotes	the
T11375	978-984	NN	denotes	region
T11376	965-972	VBN	denotes	crossed
T11377	972-973	HYPH	denotes	-
T11378	973-977	RP	denotes	over
T11379	985-986	-LRB-	denotes	(
T11380	993-995	NN	denotes	2A
T11381	986-992	NN	denotes	Figure
T11382	996-999	CC	denotes	and
T11383	1000-1002	NN	denotes	2C
T11384	1002-1003	-RRB-	denotes	)
T11385	1003-1004	.	denotes	.
T11386	1004-1048	sentence	denotes	Sequences of primers are shown in Table S1.
T11387	1005-1014	NNS	denotes	Sequences
T11388	1030-1035	VBN	denotes	shown
T11389	1015-1017	IN	denotes	of
T11390	1018-1025	NNS	denotes	primers
T11391	1026-1029	VBP	denotes	are
T11392	1036-1038	IN	denotes	in
T11393	1039-1044	NN	denotes	Table
T11394	1045-1047	NN	denotes	S1
T11395	1047-1048	.	denotes	.
T11569	1050-1053	NN	denotes	Cre
T11570	1054-1067	NN	denotes	recombination
T11571	1053-1054	HYPH	denotes	-
T11572	1068-1071	CC	denotes	and
T11573	1072-1088	NN	denotes	characterisation
T11574	1089-1091	IN	denotes	of
T11575	1092-1100	JJ	denotes	Atrxnull
T11576	1104-1109	NNS	denotes	cells
T11577	1101-1103	NN	denotes	ES
T11578	1110-1113	CC	denotes	and
T11579	1114-1122	JJ	denotes	embryoid
T11580	1123-1129	NNS	denotes	bodies
T11581	1129-1130	.	denotes	.
T11582	1130-1290	sentence	denotes	ES cell clones bearing the Atrx flox allele (1 × 107 cells) were transiently transfected with 50 μg of uncut Cre expression plasmid (pCAGGS-Cre-IRESpuro) [40].
T11583	1131-1133	NN	denotes	ES
T11584	1134-1138	NN	denotes	cell
T11585	1139-1145	NNS	denotes	clones
T11586	1208-1219	VBN	denotes	transfected
T11587	1146-1153	VBG	denotes	bearing
T11588	1154-1157	DT	denotes	the
T11589	1168-1174	NN	denotes	allele
T11590	1158-1167	NN	denotes	Atrx flox
T11591	1175-1176	-LRB-	denotes	(
T11592	1184-1189	NNS	denotes	cells
T11593	1176-1177	CD	denotes	1
T11594	1180-1183	CD	denotes	107
T11595	1178-1179	SYM	denotes	×
T11596	1189-1190	-RRB-	denotes	)
T11597	1191-1195	VBD	denotes	were
T11598	1196-1207	RB	denotes	transiently
T11599	1220-1224	IN	denotes	with
T11600	1225-1227	CD	denotes	50
T11601	1228-1230	NNS	denotes	μg
T11602	1231-1233	IN	denotes	of
T11603	1234-1239	JJ	denotes	uncut
T11604	1255-1262	NN	denotes	plasmid
T11605	1240-1243	NN	denotes	Cre
T11606	1244-1254	NN	denotes	expression
T11607	1263-1264	-LRB-	denotes	(
T11608	1264-1270	NN	denotes	pCAGGS
T11609	1275-1283	NN	denotes	IRESpuro
T11610	1270-1271	HYPH	denotes	-
T11611	1271-1274	NN	denotes	Cre
T11612	1274-1275	HYPH	denotes	-
T11613	1283-1284	-RRB-	denotes	)
T11614	1285-1286	-LRB-	denotes	[
T11615	1286-1288	CD	denotes	40
T11616	1288-1289	-RRB-	denotes	]
T11617	1289-1290	.	denotes	.
T11618	1290-1442	sentence	denotes	Following transfection, cells were plated at a range of clonal densities in complete medium without G418, and isolated subclones were picked after 7 d.
T11619	1291-1300	VBG	denotes	Following
T11620	1326-1332	VBN	denotes	plated
T11621	1301-1313	NN	denotes	transfection
T11622	1313-1315	,	denotes	,
T11623	1315-1320	NNS	denotes	cells
T11624	1321-1325	VBD	denotes	were
T11625	1333-1335	IN	denotes	at
T11626	1336-1337	DT	denotes	a
T11627	1338-1343	NN	denotes	range
T11628	1344-1346	IN	denotes	of
T11629	1347-1353	JJ	denotes	clonal
T11630	1354-1363	NNS	denotes	densities
T11631	1364-1366	IN	denotes	in
T11632	1367-1375	JJ	denotes	complete
T11633	1376-1382	NN	denotes	medium
T11634	1383-1390	IN	denotes	without
T11635	1391-1395	NN	denotes	G418
T11636	1395-1397	,	denotes	,
T11637	1397-1400	CC	denotes	and
T11638	1401-1409	VBN	denotes	isolated
T11639	1410-1419	NNS	denotes	subclones
T11640	1425-1431	VBN	denotes	picked
T11641	1420-1424	VBD	denotes	were
T11642	1432-1437	IN	denotes	after
T11643	1438-1439	CD	denotes	7
T11644	1440-1441	NNS	denotes	d
T11645	1441-1442	.	denotes	.
T11646	1442-1687	sentence	denotes	Subclones were expanded and analysed for the presence of a recombinant locus initially by PCR, to detect deletion of the MC1neopA cassette, and then by Southern blot and hybridisation with the intron 17 probe described above (Figure 2A and 2C).
T11647	1443-1452	NNS	denotes	Subclones
T11648	1458-1466	VBN	denotes	expanded
T11649	1453-1457	VBD	denotes	were
T11650	1467-1470	CC	denotes	and
T11651	1471-1479	VBN	denotes	analysed
T11652	1480-1483	IN	denotes	for
T11653	1484-1487	DT	denotes	the
T11654	1488-1496	NN	denotes	presence
T11655	1497-1499	IN	denotes	of
T11656	1500-1501	DT	denotes	a
T11657	1514-1519	NN	denotes	locus
T11658	1502-1513	JJ	denotes	recombinant
T11659	1520-1529	RB	denotes	initially
T11660	1530-1532	IN	denotes	by
T11661	1533-1536	NN	denotes	PCR
T11662	1536-1538	,	denotes	,
T11663	1538-1540	TO	denotes	to
T11664	1541-1547	VB	denotes	detect
T11665	1548-1556	NN	denotes	deletion
T11666	1557-1559	IN	denotes	of
T11667	1560-1563	DT	denotes	the
T11668	1573-1581	NN	denotes	cassette
T11669	1564-1572	NN	denotes	MC1neopA
T11670	1581-1583	,	denotes	,
T11671	1583-1586	CC	denotes	and
T11672	1587-1591	RB	denotes	then
T11673	1592-1594	IN	denotes	by
T11674	1595-1603	NNP	denotes	Southern
T11675	1604-1608	NN	denotes	blot
T11676	1609-1612	CC	denotes	and
T11677	1613-1626	NN	denotes	hybridisation
T11678	1627-1631	IN	denotes	with
T11679	1632-1635	DT	denotes	the
T11680	1646-1651	NN	denotes	probe
T11681	1636-1642	NN	denotes	intron
T11682	1643-1645	CD	denotes	17
T11683	1652-1661	VBN	denotes	described
T11684	1662-1667	RB	denotes	above
T11685	1668-1669	-LRB-	denotes	(
T11686	1676-1678	NN	denotes	2A
T11687	1669-1675	NN	denotes	Figure
T11688	1679-1682	CC	denotes	and
T11689	1683-1685	NN	denotes	2C
T11690	1685-1686	-RRB-	denotes	)
T11691	1686-1687	.	denotes	.
T11692	1687-1857	sentence	denotes	Northern blots were carried out according to standard techniques using 20 μg of total RNA isolated using TRI Reagent (Sigma-Aldrich, St. Louis, Missouri, United States).
T11693	1688-1696	NNP	denotes	Northern
T11694	1697-1702	NNS	denotes	blots
T11695	1708-1715	VBN	denotes	carried
T11696	1703-1707	VBD	denotes	were
T11697	1716-1719	RP	denotes	out
T11698	1720-1729	VBG	denotes	according
T11699	1730-1732	IN	denotes	to
T11700	1733-1741	JJ	denotes	standard
T11701	1742-1752	NNS	denotes	techniques
T11702	1753-1758	VBG	denotes	using
T11703	1759-1761	CD	denotes	20
T11704	1762-1764	NNS	denotes	μg
T11705	1765-1767	IN	denotes	of
T11706	1768-1773	JJ	denotes	total
T11707	1774-1777	NN	denotes	RNA
T11708	1778-1786	VBN	denotes	isolated
T11709	1787-1792	VBG	denotes	using
T11710	1793-1796	NN	denotes	TRI
T11711	1797-1804	NN	denotes	Reagent
T11712	1805-1806	-LRB-	denotes	(
T11713	1812-1819	NNP	denotes	Aldrich
T11714	1806-1811	NNP	denotes	Sigma
T11715	1811-1812	HYPH	denotes	-
T11716	1819-1821	,	denotes	,
T11717	1821-1824	NNP	denotes	St.
T11718	1825-1830	NNP	denotes	Louis
T11719	1830-1832	,	denotes	,
T11720	1832-1840	NNP	denotes	Missouri
T11721	1840-1842	,	denotes	,
T11722	1842-1848	NNP	denotes	United
T11723	1849-1855	NNP	denotes	States
T11724	1855-1856	-RRB-	denotes	)
T11725	1856-1857	.	denotes	.
T11726	1857-1985	sentence	denotes	The blot was hybridised with a probe from within exon 10 of the Atrx gene (generated with primers Mxnp4 and Mxnp28 [Table S1]).
T11727	1858-1861	DT	denotes	The
T11728	1862-1866	NN	denotes	blot
T11729	1871-1881	VBN	denotes	hybridised
T11730	1867-1870	VBD	denotes	was
T11731	1882-1886	IN	denotes	with
T11732	1887-1888	DT	denotes	a
T11733	1889-1894	NN	denotes	probe
T11734	1895-1899	IN	denotes	from
T11735	1900-1906	IN	denotes	within
T11736	1907-1911	NN	denotes	exon
T11737	1912-1914	CD	denotes	10
T11738	1915-1917	IN	denotes	of
T11739	1918-1921	DT	denotes	the
T11740	1927-1931	NN	denotes	gene
T11741	1922-1926	NN	denotes	Atrx
T11742	1932-1933	-LRB-	denotes	(
T11743	1933-1942	VBN	denotes	generated
T11744	1943-1947	IN	denotes	with
T11745	1948-1955	NNS	denotes	primers
T11746	1956-1961	NN	denotes	Mxnp4
T11747	1962-1965	CC	denotes	and
T11748	1966-1972	NN	denotes	Mxnp28
T11749	1973-1974	-LRB-	denotes	[
T11750	1980-1982	NN	denotes	S1
T11751	1974-1979	NN	denotes	Table
T11752	1982-1983	-RRB-	denotes	]
T11753	1983-1984	-RRB-	denotes	)
T11754	1984-1985	.	denotes	.
T11755	1985-2112	sentence	denotes	After it was stripped, the membrane was hybridised with a β-actin cDNA probe (Clontech, Palo Alto, California, United States).
T11756	1986-1991	IN	denotes	After
T11757	1999-2007	VBN	denotes	stripped
T11758	1992-1994	PRP	denotes	it
T11759	1995-1998	VBD	denotes	was
T11760	2026-2036	VBN	denotes	hybridised
T11761	2007-2009	,	denotes	,
T11762	2009-2012	DT	denotes	the
T11763	2013-2021	NN	denotes	membrane
T11764	2022-2025	VBD	denotes	was
T11765	2037-2041	IN	denotes	with
T11766	2042-2043	DT	denotes	a
T11767	2057-2062	NN	denotes	probe
T11768	2044-2045	NN	denotes	β
T11769	2046-2051	NN	denotes	actin
T11770	2045-2046	HYPH	denotes	-
T11771	2052-2056	NN	denotes	cDNA
T11772	2063-2064	-LRB-	denotes	(
T11773	2064-2072	NNP	denotes	Clontech
T11774	2072-2074	,	denotes	,
T11775	2074-2078	NNP	denotes	Palo
T11776	2079-2083	NNP	denotes	Alto
T11777	2083-2085	,	denotes	,
T11778	2085-2095	NNP	denotes	California
T11779	2095-2097	,	denotes	,
T11780	2097-2103	NNP	denotes	United
T11781	2104-2110	NNP	denotes	States
T11782	2110-2111	-RRB-	denotes	)
T11783	2111-2112	.	denotes	.
T11784	2112-2272	sentence	denotes	Protein extraction and detection of Atrx by Western blotting was performed as described previously [4], using the mouse monoclonal anti-ATRX antibody 23C [15].
T11785	2113-2120	NN	denotes	Protein
T11786	2121-2131	NN	denotes	extraction
T11787	2178-2187	VBN	denotes	performed
T11788	2132-2135	CC	denotes	and
T11789	2136-2145	NN	denotes	detection
T11790	2146-2148	IN	denotes	of
T11791	2149-2153	NN	denotes	Atrx
T11792	2154-2156	IN	denotes	by
T11793	2157-2164	NNP	denotes	Western
T11794	2165-2173	NN	denotes	blotting
T11795	2174-2177	VBD	denotes	was
T11796	2188-2190	IN	denotes	as
T11797	2191-2200	VBN	denotes	described
T11798	2201-2211	RB	denotes	previously
T11799	2212-2213	-LRB-	denotes	[
T11800	2213-2214	CD	denotes	4
T11801	2214-2215	-RRB-	denotes	]
T11802	2215-2217	,	denotes	,
T11803	2217-2222	VBG	denotes	using
T11804	2223-2226	DT	denotes	the
T11805	2254-2262	NN	denotes	antibody
T11806	2227-2232	NN	denotes	mouse
T11807	2233-2243	JJ	denotes	monoclonal
T11808	2244-2253	JJ	denotes	anti-ATRX
T11809	2263-2266	NN	denotes	23C
T11810	2267-2268	-LRB-	denotes	[
T11811	2268-2270	CD	denotes	15
T11812	2270-2271	-RRB-	denotes	]
T11813	2271-2272	.	denotes	.
T11814	2272-2339	sentence	denotes	Analyses of cell cycle and apoptosis are described in Protocol S1.
T11815	2273-2281	NNS	denotes	Analyses
T11816	2314-2323	VBN	denotes	described
T11817	2282-2284	IN	denotes	of
T11818	2285-2289	NN	denotes	cell
T11819	2290-2295	NN	denotes	cycle
T11820	2296-2299	CC	denotes	and
T11821	2300-2309	NN	denotes	apoptosis
T11822	2310-2313	VBP	denotes	are
T11823	2324-2326	IN	denotes	in
T11824	2327-2335	NN	denotes	Protocol
T11825	2336-2338	NN	denotes	S1
T11826	2338-2339	.	denotes	.
T11827	2339-2501	sentence	denotes	Methylation of rDNA was analysed in DNA from ES cell clones or from embryoid bodies recovered after 7 d of in vitro differentiation as described previously [41].
T11828	2340-2351	NN	denotes	Methylation
T11829	2364-2372	VBN	denotes	analysed
T11830	2352-2354	IN	denotes	of
T11831	2355-2359	NN	denotes	rDNA
T11832	2360-2363	VBD	denotes	was
T11833	2373-2375	IN	denotes	in
T11834	2376-2379	NN	denotes	DNA
T11835	2380-2384	IN	denotes	from
T11836	2385-2387	NN	denotes	ES
T11837	2388-2392	NN	denotes	cell
T11838	2393-2399	NNS	denotes	clones
T11839	2400-2402	CC	denotes	or
T11840	2403-2407	IN	denotes	from
T11841	2408-2416	JJ	denotes	embryoid
T11842	2417-2423	NNS	denotes	bodies
T11843	2424-2433	VBN	denotes	recovered
T11844	2434-2439	IN	denotes	after
T11845	2440-2441	CD	denotes	7
T11846	2442-2443	NNS	denotes	d
T11847	2444-2446	IN	denotes	of
T11848	2447-2449	FW	denotes	in
T11849	2450-2455	FW	denotes	vitro
T11850	2456-2471	NN	denotes	differentiation
T11851	2472-2474	IN	denotes	as
T11852	2475-2484	VBN	denotes	described
T11853	2485-2495	RB	denotes	previously
T11854	2496-2497	-LRB-	denotes	[
T11855	2497-2499	CD	denotes	41
T11856	2499-2500	-RRB-	denotes	]
T11857	2500-2501	.	denotes	.
T11858	2501-2621	sentence	denotes	Genomic DNA was digested with methylation-sensitive restriction enzymes as described and analysed by Southern blotting.
T11859	2502-2509	JJ	denotes	Genomic
T11860	2510-2513	NN	denotes	DNA
T11861	2518-2526	VBN	denotes	digested
T11862	2514-2517	VBD	denotes	was
T11863	2527-2531	IN	denotes	with
T11864	2532-2543	NN	denotes	methylation
T11865	2544-2553	JJ	denotes	sensitive
T11866	2543-2544	HYPH	denotes	-
T11867	2566-2573	NNS	denotes	enzymes
T11868	2554-2565	NN	denotes	restriction
T11869	2574-2576	IN	denotes	as
T11870	2577-2586	VBN	denotes	described
T11871	2587-2590	CC	denotes	and
T11872	2591-2599	VBN	denotes	analysed
T11873	2600-2602	IN	denotes	by
T11874	2603-2611	NNP	denotes	Southern
T11875	2612-2620	NN	denotes	blotting
T11876	2620-2621	.	denotes	.
T11877	2621-2765	sentence	denotes	The RIB3 and RIB4 probes (which were amplified from human DNA, but cross-react with the mouse rDNA repeat) have been described previously [11].
T11878	2622-2625	DT	denotes	The
T11879	2640-2646	NNS	denotes	probes
T11880	2626-2630	NN	denotes	RIB3
T11881	2631-2634	CC	denotes	and
T11882	2635-2639	NN	denotes	RIB4
T11883	2739-2748	VBN	denotes	described
T11884	2647-2648	-LRB-	denotes	(
T11885	2648-2653	WDT	denotes	which
T11886	2659-2668	VBN	denotes	amplified
T11887	2654-2658	VBD	denotes	were
T11888	2669-2673	IN	denotes	from
T11889	2674-2679	JJ	denotes	human
T11890	2680-2683	NN	denotes	DNA
T11891	2683-2685	,	denotes	,
T11892	2685-2688	CC	denotes	but
T11893	2689-2700	VBP	denotes	cross-react
T11894	2701-2705	IN	denotes	with
T11895	2706-2709	DT	denotes	the
T11896	2721-2727	NN	denotes	repeat
T11897	2710-2715	NN	denotes	mouse
T11898	2716-2720	NN	denotes	rDNA
T11899	2727-2728	-RRB-	denotes	)
T11900	2729-2733	VBP	denotes	have
T11901	2734-2738	VBN	denotes	been
T11902	2749-2759	RB	denotes	previously
T11903	2760-2761	-LRB-	denotes	[
T11904	2761-2763	CD	denotes	11
T11905	2763-2764	-RRB-	denotes	]
T11906	2764-2765	.	denotes	.
T11907	2765-2862	sentence	denotes	Oligonucleotide probes to detect Line 1 and Sine B1 repeats have been described previously [42].
T11908	2766-2781	NN	denotes	Oligonucleotide
T11909	2782-2788	NNS	denotes	probes
T11910	2836-2845	VBN	denotes	described
T11911	2789-2791	TO	denotes	to
T11912	2792-2798	VB	denotes	detect
T11913	2799-2803	NN	denotes	Line
T11914	2818-2825	NNS	denotes	repeats
T11915	2804-2805	CD	denotes	1
T11916	2806-2809	CC	denotes	and
T11917	2810-2814	NN	denotes	Sine
T11918	2815-2817	NN	denotes	B1
T11919	2826-2830	VBP	denotes	have
T11920	2831-2835	VBN	denotes	been
T11921	2846-2856	RB	denotes	previously
T11922	2857-2858	-LRB-	denotes	[
T11923	2858-2860	CD	denotes	42
T11924	2860-2861	-RRB-	denotes	]
T11925	2861-2862	.	denotes	.
T11926	2862-2927	sentence	denotes	The minor satellite probe was a 27-mer oligonucleotide (mCENT2).
T11927	2863-2866	DT	denotes	The
T11928	2883-2888	NN	denotes	probe
T11929	2867-2872	JJ	denotes	minor
T11930	2873-2882	NN	denotes	satellite
T11931	2889-2892	VBD	denotes	was
T11932	2893-2894	DT	denotes	a
T11933	2902-2917	NN	denotes	oligonucleotide
T11934	2895-2897	CD	denotes	27
T11935	2898-2901	NN	denotes	mer
T11936	2897-2898	HYPH	denotes	-
T11937	2918-2919	-LRB-	denotes	(
T11938	2919-2925	NN	denotes	mCENT2
T11939	2925-2926	-RRB-	denotes	)
T11940	2926-2927	.	denotes	.
T11941	2927-2990	sentence	denotes	The major satellite probe was a 27-mer oligonucleotide (DG27).
T11942	2928-2931	DT	denotes	The
T11943	2948-2953	NN	denotes	probe
T11944	2932-2937	JJ	denotes	major
T11945	2938-2947	NN	denotes	satellite
T11946	2954-2957	VBD	denotes	was
T11947	2958-2959	DT	denotes	a
T11948	2967-2982	NN	denotes	oligonucleotide
T11949	2960-2962	CD	denotes	27
T11950	2963-2966	NN	denotes	mer
T11951	2962-2963	HYPH	denotes	-
T11952	2983-2984	-LRB-	denotes	(
T11953	2984-2988	NN	denotes	DG27
T11954	2988-2989	-RRB-	denotes	)
T11955	2989-2990	.	denotes	.
T11956	2990-3167	sentence	denotes	The IAP probe was an ~400 bp PCR product (primers 14A and 13K) amplified from an IAP inserted into the mouse agouti gene [43] and was a gift from Peter Warnecke and Tim Bestor.
T11957	2991-2994	DT	denotes	The
T11958	2999-3004	NN	denotes	probe
T11959	2995-2998	NN	denotes	IAP
T11960	3005-3008	VBD	denotes	was
T11961	3009-3011	DT	denotes	an
T11962	3024-3031	NN	denotes	product
T11963	3012-3013	SYM	denotes	~
T11964	3013-3016	CD	denotes	400
T11965	3017-3019	NN	denotes	bp
T11966	3020-3023	NN	denotes	PCR
T11967	3032-3033	-LRB-	denotes	(
T11968	3033-3040	NNS	denotes	primers
T11969	3041-3044	NN	denotes	14A
T11970	3045-3048	CC	denotes	and
T11971	3049-3052	NN	denotes	13K
T11972	3052-3053	-RRB-	denotes	)
T11973	3054-3063	VBN	denotes	amplified
T11974	3064-3068	IN	denotes	from
T11975	3069-3071	DT	denotes	an
T11976	3072-3075	NN	denotes	IAP
T11977	3076-3084	VBN	denotes	inserted
T11978	3085-3089	IN	denotes	into
T11979	3090-3093	DT	denotes	the
T11980	3107-3111	NN	denotes	gene
T11981	3094-3099	NN	denotes	mouse
T11982	3100-3106	NN	denotes	agouti
T11983	3112-3113	-LRB-	denotes	[
T11984	3113-3115	CD	denotes	43
T11985	3115-3116	-RRB-	denotes	]
T11986	3117-3120	CC	denotes	and
T11987	3121-3124	VBD	denotes	was
T11988	3125-3126	DT	denotes	a
T11989	3127-3131	NN	denotes	gift
T11990	3132-3136	IN	denotes	from
T11991	3137-3142	NNP	denotes	Peter
T11992	3143-3151	NNP	denotes	Warnecke
T11993	3152-3155	CC	denotes	and
T11994	3156-3159	NNP	denotes	Tim
T11995	3160-3166	NNP	denotes	Bestor
T11996	3166-3167	.	denotes	.
T11997	3167-3222	sentence	denotes	The PCR product included the entire 5′ LTR of the IAP.
T11998	3168-3171	DT	denotes	The
T11999	3176-3183	NN	denotes	product
T12000	3172-3175	NN	denotes	PCR
T12001	3184-3192	VBD	denotes	included
T12002	3193-3196	DT	denotes	the
T12003	3207-3210	NN	denotes	LTR
T12004	3197-3203	JJ	denotes	entire
T12005	3204-3205	CD	denotes	5
T12006	3205-3206	SYM	denotes	′
T12007	3211-3213	IN	denotes	of
T12008	3214-3217	DT	denotes	the
T12009	3218-3221	NN	denotes	IAP
T12010	3221-3222	.	denotes	.
T12011	3222-3275	sentence	denotes	All oligonucleotide sequences are shown in Table S1.
T12012	3223-3226	DT	denotes	All
T12013	3243-3252	NNS	denotes	sequences
T12014	3227-3242	NN	denotes	oligonucleotide
T12015	3257-3262	VBN	denotes	shown
T12016	3253-3256	VBP	denotes	are
T12017	3263-3265	IN	denotes	in
T12018	3266-3271	NN	denotes	Table
T12019	3272-3274	NN	denotes	S1
T12020	3274-3275	.	denotes	.
T12080	3277-3287	NN	denotes	Generation
T12081	3288-3290	IN	denotes	of
T12082	3291-3299	NNS	denotes	chimeras
T12083	3299-3301	,	denotes	,
T12084	3301-3307	VBN	denotes	floxed
T12085	3308-3312	NNS	denotes	mice
T12086	3312-3314	,	denotes	,
T12087	3314-3317	CC	denotes	and
T12088	3318-3324	NN	denotes	mutant
T12089	3325-3329	NNS	denotes	mice
T12090	3329-3330	.	denotes	.
T12091	3330-3482	sentence	denotes	Targeted Atrx flox ES cell clones were injected into C57BL/6 blastocysts and transferred into 2.5 dpc pseudopregnant recipients by standard techniques.
T12092	3331-3339	VBN	denotes	Targeted
T12093	3358-3364	NNS	denotes	clones
T12094	3340-3349	NN	denotes	Atrx flox
T12095	3353-3357	NN	denotes	cell
T12096	3350-3352	NN	denotes	ES
T12097	3370-3378	VBN	denotes	injected
T12098	3365-3369	VBD	denotes	were
T12099	3379-3383	IN	denotes	into
T12100	3384-3389	NN	denotes	C57BL
T12101	3392-3403	NNS	denotes	blastocysts
T12102	3389-3390	HYPH	denotes	/
T12103	3390-3391	CD	denotes	6
T12104	3404-3407	CC	denotes	and
T12105	3408-3419	VBN	denotes	transferred
T12106	3420-3424	IN	denotes	into
T12107	3425-3428	CD	denotes	2.5
T12108	3429-3432	NN	denotes	dpc
T12109	3448-3458	NNS	denotes	recipients
T12110	3433-3447	JJ	denotes	pseudopregnant
T12111	3459-3461	IN	denotes	by
T12112	3462-3470	JJ	denotes	standard
T12113	3471-3481	NNS	denotes	techniques
T12114	3481-3482	.	denotes	.
T12115	3482-3561	sentence	denotes	Resulting chimeras were mated with C57BL/6 to establish germline transmission.
T12116	3483-3492	VBG	denotes	Resulting
T12117	3493-3501	NNS	denotes	chimeras
T12118	3507-3512	VBN	denotes	mated
T12119	3502-3506	VBD	denotes	were
T12120	3513-3517	IN	denotes	with
T12121	3518-3523	NN	denotes	C57BL
T12122	3523-3524	HYPH	denotes	/
T12123	3524-3525	CD	denotes	6
T12124	3526-3528	TO	denotes	to
T12125	3529-3538	VB	denotes	establish
T12126	3539-3547	NN	denotes	germline
T12127	3548-3560	NN	denotes	transmission
T12128	3560-3561	.	denotes	.
T12129	3561-3716	sentence	denotes	Offspring with the Atrx flox allele were identified by Southern blotting of SacI-digested tail DNA using the intron 17 probe as shown in Figure 2A and 2C.
T12130	3562-3571	NN	denotes	Offspring
T12131	3603-3613	VBN	denotes	identified
T12132	3572-3576	IN	denotes	with
T12133	3577-3580	DT	denotes	the
T12134	3591-3597	NN	denotes	allele
T12135	3581-3590	NN	denotes	Atrx flox
T12136	3598-3602	VBD	denotes	were
T12137	3614-3616	IN	denotes	by
T12138	3617-3625	NNP	denotes	Southern
T12139	3626-3634	NN	denotes	blotting
T12140	3635-3637	IN	denotes	of
T12141	3638-3642	NN	denotes	SacI
T12142	3643-3651	VBN	denotes	digested
T12143	3642-3643	HYPH	denotes	-
T12144	3657-3660	NN	denotes	DNA
T12145	3652-3656	NN	denotes	tail
T12146	3661-3666	VBG	denotes	using
T12147	3667-3670	DT	denotes	the
T12148	3681-3686	NN	denotes	probe
T12149	3671-3677	NN	denotes	intron
T12150	3678-3680	CD	denotes	17
T12151	3687-3689	IN	denotes	as
T12152	3690-3695	VBN	denotes	shown
T12153	3696-3698	IN	denotes	in
T12154	3699-3705	NN	denotes	Figure
T12155	3706-3708	NN	denotes	2A
T12156	3709-3712	CC	denotes	and
T12157	3713-3715	NN	denotes	2C
T12158	3715-3716	.	denotes	.
T12159	3716-3824	sentence	denotes	For Cre-recombination, Atrx flox mice were crossed with GATA1-Cre transgenic mice as described in the text.
T12160	3717-3720	IN	denotes	For
T12161	3760-3767	VBN	denotes	crossed
T12162	3721-3724	NN	denotes	Cre
T12163	3725-3738	NN	denotes	recombination
T12164	3724-3725	HYPH	denotes	-
T12165	3738-3740	,	denotes	,
T12166	3740-3749	NN	denotes	Atrx flox
T12167	3750-3754	NNS	denotes	mice
T12168	3755-3759	VBD	denotes	were
T12169	3768-3772	IN	denotes	with
T12170	3773-3778	NN	denotes	GATA1
T12171	3779-3782	NN	denotes	Cre
T12172	3778-3779	HYPH	denotes	-
T12173	3783-3793	JJ	denotes	transgenic
T12174	3794-3798	NNS	denotes	mice
T12175	3799-3801	IN	denotes	as
T12176	3802-3811	VBN	denotes	described
T12177	3812-3814	IN	denotes	in
T12178	3815-3818	DT	denotes	the
T12179	3819-3823	NN	denotes	text
T12180	3823-3824	.	denotes	.
T12181	3824-3937	sentence	denotes	Recombinant alleles were detected by Southern blotting as described above or by PCR as described in Protocol S1.
T12182	3825-3836	JJ	denotes	Recombinant
T12183	3837-3844	NNS	denotes	alleles
T12184	3850-3858	VBN	denotes	detected
T12185	3845-3849	VBD	denotes	were
T12186	3859-3861	IN	denotes	by
T12187	3862-3870	NNP	denotes	Southern
T12188	3871-3879	NN	denotes	blotting
T12189	3880-3882	IN	denotes	as
T12190	3883-3892	VBN	denotes	described
T12191	3893-3898	RB	denotes	above
T12192	3899-3901	CC	denotes	or
T12193	3902-3904	IN	denotes	by
T12194	3905-3908	NN	denotes	PCR
T12195	3909-3911	IN	denotes	as
T12196	3912-3921	VBN	denotes	described
T12197	3922-3924	IN	denotes	in
T12198	3925-3933	NN	denotes	Protocol
T12199	3934-3936	NN	denotes	S1
T12200	3936-3937	.	denotes	.
T12292	3939-3959	NN	denotes	Immunohistochemistry
T12293	3959-3961	,	denotes	,
T12294	3961-3963	FW	denotes	in
T12295	3964-3968	FW	denotes	situ
T12296	3969-3982	NN	denotes	hybridisation
T12297	3982-3984	,	denotes	,
T12298	3984-3987	CC	denotes	and
T12299	3988-3993	NN	denotes	TUNEL
T12300	3994-3999	NN	denotes	assay
T12301	3999-4000	.	denotes	.
T12302	4000-4124	sentence	denotes	7.5 dpc decidual swellings were dissected away from maternal tissue and fixed in 4% paraformaldehyde/PBS overnight at 4 °C.
T12303	4001-4004	CD	denotes	7.5
T12304	4005-4008	NN	denotes	dpc
T12305	4018-4027	NNS	denotes	swellings
T12306	4009-4017	JJ	denotes	decidual
T12307	4033-4042	VBN	denotes	dissected
T12308	4028-4032	VBD	denotes	were
T12309	4043-4047	RB	denotes	away
T12310	4048-4052	IN	denotes	from
T12311	4053-4061	JJ	denotes	maternal
T12312	4062-4068	NN	denotes	tissue
T12313	4069-4072	CC	denotes	and
T12314	4073-4078	VBN	denotes	fixed
T12315	4079-4081	IN	denotes	in
T12316	4082-4083	CD	denotes	4
T12317	4083-4084	NN	denotes	%
T12318	4085-4101	NN	denotes	paraformaldehyde
T12319	4101-4102	HYPH	denotes	/
T12320	4102-4105	NN	denotes	PBS
T12321	4106-4115	RB	denotes	overnight
T12322	4116-4118	IN	denotes	at
T12323	4119-4120	CD	denotes	4
T12324	4121-4123	NN	denotes	°C
T12325	4123-4124	.	denotes	.
T12326	4124-4273	sentence	denotes	After embryos were washed thoroughly in PBS, they were dehydrated through an ethanol series and xylene, embedded in paraffin, and sectioned at 5 μm.
T12327	4125-4130	IN	denotes	After
T12328	4144-4150	VBN	denotes	washed
T12329	4131-4138	NNS	denotes	embryos
T12330	4139-4143	VBD	denotes	were
T12331	4180-4190	VBN	denotes	dehydrated
T12332	4151-4161	RB	denotes	thoroughly
T12333	4162-4164	IN	denotes	in
T12334	4165-4168	NN	denotes	PBS
T12335	4168-4170	,	denotes	,
T12336	4170-4174	PRP	denotes	they
T12337	4175-4179	VBD	denotes	were
T12338	4191-4198	IN	denotes	through
T12339	4199-4201	DT	denotes	an
T12340	4210-4216	NNS	denotes	series
T12341	4202-4209	NN	denotes	ethanol
T12342	4217-4220	CC	denotes	and
T12343	4221-4227	NN	denotes	xylene
T12344	4227-4229	,	denotes	,
T12345	4229-4237	VBN	denotes	embedded
T12346	4238-4240	IN	denotes	in
T12347	4241-4249	NN	denotes	paraffin
T12348	4249-4251	,	denotes	,
T12349	4251-4254	CC	denotes	and
T12350	4255-4264	VBN	denotes	sectioned
T12351	4265-4267	IN	denotes	at
T12352	4268-4269	CD	denotes	5
T12353	4270-4272	NNS	denotes	μm
T12354	4272-4273	.	denotes	.
T12355	4273-4464	sentence	denotes	Sections were processed for immunohistochemistry using the ABC Staining System (Santa Cruz Biotechnology, Santa Cruz, California, United States) according to the manufacturer's instructions.
T12356	4274-4282	NNS	denotes	Sections
T12357	4288-4297	VBN	denotes	processed
T12358	4283-4287	VBD	denotes	were
T12359	4298-4301	IN	denotes	for
T12360	4302-4322	NN	denotes	immunohistochemistry
T12361	4323-4328	VBG	denotes	using
T12362	4329-4332	DT	denotes	the
T12363	4346-4352	NNP	denotes	System
T12364	4333-4336	NNP	denotes	ABC
T12365	4337-4345	NNP	denotes	Staining
T12366	4353-4354	-LRB-	denotes	(
T12367	4365-4378	NNP	denotes	Biotechnology
T12368	4354-4359	NNP	denotes	Santa
T12369	4360-4364	NNP	denotes	Cruz
T12370	4378-4380	,	denotes	,
T12371	4380-4385	NNP	denotes	Santa
T12372	4386-4390	NNP	denotes	Cruz
T12373	4390-4392	,	denotes	,
T12374	4392-4402	NNP	denotes	California
T12375	4402-4404	,	denotes	,
T12376	4404-4410	NNP	denotes	United
T12377	4411-4417	NNP	denotes	States
T12378	4417-4418	-RRB-	denotes	)
T12379	4419-4428	VBG	denotes	according
T12380	4429-4431	IN	denotes	to
T12381	4432-4435	DT	denotes	the
T12382	4436-4448	NN	denotes	manufacturer
T12383	4451-4463	NNS	denotes	instructions
T12384	4448-4450	POS	denotes	's
T12385	4463-4464	.	denotes	.
T12386	4464-4762	sentence	denotes	Sections were stained with rabbit polyclonal antibodies against ATRX (H-300, Santa Cruz Biotechnology), Placental lactogen-I (AB1288, Chemicon International, Temecula, California, United States) and phospho (Ser10)-histone H3 (06–570, Upstate Biotechnology, Waltham, Massachusetts, United States).
T12387	4465-4473	NNS	denotes	Sections
T12388	4479-4486	VBN	denotes	stained
T12389	4474-4478	VBD	denotes	were
T12390	4487-4491	IN	denotes	with
T12391	4492-4498	NN	denotes	rabbit
T12392	4510-4520	NNS	denotes	antibodies
T12393	4499-4509	JJ	denotes	polyclonal
T12394	4521-4528	IN	denotes	against
T12395	4529-4533	NN	denotes	ATRX
T12396	4534-4535	-LRB-	denotes	(
T12397	4535-4536	NN	denotes	H
T12398	4536-4537	HYPH	denotes	-
T12399	4537-4540	CD	denotes	300
T12400	4540-4542	,	denotes	,
T12401	4542-4547	NNP	denotes	Santa
T12402	4548-4552	NNP	denotes	Cruz
T12403	4553-4566	NNP	denotes	Biotechnology
T12404	4566-4567	-RRB-	denotes	)
T12405	4567-4569	,	denotes	,
T12406	4569-4578	JJ	denotes	Placental
T12407	4588-4589	NN	denotes	I
T12408	4579-4587	NN	denotes	lactogen
T12409	4587-4588	HYPH	denotes	-
T12410	4590-4591	-LRB-	denotes	(
T12411	4591-4597	NN	denotes	AB1288
T12412	4597-4599	,	denotes	,
T12413	4599-4607	NNP	denotes	Chemicon
T12414	4608-4621	NNP	denotes	International
T12415	4621-4623	,	denotes	,
T12416	4623-4631	NNP	denotes	Temecula
T12417	4631-4633	,	denotes	,
T12418	4633-4643	NNP	denotes	California
T12419	4643-4645	,	denotes	,
T12420	4645-4651	NNP	denotes	United
T12421	4652-4658	NNP	denotes	States
T12422	4658-4659	-RRB-	denotes	)
T12423	4660-4663	CC	denotes	and
T12424	4664-4671	NN	denotes	phospho
T12425	4680-4687	NN	denotes	histone
T12426	4672-4673	-LRB-	denotes	(
T12427	4673-4678	NN	denotes	Ser10
T12428	4678-4679	-RRB-	denotes	)
T12429	4679-4680	HYPH	denotes	-
T12430	4688-4690	NN	denotes	H3
T12431	4691-4692	-LRB-	denotes	(
T12432	4695-4698	CD	denotes	570
T12433	4692-4694	CD	denotes	06
T12434	4694-4695	HYPH	denotes	–
T12435	4698-4700	,	denotes	,
T12436	4700-4707	NNP	denotes	Upstate
T12437	4708-4721	NNP	denotes	Biotechnology
T12438	4721-4723	,	denotes	,
T12439	4723-4730	NNP	denotes	Waltham
T12440	4730-4732	,	denotes	,
T12441	4732-4745	NNP	denotes	Massachusetts
T12442	4745-4747	,	denotes	,
T12443	4747-4753	NNP	denotes	United
T12444	4754-4760	NNP	denotes	States
T12445	4760-4761	-RRB-	denotes	)
T12446	4761-4762	.	denotes	.
T12447	4762-4831	sentence	denotes	Where appropriate, adjacent sections were stained with haematoxylin.
T12448	4763-4768	WRB	denotes	Where
T12449	4769-4780	JJ	denotes	appropriate
T12450	4805-4812	VBN	denotes	stained
T12451	4780-4782	,	denotes	,
T12452	4782-4790	JJ	denotes	adjacent
T12453	4791-4799	NNS	denotes	sections
T12454	4800-4804	VBD	denotes	were
T12455	4813-4817	IN	denotes	with
T12456	4818-4830	NN	denotes	haematoxylin
T12457	4830-4831	.	denotes	.
T12458	4831-4990	sentence	denotes	In some cases, adjacent sections were also analysed to detect apoptotic cells by TUNEL using the in situ cell death detection kit (Roche, Basel, Switzerland).
T12459	4832-4834	IN	denotes	In
T12460	4875-4883	VBN	denotes	analysed
T12461	4835-4839	DT	denotes	some
T12462	4840-4845	NNS	denotes	cases
T12463	4845-4847	,	denotes	,
T12464	4847-4855	JJ	denotes	adjacent
T12465	4856-4864	NNS	denotes	sections
T12466	4865-4869	VBD	denotes	were
T12467	4870-4874	RB	denotes	also
T12468	4884-4886	TO	denotes	to
T12469	4887-4893	VB	denotes	detect
T12470	4894-4903	JJ	denotes	apoptotic
T12471	4904-4909	NNS	denotes	cells
T12472	4910-4912	IN	denotes	by
T12473	4913-4918	NN	denotes	TUNEL
T12474	4919-4924	VBG	denotes	using
T12475	4925-4928	DT	denotes	the
T12476	4958-4961	NN	denotes	kit
T12477	4929-4931	FW	denotes	in
T12478	4932-4936	FW	denotes	situ
T12479	4937-4941	NN	denotes	cell
T12480	4942-4947	NN	denotes	death
T12481	4948-4957	NN	denotes	detection
T12482	4962-4963	-LRB-	denotes	(
T12483	4963-4968	NNP	denotes	Roche
T12484	4968-4970	,	denotes	,
T12485	4970-4975	NNP	denotes	Basel
T12486	4975-4977	,	denotes	,
T12487	4977-4988	NNP	denotes	Switzerland
T12488	4988-4989	-RRB-	denotes	)
T12489	4989-4990	.	denotes	.
T12490	4990-5168	sentence	denotes	After labelling, these slides were mounted in Vectashield containing DAPI (Vector Laboratories, Burlingame, California, United States) and visualised by fluorescence microscopy.
T12491	4991-4996	IN	denotes	After
T12492	5026-5033	VBN	denotes	mounted
T12493	4997-5006	NN	denotes	labelling
T12494	5006-5008	,	denotes	,
T12495	5008-5013	DT	denotes	these
T12496	5014-5020	NNS	denotes	slides
T12497	5021-5025	VBD	denotes	were
T12498	5034-5036	IN	denotes	in
T12499	5037-5048	NNP	denotes	Vectashield
T12500	5049-5059	VBG	denotes	containing
T12501	5060-5064	NN	denotes	DAPI
T12502	5065-5066	-LRB-	denotes	(
T12503	5073-5085	NNP	denotes	Laboratories
T12504	5066-5072	NNP	denotes	Vector
T12505	5085-5087	,	denotes	,
T12506	5087-5097	NNP	denotes	Burlingame
T12507	5097-5099	,	denotes	,
T12508	5099-5109	NNP	denotes	California
T12509	5109-5111	,	denotes	,
T12510	5111-5117	NNP	denotes	United
T12511	5118-5124	NNP	denotes	States
T12512	5124-5125	-RRB-	denotes	)
T12513	5126-5129	CC	denotes	and
T12514	5130-5140	VBN	denotes	visualised
T12515	5141-5143	IN	denotes	by
T12516	5144-5156	NN	denotes	fluorescence
T12517	5157-5167	NN	denotes	microscopy
T12518	5167-5168	.	denotes	.
T12519	5168-5499	sentence	denotes	Whole-mount in situ hybridisations were performed on 8.5 dpc embryos (dissected away from maternal and extraembryonic tissues) using a brachyury (T) riboprobe [16] and on bisected decidual implantation sites from which embryos (8.5 dpc) had been removed using a placental lactogen-1 (Pl-1) riboprobe (see Protocol S1 for details).
T12520	5169-5174	JJ	denotes	Whole
T12521	5175-5180	NN	denotes	mount
T12522	5174-5175	HYPH	denotes	-
T12523	5189-5203	NNS	denotes	hybridisations
T12524	5181-5183	FW	denotes	in
T12525	5184-5188	FW	denotes	situ
T12526	5209-5218	VBN	denotes	performed
T12527	5204-5208	VBD	denotes	were
T12528	5219-5221	IN	denotes	on
T12529	5222-5225	CD	denotes	8.5
T12530	5226-5229	NN	denotes	dpc
T12531	5230-5237	NNS	denotes	embryos
T12532	5238-5239	-LRB-	denotes	(
T12533	5239-5248	VBN	denotes	dissected
T12534	5249-5253	RB	denotes	away
T12535	5254-5258	IN	denotes	from
T12536	5259-5267	JJ	denotes	maternal
T12537	5287-5294	NNS	denotes	tissues
T12538	5268-5271	CC	denotes	and
T12539	5272-5286	JJ	denotes	extraembryonic
T12540	5294-5295	-RRB-	denotes	)
T12541	5296-5301	VBG	denotes	using
T12542	5302-5303	DT	denotes	a
T12543	5318-5327	NN	denotes	riboprobe
T12544	5304-5313	NN	denotes	brachyury
T12545	5314-5315	-LRB-	denotes	(
T12546	5315-5316	NN	denotes	T
T12547	5316-5317	-RRB-	denotes	)
T12548	5328-5329	-LRB-	denotes	[
T12549	5329-5331	CD	denotes	16
T12550	5331-5332	-RRB-	denotes	]
T12551	5333-5336	CC	denotes	and
T12552	5337-5339	IN	denotes	on
T12553	5340-5348	VBN	denotes	bisected
T12554	5371-5376	NNS	denotes	sites
T12555	5349-5357	JJ	denotes	decidual
T12556	5358-5370	NN	denotes	implantation
T12557	5377-5381	IN	denotes	from
T12558	5415-5422	VBN	denotes	removed
T12559	5382-5387	WDT	denotes	which
T12560	5388-5395	NNS	denotes	embryos
T12561	5396-5397	-LRB-	denotes	(
T12562	5401-5404	NN	denotes	dpc
T12563	5397-5400	CD	denotes	8.5
T12564	5404-5405	-RRB-	denotes	)
T12565	5406-5409	VBD	denotes	had
T12566	5410-5414	VBN	denotes	been
T12567	5423-5428	VBG	denotes	using
T12568	5429-5430	DT	denotes	a
T12569	5459-5468	NN	denotes	riboprobe
T12570	5431-5440	JJ	denotes	placental
T12571	5441-5449	NN	denotes	lactogen
T12572	5449-5450	HYPH	denotes	-
T12573	5450-5451	CD	denotes	1
T12574	5452-5453	-LRB-	denotes	(
T12575	5453-5455	NN	denotes	Pl
T12576	5455-5456	HYPH	denotes	-
T12577	5456-5457	CD	denotes	1
T12578	5457-5458	-RRB-	denotes	)
T12579	5469-5470	-LRB-	denotes	(
T12580	5470-5473	VB	denotes	see
T12581	5474-5482	NN	denotes	Protocol
T12582	5483-5485	NN	denotes	S1
T12583	5486-5489	IN	denotes	for
T12584	5490-5497	NNS	denotes	details
T12585	5497-5498	-RRB-	denotes	)
T12586	5498-5499	.	denotes	.
T12623	5501-5511	NN	denotes	Blastocyst
T12624	5512-5521	NN	denotes	outgrowth
T12625	5522-5530	NNS	denotes	cultures
T12626	5530-5531	.	denotes	.
T12627	5531-5725	sentence	denotes	Superovulated heterozygous female mice (Atrx WT/flox) were mated to homozygous GATA1-cre +/+ transgenic males, and blastocysts were flushed from uterine horns with M2 medium (Sigma) at 3.5 dpc.
T12628	5532-5545	VBN	denotes	Superovulated
T12629	5566-5570	NNS	denotes	mice
T12630	5546-5558	JJ	denotes	heterozygous
T12631	5559-5565	JJ	denotes	female
T12632	5591-5596	VBN	denotes	mated
T12633	5571-5572	-LRB-	denotes	(
T12634	5580-5584	NN	denotes	flox
T12635	5572-5579	NN	denotes	Atrx WT
T12636	5579-5580	HYPH	denotes	/
T12637	5584-5585	-RRB-	denotes	)
T12638	5586-5590	VBD	denotes	were
T12639	5597-5599	IN	denotes	to
T12640	5600-5610	JJ	denotes	homozygous
T12641	5636-5641	NNS	denotes	males
T12642	5611-5616	NN	denotes	GATA1
T12643	5617-5620	NN	denotes	cre
T12644	5616-5617	HYPH	denotes	-
T12645	5621-5622	SYM	denotes	+
T12646	5622-5623	HYPH	denotes	/
T12647	5623-5624	SYM	denotes	+
T12648	5625-5635	JJ	denotes	transgenic
T12649	5641-5643	,	denotes	,
T12650	5643-5646	CC	denotes	and
T12651	5647-5658	NNS	denotes	blastocysts
T12652	5664-5671	VBN	denotes	flushed
T12653	5659-5663	VBD	denotes	were
T12654	5672-5676	IN	denotes	from
T12655	5677-5684	JJ	denotes	uterine
T12656	5685-5690	NNS	denotes	horns
T12657	5691-5695	IN	denotes	with
T12658	5696-5698	NN	denotes	M2
T12659	5699-5705	NN	denotes	medium
T12660	5706-5707	-LRB-	denotes	(
T12661	5707-5712	NNP	denotes	Sigma
T12662	5712-5713	-RRB-	denotes	)
T12663	5714-5716	IN	denotes	at
T12664	5717-5720	CD	denotes	3.5
T12665	5721-5724	NNS	denotes	dpc
T12666	5724-5725	.	denotes	.
T12667	5725-5828	sentence	denotes	Individual blastocysts were cultured in multiwell tissue cultures plates as described previously [44].
T12668	5726-5736	JJ	denotes	Individual
T12669	5737-5748	NNS	denotes	blastocysts
T12670	5754-5762	VBN	denotes	cultured
T12671	5749-5753	VBD	denotes	were
T12672	5763-5765	IN	denotes	in
T12673	5766-5775	JJ	denotes	multiwell
T12674	5783-5791	NNS	denotes	cultures
T12675	5776-5782	NN	denotes	tissue
T12676	5792-5798	NNS	denotes	plates
T12677	5799-5801	IN	denotes	as
T12678	5802-5811	VBN	denotes	described
T12679	5812-5822	RB	denotes	previously
T12680	5823-5824	-LRB-	denotes	[
T12681	5824-5826	CD	denotes	44
T12682	5826-5827	-RRB-	denotes	]
T12683	5827-5828	.	denotes	.
T12684	5828-5911	sentence	denotes	Cultures were inspected and photographed daily and the extent of outgrowth scored.
T12685	5829-5837	NNS	denotes	Cultures
T12686	5843-5852	VBN	denotes	inspected
T12687	5838-5842	VBD	denotes	were
T12688	5853-5856	CC	denotes	and
T12689	5857-5869	VBN	denotes	photographed
T12690	5870-5875	RB	denotes	daily
T12691	5876-5879	CC	denotes	and
T12692	5880-5883	DT	denotes	the
T12693	5884-5890	NN	denotes	extent
T12694	5904-5910	VBN	denotes	scored
T12695	5891-5893	IN	denotes	of
T12696	5894-5903	NN	denotes	outgrowth
T12697	5910-5911	.	denotes	.
T12698	5911-5965	sentence	denotes	After 7 d, cultures were harvested and DNA extracted.
T12699	5912-5917	IN	denotes	After
T12700	5937-5946	VBN	denotes	harvested
T12701	5918-5919	CD	denotes	7
T12702	5920-5921	NNS	denotes	d
T12703	5921-5923	,	denotes	,
T12704	5923-5931	NNS	denotes	cultures
T12705	5932-5936	VBD	denotes	were
T12706	5947-5950	CC	denotes	and
T12707	5951-5954	NN	denotes	DNA
T12708	5955-5964	VBN	denotes	extracted
T12709	5964-5965	.	denotes	.
T12710	5965-6058	sentence	denotes	The Atrx genotype and sex of each culture was determined by PCR as described in Protocol S1.
T12711	5966-5969	DT	denotes	The
T12712	5975-5983	NN	denotes	genotype
T12713	5970-5974	NN	denotes	Atrx
T12714	6012-6022	VBN	denotes	determined
T12715	5984-5987	CC	denotes	and
T12716	5988-5991	NN	denotes	sex
T12717	5992-5994	IN	denotes	of
T12718	5995-5999	DT	denotes	each
T12719	6000-6007	NN	denotes	culture
T12720	6008-6011	VBD	denotes	was
T12721	6023-6025	IN	denotes	by
T12722	6026-6029	NN	denotes	PCR
T12723	6030-6032	IN	denotes	as
T12724	6033-6042	VBN	denotes	described
T12725	6043-6045	IN	denotes	in
T12726	6046-6054	NN	denotes	Protocol
T12727	6055-6057	NN	denotes	S1
T12728	6057-6058	.	denotes	.
R6941	T11190	T11189	prep	of,Generation
R6942	T11191	T11192	compound	ES,cells
R6943	T11192	T11190	pobj	cells,of
R6944	T11193	T11192	acl	bearing,cells
R6945	T11194	T11195	det	the,allele
R6946	T11195	T11193	dobj	allele,bearing
R6947	T11196	T11195	compound	Atrx flox,allele
R6948	T11197	T11189	punct	.,Generation
R6949	T11199	T11200	advmod	Briefly,places
R6950	T11201	T11200	punct	", ",places
R6951	T11202	T11203	det	the,vector
R6952	T11203	T11200	nsubj	vector,places
R6953	T11204	T11203	compound	targeting,vector
R6954	T11205	T11203	punct	(,vector
R6955	T11206	T11203	acl	shown,vector
R6956	T11207	T11206	prep	in,shown
R6957	T11208	T11209	compound	Figure,2A
R6958	T11209	T11207	pobj	2A,in
R6959	T11210	T11200	punct	),places
R6960	T11211	T11212	det	a,site
R6961	T11212	T11200	dobj	site,places
R6962	T11213	T11212	compound	loxP,site
R6963	T11214	T11200	prep	within,places
R6964	T11215	T11214	pobj	intron,within
R6965	T11216	T11215	nummod	18,intron
R6966	T11217	T11200	cc	and,places
R6967	T11218	T11219	det	a,cassette
R6968	T11219	T11200	conj	cassette,places
R6969	T11220	T11221	npadvmod	loxP,flanked
R6970	T11221	T11219	amod	flanked,cassette
R6971	T11222	T11221	punct	-,flanked
R6972	T11223	T11224	compound	MC1neopA,selection
R6973	T11224	T11219	compound	selection,cassette
R6974	T11225	T11219	prep	in,cassette
R6975	T11226	T11225	pobj	intron,in
R6976	T11227	T11226	nummod	17,intron
R6977	T11228	T11226	prep	of,intron
R6978	T11229	T11230	det	the,gene
R6979	T11230	T11228	pobj	gene,of
R6980	T11231	T11230	compound	Atrx,gene
R6981	T11232	T11200	punct	.,places
R6982	T11234	T11235	det	A,description
R6983	T11235	T11237	nsubjpass	description,provided
R6984	T11236	T11235	amod	detailed,description
R6985	T11238	T11235	prep	of,description
R6986	T11239	T11240	det	the,construct
R6987	T11240	T11238	pobj	construct,of
R6988	T11241	T11240	compound	targeting,construct
R6989	T11242	T11237	auxpass	is,provided
R6990	T11243	T11237	prep	in,provided
R6991	T11244	T11243	punct	[,in
R6992	T11245	T11243	pobj	20,in
R6993	T11246	T11237	punct	],provided
R6994	T11247	T11237	punct	.,provided
R6995	T11249	T11250	amod	Linearised,plasmid
R6996	T11250	T11251	nsubjpass	plasmid,electroporated
R6997	T11252	T11253	punct	(,μg
R6998	T11253	T11250	parataxis	μg,plasmid
R6999	T11254	T11253	nummod	150,μg
R7000	T11255	T11253	punct	),μg
R7001	T11256	T11251	auxpass	was,electroporated
R7002	T11257	T11251	prep	into,electroporated
R7003	T11258	T11259	quantmod	1,108
R7004	T11259	T11261	nummod	108,cells
R7005	T11260	T11259	punct	×,108
R7006	T11261	T11257	pobj	cells,into
R7007	T11262	T11261	compound	E14Tg2a,cells
R7008	T11263	T11261	compound	ES,cells
R7009	T11264	T11251	punct	", ",electroporated
R7010	T11265	T11251	cc	and,electroporated
R7011	T11266	T11267	nsubjpass	colonies,isolated
R7012	T11267	T11251	conj	isolated,electroporated
R7013	T11268	T11266	amod	resistant,colonies
R7014	T11269	T11268	prep	to,resistant
R7015	T11270	T11269	pobj	G418,to
R7016	T11271	T11270	cc	and,G418
R7017	T11272	T11270	conj	ganciclovir,G418
R7018	T11273	T11267	auxpass	were,isolated
R7019	T11274	T11267	punct	.,isolated
R7020	T11276	T11277	amod	Homologous,events
R7021	T11277	T11279	nsubjpass	events,identified
R7022	T11278	T11277	compound	targeting,events
R7023	T11280	T11279	auxpass	were,identified
R7024	T11281	T11279	prep	by,identified
R7025	T11282	T11283	compound	Southern,blot
R7026	T11283	T11281	pobj	blot,by
R7027	T11284	T11283	prep	of,blot
R7028	T11285	T11286	npadvmod	EcoRI,digested
R7029	T11286	T11288	amod	digested,DNA
R7030	T11287	T11286	punct	-,digested
R7031	T11288	T11284	pobj	DNA,of
R7032	T11289	T11283	cc	and,blot
R7033	T11290	T11283	conj	hybridisation,blot
R7034	T11291	T11290	prep	with,hybridisation
R7035	T11292	T11293	det	a,probe
R7036	T11293	T11291	pobj	probe,with
R7037	T11294	T11293	nummod	5,probe
R7038	T11295	T11294	punct	′,5
R7039	T11296	T11293	punct	(,probe
R7040	T11297	T11293	acl	generated,probe
R7041	T11298	T11297	prep	with,generated
R7042	T11299	T11300	compound	primers,PPS1.20
R7043	T11300	T11298	pobj	PPS1.20,with
R7044	T11301	T11300	cc	and,PPS1.20
R7045	T11302	T11300	conj	PPS1.27,PPS1.20
R7046	T11303	T11293	punct	),probe
R7047	T11304	T11293	cc	and,probe
R7048	T11305	T11306	det	a,probe
R7049	T11306	T11293	conj	probe,probe
R7050	T11307	T11306	nummod	3,probe
R7051	T11308	T11307	punct	′,3
R7052	T11309	T11306	punct	(,probe
R7053	T11310	T11311	det	a,fragment
R7054	T11311	T11306	appos	fragment,probe
R7055	T11312	T11313	nummod	0.9,kb
R7056	T11313	T11311	compound	kb,fragment
R7057	T11314	T11313	punct	-,kb
R7058	T11315	T11311	compound	HaeIII,fragment
R7059	T11316	T11279	punct	),identified
R7060	T11317	T11318	mark	as,shown
R7061	T11318	T11279	advcl	shown,identified
R7062	T11319	T11318	prep	in,shown
R7063	T11320	T11321	compound	Figure,2A
R7064	T11321	T11319	pobj	2A,in
R7065	T11322	T11321	cc	and,2A
R7066	T11323	T11321	conj	2B,2A
R7067	T11324	T11279	punct	.,identified
R7068	T11326	T11327	nsubj	DNA,digested
R7069	T11328	T11326	prep	from,DNA
R7070	T11329	T11330	advmod	correctly,targeted
R7071	T11330	T11331	amod	targeted,clones
R7072	T11331	T11328	pobj	clones,from
R7073	T11332	T11327	aux	was,digested
R7074	T11333	T11327	advmod	also,digested
R7075	T11334	T11327	prep	with,digested
R7076	T11335	T11334	pobj	SacI,with
R7077	T11336	T11327	cc	and,digested
R7078	T11337	T11327	conj	analysed,digested
R7079	T11338	T11337	prep	by,analysed
R7080	T11339	T11340	compound	Southern,blot
R7081	T11340	T11338	pobj	blot,by
R7082	T11341	T11340	cc	and,blot
R7083	T11342	T11340	conj	hybridisation,blot
R7084	T11343	T11342	prep	with,hybridisation
R7085	T11344	T11345	det	a,probe
R7086	T11345	T11343	pobj	probe,with
R7087	T11346	T11345	prep	from,probe
R7088	T11347	T11346	prep	within,from
R7089	T11348	T11347	pobj	intron,within
R7090	T11349	T11348	nummod	17,intron
R7091	T11350	T11345	punct	(,probe
R7092	T11351	T11352	det	a,product
R7093	T11352	T11345	appos	product,probe
R7094	T11353	T11352	compound	PCR,product
R7095	T11354	T11352	acl	generated,product
R7096	T11355	T11354	prep	with,generated
R7097	T11356	T11357	compound	primers,PPS1.15
R7098	T11357	T11355	pobj	PPS1.15,with
R7099	T11358	T11357	cc	and,PPS1.15
R7100	T11359	T11357	conj	Xnp46,PPS1.15
R7101	T11360	T11327	punct	),digested
R7102	T11361	T11362	aux	to,confirm
R7103	T11362	T11327	advcl	confirm,digested
R7104	T11363	T11364	mark	that,included
R7105	T11364	T11362	ccomp	included,confirm
R7106	T11365	T11366	det	the,site
R7107	T11366	T11364	nsubjpass	site,included
R7108	T11367	T11366	compound	loxP,site
R7109	T11368	T11366	prep	within,site
R7110	T11369	T11368	pobj	intron,within
R7111	T11370	T11369	nummod	18,intron
R7112	T11371	T11364	aux	had,included
R7113	T11372	T11364	auxpass	been,included
R7114	T11373	T11364	prep	within,included
R7115	T11374	T11375	det	the,region
R7116	T11375	T11373	pobj	region,within
R7117	T11376	T11375	amod	crossed,region
R7118	T11377	T11376	punct	-,crossed
R7119	T11378	T11376	prt	over,crossed
R7120	T11379	T11380	punct	(,2A
R7121	T11380	T11327	parataxis	2A,digested
R7122	T11381	T11380	compound	Figure,2A
R7123	T11382	T11380	cc	and,2A
R7124	T11383	T11380	conj	2C,2A
R7125	T11384	T11380	punct	),2A
R7126	T11385	T11327	punct	.,digested
R7127	T11387	T11388	nsubjpass	Sequences,shown
R7128	T11389	T11387	prep	of,Sequences
R7129	T11390	T11389	pobj	primers,of
R7130	T11391	T11388	auxpass	are,shown
R7131	T11392	T11388	prep	in,shown
R7132	T11393	T11394	compound	Table,S1
R7133	T11394	T11392	pobj	S1,in
R7134	T11395	T11388	punct	.,shown
R7135	T11569	T11570	compound	Cre,recombination
R7136	T11571	T11570	punct	-,recombination
R7137	T11572	T11570	cc	and,recombination
R7138	T11573	T11570	conj	characterisation,recombination
R7139	T11574	T11570	prep	of,recombination
R7140	T11575	T11576	amod	Atrxnull,cells
R7141	T11576	T11574	pobj	cells,of
R7142	T11577	T11576	compound	ES,cells
R7143	T11578	T11576	cc	and,cells
R7144	T11579	T11580	amod	embryoid,bodies
R7145	T11580	T11576	conj	bodies,cells
R7146	T11581	T11570	punct	.,recombination
R7147	T11583	T11584	compound	ES,cell
R7148	T11584	T11585	compound	cell,clones
R7149	T11585	T11586	nsubjpass	clones,transfected
R7150	T11587	T11585	acl	bearing,clones
R7151	T11588	T11589	det	the,allele
R7152	T11589	T11587	dobj	allele,bearing
R7153	T11590	T11589	compound	Atrx flox,allele
R7154	T11591	T11592	punct	(,cells
R7155	T11592	T11585	parataxis	cells,clones
R7156	T11593	T11594	quantmod	1,107
R7157	T11594	T11592	nummod	107,cells
R7158	T11595	T11594	punct	×,107
R7159	T11596	T11592	punct	),cells
R7160	T11597	T11586	auxpass	were,transfected
R7161	T11598	T11586	advmod	transiently,transfected
R7162	T11599	T11586	prep	with,transfected
R7163	T11600	T11601	nummod	50,μg
R7164	T11601	T11599	pobj	μg,with
R7165	T11602	T11601	prep	of,μg
R7166	T11603	T11604	amod	uncut,plasmid
R7167	T11604	T11602	pobj	plasmid,of
R7168	T11605	T11606	compound	Cre,expression
R7169	T11606	T11604	compound	expression,plasmid
R7170	T11607	T11604	punct	(,plasmid
R7171	T11608	T11609	compound	pCAGGS,IRESpuro
R7172	T11609	T11604	appos	IRESpuro,plasmid
R7173	T11610	T11609	punct	-,IRESpuro
R7174	T11611	T11609	compound	Cre,IRESpuro
R7175	T11612	T11609	punct	-,IRESpuro
R7176	T11613	T11586	punct	),transfected
R7177	T11614	T11615	punct	[,40
R7178	T11615	T11586	parataxis	40,transfected
R7179	T11616	T11615	punct	],40
R7180	T11617	T11586	punct	.,transfected
R7181	T11619	T11620	prep	Following,plated
R7182	T11621	T11619	pobj	transfection,Following
R7183	T11622	T11620	punct	", ",plated
R7184	T11623	T11620	nsubjpass	cells,plated
R7185	T11624	T11620	auxpass	were,plated
R7186	T11625	T11620	prep	at,plated
R7187	T11626	T11627	det	a,range
R7188	T11627	T11625	pobj	range,at
R7189	T11628	T11627	prep	of,range
R7190	T11629	T11630	amod	clonal,densities
R7191	T11630	T11628	pobj	densities,of
R7192	T11631	T11620	prep	in,plated
R7193	T11632	T11633	amod	complete,medium
R7194	T11633	T11631	pobj	medium,in
R7195	T11634	T11633	prep	without,medium
R7196	T11635	T11634	pobj	G418,without
R7197	T11636	T11620	punct	", ",plated
R7198	T11637	T11620	cc	and,plated
R7199	T11638	T11639	amod	isolated,subclones
R7200	T11639	T11640	nsubjpass	subclones,picked
R7201	T11640	T11620	conj	picked,plated
R7202	T11641	T11640	auxpass	were,picked
R7203	T11642	T11640	prep	after,picked
R7204	T11643	T11644	nummod	7,d
R7205	T11644	T11642	pobj	d,after
R7206	T11645	T11620	punct	.,plated
R7207	T11647	T11648	nsubjpass	Subclones,expanded
R7208	T11649	T11648	auxpass	were,expanded
R7209	T11650	T11648	cc	and,expanded
R7210	T11651	T11648	conj	analysed,expanded
R7211	T11652	T11651	prep	for,analysed
R7212	T11653	T11654	det	the,presence
R7213	T11654	T11652	pobj	presence,for
R7214	T11655	T11654	prep	of,presence
R7215	T11656	T11657	det	a,locus
R7216	T11657	T11655	pobj	locus,of
R7217	T11658	T11657	amod	recombinant,locus
R7218	T11659	T11660	advmod	initially,by
R7219	T11660	T11651	prep	by,analysed
R7220	T11661	T11660	pobj	PCR,by
R7221	T11662	T11660	punct	", ",by
R7222	T11663	T11664	aux	to,detect
R7223	T11664	T11660	advcl	detect,by
R7224	T11665	T11664	dobj	deletion,detect
R7225	T11666	T11665	prep	of,deletion
R7226	T11667	T11668	det	the,cassette
R7227	T11668	T11666	pobj	cassette,of
R7228	T11669	T11668	compound	MC1neopA,cassette
R7229	T11670	T11660	punct	", ",by
R7230	T11671	T11660	cc	and,by
R7231	T11672	T11673	advmod	then,by
R7232	T11673	T11660	conj	by,by
R7233	T11674	T11675	compound	Southern,blot
R7234	T11675	T11673	pobj	blot,by
R7235	T11676	T11675	cc	and,blot
R7236	T11677	T11675	conj	hybridisation,blot
R7237	T11678	T11675	prep	with,blot
R7238	T11679	T11680	det	the,probe
R7239	T11680	T11678	pobj	probe,with
R7240	T11681	T11680	nmod	intron,probe
R7241	T11682	T11681	nummod	17,intron
R7242	T11683	T11680	acl	described,probe
R7243	T11684	T11683	advmod	above,described
R7244	T11685	T11686	punct	(,2A
R7245	T11686	T11651	parataxis	2A,analysed
R7246	T11687	T11686	compound	Figure,2A
R7247	T11688	T11686	cc	and,2A
R7248	T11689	T11686	conj	2C,2A
R7249	T11690	T11686	punct	),2A
R7250	T11691	T11648	punct	.,expanded
R7251	T11693	T11694	compound	Northern,blots
R7252	T11694	T11695	nsubjpass	blots,carried
R7253	T11696	T11695	auxpass	were,carried
R7254	T11697	T11695	prt	out,carried
R7255	T11698	T11695	prep	according,carried
R7256	T11699	T11698	prep	to,according
R7257	T11700	T11701	amod	standard,techniques
R7258	T11701	T11699	pobj	techniques,to
R7259	T11702	T11695	advcl	using,carried
R7260	T11703	T11704	nummod	20,μg
R7261	T11704	T11702	dobj	μg,using
R7262	T11705	T11704	prep	of,μg
R7263	T11706	T11707	amod	total,RNA
R7264	T11707	T11705	pobj	RNA,of
R7265	T11708	T11707	acl	isolated,RNA
R7266	T11709	T11708	advcl	using,isolated
R7267	T11710	T11711	compound	TRI,Reagent
R7268	T11711	T11709	dobj	Reagent,using
R7269	T11712	T11713	punct	(,Aldrich
R7270	T11713	T11711	parataxis	Aldrich,Reagent
R7271	T11714	T11713	compound	Sigma,Aldrich
R7272	T11715	T11713	punct	-,Aldrich
R7273	T11716	T11713	punct	", ",Aldrich
R7274	T11717	T11718	compound	St.,Louis
R7275	T11718	T11713	npadvmod	Louis,Aldrich
R7276	T11719	T11713	punct	", ",Aldrich
R7277	T11720	T11713	npadvmod	Missouri,Aldrich
R7278	T11721	T11713	punct	", ",Aldrich
R7279	T11722	T11723	compound	United,States
R7280	T11723	T11713	npadvmod	States,Aldrich
R7281	T11724	T11713	punct	),Aldrich
R7282	T11725	T11695	punct	.,carried
R7283	T11727	T11728	det	The,blot
R7284	T11728	T11729	nsubjpass	blot,hybridised
R7285	T11730	T11729	auxpass	was,hybridised
R7286	T11731	T11729	prep	with,hybridised
R7287	T11732	T11733	det	a,probe
R7288	T11733	T11731	pobj	probe,with
R7289	T11734	T11733	prep	from,probe
R7290	T11735	T11734	prep	within,from
R7291	T11736	T11735	pobj	exon,within
R7292	T11737	T11736	nummod	10,exon
R7293	T11738	T11736	prep	of,exon
R7294	T11739	T11740	det	the,gene
R7295	T11740	T11738	pobj	gene,of
R7296	T11741	T11740	compound	Atrx,gene
R7297	T11742	T11733	punct	(,probe
R7298	T11743	T11733	acl	generated,probe
R7299	T11744	T11743	prep	with,generated
R7300	T11745	T11746	compound	primers,Mxnp4
R7301	T11746	T11744	pobj	Mxnp4,with
R7302	T11747	T11746	cc	and,Mxnp4
R7303	T11748	T11746	conj	Mxnp28,Mxnp4
R7304	T11749	T11750	punct	[,S1
R7305	T11750	T11729	parataxis	S1,hybridised
R7306	T11751	T11750	compound	Table,S1
R7307	T11752	T11750	punct	],S1
R7308	T11753	T11729	punct	),hybridised
R7309	T11754	T11729	punct	.,hybridised
R7310	T11756	T11757	mark	After,stripped
R7311	T11757	T11760	advcl	stripped,hybridised
R7312	T11758	T11757	nsubjpass	it,stripped
R7313	T11759	T11757	auxpass	was,stripped
R7314	T11761	T11760	punct	", ",hybridised
R7315	T11762	T11763	det	the,membrane
R7316	T11763	T11760	nsubjpass	membrane,hybridised
R7317	T11764	T11760	auxpass	was,hybridised
R7318	T11765	T11760	prep	with,hybridised
R7319	T11766	T11767	det	a,probe
R7320	T11767	T11765	pobj	probe,with
R7321	T11768	T11769	compound	β,actin
R7322	T11769	T11767	compound	actin,probe
R7323	T11770	T11769	punct	-,actin
R7324	T11771	T11767	compound	cDNA,probe
R7325	T11772	T11773	punct	(,Clontech
R7326	T11773	T11767	parataxis	Clontech,probe
R7327	T11774	T11773	punct	", ",Clontech
R7328	T11775	T11776	compound	Palo,Alto
R7329	T11776	T11773	npadvmod	Alto,Clontech
R7330	T11777	T11773	punct	", ",Clontech
R7331	T11778	T11773	npadvmod	California,Clontech
R7332	T11779	T11773	punct	", ",Clontech
R7333	T11780	T11781	compound	United,States
R7334	T11781	T11773	npadvmod	States,Clontech
R7335	T11782	T11773	punct	),Clontech
R7336	T11783	T11760	punct	.,hybridised
R7337	T11785	T11786	compound	Protein,extraction
R7338	T11786	T11787	nsubjpass	extraction,performed
R7339	T11788	T11786	cc	and,extraction
R7340	T11789	T11786	conj	detection,extraction
R7341	T11790	T11789	prep	of,detection
R7342	T11791	T11790	pobj	Atrx,of
R7343	T11792	T11789	prep	by,detection
R7344	T11793	T11794	compound	Western,blotting
R7345	T11794	T11792	pobj	blotting,by
R7346	T11795	T11787	auxpass	was,performed
R7347	T11796	T11797	mark	as,described
R7348	T11797	T11787	advcl	described,performed
R7349	T11798	T11797	advmod	previously,described
R7350	T11799	T11800	punct	[,4
R7351	T11800	T11797	parataxis	4,described
R7352	T11801	T11800	punct	],4
R7353	T11802	T11787	punct	", ",performed
R7354	T11803	T11787	advcl	using,performed
R7355	T11804	T11805	det	the,antibody
R7356	T11805	T11803	dobj	antibody,using
R7357	T11806	T11805	nmod	mouse,antibody
R7358	T11807	T11805	amod	monoclonal,antibody
R7359	T11808	T11805	amod	anti-ATRX,antibody
R7360	T11809	T11805	appos	23C,antibody
R7361	T11810	T11811	punct	[,15
R7362	T11811	T11787	parataxis	15,performed
R7363	T11812	T11811	punct	],15
R7364	T11813	T11787	punct	.,performed
R7365	T11815	T11816	nsubjpass	Analyses,described
R7366	T11817	T11815	prep	of,Analyses
R7367	T11818	T11819	compound	cell,cycle
R7368	T11819	T11817	pobj	cycle,of
R7369	T11820	T11819	cc	and,cycle
R7370	T11821	T11819	conj	apoptosis,cycle
R7371	T11822	T11816	auxpass	are,described
R7372	T11823	T11816	prep	in,described
R7373	T11824	T11825	compound	Protocol,S1
R7374	T11825	T11823	pobj	S1,in
R7375	T11826	T11816	punct	.,described
R7376	T11828	T11829	nsubjpass	Methylation,analysed
R7377	T11830	T11828	prep	of,Methylation
R7378	T11831	T11830	pobj	rDNA,of
R7379	T11832	T11829	auxpass	was,analysed
R7380	T11833	T11829	prep	in,analysed
R7381	T11834	T11833	pobj	DNA,in
R7382	T11835	T11834	prep	from,DNA
R7383	T11836	T11837	compound	ES,cell
R7384	T11837	T11838	compound	cell,clones
R7385	T11838	T11835	pobj	clones,from
R7386	T11839	T11835	cc	or,from
R7387	T11840	T11835	conj	from,from
R7388	T11841	T11842	amod	embryoid,bodies
R7389	T11842	T11840	pobj	bodies,from
R7390	T11843	T11842	acl	recovered,bodies
R7391	T11844	T11843	prep	after,recovered
R7392	T11845	T11846	nummod	7,d
R7393	T11846	T11844	pobj	d,after
R7394	T11847	T11846	prep	of,d
R7395	T11848	T11849	advmod	in,vitro
R7396	T11849	T11850	amod	vitro,differentiation
R7397	T11850	T11847	pobj	differentiation,of
R7398	T11851	T11852	mark	as,described
R7399	T11852	T11829	advcl	described,analysed
R7400	T11853	T11852	advmod	previously,described
R7401	T11854	T11855	punct	[,41
R7402	T11855	T11829	parataxis	41,analysed
R7403	T11856	T11855	punct	],41
R7404	T11857	T11829	punct	.,analysed
R7405	T11859	T11860	amod	Genomic,DNA
R7406	T11860	T11861	nsubjpass	DNA,digested
R7407	T11862	T11861	auxpass	was,digested
R7408	T11863	T11861	prep	with,digested
R7409	T11864	T11865	npadvmod	methylation,sensitive
R7410	T11865	T11867	amod	sensitive,enzymes
R7411	T11866	T11865	punct	-,sensitive
R7412	T11867	T11863	pobj	enzymes,with
R7413	T11868	T11867	compound	restriction,enzymes
R7414	T11869	T11870	mark	as,described
R7415	T11870	T11861	advcl	described,digested
R7416	T11871	T11861	cc	and,digested
R7417	T11872	T11861	conj	analysed,digested
R7418	T11873	T11872	prep	by,analysed
R7419	T11874	T11875	compound	Southern,blotting
R7420	T11875	T11873	pobj	blotting,by
R7421	T11876	T11861	punct	.,digested
R7422	T11878	T11879	det	The,probes
R7423	T11879	T11883	nsubjpass	probes,described
R7424	T11880	T11879	nmod	RIB3,probes
R7425	T11881	T11880	cc	and,RIB3
R7426	T11882	T11880	conj	RIB4,RIB3
R7427	T11884	T11879	punct	(,probes
R7428	T11885	T11886	dep	which,amplified
R7429	T11886	T11879	relcl	amplified,probes
R7430	T11887	T11886	auxpass	were,amplified
R7431	T11888	T11886	prep	from,amplified
R7432	T11889	T11890	amod	human,DNA
R7433	T11890	T11888	pobj	DNA,from
R7434	T11891	T11886	punct	", ",amplified
R7435	T11892	T11886	cc	but,amplified
R7436	T11893	T11886	conj	cross-react,amplified
R7437	T11894	T11893	prep	with,cross-react
R7438	T11895	T11896	det	the,repeat
R7439	T11896	T11894	pobj	repeat,with
R7440	T11897	T11898	compound	mouse,rDNA
R7441	T11898	T11896	compound	rDNA,repeat
R7442	T11899	T11879	punct	),probes
R7443	T11900	T11883	aux	have,described
R7444	T11901	T11883	auxpass	been,described
R7445	T11902	T11883	advmod	previously,described
R7446	T11903	T11904	punct	[,11
R7447	T11904	T11883	parataxis	11,described
R7448	T11905	T11904	punct	],11
R7449	T11906	T11883	punct	.,described
R7450	T11908	T11909	compound	Oligonucleotide,probes
R7451	T11909	T11910	nsubjpass	probes,described
R7452	T11911	T11912	aux	to,detect
R7453	T11912	T11909	advcl	detect,probes
R7454	T11913	T11914	nmod	Line,repeats
R7455	T11914	T11912	dobj	repeats,detect
R7456	T11915	T11913	nummod	1,Line
R7457	T11916	T11913	cc	and,Line
R7458	T11917	T11918	compound	Sine,B1
R7459	T11918	T11913	conj	B1,Line
R7460	T11919	T11910	aux	have,described
R7461	T11920	T11910	auxpass	been,described
R7462	T11921	T11910	advmod	previously,described
R7463	T11922	T11923	punct	[,42
R7464	T11923	T11910	parataxis	42,described
R7465	T11924	T11923	punct	],42
R7466	T11925	T11910	punct	.,described
R7467	T11927	T11928	det	The,probe
R7468	T11928	T11931	nsubj	probe,was
R7469	T11929	T11930	amod	minor,satellite
R7470	T11930	T11928	compound	satellite,probe
R7471	T11932	T11933	det	a,oligonucleotide
R7472	T11933	T11931	attr	oligonucleotide,was
R7473	T11934	T11935	nummod	27,mer
R7474	T11935	T11933	compound	mer,oligonucleotide
R7475	T11936	T11935	punct	-,mer
R7476	T11937	T11933	punct	(,oligonucleotide
R7477	T11938	T11933	appos	mCENT2,oligonucleotide
R7478	T11939	T11931	punct	),was
R7479	T11940	T11931	punct	.,was
R7480	T11942	T11943	det	The,probe
R7481	T11943	T11946	nsubj	probe,was
R7482	T11944	T11945	amod	major,satellite
R7483	T11945	T11943	compound	satellite,probe
R7484	T11947	T11948	det	a,oligonucleotide
R7485	T11948	T11946	attr	oligonucleotide,was
R7486	T11949	T11950	nummod	27,mer
R7487	T11950	T11948	compound	mer,oligonucleotide
R7488	T11951	T11950	punct	-,mer
R7489	T11952	T11948	punct	(,oligonucleotide
R7490	T11953	T11948	appos	DG27,oligonucleotide
R7491	T11954	T11946	punct	),was
R7492	T11955	T11946	punct	.,was
R7493	T11957	T11958	det	The,probe
R7494	T11958	T11960	nsubj	probe,was
R7495	T11959	T11958	compound	IAP,probe
R7496	T11961	T11962	det	an,product
R7497	T11962	T11960	attr	product,was
R7498	T11963	T11964	punct	~,400
R7499	T11964	T11965	nummod	400,bp
R7500	T11965	T11966	compound	bp,PCR
R7501	T11966	T11962	compound	PCR,product
R7502	T11967	T11962	punct	(,product
R7503	T11968	T11969	compound	primers,14A
R7504	T11969	T11962	appos	14A,product
R7505	T11970	T11969	cc	and,14A
R7506	T11971	T11969	conj	13K,14A
R7507	T11972	T11962	punct	),product
R7508	T11973	T11962	acl	amplified,product
R7509	T11974	T11973	prep	from,amplified
R7510	T11975	T11976	det	an,IAP
R7511	T11976	T11974	pobj	IAP,from
R7512	T11977	T11976	acl	inserted,IAP
R7513	T11978	T11977	prep	into,inserted
R7514	T11979	T11980	det	the,gene
R7515	T11980	T11978	pobj	gene,into
R7516	T11981	T11982	compound	mouse,agouti
R7517	T11982	T11980	compound	agouti,gene
R7518	T11983	T11984	punct	[,43
R7519	T11984	T11960	parataxis	43,was
R7520	T11985	T11984	punct	],43
R7521	T11986	T11960	cc	and,was
R7522	T11987	T11960	conj	was,was
R7523	T11988	T11989	det	a,gift
R7524	T11989	T11987	attr	gift,was
R7525	T11990	T11989	prep	from,gift
R7526	T11991	T11992	compound	Peter,Warnecke
R7527	T11992	T11990	pobj	Warnecke,from
R7528	T11993	T11992	cc	and,Warnecke
R7529	T11994	T11995	compound	Tim,Bestor
R7530	T11995	T11992	conj	Bestor,Warnecke
R7531	T11996	T11960	punct	.,was
R7532	T11998	T11999	det	The,product
R7533	T11999	T12001	nsubj	product,included
R7534	T12000	T11999	compound	PCR,product
R7535	T12002	T12003	det	the,LTR
R7536	T12003	T12001	dobj	LTR,included
R7537	T12004	T12003	amod	entire,LTR
R7538	T12005	T12003	nummod	5,LTR
R7539	T12006	T12005	punct	′,5
R7540	T12007	T12003	prep	of,LTR
R7541	T12008	T12009	det	the,IAP
R7542	T12009	T12007	pobj	IAP,of
R7543	T12010	T12001	punct	.,included
R7544	T12012	T12013	det	All,sequences
R7545	T12013	T12015	nsubjpass	sequences,shown
R7546	T12014	T12013	compound	oligonucleotide,sequences
R7547	T12016	T12015	auxpass	are,shown
R7548	T12017	T12015	prep	in,shown
R7549	T12018	T12019	compound	Table,S1
R7550	T12019	T12017	pobj	S1,in
R7551	T12020	T12015	punct	.,shown
R7552	T12081	T12080	prep	of,Generation
R7553	T12082	T12081	pobj	chimeras,of
R7554	T12083	T12082	punct	", ",chimeras
R7555	T12084	T12085	amod	floxed,mice
R7556	T12085	T12082	conj	mice,chimeras
R7557	T12086	T12085	punct	", ",mice
R7558	T12087	T12085	cc	and,mice
R7559	T12088	T12089	compound	mutant,mice
R7560	T12089	T12085	conj	mice,mice
R7561	T12090	T12080	punct	.,Generation
R7562	T12092	T12093	amod	Targeted,clones
R7563	T12093	T12097	nsubjpass	clones,injected
R7564	T12094	T12095	compound	Atrx flox,cell
R7565	T12095	T12093	compound	cell,clones
R7566	T12096	T12095	compound	ES,cell
R7567	T12098	T12097	auxpass	were,injected
R7568	T12099	T12097	prep	into,injected
R7569	T12100	T12101	nmod	C57BL,blastocysts
R7570	T12101	T12099	pobj	blastocysts,into
R7571	T12102	T12100	punct	/,C57BL
R7572	T12103	T12100	nummod	6,C57BL
R7573	T12104	T12097	cc	and,injected
R7574	T12105	T12097	conj	transferred,injected
R7575	T12106	T12105	prep	into,transferred
R7576	T12107	T12108	nummod	2.5,dpc
R7577	T12108	T12109	nmod	dpc,recipients
R7578	T12109	T12106	pobj	recipients,into
R7579	T12110	T12109	amod	pseudopregnant,recipients
R7580	T12111	T12097	prep	by,injected
R7581	T12112	T12113	amod	standard,techniques
R7582	T12113	T12111	pobj	techniques,by
R7583	T12114	T12097	punct	.,injected
R7584	T12116	T12117	amod	Resulting,chimeras
R7585	T12117	T12118	nsubjpass	chimeras,mated
R7586	T12119	T12118	auxpass	were,mated
R7587	T12120	T12118	prep	with,mated
R7588	T12121	T12120	pobj	C57BL,with
R7589	T12122	T12121	punct	/,C57BL
R7590	T12123	T12121	nummod	6,C57BL
R7591	T12124	T12125	aux	to,establish
R7592	T12125	T12118	advcl	establish,mated
R7593	T12126	T12127	compound	germline,transmission
R7594	T12127	T12125	dobj	transmission,establish
R7595	T12128	T12118	punct	.,mated
R7596	T12130	T12131	nsubjpass	Offspring,identified
R7597	T12132	T12130	prep	with,Offspring
R7598	T12133	T12134	det	the,allele
R7599	T12134	T12132	pobj	allele,with
R7600	T12135	T12134	compound	Atrx flox,allele
R7601	T12136	T12131	auxpass	were,identified
R7602	T12137	T12131	prep	by,identified
R7603	T12138	T12139	compound	Southern,blotting
R7604	T12139	T12137	pobj	blotting,by
R7605	T12140	T12139	prep	of,blotting
R7606	T12141	T12142	npadvmod	SacI,digested
R7607	T12142	T12144	amod	digested,DNA
R7608	T12143	T12142	punct	-,digested
R7609	T12144	T12140	pobj	DNA,of
R7610	T12145	T12144	compound	tail,DNA
R7611	T12146	T12131	advcl	using,identified
R7612	T12147	T12148	det	the,probe
R7613	T12148	T12146	dobj	probe,using
R7614	T12149	T12148	nmod	intron,probe
R7615	T12150	T12149	nummod	17,intron
R7616	T12151	T12152	mark	as,shown
R7617	T12152	T12146	advcl	shown,using
R7618	T12153	T12152	prep	in,shown
R7619	T12154	T12155	compound	Figure,2A
R7620	T12155	T12153	pobj	2A,in
R7621	T12156	T12155	cc	and,2A
R7622	T12157	T12155	conj	2C,2A
R7623	T12158	T12131	punct	.,identified
R7624	T12160	T12161	prep	For,crossed
R7625	T12162	T12163	compound	Cre,recombination
R7626	T12163	T12160	pobj	recombination,For
R7627	T12164	T12163	punct	-,recombination
R7628	T12165	T12161	punct	", ",crossed
R7629	T12166	T12167	compound	Atrx flox,mice
R7630	T12167	T12161	nsubjpass	mice,crossed
R7631	T12168	T12161	auxpass	were,crossed
R7632	T12169	T12161	prep	with,crossed
R7633	T12170	T12171	compound	GATA1,Cre
R7634	T12171	T12173	npadvmod	Cre,transgenic
R7635	T12172	T12171	punct	-,Cre
R7636	T12173	T12174	amod	transgenic,mice
R7637	T12174	T12169	pobj	mice,with
R7638	T12175	T12176	mark	as,described
R7639	T12176	T12161	advcl	described,crossed
R7640	T12177	T12176	prep	in,described
R7641	T12178	T12179	det	the,text
R7642	T12179	T12177	pobj	text,in
R7643	T12180	T12161	punct	.,crossed
R7644	T12182	T12183	amod	Recombinant,alleles
R7645	T12183	T12184	nsubjpass	alleles,detected
R7646	T12185	T12184	auxpass	were,detected
R7647	T12186	T12184	prep	by,detected
R7648	T12187	T12188	compound	Southern,blotting
R7649	T12188	T12186	pobj	blotting,by
R7650	T12189	T12190	mark	as,described
R7651	T12190	T12188	advcl	described,blotting
R7652	T12191	T12190	advmod	above,described
R7653	T12192	T12186	cc	or,by
R7654	T12193	T12186	conj	by,by
R7655	T12194	T12193	pobj	PCR,by
R7656	T12195	T12196	mark	as,described
R7657	T12196	T12184	advcl	described,detected
R7658	T12197	T12196	prep	in,described
R7659	T12198	T12199	compound	Protocol,S1
R7660	T12199	T12197	pobj	S1,in
R7661	T12200	T12184	punct	.,detected
R7662	T12293	T12292	punct	", ",Immunohistochemistry
R7663	T12294	T12295	nmod	in,situ
R7664	T12295	T12296	nmod	situ,hybridisation
R7665	T12296	T12292	conj	hybridisation,Immunohistochemistry
R7666	T12297	T12296	punct	", ",hybridisation
R7667	T12298	T12296	cc	and,hybridisation
R7668	T12299	T12300	compound	TUNEL,assay
R7669	T12300	T12296	conj	assay,hybridisation
R7670	T12301	T12300	punct	.,assay
R7671	T12303	T12304	nummod	7.5,dpc
R7672	T12304	T12305	nmod	dpc,swellings
R7673	T12305	T12307	nsubjpass	swellings,dissected
R7674	T12306	T12305	amod	decidual,swellings
R7675	T12308	T12307	auxpass	were,dissected
R7676	T12309	T12307	advmod	away,dissected
R7677	T12310	T12309	prep	from,away
R7678	T12311	T12312	amod	maternal,tissue
R7679	T12312	T12310	pobj	tissue,from
R7680	T12313	T12307	cc	and,dissected
R7681	T12314	T12307	conj	fixed,dissected
R7682	T12315	T12314	prep	in,fixed
R7683	T12316	T12317	nummod	4,%
R7684	T12317	T12318	compound	%,paraformaldehyde
R7685	T12318	T12315	pobj	paraformaldehyde,in
R7686	T12319	T12318	punct	/,paraformaldehyde
R7687	T12320	T12318	appos	PBS,paraformaldehyde
R7688	T12321	T12314	advmod	overnight,fixed
R7689	T12322	T12314	prep	at,fixed
R7690	T12323	T12324	nummod	4,°C
R7691	T12324	T12322	pobj	°C,at
R7692	T12325	T12307	punct	.,dissected
R7693	T12327	T12328	mark	After,washed
R7694	T12328	T12331	advcl	washed,dehydrated
R7695	T12329	T12328	nsubjpass	embryos,washed
R7696	T12330	T12328	auxpass	were,washed
R7697	T12332	T12328	advmod	thoroughly,washed
R7698	T12333	T12328	prep	in,washed
R7699	T12334	T12333	pobj	PBS,in
R7700	T12335	T12331	punct	", ",dehydrated
R7701	T12336	T12331	nsubjpass	they,dehydrated
R7702	T12337	T12331	auxpass	were,dehydrated
R7703	T12338	T12331	prep	through,dehydrated
R7704	T12339	T12340	det	an,series
R7705	T12340	T12338	pobj	series,through
R7706	T12341	T12340	compound	ethanol,series
R7707	T12342	T12340	cc	and,series
R7708	T12343	T12340	conj	xylene,series
R7709	T12344	T12331	punct	", ",dehydrated
R7710	T12345	T12331	conj	embedded,dehydrated
R7711	T12346	T12345	prep	in,embedded
R7712	T12347	T12346	pobj	paraffin,in
R7713	T12348	T12345	punct	", ",embedded
R7714	T12349	T12345	cc	and,embedded
R7715	T12350	T12345	conj	sectioned,embedded
R7716	T12351	T12350	prep	at,sectioned
R7717	T12352	T12353	nummod	5,μm
R7718	T12353	T12351	pobj	μm,at
R7719	T12354	T12331	punct	.,dehydrated
R7720	T12356	T12357	nsubjpass	Sections,processed
R7721	T12358	T12357	auxpass	were,processed
R7722	T12359	T12357	prep	for,processed
R7723	T12360	T12359	pobj	immunohistochemistry,for
R7724	T12361	T12357	advcl	using,processed
R7725	T12362	T12363	det	the,System
R7726	T12363	T12361	dobj	System,using
R7727	T12364	T12363	compound	ABC,System
R7728	T12365	T12363	compound	Staining,System
R7729	T12366	T12367	punct	(,Biotechnology
R7730	T12367	T12363	parataxis	Biotechnology,System
R7731	T12368	T12369	compound	Santa,Cruz
R7732	T12369	T12367	compound	Cruz,Biotechnology
R7733	T12370	T12367	punct	", ",Biotechnology
R7734	T12371	T12372	compound	Santa,Cruz
R7735	T12372	T12367	npadvmod	Cruz,Biotechnology
R7736	T12373	T12367	punct	", ",Biotechnology
R7737	T12374	T12367	npadvmod	California,Biotechnology
R7738	T12375	T12367	punct	", ",Biotechnology
R7739	T12376	T12377	compound	United,States
R7740	T12377	T12367	npadvmod	States,Biotechnology
R7741	T12378	T12367	punct	),Biotechnology
R7742	T12379	T12361	prep	according,using
R7743	T12380	T12379	prep	to,according
R7744	T12381	T12382	det	the,manufacturer
R7745	T12382	T12383	poss	manufacturer,instructions
R7746	T12383	T12380	pobj	instructions,to
R7747	T12384	T12382	case	's,manufacturer
R7748	T12385	T12357	punct	.,processed
R7749	T12387	T12388	nsubjpass	Sections,stained
R7750	T12389	T12388	auxpass	were,stained
R7751	T12390	T12388	prep	with,stained
R7752	T12391	T12392	nmod	rabbit,antibodies
R7753	T12392	T12390	pobj	antibodies,with
R7754	T12393	T12392	amod	polyclonal,antibodies
R7755	T12394	T12392	prep	against,antibodies
R7756	T12395	T12394	pobj	ATRX,against
R7757	T12396	T12397	punct	(,H
R7758	T12397	T12395	parataxis	H,ATRX
R7759	T12398	T12397	punct	-,H
R7760	T12399	T12397	nummod	300,H
R7761	T12400	T12397	punct	", ",H
R7762	T12401	T12402	compound	Santa,Cruz
R7763	T12402	T12403	compound	Cruz,Biotechnology
R7764	T12403	T12397	npadvmod	Biotechnology,H
R7765	T12404	T12397	punct	),H
R7766	T12405	T12395	punct	", ",ATRX
R7767	T12406	T12407	amod	Placental,I
R7768	T12407	T12395	conj	I,ATRX
R7769	T12408	T12407	compound	lactogen,I
R7770	T12409	T12407	punct	-,I
R7771	T12410	T12411	punct	(,AB1288
R7772	T12411	T12407	parataxis	AB1288,I
R7773	T12412	T12411	punct	", ",AB1288
R7774	T12413	T12414	compound	Chemicon,International
R7775	T12414	T12411	npadvmod	International,AB1288
R7776	T12415	T12411	punct	", ",AB1288
R7777	T12416	T12411	npadvmod	Temecula,AB1288
R7778	T12417	T12411	punct	", ",AB1288
R7779	T12418	T12411	npadvmod	California,AB1288
R7780	T12419	T12411	punct	", ",AB1288
R7781	T12420	T12421	compound	United,States
R7782	T12421	T12411	npadvmod	States,AB1288
R7783	T12422	T12411	punct	),AB1288
R7784	T12423	T12407	cc	and,I
R7785	T12424	T12425	nmod	phospho,histone
R7786	T12425	T12430	compound	histone,H3
R7787	T12426	T12427	punct	(,Ser10
R7788	T12427	T12425	parataxis	Ser10,histone
R7789	T12428	T12427	punct	),Ser10
R7790	T12429	T12425	punct	-,histone
R7791	T12430	T12407	conj	H3,I
R7792	T12431	T12432	punct	(,570
R7793	T12432	T12430	parataxis	570,H3
R7794	T12433	T12432	nummod	06,570
R7795	T12434	T12432	punct	–,570
R7796	T12435	T12432	punct	", ",570
R7797	T12436	T12437	compound	Upstate,Biotechnology
R7798	T12437	T12432	npadvmod	Biotechnology,570
R7799	T12438	T12432	punct	", ",570
R7800	T12439	T12432	npadvmod	Waltham,570
R7801	T12440	T12432	punct	", ",570
R7802	T12441	T12432	npadvmod	Massachusetts,570
R7803	T12442	T12432	punct	", ",570
R7804	T12443	T12444	compound	United,States
R7805	T12444	T12432	npadvmod	States,570
R7806	T12445	T12432	punct	),570
R7807	T12446	T12388	punct	.,stained
R7808	T12448	T12449	advmod	Where,appropriate
R7809	T12449	T12450	advcl	appropriate,stained
R7810	T12451	T12450	punct	", ",stained
R7811	T12452	T12453	amod	adjacent,sections
R7812	T12453	T12450	nsubjpass	sections,stained
R7813	T12454	T12450	auxpass	were,stained
R7814	T12455	T12450	prep	with,stained
R7815	T12456	T12455	pobj	haematoxylin,with
R7816	T12457	T12450	punct	.,stained
R7817	T12459	T12460	prep	In,analysed
R7818	T12461	T12462	det	some,cases
R7819	T12462	T12459	pobj	cases,In
R7820	T12463	T12460	punct	", ",analysed
R7821	T12464	T12465	amod	adjacent,sections
R7822	T12465	T12460	nsubjpass	sections,analysed
R7823	T12466	T12460	auxpass	were,analysed
R7824	T12467	T12460	advmod	also,analysed
R7825	T12468	T12469	aux	to,detect
R7826	T12469	T12460	advcl	detect,analysed
R7827	T12470	T12471	amod	apoptotic,cells
R7828	T12471	T12469	dobj	cells,detect
R7829	T12472	T12469	prep	by,detect
R7830	T12473	T12472	pobj	TUNEL,by
R7831	T12474	T12469	advcl	using,detect
R7832	T12475	T12476	det	the,kit
R7833	T12476	T12474	dobj	kit,using
R7834	T12477	T12478	advmod	in,situ
R7835	T12478	T12476	amod	situ,kit
R7836	T12479	T12480	compound	cell,death
R7837	T12480	T12481	compound	death,detection
R7838	T12481	T12476	compound	detection,kit
R7839	T12482	T12483	punct	(,Roche
R7840	T12483	T12474	parataxis	Roche,using
R7841	T12484	T12483	punct	", ",Roche
R7842	T12485	T12483	npadvmod	Basel,Roche
R7843	T12486	T12483	punct	", ",Roche
R7844	T12487	T12483	npadvmod	Switzerland,Roche
R7845	T12488	T12483	punct	),Roche
R7846	T12489	T12460	punct	.,analysed
R7847	T12491	T12492	prep	After,mounted
R7848	T12493	T12491	pobj	labelling,After
R7849	T12494	T12492	punct	", ",mounted
R7850	T12495	T12496	det	these,slides
R7851	T12496	T12492	nsubjpass	slides,mounted
R7852	T12497	T12492	auxpass	were,mounted
R7853	T12498	T12492	prep	in,mounted
R7854	T12499	T12498	pobj	Vectashield,in
R7855	T12500	T12499	acl	containing,Vectashield
R7856	T12501	T12500	dobj	DAPI,containing
R7857	T12502	T12503	punct	(,Laboratories
R7858	T12503	T12499	parataxis	Laboratories,Vectashield
R7859	T12504	T12503	compound	Vector,Laboratories
R7860	T12505	T12503	punct	", ",Laboratories
R7861	T12506	T12503	npadvmod	Burlingame,Laboratories
R7862	T12507	T12503	punct	", ",Laboratories
R7863	T12508	T12503	npadvmod	California,Laboratories
R7864	T12509	T12503	punct	", ",Laboratories
R7865	T12510	T12511	compound	United,States
R7866	T12511	T12503	npadvmod	States,Laboratories
R7867	T12512	T12503	punct	),Laboratories
R7868	T12513	T12492	cc	and,mounted
R7869	T12514	T12492	conj	visualised,mounted
R7870	T12515	T12514	prep	by,visualised
R7871	T12516	T12517	compound	fluorescence,microscopy
R7872	T12517	T12515	pobj	microscopy,by
R7873	T12518	T12492	punct	.,mounted
R7874	T12520	T12521	amod	Whole,mount
R7875	T12521	T12523	nmod	mount,hybridisations
R7876	T12522	T12521	punct	-,mount
R7877	T12523	T12526	nsubjpass	hybridisations,performed
R7878	T12524	T12525	advmod	in,situ
R7879	T12525	T12523	amod	situ,hybridisations
R7880	T12527	T12526	auxpass	were,performed
R7881	T12528	T12526	prep	on,performed
R7882	T12529	T12530	nummod	8.5,dpc
R7883	T12530	T12531	compound	dpc,embryos
R7884	T12531	T12528	pobj	embryos,on
R7885	T12532	T12531	punct	(,embryos
R7886	T12533	T12531	acl	dissected,embryos
R7887	T12534	T12533	advmod	away,dissected
R7888	T12535	T12534	prep	from,away
R7889	T12536	T12537	amod	maternal,tissues
R7890	T12537	T12535	pobj	tissues,from
R7891	T12538	T12536	cc	and,maternal
R7892	T12539	T12536	conj	extraembryonic,maternal
R7893	T12540	T12533	punct	),dissected
R7894	T12541	T12533	advcl	using,dissected
R7895	T12542	T12543	det	a,riboprobe
R7896	T12543	T12541	dobj	riboprobe,using
R7897	T12544	T12543	nmod	brachyury,riboprobe
R7898	T12545	T12544	punct	(,brachyury
R7899	T12546	T12544	appos	T,brachyury
R7900	T12547	T12543	punct	),riboprobe
R7901	T12548	T12549	punct	[,16
R7902	T12549	T12541	parataxis	16,using
R7903	T12550	T12549	punct	],16
R7904	T12551	T12528	cc	and,on
R7905	T12552	T12528	conj	on,on
R7906	T12553	T12554	amod	bisected,sites
R7907	T12554	T12552	pobj	sites,on
R7908	T12555	T12554	amod	decidual,sites
R7909	T12556	T12554	compound	implantation,sites
R7910	T12557	T12558	prep	from,removed
R7911	T12558	T12554	relcl	removed,sites
R7912	T12559	T12557	pobj	which,from
R7913	T12560	T12558	nsubjpass	embryos,removed
R7914	T12561	T12562	punct	(,dpc
R7915	T12562	T12560	parataxis	dpc,embryos
R7916	T12563	T12562	nummod	8.5,dpc
R7917	T12564	T12562	punct	),dpc
R7918	T12565	T12558	aux	had,removed
R7919	T12566	T12558	auxpass	been,removed
R7920	T12567	T12558	advcl	using,removed
R7921	T12568	T12569	det	a,riboprobe
R7922	T12569	T12567	dobj	riboprobe,using
R7923	T12570	T12571	amod	placental,lactogen
R7924	T12571	T12569	nmod	lactogen,riboprobe
R7925	T12572	T12571	punct	-,lactogen
R7926	T12573	T12571	nummod	1,lactogen
R7927	T12574	T12571	punct	(,lactogen
R7928	T12575	T12571	appos	Pl,lactogen
R7929	T12576	T12575	punct	-,Pl
R7930	T12577	T12575	nummod	1,Pl
R7931	T12578	T12569	punct	),riboprobe
R7932	T12579	T12580	punct	(,see
R7933	T12580	T12558	parataxis	see,removed
R7934	T12581	T12582	compound	Protocol,S1
R7935	T12582	T12580	dobj	S1,see
R7936	T12583	T12580	prep	for,see
R7937	T12584	T12583	pobj	details,for
R7938	T12585	T12580	punct	),see
R7939	T12586	T12526	punct	.,performed
R7940	T12623	T12624	compound	Blastocyst,outgrowth
R7941	T12624	T12625	compound	outgrowth,cultures
R7942	T12626	T12625	punct	.,cultures
R7943	T12628	T12629	amod	Superovulated,mice
R7944	T12629	T12632	nsubjpass	mice,mated
R7945	T12630	T12629	amod	heterozygous,mice
R7946	T12631	T12629	amod	female,mice
R7947	T12633	T12634	punct	(,flox
R7948	T12634	T12629	parataxis	flox,mice
R7949	T12635	T12634	compound	Atrx WT,flox
R7950	T12636	T12634	punct	/,flox
R7951	T12637	T12634	punct	),flox
R7952	T12638	T12632	auxpass	were,mated
R7953	T12639	T12632	prep	to,mated
R7954	T12640	T12641	amod	homozygous,males
R7955	T12641	T12639	pobj	males,to
R7956	T12642	T12643	nmod	GATA1,cre
R7957	T12643	T12641	nmod	cre,males
R7958	T12644	T12643	punct	-,cre
R7959	T12645	T12643	punct	+,cre
R7960	T12646	T12643	punct	/,cre
R7961	T12647	T12643	punct	+,cre
R7962	T12648	T12641	amod	transgenic,males
R7963	T12649	T12632	punct	", ",mated
R7964	T12650	T12632	cc	and,mated
R7965	T12651	T12652	nsubjpass	blastocysts,flushed
R7966	T12652	T12632	conj	flushed,mated
R7967	T12653	T12652	auxpass	were,flushed
R7968	T12654	T12652	prep	from,flushed
R7969	T12655	T12656	amod	uterine,horns
R7970	T12656	T12654	pobj	horns,from
R7971	T12657	T12652	prep	with,flushed
R7972	T12658	T12659	compound	M2,medium
R7973	T12659	T12657	pobj	medium,with
R7974	T12660	T12661	punct	(,Sigma
R7975	T12661	T12659	parataxis	Sigma,medium
R7976	T12662	T12661	punct	),Sigma
R7977	T12663	T12652	prep	at,flushed
R7978	T12664	T12665	nummod	3.5,dpc
R7979	T12665	T12663	pobj	dpc,at
R7980	T12666	T12652	punct	.,flushed
R7981	T12668	T12669	amod	Individual,blastocysts
R7982	T12669	T12670	nsubjpass	blastocysts,cultured
R7983	T12671	T12670	auxpass	were,cultured
R7984	T12672	T12670	prep	in,cultured
R7985	T12673	T12674	amod	multiwell,cultures
R7986	T12674	T12676	compound	cultures,plates
R7987	T12675	T12674	compound	tissue,cultures
R7988	T12676	T12672	pobj	plates,in
R7989	T12677	T12678	mark	as,described
R7990	T12678	T12670	advcl	described,cultured
R7991	T12679	T12678	advmod	previously,described
R7992	T12680	T12681	punct	[,44
R7993	T12681	T12670	parataxis	44,cultured
R7994	T12682	T12681	punct	],44
R7995	T12683	T12670	punct	.,cultured
R7996	T12685	T12686	nsubj	Cultures,inspected
R7997	T12687	T12686	aux	were,inspected
R7998	T12688	T12686	cc	and,inspected
R7999	T12689	T12686	conj	photographed,inspected
R8000	T12690	T12686	advmod	daily,inspected
R8001	T12691	T12686	cc	and,inspected
R8002	T12692	T12693	det	the,extent
R8003	T12693	T12694	nsubj	extent,scored
R8004	T12694	T12686	conj	scored,inspected
R8005	T12695	T12693	prep	of,extent
R8006	T12696	T12695	pobj	outgrowth,of
R8007	T12697	T12694	punct	.,scored
R8008	T12699	T12700	prep	After,harvested
R8009	T12701	T12702	nummod	7,d
R8010	T12702	T12699	pobj	d,After
R8011	T12703	T12700	punct	", ",harvested
R8012	T12704	T12700	nsubjpass	cultures,harvested
R8013	T12705	T12700	auxpass	were,harvested
R8014	T12706	T12700	cc	and,harvested
R8015	T12707	T12708	nsubj	DNA,extracted
R8016	T12708	T12700	conj	extracted,harvested
R8017	T12709	T12700	punct	.,harvested
R8018	T12711	T12712	det	The,genotype
R8019	T12712	T12714	nsubjpass	genotype,determined
R8020	T12713	T12712	compound	Atrx,genotype
R8021	T12715	T12712	cc	and,genotype
R8022	T12716	T12712	conj	sex,genotype
R8023	T12717	T12712	prep	of,genotype
R8024	T12718	T12719	det	each,culture
R8025	T12719	T12717	pobj	culture,of
R8026	T12720	T12714	auxpass	was,determined
R8027	T12721	T12714	prep	by,determined
R8028	T12722	T12721	pobj	PCR,by
R8029	T12723	T12724	mark	as,described
R8030	T12724	T12714	advcl	described,determined
R8031	T12725	T12724	prep	in,described
R8032	T12726	T12727	compound	Protocol,S1
R8033	T12727	T12725	pobj	S1,in
R8034	T12728	T12714	punct	.,determined

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id	Subject	Object	Predicate	Lexical cue
T11149	23-36	_FRAGMENT	denotes	Generation of
T11150	40-45	GO:0048468	denotes	cells
T11151	37-45	CL:0002322	denotes	ES cells
T11152	40-45	CL_GO_EXT:cell	denotes	cells
T11153	58-62	PR_EXT:000004503	denotes	Atrx
T11154	63-67	SO:0000359	denotes	flox
T11155	68-74	SO_EXT:0001023	denotes	allele
T11156	89-105	SO_EXT:0001644	denotes	targeting vector
T11157	136-145	SO_EXT:0000346	denotes	loxP site
T11158	153-159	SO_EXT:0000188	denotes	intron
T11159	169-181	SO:0000359	denotes	loxP-flanked
T11160	201-209	SO_EXT:0005853	denotes	cassette
T11161	213-219	SO_EXT:0000188	denotes	intron
T11162	230-234	PR_EXT:000004503	denotes	Atrx
T11163	235-239	SO_EXT:0000704	denotes	gene
T11164	281-290	SO_EXT:engineered_biological_sequence	denotes	construct
T11165	312-322	SO_EXT:sequence_linearization	denotes	Linearised
T11166	323-330	SO_EXT:0000155	denotes	plasmid
T11167	380-388	CL:0002322	denotes	ES cells
T11168	383-388	CL_GO_EXT:cell	denotes	cells
T11169	416-420	CHEBI:42768	denotes	G418
T11170	425-436	CHEBI:465284	denotes	ganciclovir
T11171	531-534	CHEBI_SO_EXT:DNA	denotes	DNA
T11172	539-552	GO:0097617	denotes	hybridisation
T11173	563-568	CHEBI_SO_EXT:molecular_probe	denotes	probe
T11174	585-592	SO_EXT:0000112	denotes	primers
T11175	623-628	CHEBI_SO_EXT:molecular_probe	denotes	probe
T11176	637-638	CHEBI_SO_EXT:base	denotes	b
T11177	686-689	CHEBI_SO_EXT:DNA	denotes	DNA
T11178	714-720	SO_EXT:sequence_cloned_entity	denotes	clones
T11179	783-796	GO:0097617	denotes	hybridisation
T11180	804-809	CHEBI_SO_EXT:molecular_probe	denotes	probe
T11181	822-828	SO_EXT:0000188	denotes	intron
T11182	835-846	SO_EXT:0000006	denotes	PCR product
T11183	862-869	SO_EXT:0000112	denotes	primers
T11184	909-918	SO_EXT:0000346	denotes	loxP site
T11185	926-932	SO_EXT:0000188	denotes	intron
T11186	965-977	GO:0035825	denotes	crossed-over
T11187	1005-1014	SO_EXT:biological_sequence	denotes	Sequences
T11188	1018-1025	SO_EXT:0000112	denotes	primers
T11483	1054-1067	GO_SO_EXT:sequence_rearrangement_process	denotes	recombination
T11484	1092-1096	PR_EXT:000004503	denotes	Atrx
T11485	1096-1100	SO_EXT:sequence_nullness	denotes	null
T11486	1101-1109	CL:0002322	denotes	ES cells
T11487	1104-1109	CL_GO_EXT:cell	denotes	cells
T11488	1114-1129	UBERON:0014374	denotes	embryoid bodies
T11489	1131-1138	CL:0002322	denotes	ES cell
T11490	1134-1138	CL_GO_EXT:cell	denotes	cell
T11491	1139-1145	SO_EXT:sequence_cloned_entity	denotes	clones
T11492	1158-1162	PR_EXT:000004503	denotes	Atrx
T11493	1163-1167	SO:0000359	denotes	flox
T11494	1168-1174	SO_EXT:0001023	denotes	allele
T11495	1184-1189	CL_GO_EXT:cell	denotes	cells
T11496	1208-1219	GO:0009294	denotes	transfected
T11497	1236-1239	MOP:0000780	denotes	cut
T11498	1244-1254	GO:0010467	denotes	expression
T11499	1255-1262	SO_EXT:0000155	denotes	plasmid
T11500	1275-1279	SO_EXT:0000243	denotes	IRES
T11501	1301-1313	GO:0009294	denotes	transfection
T11502	1315-1320	CL_GO_EXT:cell	denotes	cells
T11503	1347-1353	SO_EXT:sequence_cloned_entity	denotes	clonal
T11504	1391-1395	CHEBI:42768	denotes	G418
T11505	1502-1513	GO_SO_EXT:sequence_rearrangement_process	denotes	recombinant
T11506	1548-1556	SO_EXT:sequence_deletion_entity_or_process	denotes	deletion
T11507	1573-1581	SO_EXT:0005853	denotes	cassette
T11508	1613-1626	GO:0097617	denotes	hybridisation
T11509	1636-1642	SO_EXT:0000188	denotes	intron
T11510	1646-1651	CHEBI_SO_EXT:molecular_probe	denotes	probe
T11511	1774-1777	CHEBI_SO_EXT:RNA	denotes	RNA
T11512	1797-1804	CHEBI_EXT:33893	denotes	Reagent
T11513	1871-1881	GO:0097617	denotes	hybridised
T11514	1889-1894	CHEBI_SO_EXT:molecular_probe	denotes	probe
T11515	1907-1911	SO_EXT:0000147	denotes	exon
T11516	1922-1926	PR_EXT:000004503	denotes	Atrx
T11517	1927-1931	SO_EXT:0000704	denotes	gene
T11518	1948-1955	SO_EXT:0000112	denotes	primers
T11519	2026-2036	GO:0097617	denotes	hybridised
T11520	2044-2051	PR_EXT:000003676	denotes	β-actin
T11521	2052-2056	SO_EXT:cDNA	denotes	cDNA
T11522	2057-2062	CHEBI_SO_EXT:molecular_probe	denotes	probe
T11523	2113-2120	CHEBI_PR_EXT:protein	denotes	Protein
T11524	2149-2153	PR_EXT:000004503	denotes	Atrx
T11525	2227-2232	NCBITaxon:10088	denotes	mouse
T11526	2249-2253	PR_EXT:000004503	denotes	ATRX
T11527	2254-2262	GO:0042571	denotes	antibody
T11528	2285-2289	CL_GO_EXT:cell	denotes	cell
T11529	2285-2295	GO:0007049	denotes	cell cycle
T11530	2300-2309	GO:0006915	denotes	apoptosis
T11531	2340-2351	GO_MOP_EXT:methylation	denotes	Methylation
T11532	2376-2379	CHEBI_SO_EXT:DNA	denotes	DNA
T11533	2385-2392	CL:0002322	denotes	ES cell
T11534	2388-2392	CL_GO_EXT:cell	denotes	cell
T11535	2393-2399	SO_EXT:sequence_cloned_entity	denotes	clones
T11536	2408-2423	UBERON:0014374	denotes	embryoid bodies
T11537	2456-2471	GO_RO_EXT:developmental_differentiation_process	denotes	differentiation
T11538	2502-2513	SO_EXT:genomic_DNA	denotes	Genomic DNA
T11539	2510-2513	CHEBI_SO_EXT:DNA	denotes	DNA
T11540	2532-2543	GO_MOP_EXT:methylation	denotes	methylation
T11541	2554-2573	GO_EXT:0015666	denotes	restriction enzymes
T11542	2566-2573	CHEBI_GO_SO_EXT:enzyme	denotes	enzymes
T11543	2640-2646	CHEBI_SO_EXT:molecular_probe	denotes	probes
T11544	2674-2679	NCBITaxon:9606	denotes	human
T11545	2680-2683	CHEBI_SO_EXT:DNA	denotes	DNA
T11546	2710-2715	NCBITaxon:10088	denotes	mouse
T11547	2721-2727	SO_EXT:sequence_repeat_unit_or_region	denotes	repeat
T11548	2766-2781	CHEBI_SO_EXT:oligonucleotide	denotes	Oligonucleotide
T11549	2782-2788	CHEBI_SO_EXT:molecular_probe	denotes	probes
T11550	2818-2825	SO_EXT:sequence_repeat_unit_or_region	denotes	repeats
T11551	2873-2882	SO_EXT:satellite_unit_or_region	denotes	satellite
T11552	2883-2888	CHEBI_SO_EXT:molecular_probe	denotes	probe
T11553	2902-2917	CHEBI_SO_EXT:oligonucleotide	denotes	oligonucleotide
T11554	2938-2947	SO_EXT:satellite_unit_or_region	denotes	satellite
T11555	2948-2953	CHEBI_SO_EXT:molecular_probe	denotes	probe
T11556	2967-2982	CHEBI_SO_EXT:oligonucleotide	denotes	oligonucleotide
T11557	2999-3004	CHEBI_SO_EXT:molecular_probe	denotes	probe
T11558	3017-3019	SO_EXT:0000028	denotes	bp
T11559	3020-3031	SO_EXT:0000006	denotes	PCR product
T11560	3033-3040	SO_EXT:0000112	denotes	primers
T11561	3076-3084	SO_EXT:sequence_insertion_process	denotes	inserted
T11562	3094-3099	NCBITaxon:10088	denotes	mouse
T11563	3100-3106	PR_EXT:000004372	denotes	agouti
T11564	3107-3111	SO_EXT:0000704	denotes	gene
T11565	3172-3183	SO_EXT:0000006	denotes	PCR product
T11566	3204-3210	SO_EXT:5_prime_LTR_unit_or_region	denotes	5′ LTR
T11567	3227-3242	CHEBI_SO_EXT:oligonucleotide	denotes	oligonucleotide
T11568	3243-3252	SO_EXT:biological_sequence	denotes	sequences
T12052	3301-3307	SO:0000359	denotes	floxed
T12053	3308-3312	NCBITaxon:10088	denotes	mice
T12054	3318-3324	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T12055	3325-3329	NCBITaxon:10088	denotes	mice
T12056	3340-3344	PR_EXT:000004503	denotes	Atrx
T12057	3345-3349	SO:0000359	denotes	flox
T12058	3350-3357	CL:0002322	denotes	ES cell
T12059	3353-3357	CL_GO_EXT:cell	denotes	cell
T12060	3358-3364	SO_EXT:sequence_cloned_entity	denotes	clones
T12061	3392-3403	UBERON:0000358	denotes	blastocysts
T12062	3408-3419	GO_EXT:biological_movement_or_translocation_process	denotes	transferred
T12063	3507-3512	GO:0007618	denotes	mated
T12064	3581-3585	PR_EXT:000004503	denotes	Atrx
T12065	3586-3590	SO:0000359	denotes	flox
T12066	3591-3597	SO_EXT:0001023	denotes	allele
T12067	3652-3656	UBERON:0002415	denotes	tail
T12068	3657-3660	CHEBI_SO_EXT:DNA	denotes	DNA
T12069	3671-3677	SO_EXT:0000188	denotes	intron
T12070	3681-3686	CHEBI_SO_EXT:molecular_probe	denotes	probe
T12071	3725-3738	GO_SO_EXT:sequence_rearrangement_process	denotes	recombination
T12072	3740-3744	PR_EXT:000004503	denotes	Atrx
T12073	3745-3749	SO:0000359	denotes	flox
T12074	3750-3754	NCBITaxon:10088	denotes	mice
T12075	3773-3778	PR_EXT:000007857	denotes	GATA1
T12076	3783-3793	SO_EXT:transgenic_entity	denotes	transgenic
T12077	3794-3798	NCBITaxon:10088	denotes	mice
T12078	3825-3836	GO_SO_EXT:sequence_rearrangement_process	denotes	Recombinant
T12079	3837-3844	SO_EXT:0001023	denotes	alleles
T12252	3969-3982	GO:0097617	denotes	hybridisation
T12253	4009-4017	UBERON:0002450	denotes	decidual
T12254	4062-4068	UBERON:0000479	denotes	tissue
T12255	4085-4101	CHEBI_EXT:paraformaldehye	denotes	paraformaldehyde
T12256	4131-4138	UBERON:0000922	denotes	embryos
T12257	4202-4209	CHEBI:16236	denotes	ethanol
T12258	4221-4227	CHEBI:27338	denotes	xylene
T12259	4492-4498	NCBITaxon:9986	denotes	rabbit
T12260	4510-4520	GO:0042571	denotes	antibodies
T12261	4529-4533	PR_EXT:000004503	denotes	ATRX
T12262	4569-4578	UBERON:0001987	denotes	Placental
T12263	4569-4587	CHEBI:81588	denotes	Placental lactogen
T12264	4569-4589	PR_EXT:000013543	denotes	Placental lactogen-I
T12265	4664-4671	CHEBI:32958	denotes	phospho
T12266	4680-4687	CHEBI:15358	denotes	histone
T12267	4680-4690	PR_EXT:000027594	denotes	histone H3
T12268	4818-4830	CHEBI:51686	denotes	haematoxylin
T12269	4894-4903	GO_PATO_EXT:apoptotic_process_or_quality	denotes	apoptotic
T12270	4894-4909	CL:0000445	denotes	apoptotic cells
T12271	4904-4909	CL_GO_EXT:cell	denotes	cells
T12272	4937-4941	CL_GO_EXT:cell	denotes	cell
T12273	4937-4947	GO:0008219	denotes	cell death
T12274	4997-5006	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	labelling
T12275	5060-5064	CHEBI:51231	denotes	DAPI
T12276	5066-5072	SO_EXT:0000440	denotes	Vector
T12277	5189-5203	GO:0097617	denotes	hybridisations
T12278	5230-5237	UBERON:0000922	denotes	embryos
T12279	5272-5294	UBERON:0005292	denotes	extraembryonic tissues
T12280	5304-5313	PR_EXT:000016001	denotes	brachyury
T12281	5315-5316	PR_EXT:000016001	denotes	T
T12282	5318-5327	CHEBI_SO_EXT:riboprobe	denotes	riboprobe
T12283	5349-5357	UBERON:0002450	denotes	decidual
T12284	5358-5370	GO:0007566	denotes	implantation
T12285	5388-5395	UBERON:0000922	denotes	embryos
T12286	5431-5440	UBERON:0001987	denotes	placental
T12287	5431-5449	CHEBI:81588	denotes	placental lactogen
T12288	5431-5451	PR_EXT:000013543	denotes	placental lactogen-1
T12289	5453-5455	CHEBI:81588	denotes	Pl
T12290	5453-5457	PR_EXT:000013543	denotes	Pl-1
T12291	5459-5468	CHEBI_SO_EXT:riboprobe	denotes	riboprobe
T12609	5580-5584	SO:0000359	denotes	flox
T12610	5591-5596	GO:0007618	denotes	mated
T12611	5611-5616	PR_EXT:000007857	denotes	GATA1
T12612	5621-5622	SO_EXT:normal_or_wild_type_or_present	denotes	+
T12613	5623-5624	SO_EXT:normal_or_wild_type_or_present	denotes	+
T12614	5625-5635	SO_EXT:transgenic_entity	denotes	transgenic
T12615	5636-5641	PATO_UBERON_EXT:male_or_bearer_of_maleness	denotes	males
T12616	5647-5658	UBERON:0000358	denotes	blastocysts
T12617	5677-5690	UBERON:0002247	denotes	uterine horns
T12618	5737-5748	UBERON:0000358	denotes	blastocysts
T12619	5776-5782	UBERON:0000479	denotes	tissue
T12620	5951-5954	CHEBI_SO_EXT:DNA	denotes	DNA
T12621	5970-5974	PR_EXT:000004503	denotes	Atrx
T12622	5975-5983	SO_EXT:genotype_or_entity_with_genotype	denotes	genotype
T12603	5501-5511	UBERON:0000358	denotes	Blastocyst
T12604	5537-5545	GO:0030728	denotes	ovulated
T12605	5559-5565	PATO_UBERON_EXT:female_or_bearer_of_femaleness	denotes	female
T12606	5566-5570	NCBITaxon:10088	denotes	mice
T12607	5572-5576	PR_EXT:000004503	denotes	Atrx
T12608	5577-5579	SO_EXT:wild_type_entity_or_quality	denotes	WT
R6940	T11150	T11149	_lexicallyChainedTo	cells,Generation of

2_test

Id	Subject	Object	Predicate	Lexical cue
16628246-15668733-85799784	307-309	15668733	denotes	20
16628246-12085230-85799785	1286-1288	12085230	denotes	40
16628246-14729260-85799786	2213-2214	14729260	denotes	4
16628246-10570185-85799787	2268-2270	10570185	denotes	15
16628246-8417345-85799788	2497-2499	8417345	denotes	41
16628246-10742099-85799789	2761-2763	10742099	denotes	11
16628246-11711429-85799790	2858-2860	11711429	denotes	42
16628246-7926745-85799791	3113-3115	7926745	denotes	43
16628246-1689462-85799792	5329-5331	1689462	denotes	16
16628246-10859163-85799793	5824-5826	10859163	denotes	44
T64563	307-309	15668733	denotes	20
T83163	1286-1288	12085230	denotes	40
T14123	2213-2214	14729260	denotes	4
T85420	2268-2270	10570185	denotes	15
T26504	2497-2499	8417345	denotes	41
T27595	2761-2763	10742099	denotes	11
T53645	2858-2860	11711429	denotes	42
T82024	3113-3115	7926745	denotes	43
T74395	5329-5331	1689462	denotes	16
T86047	5824-5826	10859163	denotes	44

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id	Subject	Object	Predicate	Lexical cue
T11121	23-36	_FRAGMENT	denotes	Generation of
T11122	40-45	GO:0048468	denotes	cells
T11123	37-45	CL:0002322	denotes	ES cells
T11124	58-62	PR:000004503	denotes	Atrx
T11125	63-67	SO:0000359	denotes	flox
T11126	68-74	SO:0001023	denotes	allele
T11127	89-105	SO:0001644	denotes	targeting vector
T11128	136-145	SO:0000346	denotes	loxP site
T11129	153-159	SO:0000188	denotes	intron
T11130	169-181	SO:0000359	denotes	loxP-flanked
T11131	201-209	SO:0005853	denotes	cassette
T11132	213-219	SO:0000188	denotes	intron
T11133	230-234	PR:000004503	denotes	Atrx
T11134	235-239	SO:0000704	denotes	gene
T11135	323-330	SO:0000155	denotes	plasmid
T11136	380-388	CL:0002322	denotes	ES cells
T11137	416-420	CHEBI:42768	denotes	G418
T11138	425-436	CHEBI:465284	denotes	ganciclovir
T11139	539-552	GO:0097617	denotes	hybridisation
T11140	585-592	SO:0000112	denotes	primers
T11141	783-796	GO:0097617	denotes	hybridisation
T11142	822-828	SO:0000188	denotes	intron
T11143	835-846	SO:0000006	denotes	PCR product
T11144	862-869	SO:0000112	denotes	primers
T11145	909-918	SO:0000346	denotes	loxP site
T11146	926-932	SO:0000188	denotes	intron
T11147	965-977	GO:0035825	denotes	crossed-over
T11148	1018-1025	SO:0000112	denotes	primers
T11440	1092-1096	PR:000004503	denotes	Atrx
T11441	1101-1109	CL:0002322	denotes	ES cells
T11442	1114-1129	UBERON:0014374	denotes	embryoid bodies
T11443	1131-1138	CL:0002322	denotes	ES cell
T11444	1158-1162	PR:000004503	denotes	Atrx
T11445	1163-1167	SO:0000359	denotes	flox
T11446	1168-1174	SO:0001023	denotes	allele
T11447	1208-1219	GO:0009294	denotes	transfected
T11448	1236-1239	MOP:0000780	denotes	cut
T11449	1244-1254	GO:0010467	denotes	expression
T11450	1255-1262	SO:0000155	denotes	plasmid
T11451	1275-1279	SO:0000243	denotes	IRES
T11452	1301-1313	GO:0009294	denotes	transfection
T11453	1391-1395	CHEBI:42768	denotes	G418
T11454	1573-1581	SO:0005853	denotes	cassette
T11455	1613-1626	GO:0097617	denotes	hybridisation
T11456	1636-1642	SO:0000188	denotes	intron
T11457	1797-1804	CHEBI:33893	denotes	Reagent
T11458	1871-1881	GO:0097617	denotes	hybridised
T11459	1907-1911	SO:0000147	denotes	exon
T11460	1922-1926	PR:000004503	denotes	Atrx
T11461	1927-1931	SO:0000704	denotes	gene
T11462	1948-1955	SO:0000112	denotes	primers
T11463	2026-2036	GO:0097617	denotes	hybridised
T11464	2044-2051	PR:000003676	denotes	β-actin
T11465	2149-2153	PR:000004503	denotes	Atrx
T11466	2227-2232	NCBITaxon:10088	denotes	mouse
T11467	2249-2253	PR:000004503	denotes	ATRX
T11468	2254-2262	GO:0042571	denotes	antibody
T11469	2285-2295	GO:0007049	denotes	cell cycle
T11470	2300-2309	GO:0006915	denotes	apoptosis
T11471	2385-2392	CL:0002322	denotes	ES cell
T11472	2408-2423	UBERON:0014374	denotes	embryoid bodies
T11473	2502-2509	SO:0001026	denotes	Genomic
T11474	2674-2679	NCBITaxon:9606	denotes	human
T11475	2710-2715	NCBITaxon:10088	denotes	mouse
T11476	3017-3019	SO:0000028	denotes	bp
T11477	3020-3031	SO:0000006	denotes	PCR product
T11478	3033-3040	SO:0000112	denotes	primers
T11479	3094-3099	NCBITaxon:10088	denotes	mouse
T11480	3100-3106	PR:000004372	denotes	agouti
T11481	3107-3111	SO:0000704	denotes	gene
T11482	3172-3183	SO:0000006	denotes	PCR product
T12033	3301-3307	SO:0000359	denotes	floxed
T12034	3308-3312	NCBITaxon:10088	denotes	mice
T12035	3325-3329	NCBITaxon:10088	denotes	mice
T12036	3340-3344	PR:000004503	denotes	Atrx
T12037	3345-3349	SO:0000359	denotes	flox
T12038	3350-3357	CL:0002322	denotes	ES cell
T12039	3392-3403	UBERON:0000358	denotes	blastocysts
T12040	3507-3512	GO:0007618	denotes	mated
T12041	3581-3585	PR:000004503	denotes	Atrx
T12042	3586-3590	SO:0000359	denotes	flox
T12043	3591-3597	SO:0001023	denotes	allele
T12044	3652-3656	UBERON:0002415	denotes	tail
T12045	3671-3677	SO:0000188	denotes	intron
T12046	3740-3744	PR:000004503	denotes	Atrx
T12047	3745-3749	SO:0000359	denotes	flox
T12048	3750-3754	NCBITaxon:10088	denotes	mice
T12049	3773-3778	PR:000007857	denotes	GATA1
T12050	3794-3798	NCBITaxon:10088	denotes	mice
T12051	3837-3844	SO:0001023	denotes	alleles
T12219	3969-3982	GO:0097617	denotes	hybridisation
T12220	4009-4017	UBERON:0002450	denotes	decidual
T12221	4062-4068	UBERON:0000479	denotes	tissue
T12222	4131-4138	UBERON:0000922	denotes	embryos
T12223	4202-4209	CHEBI:16236	denotes	ethanol
T12224	4221-4227	CHEBI:27338	denotes	xylene
T12225	4492-4498	NCBITaxon:9986	denotes	rabbit
T12226	4510-4520	GO:0042571	denotes	antibodies
T12227	4529-4533	PR:000004503	denotes	ATRX
T12228	4569-4578	UBERON:0001987	denotes	Placental
T12229	4569-4587	CHEBI:81588	denotes	Placental lactogen
T12230	4569-4589	PR:000013543	denotes	Placental lactogen-I
T12231	4664-4671	CHEBI:32958	denotes	phospho
T12232	4680-4687	CHEBI:15358	denotes	histone
T12233	4680-4690	PR:000027594	denotes	histone H3
T12234	4818-4830	CHEBI:51686	denotes	haematoxylin
T12235	4894-4909	CL:0000445	denotes	apoptotic cells
T12236	4937-4947	GO:0008219	denotes	cell death
T12237	5060-5064	CHEBI:51231	denotes	DAPI
T12238	5066-5072	SO:0000440	denotes	Vector
T12239	5189-5203	GO:0097617	denotes	hybridisations
T12240	5230-5237	UBERON:0000922	denotes	embryos
T12241	5272-5294	UBERON:0005292	denotes	extraembryonic tissues
T12242	5304-5313	PR:000016001	denotes	brachyury
T12243	5315-5316	PR:000016001	denotes	T
T12244	5349-5357	UBERON:0002450	denotes	decidual
T12245	5358-5370	GO:0007566	denotes	implantation
T12246	5388-5395	UBERON:0000922	denotes	embryos
T12247	5431-5440	UBERON:0001987	denotes	placental
T12248	5431-5449	CHEBI:81588	denotes	placental lactogen
T12249	5431-5451	PR:000013543	denotes	placental lactogen-1
T12250	5453-5455	CHEBI:81588	denotes	Pl
T12251	5453-5457	PR:000013543	denotes	Pl-1
T12591	5501-5511	UBERON:0000358	denotes	Blastocyst
T12592	5537-5545	GO:0030728	denotes	ovulated
T12593	5566-5570	NCBITaxon:10088	denotes	mice
T12594	5572-5576	PR:000004503	denotes	Atrx
T12595	5580-5584	SO:0000359	denotes	flox
T12596	5591-5596	GO:0007618	denotes	mated
T12597	5611-5616	PR:000007857	denotes	GATA1
T12598	5647-5658	UBERON:0000358	denotes	blastocysts
T12599	5677-5690	UBERON:0002247	denotes	uterine horns
T12600	5737-5748	UBERON:0000358	denotes	blastocysts
T12601	5776-5782	UBERON:0000479	denotes	tissue
T12602	5970-5974	PR:000004503	denotes	Atrx
R6939	T11122	T11121	_lexicallyChainedTo	cells,Generation of

PMC:1440874 / 50062-56120 JSONTXT

Annnotations TAB JSON ListView MergeView

craft-sa-dev

craft-ca-core-ex-dev

2_test

craft-ca-core-dev

PMC:1440874 / 50062-56120 JSON TXT