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PMC:1435744 / 18267-24258 JSONTXT

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craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T12279 20-24 PR_EXT:000014441 denotes mr-s
T12280 25-32 CHEBI_PR_EXT:protein denotes protein
T12281 37-44 SO_EXT:0000417 denotes domains
T12282 70-77 CHEBI_PR_EXT:protein denotes protein
T12283 78-85 CHEBI_PR_EXT:protein denotes protein
T12284 98-105 SO_EXT:0000417 denotes modules
T12285 128-135 SO_EXT:0000417 denotes domains
T12286 140-144 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes bind
T12287 164-170 SO_EXT:0000417 denotes domain
T12288 182-190 CHEBI_PR_EXT:protein denotes proteins
T12289 221-227 SO_EXT:0000417 denotes domain
T12290 292-298 SO_EXT:0000417 denotes domain
T12291 306-310 PR_EXT:000014441 denotes mr-s
T12292 311-318 CHEBI_PR_EXT:protein denotes protein
T12293 342-349 CHEBI_PR_EXT:protein denotes protein
T12294 350-357 CHEBI_PR_EXT:protein denotes protein
T12295 370-376 SO_EXT:0000417 denotes module
T12296 391-396 NCBITaxon_EXT:yeast denotes yeast
T12297 436-440 PR_EXT:000014441 denotes mr-s
T12298 441-448 CHEBI_PR_EXT:protein denotes protein
T12299 495-510 GO_EXT:transcription denotes transcriptional
T12300 521-527 SO_EXT:sequence_fusion_entity_or_process denotes fusion
T12301 528-535 CHEBI_PR_EXT:protein denotes protein
T12302 553-558 NCBITaxon:10088 denotes mouse
T12303 565-572 UBERON:0000966 denotes retinal
T12304 573-578 SO_EXT:cDNA denotes cDNAs
T12305 584-589 SO_EXT:sequence_fusion_process denotes fused
T12306 597-601 PR_EXT:P04386 denotes GAL4
T12307 613-619 SO_EXT:0000417 denotes domain
T12308 648-654 SO_EXT:sequence_cloned_entity denotes clones
T12309 674-678 SO_EXT:cDNA denotes cDNA
T12310 708-714 SO_EXT:0000417 denotes domain
T12311 718-722 PR_EXT:000014441 denotes mr-s
T12312 769-773 PR_EXT:000014441 denotes mr-s
T12313 774-781 CHEBI_PR_EXT:protein denotes protein
T12314 810-816 SO_EXT:0000417 denotes domain
T12315 886-890 PR_EXT:000014441 denotes mr-s
T12316 891-898 CHEBI_PR_EXT:protein denotes protein
T12317 902-907 NCBITaxon_EXT:yeast denotes yeast
T12318 912-917 SO_EXT:sequence_fusion_process denotes fused
T12319 933-942 SO_EXT:sequence_truncation_process denotes truncated
T12320 959-963 PR_EXT:000014441 denotes mr-s
T12321 964-971 CHEBI_PR_EXT:protein denotes protein
T12322 979-982 CHEBI_SO_EXT:DNA denotes DNA
T12323 983-990 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T12324 991-997 SO_EXT:0000417 denotes domain
T12325 1005-1010 NCBITaxon_EXT:yeast denotes yeast
T12326 1011-1024 GO_EXT:transcription denotes transcription
T12327 1011-1031 GO_EXT:transcription_factor denotes transcription factor
T12328 1032-1036 PR_EXT:P04386 denotes GAL4
T12329 1050-1060 SO_EXT:engineered_biological_sequence denotes constructs
T12330 1065-1070 SO_EXT:sequence_fusion_process denotes fused
T12331 1086-1095 SO_EXT:sequence_truncation_process denotes truncated
T12332 1112-1116 PR_EXT:000014441 denotes mr-s
T12333 1117-1124 CHEBI_PR_EXT:protein denotes protein
T12334 1132-1136 PR_EXT:P04386 denotes GAL4
T12335 1137-1152 GO_EXT:transcription denotes transcriptional
T12336 1164-1170 SO_EXT:0000417 denotes domain
T12337 1184-1194 SO_EXT:engineered_biological_sequence denotes constructs
T12338 1212-1222 SO_EXT:engineered_biological_sequence denotes constructs
T12339 1228-1239 GO:0009294 denotes transformed
T12366 1719-1729 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N-terminus
T12367 1734-1736 CHEBI_SO_EXT:amino_acid denotes aa
T12368 1748-1752 PR_EXT:000014441 denotes mr-s
T12369 1784-1794 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N-terminus
T12370 1799-1801 CHEBI_SO_EXT:amino_acid denotes aa
T12371 1802-1806 PR_EXT:000014441 denotes mr-s
T12372 1807-1814 CHEBI_PR_EXT:protein denotes protein
T12373 1832-1836 PR_EXT:000033987 denotes lacZ
T12374 1837-1850 GO_EXT:transcription denotes transcription
T12375 1865-1875 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N-terminus
T12376 1915-1919 PR_EXT:000033987 denotes lacZ
T12377 1920-1930 GO:0010467 denotes expression
T12378 1951-1961 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminus
T12379 1970-1978 SO_EXT:sequence_coding_function denotes encoding
T12380 1991-1993 CHEBI_SO_EXT:amino_acid denotes aa
T12381 2005-2009 PR_EXT:000014441 denotes mr-s
T12382 2042-2046 PR_EXT:P04386 denotes GAL4
T12383 2100-2104 PR_EXT:000014441 denotes mr-s
T12384 2186-2193 SO_EXT:0000417 denotes domains
T12385 2256-2262 SO_EXT:sequence_fusion_entity_or_process denotes fusion
T12386 2263-2271 CHEBI_PR_EXT:protein denotes proteins
T12387 2285-2290 NCBITaxon_EXT:yeast denotes yeast
T12388 2291-2298 GO:0005634 denotes nucleus
T12389 2310-2314 PR_EXT:P04386 denotes GAL4
T12390 2315-2322 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T12391 2315-2328 SO_EXT:0000409 denotes binding sites
T12392 2353-2359 SO_EXT:0000417 denotes domain
T12393 2418-2425 CHEBI_PR_EXT:protein denotes protein
T12419 3920-3932 GO:0009294 denotes transfection
T12420 3953-3959 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T12421 3960-3970 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N-terminus
T12422 3982-3986 PR_EXT:000014441 denotes mr-s
T12423 4039-4045 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T12424 4046-4054 SO_EXT:sequence_deletion_entity_or_process denotes deletion
T12425 4055-4062 SO_EXT:sequence_altered_entity denotes mutants
T12426 4071-4082 GO:0009294 denotes transfected
T12427 4128-4131 PR_EXT:000014441 denotes mrs
T12428 5032-5036 PR_EXT:000014441 denotes mr-s
T12429 5037-5044 CHEBI_PR_EXT:protein denotes protein
T12430 5084-5090 SO_EXT:0000417 denotes domain
T12431 5101-5109 GO_EXT:biological_direction_or_guidance denotes directed
T12432 5110-5119 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T12433 5146-5152 SO_EXT:0000417 denotes domain
T12434 5156-5160 PR_EXT:000014441 denotes mr-s
T12435 5186-5195 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T12436 5196-5201 SO_EXT:sequence_alteration_process denotes alter
T12437 5202-5210 CHEBI_EXT:residue denotes residues
T12438 5220-5229 SO_EXT:biological_conservation_process_or_quality denotes conserved
T12439 5241-5247 SO_EXT:0000417 denotes domain
T12440 5295-5304 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T12441 5346-5353 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T12442 5380-5386 SO_EXT:0000417 denotes domain
T12443 5398-5405 CHEBI_PR_EXT:protein denotes protein
T12444 5407-5425 PR_EXT:Q9VHA0 denotes Sex comb on midleg
T12445 5427-5430 PR_EXT:Q9VHA0 denotes Scm
T12446 5492-5500 GO_EXT:biological_direction_or_guidance denotes directed
T12447 5501-5510 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T12448 5536-5545 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T12449 5563-5570 CHEBI_PR_EXT:protein denotes protein
T12450 5582-5588 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T12451 5601-5605 PR_EXT:000014441 denotes mr-s
T12452 5660-5667 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T12453 5718-5725 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T12454 5778-5781 PR_EXT:000014441 denotes mrs
T12455 5822-5827 NCBITaxon_EXT:yeast denotes yeast
T12456 5839-5843 PR_EXT:P04386 denotes GAL4
T12457 5869-5873 PR_EXT:000014441 denotes mr-s
T12458 5874-5881 CHEBI_PR_EXT:protein denotes protein
T12459 5923-5929 SO_EXT:0000417 denotes domain
T12460 5953-5963 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N-terminus
T12461 5984-5990 SO_EXT:0000417 denotes domain
T30864 2484-2489 NCBITaxon_EXT:yeast denotes yeast
T30865 2515-2519 PR_EXT:P04386 denotes GAL4
T30866 2543-2547 PR_EXT:000014441 denotes mr-s
T30867 2570-2575 NCBITaxon_EXT:yeast denotes yeast
T30868 2610-2616 SO_EXT:sequence_cloned_entity denotes clones
T30869 2654-2658 PR_EXT:000014441 denotes mr-s
T30870 2659-2665 SO_EXT:sequence_cloned_entity denotes clones
T30871 2706-2713 SO_EXT:0000417 denotes domains
T30872 2744-2748 PR_EXT:000014441 denotes mr-s
T30873 2749-2755 SO_EXT:sequence_fusion_entity_or_process denotes fusion
T30874 2756-2764 CHEBI_PR_EXT:protein denotes proteins
T30875 2777-2782 NCBITaxon_EXT:yeast denotes yeast
T30876 2783-2787 PR_EXT:P04386 denotes GAL4
T30877 2837-2844 SO_EXT:0000417 denotes domains
T30878 2865-2869 PR_EXT:000033987 denotes LacZ
T30879 2870-2880 GO:0010467 denotes expression
T30880 3067-3074 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T30881 3075-3081 SO_EXT:0000417 denotes domain
T30882 3098-3104 SO_EXT:0000417 denotes domain
T30883 3124-3128 PR_EXT:000014441 denotes mr-s
T30884 3130-3131 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N
T30885 3133-3143 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N-terminal
T30886 3155-3159 PR_EXT:000014441 denotes mr-s
T30887 3161-3172 CHEBI_SO_EXT:amino_acid denotes amino acids
T30888 3184-3185 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C
T30889 3187-3197 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T30890 3209-3213 PR_EXT:000014441 denotes mr-s
T30891 3215-3226 CHEBI_SO_EXT:amino_acid denotes amino acids
T31382 4268-4272 PR_EXT:000014441 denotes mr-s
T31383 4273-4280 CHEBI_PR_EXT:protein denotes protein
T31384 4303-4312 NCBITaxon:40674 denotes mammalian
T31385 4313-4318 CL_GO_EXT:cell denotes cells
T31386 4349-4359 SO_EXT:engineered_biological_sequence denotes constructs
T31387 4399-4405 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T31388 4414-4420 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T31389 4434-4445 CHEBI_SO_EXT:amino_acid denotes amino acids
T31390 4463-4474 CHEBI_SO_EXT:amino_acid denotes amino acids
T31391 4494-4505 CHEBI_SO_EXT:amino_acid denotes amino acids
T31392 4531-4539 SO_EXT:sequence_insertion_process denotes inserted
T31393 4552-4558 SO_EXT:0000440 denotes vector
T31394 4582-4592 SO_EXT:engineered_biological_sequence denotes constructs
T31395 4614-4625 GO:0009294 denotes transfected
T31396 4639-4644 CL_GO_EXT:cell denotes cells
T31397 4695-4703 GO:0042571 denotes antibody
T31398 4728-4736 GO:0042571 denotes antibody
T31399 4744-4751 CHEBI_PR_EXT:protein denotes protein
T31400 4800-4806 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T31401 4816-4824 GO_EXT:biological_direction_or_guidance denotes directed
T31402 4825-4829 PR_EXT:000014441 denotes mr-s
T31403 4830-4837 SO_EXT:sequence_altered_entity denotes mutants
T31404 4887-4898 GO:0009294 denotes transfected
T31405 4960-4968 GO:0042571 denotes antibody
T31406 4995-5003 GO:0042571 denotes antibody
T12340 1245-1250 NCBITaxon_EXT:yeast denotes yeast
T12341 1266-1275 SO_EXT:0000902 denotes transgene
T12342 1281-1285 PR_EXT:P04386 denotes GAL4
T12343 1286-1293 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T12344 1286-1299 SO_EXT:0000409 denotes binding sites
T12345 1300-1308 SO_EXT:sequence_upstreamness denotes upstream
T12346 1316-1320 PR_EXT:000033987 denotes lacZ
T12347 1321-1325 SO_EXT:0000704 denotes gene
T12348 1357-1361 PR_EXT:000014441 denotes mr-s
T12349 1367-1376 SO_EXT:engineered_biological_sequence denotes construct
T12350 1385-1389 PR_EXT:000033987 denotes lacZ
T12351 1390-1400 GO:0010467 denotes expression
T12352 1422-1426 PR_EXT:000014441 denotes mr-s
T12353 1432-1441 SO_EXT:engineered_biological_sequence denotes construct
T12354 1470-1480 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N-terminus
T12355 1485-1495 CHEBI_SO_EXT:amino_acid denotes amino acid
T12356 1497-1499 CHEBI_SO_EXT:amino_acid denotes aa
T12357 1512-1516 PR_EXT:000014441 denotes mr-s
T12358 1547-1553 SO_EXT:0000417 denotes domain
T12359 1576-1589 GO_EXT:transcription denotes transcription
T12360 1593-1597 PR_EXT:000033987 denotes lacZ
T12361 1623-1633 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N-terminus
T12362 1638-1640 CHEBI_SO_EXT:amino_acid denotes aa
T12363 1652-1656 PR_EXT:000014441 denotes mr-s
T12364 1676-1689 GO_EXT:transcription denotes transcription
T12365 1693-1697 PR_EXT:000033987 denotes lacZ
T12394 3275-3279 PR_EXT:000014441 denotes mr-s
T12395 3280-3287 CHEBI_PR_EXT:protein denotes protein
T12396 3291-3300 NCBITaxon:40674 denotes mammalian
T12397 3301-3306 CL_GO_EXT:cell denotes cells
T12398 3368-3373 CL_GO_EXT:cell denotes cells
T12399 3380-3392 GO:0009294 denotes transfection
T12400 3399-3405 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T12401 3418-3427 SO_EXT:sequence_truncation_process denotes truncated
T12402 3428-3432 PR_EXT:000014441 denotes mr-s
T12403 3442-3448 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T12404 3461-3470 SO_EXT:sequence_truncation_process denotes truncated
T12405 3471-3475 PR_EXT:000014441 denotes mr-s
T12406 3530-3536 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T12407 3537-3551 PR_EXT:000014841 denotes Sonic hedgehog
T12408 3553-3556 PR_EXT:000014841 denotes Shh
T12409 3611-3616 NCBITaxon_EXT:yeast denotes yeast
T12410 3628-3632 PR_EXT:P04386 denotes GAL4
T12411 3643-3649 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T12412 3662-3666 PR_EXT:000014441 denotes mr-s
T12413 3713-3719 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T12414 3732-3736 PR_EXT:000014441 denotes mr-s
T12415 3743-3746 PR_EXT:000014441 denotes mrs
T12416 3761-3767 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T12417 3768-3778 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminus
T12418 3806-3812 SO_EXT:0000417 denotes domain

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T12205 436-440 PR:000014441 denotes mr-s
T12206 553-558 NCBITaxon:10088 denotes mouse
T12207 565-572 UBERON:0000966 denotes retinal
T12208 597-601 PR:P04386 denotes GAL4
T12209 613-619 SO:0000417 denotes domain
T12210 708-714 SO:0000417 denotes domain
T12211 718-722 PR:000014441 denotes mr-s
T12212 769-773 PR:000014441 denotes mr-s
T12213 810-816 SO:0000417 denotes domain
T12214 886-890 PR:000014441 denotes mr-s
T12215 959-963 PR:000014441 denotes mr-s
T12216 991-997 SO:0000417 denotes domain
T12217 1032-1036 PR:P04386 denotes GAL4
T12218 1112-1116 PR:000014441 denotes mr-s
T12219 1132-1136 PR:P04386 denotes GAL4
T12220 1164-1170 SO:0000417 denotes domain
T12221 1228-1239 GO:0009294 denotes transformed
T12222 1266-1275 SO:0000902 denotes transgene
T12223 1281-1285 PR:P04386 denotes GAL4
T12224 1286-1299 SO:0000409 denotes binding sites
T12225 1316-1320 PR:000033987 denotes lacZ
T12226 1321-1325 SO:0000704 denotes gene
T12227 1357-1361 PR:000014441 denotes mr-s
T12228 1385-1389 PR:000033987 denotes lacZ
T12229 1390-1400 GO:0010467 denotes expression
T12230 1422-1426 PR:000014441 denotes mr-s
T12231 1512-1516 PR:000014441 denotes mr-s
T12232 1547-1553 SO:0000417 denotes domain
T12233 1593-1597 PR:000033987 denotes lacZ
T12234 1652-1656 PR:000014441 denotes mr-s
T12235 1693-1697 PR:000033987 denotes lacZ
T12236 1748-1752 PR:000014441 denotes mr-s
T12237 1802-1806 PR:000014441 denotes mr-s
T12238 1832-1836 PR:000033987 denotes lacZ
T12239 1915-1919 PR:000033987 denotes lacZ
T12240 1920-1930 GO:0010467 denotes expression
T12241 2005-2009 PR:000014441 denotes mr-s
T12242 2042-2046 PR:P04386 denotes GAL4
T12243 2100-2104 PR:000014441 denotes mr-s
T12244 2186-2193 SO:0000417 denotes domains
T12245 2291-2298 GO:0005634 denotes nucleus
T12246 2310-2314 PR:P04386 denotes GAL4
T12247 2315-2328 SO:0000409 denotes binding sites
T12248 2353-2359 SO:0000417 denotes domain
T12249 3275-3279 PR:000014441 denotes mr-s
T12250 3291-3300 NCBITaxon:40674 denotes mammalian
T12251 3380-3392 GO:0009294 denotes transfection
T12252 3428-3432 PR:000014441 denotes mr-s
T12253 3471-3475 PR:000014441 denotes mr-s
T12254 3537-3551 PR:000014841 denotes Sonic hedgehog
T12255 3553-3556 PR:000014841 denotes Shh
T12256 3628-3632 PR:P04386 denotes GAL4
T12257 3662-3666 PR:000014441 denotes mr-s
T12258 3732-3736 PR:000014441 denotes mr-s
T12259 3743-3746 PR:000014441 denotes mrs
T12260 3806-3812 SO:0000417 denotes domain
T12261 3920-3932 GO:0009294 denotes transfection
T12262 3982-3986 PR:000014441 denotes mr-s
T12263 4071-4082 GO:0009294 denotes transfected
T12264 4128-4131 PR:000014441 denotes mrs
T12275 5839-5843 PR:P04386 denotes GAL4
T12276 5869-5873 PR:000014441 denotes mr-s
T12277 5923-5929 SO:0000417 denotes domain
T12278 5984-5990 SO:0000417 denotes domain
T30850 2515-2519 PR:P04386 denotes GAL4
T30851 2543-2547 PR:000014441 denotes mr-s
T30852 2654-2658 PR:000014441 denotes mr-s
T30853 2706-2713 SO:0000417 denotes domains
T30854 2744-2748 PR:000014441 denotes mr-s
T30855 2783-2787 PR:P04386 denotes GAL4
T30856 2837-2844 SO:0000417 denotes domains
T30857 2865-2869 PR:000033987 denotes LacZ
T30858 2870-2880 GO:0010467 denotes expression
T30859 3075-3081 SO:0000417 denotes domain
T30860 3098-3104 SO:0000417 denotes domain
T30861 3124-3128 PR:000014441 denotes mr-s
T30862 3155-3159 PR:000014441 denotes mr-s
T30863 3209-3213 PR:000014441 denotes mr-s
T31372 4268-4272 PR:000014441 denotes mr-s
T31373 4303-4312 NCBITaxon:40674 denotes mammalian
T31374 4552-4558 SO:0000440 denotes vector
T31375 4614-4625 GO:0009294 denotes transfected
T31376 4695-4703 GO:0042571 denotes antibody
T31377 4728-4736 GO:0042571 denotes antibody
T31378 4825-4829 PR:000014441 denotes mr-s
T31379 4887-4898 GO:0009294 denotes transfected
T31380 4960-4968 GO:0042571 denotes antibody
T31381 4995-5003 GO:0042571 denotes antibody
T12196 20-24 PR:000014441 denotes mr-s
T12197 37-44 SO:0000417 denotes domains
T12198 98-105 SO:0000417 denotes modules
T12199 128-135 SO:0000417 denotes domains
T12200 164-170 SO:0000417 denotes domain
T12201 221-227 SO:0000417 denotes domain
T12202 292-298 SO:0000417 denotes domain
T12203 306-310 PR:000014441 denotes mr-s
T12204 370-376 SO:0000417 denotes module
T12265 5032-5036 PR:000014441 denotes mr-s
T12266 5084-5090 SO:0000417 denotes domain
T12267 5146-5152 SO:0000417 denotes domain
T12268 5156-5160 PR:000014441 denotes mr-s
T12269 5241-5247 SO:0000417 denotes domain
T12270 5380-5386 SO:0000417 denotes domain
T12271 5407-5425 PR:Q9VHA0 denotes Sex comb on midleg
T12272 5427-5430 PR:Q9VHA0 denotes Scm
T12273 5601-5605 PR:000014441 denotes mr-s
T12274 5778-5781 PR:000014441 denotes mrs

craft-sa-dev

Id Subject Object Predicate Lexical cue
T12462 0-4 NN denotes Self
T12463 5-16 NN denotes association
T12464 4-5 HYPH denotes -
T12465 17-19 IN denotes of
T12466 20-22 NN denotes mr
T12467 23-24 NN denotes s
T12468 22-23 HYPH denotes -
T12469 25-32 NN denotes protein
T12470 32-114 sentence denotes SAM domains are known to function as protein-protein interaction modules [15-17].
T12471 33-36 NN denotes SAM
T12472 37-44 NNS denotes domains
T12473 49-54 VBN denotes known
T12474 45-48 VBP denotes are
T12475 55-57 TO denotes to
T12476 58-66 VB denotes function
T12477 67-69 IN denotes as
T12478 70-77 NN denotes protein
T12479 78-85 NN denotes protein
T12480 77-78 HYPH denotes -
T12481 98-105 NNS denotes modules
T12482 86-97 NN denotes interaction
T12483 106-107 -LRB- denotes [
T12484 107-109 CD denotes 15
T12485 109-110 SYM denotes -
T12486 110-112 CD denotes 17
T12487 112-113 -RRB- denotes ]
T12488 113-114 . denotes .
T12489 114-260 sentence denotes Although SAM domains can bind to various non-SAM domain-containing proteins, many homo-SAM and hetero-SAM domain interactions have been reported.
T12490 115-123 IN denotes Although
T12491 140-144 VB denotes bind
T12492 124-127 NN denotes SAM
T12493 128-135 NNS denotes domains
T12494 136-139 MD denotes can
T12495 251-259 VBN denotes reported
T12496 145-147 IN denotes to
T12497 148-155 JJ denotes various
T12498 182-190 NN denotes proteins
T12499 156-163 JJ denotes non-SAM
T12500 164-170 NN denotes domain
T12501 171-181 VBG denotes containing
T12502 170-171 HYPH denotes -
T12503 190-192 , denotes ,
T12504 192-196 JJ denotes many
T12505 228-240 NNS denotes interactions
T12506 197-205 NN denotes homo-SAM
T12507 206-209 CC denotes and
T12508 210-220 NN denotes hetero-SAM
T12509 221-227 NN denotes domain
T12510 241-245 VBP denotes have
T12511 246-250 VBN denotes been
T12512 259-260 . denotes .
T12513 260-461 sentence denotes To investigate whether the SAM domain of the mr-s protein can also function as a protein-protein interaction module, we performed yeast two-hybrid screening using full-length mr-s protein as the bait.
T12514 261-263 TO denotes To
T12515 264-275 VB denotes investigate
T12516 381-390 VBD denotes performed
T12517 276-283 IN denotes whether
T12518 328-336 VB denotes function
T12519 284-287 DT denotes the
T12520 292-298 NN denotes domain
T12521 288-291 NN denotes SAM
T12522 299-301 IN denotes of
T12523 302-305 DT denotes the
T12524 311-318 NN denotes protein
T12525 306-308 NN denotes mr
T12526 309-310 NN denotes s
T12527 308-309 HYPH denotes -
T12528 319-322 MD denotes can
T12529 323-327 RB denotes also
T12530 337-339 IN denotes as
T12531 340-341 DT denotes a
T12532 370-376 NN denotes module
T12533 342-349 NN denotes protein
T12534 350-357 NN denotes protein
T12535 349-350 HYPH denotes -
T12536 358-369 NN denotes interaction
T12537 376-378 , denotes ,
T12538 378-380 PRP denotes we
T12539 391-396 NN denotes yeast
T12540 408-417 NN denotes screening
T12541 397-400 CD denotes two
T12542 401-407 NN denotes hybrid
T12543 400-401 HYPH denotes -
T12544 418-423 VBG denotes using
T12545 424-428 JJ denotes full
T12546 429-435 NN denotes length
T12547 428-429 HYPH denotes -
T12548 441-448 NN denotes protein
T12549 436-438 NN denotes mr
T12550 439-440 NN denotes s
T12551 438-439 HYPH denotes -
T12552 449-451 IN denotes as
T12553 452-455 DT denotes the
T12554 456-460 NN denotes bait
T12555 460-461 . denotes .
T12556 461-620 sentence denotes Using this bait, we screened the transcriptional activator fusion protein library in which mouse P0-P3 retinal cDNAs were fused to the GAL4 activation domain.
T12557 462-467 VBG denotes Using
T12558 482-490 VBD denotes screened
T12559 468-472 DT denotes this
T12560 473-477 NN denotes bait
T12561 477-479 , denotes ,
T12562 479-481 PRP denotes we
T12563 491-494 DT denotes the
T12564 536-543 NN denotes library
T12565 495-510 JJ denotes transcriptional
T12566 511-520 NN denotes activator
T12567 528-535 NN denotes protein
T12568 521-527 NN denotes fusion
T12569 544-546 IN denotes in
T12570 584-589 VBN denotes fused
T12571 547-552 WDT denotes which
T12572 553-558 NN denotes mouse
T12573 573-578 NNS denotes cDNAs
T12574 559-561 NN denotes P0
T12575 561-562 SYM denotes -
T12576 562-564 NN denotes P3
T12577 565-572 JJ denotes retinal
T12578 579-583 VBD denotes were
T12579 590-592 IN denotes to
T12580 593-596 DT denotes the
T12581 613-619 NN denotes domain
T12582 597-601 NN denotes GAL4
T12583 602-612 NN denotes activation
T12584 619-620 . denotes .
T12585 620-733 sentence denotes The most frequent positive clones (5 out of 28) were cDNA fragments containing the SAM domain of mr-s (Fig. 4A).
T12586 621-624 DT denotes The
T12587 648-654 NNS denotes clones
T12588 625-629 RBS denotes most
T12589 630-638 JJ denotes frequent
T12590 639-647 JJ denotes positive
T12591 669-673 VBD denotes were
T12592 655-656 -LRB- denotes (
T12593 665-667 CD denotes 28
T12594 656-657 CD denotes 5
T12595 658-661 IN denotes out
T12596 662-664 IN denotes of
T12597 667-668 -RRB- denotes )
T12598 674-678 NN denotes cDNA
T12599 679-688 NNS denotes fragments
T12600 689-699 VBG denotes containing
T12601 700-703 DT denotes the
T12602 708-714 NN denotes domain
T12603 704-707 NN denotes SAM
T12604 715-717 IN denotes of
T12605 718-720 NN denotes mr
T12606 721-722 NN denotes s
T12607 720-721 HYPH denotes -
T12608 723-724 -LRB- denotes (
T12609 729-731 NN denotes 4A
T12610 724-728 NN denotes Fig.
T12611 731-732 -RRB- denotes )
T12612 732-733 . denotes .
T12613 733-836 sentence denotes This result strongly suggests that mr-s protein self-associates through SAM domain-containing regions.
T12614 734-738 DT denotes This
T12615 739-745 NN denotes result
T12616 755-763 VBZ denotes suggests
T12617 746-754 RB denotes strongly
T12618 764-768 IN denotes that
T12619 787-797 VBZ denotes associates
T12620 769-771 NN denotes mr
T12621 772-773 NN denotes s
T12622 771-772 HYPH denotes -
T12623 774-781 NN denotes protein
T12624 782-786 NN denotes self
T12625 786-787 HYPH denotes -
T12626 798-805 IN denotes through
T12627 806-809 NN denotes SAM
T12628 810-816 NN denotes domain
T12629 817-827 VBG denotes containing
T12630 816-817 HYPH denotes -
T12631 828-835 NNS denotes regions
T12632 835-836 . denotes .
T12633 836-908 sentence denotes We then directly tested this self-association of mr-s protein in yeast.
T12634 837-839 PRP denotes We
T12635 854-860 VBN denotes tested
T12636 840-844 RB denotes then
T12637 845-853 RB denotes directly
T12638 861-865 DT denotes this
T12639 871-882 NN denotes association
T12640 866-870 NN denotes self
T12641 870-871 HYPH denotes -
T12642 883-885 IN denotes of
T12643 886-888 NN denotes mr
T12644 889-890 NN denotes s
T12645 888-889 HYPH denotes -
T12646 891-898 NN denotes protein
T12647 899-901 IN denotes in
T12648 902-907 NN denotes yeast
T12649 907-908 . denotes .
T12650 908-1061 sentence denotes We fused full-length or truncated portions of the mr-s protein to the DNA-binding domain of the yeast transcription factor GAL4 to make bait constructs.
T12651 909-911 PRP denotes We
T12652 912-917 VBD denotes fused
T12653 918-922 JJ denotes full
T12654 923-929 NN denotes length
T12655 922-923 HYPH denotes -
T12656 943-951 NNS denotes portions
T12657 930-932 CC denotes or
T12658 933-942 VBN denotes truncated
T12659 952-954 IN denotes of
T12660 955-958 DT denotes the
T12661 964-971 NN denotes protein
T12662 959-961 NN denotes mr
T12663 962-963 NN denotes s
T12664 961-962 HYPH denotes -
T12665 972-974 IN denotes to
T12666 975-978 DT denotes the
T12667 991-997 NN denotes domain
T12668 979-982 NN denotes DNA
T12669 983-990 VBG denotes binding
T12670 982-983 HYPH denotes -
T12671 998-1000 IN denotes of
T12672 1001-1004 DT denotes the
T12673 1025-1031 NN denotes factor
T12674 1005-1010 NN denotes yeast
T12675 1011-1024 NN denotes transcription
T12676 1032-1036 NN denotes GAL4
T12677 1037-1039 TO denotes to
T12678 1040-1044 VB denotes make
T12679 1045-1049 NN denotes bait
T12680 1050-1060 NNS denotes constructs
T12681 1060-1061 . denotes .
T12682 1061-1205 sentence denotes We fused full-length or truncated portions of the mr-s protein to the GAL4 transcriptional activation domain to make prey constructs (Fig. 4B).
T12683 1062-1064 PRP denotes We
T12684 1065-1070 VBD denotes fused
T12685 1071-1075 JJ denotes full
T12686 1076-1082 NN denotes length
T12687 1075-1076 HYPH denotes -
T12688 1096-1104 NNS denotes portions
T12689 1083-1085 CC denotes or
T12690 1086-1095 VBN denotes truncated
T12691 1105-1107 IN denotes of
T12692 1108-1111 DT denotes the
T12693 1117-1124 NN denotes protein
T12694 1112-1114 NN denotes mr
T12695 1115-1116 NN denotes s
T12696 1114-1115 HYPH denotes -
T12697 1125-1127 IN denotes to
T12698 1128-1131 DT denotes the
T12699 1164-1170 NN denotes domain
T12700 1132-1136 NN denotes GAL4
T12701 1153-1163 NN denotes activation
T12702 1137-1152 JJ denotes transcriptional
T12703 1171-1173 TO denotes to
T12704 1174-1178 VB denotes make
T12705 1179-1183 NN denotes prey
T12706 1184-1194 NNS denotes constructs
T12707 1195-1196 -LRB- denotes (
T12708 1201-1203 NN denotes 4B
T12709 1196-1200 NN denotes Fig.
T12710 1203-1204 -RRB- denotes )
T12711 1204-1205 . denotes .
T12712 1205-1326 sentence denotes These constructs were transformed into yeast that contain a transgene with GAL4 binding sites upstream of the lacZ gene.
T12713 1206-1211 DT denotes These
T12714 1212-1222 NNS denotes constructs
T12715 1228-1239 VBN denotes transformed
T12716 1223-1227 VBD denotes were
T12717 1240-1244 IN denotes into
T12718 1245-1250 NN denotes yeast
T12719 1251-1255 WDT denotes that
T12720 1256-1263 VBP denotes contain
T12721 1264-1265 DT denotes a
T12722 1266-1275 NN denotes transgene
T12723 1276-1280 IN denotes with
T12724 1281-1285 NN denotes GAL4
T12725 1286-1293 NN denotes binding
T12726 1294-1299 NNS denotes sites
T12727 1300-1308 RB denotes upstream
T12728 1309-1311 IN denotes of
T12729 1312-1315 DT denotes the
T12730 1321-1325 NN denotes gene
T12731 1316-1320 NN denotes lacZ
T12732 1325-1326 . denotes .
T12733 1326-1465 sentence denotes We found that the full-length mr-s bait construct induced lacZ expression with the full-length mr-s prey construct (Fig. 4B, full × full).
T12734 1327-1329 PRP denotes We
T12735 1330-1335 VBD denotes found
T12736 1336-1340 IN denotes that
T12737 1377-1384 VBD denotes induced
T12738 1341-1344 DT denotes the
T12739 1367-1376 NN denotes construct
T12740 1345-1349 JJ denotes full
T12741 1350-1356 NN denotes length
T12742 1349-1350 HYPH denotes -
T12743 1357-1359 NN denotes mr
T12744 1360-1361 NN denotes s
T12745 1359-1360 HYPH denotes -
T12746 1362-1366 NN denotes bait
T12747 1385-1389 NN denotes lacZ
T12748 1390-1400 NN denotes expression
T12749 1401-1405 IN denotes with
T12750 1406-1409 DT denotes the
T12751 1432-1441 NN denotes construct
T12752 1410-1414 JJ denotes full
T12753 1415-1421 NN denotes length
T12754 1414-1415 HYPH denotes -
T12755 1422-1424 NN denotes mr
T12756 1425-1426 NN denotes s
T12757 1424-1425 HYPH denotes -
T12758 1427-1431 NN denotes prey
T12759 1442-1443 -LRB- denotes (
T12760 1459-1463 JJ denotes full
T12761 1443-1447 NN denotes Fig.
T12762 1448-1450 NN denotes 4B
T12763 1450-1452 , denotes ,
T12764 1452-1456 JJ denotes full
T12765 1457-1458 SYM denotes ×
T12766 1463-1464 -RRB- denotes )
T12767 1464-1465 . denotes .
T12768 1465-1618 sentence denotes The N-terminus 400 amino acid (aa) stretch of mr-s, which does not contain a SAM domain, does weakly activate transcription of lacZ (Fig. 4B, full × N).
T12769 1466-1469 DT denotes The
T12770 1501-1508 NN denotes stretch
T12771 1470-1471 NN denotes N
T12772 1472-1480 NN denotes terminus
T12773 1471-1472 HYPH denotes -
T12774 1481-1484 CD denotes 400
T12775 1485-1490 NN denotes amino
T12776 1491-1495 NN denotes acid
T12777 1496-1497 -LRB- denotes (
T12778 1497-1499 NN denotes aa
T12779 1499-1500 -RRB- denotes )
T12780 1567-1575 VB denotes activate
T12781 1509-1511 IN denotes of
T12782 1512-1514 NN denotes mr
T12783 1515-1516 NN denotes s
T12784 1514-1515 HYPH denotes -
T12785 1516-1518 , denotes ,
T12786 1518-1523 WDT denotes which
T12787 1533-1540 VB denotes contain
T12788 1524-1528 VBZ denotes does
T12789 1529-1532 RB denotes not
T12790 1541-1542 DT denotes a
T12791 1547-1553 NN denotes domain
T12792 1543-1546 NN denotes SAM
T12793 1553-1555 , denotes ,
T12795 1560-1566 RB denotes weakly
T12796 1576-1589 NN denotes transcription
T12797 1590-1592 IN denotes of
T12798 1593-1597 NN denotes lacZ
T12799 1598-1599 -LRB- denotes (
T12800 1615-1616 NN denotes N
T12801 1599-1603 NN denotes Fig.
T12802 1604-1606 NN denotes 4B
T12803 1606-1608 , denotes ,
T12804 1608-1612 JJ denotes full
T12805 1613-1614 SYM denotes ×
T12806 1616-1617 -RRB- denotes )
T12807 1617-1618 . denotes .
T12808 1618-1770 sentence denotes The N-terminus 400 aa stretch of mr-s was able to induce transcription of lacZ weakly with the same N-terminus 400 aa stretch of mr-s (Fig. 4B, N × N).
T12809 1619-1622 DT denotes The
T12810 1641-1648 NN denotes stretch
T12811 1623-1624 NN denotes N
T12812 1625-1633 NN denotes terminus
T12813 1624-1625 HYPH denotes -
T12814 1634-1637 CD denotes 400
T12815 1638-1640 NN denotes aa
T12816 1657-1660 VBD denotes was
T12817 1649-1651 IN denotes of
T12818 1652-1654 NN denotes mr
T12819 1655-1656 NN denotes s
T12820 1654-1655 HYPH denotes -
T12821 1661-1665 JJ denotes able
T12822 1666-1668 TO denotes to
T12823 1669-1675 VB denotes induce
T12824 1676-1689 NN denotes transcription
T12825 1690-1692 IN denotes of
T12826 1693-1697 NN denotes lacZ
T12827 1698-1704 RB denotes weakly
T12828 1705-1709 IN denotes with
T12829 1710-1713 DT denotes the
T12830 1737-1744 NN denotes stretch
T12831 1714-1718 JJ denotes same
T12832 1719-1720 NN denotes N
T12833 1721-1729 NN denotes terminus
T12834 1720-1721 HYPH denotes -
T12835 1730-1733 CD denotes 400
T12836 1734-1736 NN denotes aa
T12837 1745-1747 IN denotes of
T12838 1748-1750 NN denotes mr
T12839 1751-1752 NN denotes s
T12840 1750-1751 HYPH denotes -
T12841 1753-1754 -LRB- denotes (
T12842 1767-1768 NN denotes N
T12843 1754-1758 NN denotes Fig.
T12844 1759-1761 NN denotes 4B
T12845 1761-1763 , denotes ,
T12846 1763-1764 NN denotes N
T12847 1765-1766 SYM denotes ×
T12848 1768-1769 -RRB- denotes )
T12849 1769-1770 . denotes .
T12850 1770-2037 sentence denotes Although the N-terminus 400 aa mr-s protein weakly activates lacZ transcription with the same N-terminus portion, a much stronger activation of lacZ expression was observed with a C-terminus portion encoding the 391–542 aa stretch of mr-s (Fig. 4B, full × C, C × C).
T12851 1771-1779 IN denotes Although
T12852 1822-1831 VBZ denotes activates
T12853 1780-1783 DT denotes the
T12854 1807-1814 NN denotes protein
T12855 1784-1785 NN denotes N
T12856 1786-1794 NN denotes terminus
T12857 1785-1786 HYPH denotes -
T12858 1795-1798 CD denotes 400
T12859 1799-1801 NN denotes aa
T12860 1802-1804 NN denotes mr
T12861 1805-1806 NN denotes s
T12862 1804-1805 HYPH denotes -
T12863 1815-1821 RB denotes weakly
T12864 1935-1943 VBN denotes observed
T12865 1832-1836 NN denotes lacZ
T12866 1837-1850 NN denotes transcription
T12867 1851-1855 IN denotes with
T12868 1856-1859 DT denotes the
T12869 1876-1883 NN denotes portion
T12870 1860-1864 JJ denotes same
T12871 1865-1866 NN denotes N
T12872 1867-1875 NN denotes terminus
T12873 1866-1867 HYPH denotes -
T12874 1883-1885 , denotes ,
T12875 1885-1886 DT denotes a
T12876 1901-1911 NN denotes activation
T12877 1887-1891 RB denotes much
T12878 1892-1900 JJR denotes stronger
T12879 1912-1914 IN denotes of
T12880 1915-1919 NN denotes lacZ
T12881 1920-1930 NN denotes expression
T12882 1931-1934 VBD denotes was
T12883 1944-1948 IN denotes with
T12884 1949-1950 DT denotes a
T12885 1962-1969 NN denotes portion
T12886 1951-1952 NN denotes C
T12887 1953-1961 NN denotes terminus
T12888 1952-1953 HYPH denotes -
T12889 1970-1978 VBG denotes encoding
T12890 1979-1982 DT denotes the
T12891 1994-2001 NN denotes stretch
T12892 1983-1986 CD denotes 391
T12893 1991-1993 NN denotes aa
T12894 1986-1987 SYM denotes –
T12895 1987-1990 CD denotes 542
T12896 2002-2004 IN denotes of
T12897 2005-2007 NN denotes mr
T12898 2008-2009 NN denotes s
T12905 2020-2024 JJ denotes full
T12906 2025-2026 SYM denotes ×
T12907 2028-2030 , denotes ,
T12908 2030-2031 NN denotes C
T12909 2034-2035 NN denotes C
T12910 2032-2033 SYM denotes ×
T12911 2035-2036 -RRB- denotes )
T12912 2036-2037 . denotes .
T12913 2037-2204 sentence denotes Our GAL4 assay indicated that the signal when the full-length mr-s was present in both the bait and prey contexts was weaker than when isolated SAM domains were used.
T12914 2038-2041 PRP$ denotes Our
T12915 2047-2052 NN denotes assay
T12916 2042-2046 NN denotes GAL4
T12917 2053-2062 VBD denotes indicated
T12918 2063-2067 IN denotes that
T12919 2152-2155 VBD denotes was
T12920 2068-2071 DT denotes the
T12921 2072-2078 NN denotes signal
T12922 2079-2083 WRB denotes when
T12923 2105-2108 VBD denotes was
T12924 2084-2087 DT denotes the
T12925 2103-2104 NN denotes s
T12926 2088-2092 JJ denotes full
T12927 2093-2099 NN denotes length
T12928 2092-2093 HYPH denotes -
T12929 2100-2102 NN denotes mr
T12930 2102-2103 HYPH denotes -
T12931 2109-2116 JJ denotes present
T12932 2117-2119 IN denotes in
T12933 2120-2124 CC denotes both
T12934 2143-2151 NNS denotes contexts
T12935 2125-2128 DT denotes the
T12936 2129-2133 NN denotes bait
T12937 2134-2137 CC denotes and
T12938 2138-2142 NN denotes prey
T12939 2156-2162 JJR denotes weaker
T12940 2163-2167 IN denotes than
T12941 2168-2172 WRB denotes when
T12942 2199-2203 VBN denotes used
T12943 2173-2181 JJ denotes isolated
T12944 2186-2193 NNS denotes domains
T12945 2182-2185 NN denotes SAM
T12946 2194-2198 VBD denotes were
T12947 2203-2204 . denotes .
T12948 2204-2329 sentence denotes This may simply reflect the tendency for the small fusion proteins to enter the yeast nucleus and occupy GAL4 binding sites.
T12949 2205-2209 DT denotes This
T12950 2221-2228 VB denotes reflect
T12951 2210-2213 MD denotes may
T12952 2214-2220 RB denotes simply
T12953 2229-2232 DT denotes the
T12954 2233-2241 NN denotes tendency
T12955 2242-2245 IN denotes for
T12956 2275-2280 VB denotes enter
T12957 2246-2249 DT denotes the
T12958 2263-2271 NN denotes proteins
T12959 2250-2255 JJ denotes small
T12960 2256-2262 NN denotes fusion
T12961 2272-2274 TO denotes to
T12962 2281-2284 DT denotes the
T12963 2291-2298 NN denotes nucleus
T12964 2285-2290 NN denotes yeast
T12965 2299-2302 CC denotes and
T12966 2303-2309 VB denotes occupy
T12967 2310-2314 NN denotes GAL4
T12968 2315-2322 NN denotes binding
T12969 2323-2328 NNS denotes sites
T12970 2328-2329 . denotes .
T12971 2329-2462 sentence denotes Alternatively, the SAM domain may be less accessible for interaction in the full-length protein context as previously reported [39].
T12972 2330-2343 RB denotes Alternatively
T12973 2364-2366 VB denotes be
T12974 2343-2345 , denotes ,
T12975 2345-2348 DT denotes the
T12976 2353-2359 NN denotes domain
T12977 2349-2352 NN denotes SAM
T12978 2360-2363 MD denotes may
T12979 2367-2371 RBR denotes less
T12980 2372-2382 JJ denotes accessible
T12981 2383-2386 IN denotes for
T12982 2387-2398 NN denotes interaction
T12983 2399-2401 IN denotes in
T12984 2402-2405 DT denotes the
T12985 2426-2433 NN denotes context
T12986 2406-2410 JJ denotes full
T12987 2411-2417 NN denotes length
T12988 2410-2411 HYPH denotes -
T12989 2418-2425 NN denotes protein
T12990 2434-2436 IN denotes as
T12991 2448-2456 VBN denotes reported
T12992 2437-2447 RB denotes previously
T12993 2457-2458 -LRB- denotes [
T12994 2458-2460 CD denotes 39
T12995 2460-2461 -RRB- denotes ]
T12996 2461-2462 . denotes .
T12997 2462-2463 sentence denotes
T13027 3398-3399 HYPH denotes -
T13028 3431-3432 NN denotes s
T13029 3406-3410 JJ denotes full
T13030 3411-3417 NN denotes length
T13031 3410-3411 HYPH denotes -
T13032 3418-3427 VBN denotes truncated
T13033 3417-3418 HYPH denotes /
T13034 3428-3430 NN denotes mr
T13035 3430-3431 HYPH denotes -
T13036 3433-3436 CC denotes and
T13037 3437-3441 NN denotes Flag
T13038 3442-3448 VBN denotes tagged
T13039 3441-3442 HYPH denotes -
T13040 3474-3475 NN denotes s
T13041 3449-3453 JJ denotes full
T13042 3454-3460 NN denotes length
T13043 3453-3454 HYPH denotes -
T13044 3461-3470 VBN denotes truncated
T13045 3460-3461 HYPH denotes /
T13046 3471-3473 NN denotes mr
T13047 3473-3474 HYPH denotes -
T13048 3476-3477 -LRB- denotes (
T13049 3482-3484 NN denotes 5A
T13050 3477-3481 NN denotes Fig.
T13051 3484-3485 -RRB- denotes )
T13052 3485-3486 . denotes .
T13053 3486-3573 sentence denotes As a negative control, we constructed Flag-tagged Sonic hedgehog (Shh) (lane 2 and 7).
T13054 3487-3489 IN denotes As
T13055 3513-3524 VBD denotes constructed
T13056 3490-3491 DT denotes a
T13057 3501-3508 NN denotes control
T13058 3492-3500 JJ denotes negative
T13059 3508-3510 , denotes ,
T13060 3510-3512 PRP denotes we
T13061 3525-3529 NN denotes Flag
T13062 3530-3536 VBN denotes tagged
T13063 3529-3530 HYPH denotes -
T13064 3543-3551 NN denotes hedgehog
T13065 3537-3542 JJ denotes Sonic
T13066 3552-3553 -LRB- denotes (
T13067 3553-3556 NN denotes Shh
T13068 3556-3557 -RRB- denotes )
T13069 3558-3559 -LRB- denotes (
T13070 3564-3565 CD denotes 2
T13071 3559-3563 NN denotes lane
T13072 3566-3569 CC denotes and
T13073 3570-3571 CD denotes 7
T13074 3571-3572 -RRB- denotes )
T13075 3572-3573 . denotes .
T13076 3573-3864 sentence denotes In accordance with the result of the yeast two-hybrid GAL4 assay, HA-tagged full-length mr-s (full-HA) was co-immunoprecipitated with Flag-tagged full-length mr-s (Flag-mrs) and the Flag-tagged C-terminus portion containing the SAM domain (Flag-SAM), respectively (Fig 5B, lane3 and lane5).
T13077 3574-3576 IN denotes In
T13078 3681-3702 VBN denotes co-immunoprecipitated
T13079 3577-3587 NN denotes accordance
T13080 3588-3592 IN denotes with
T13081 3593-3596 DT denotes the
T13082 3597-3603 NN denotes result
T13083 3604-3606 IN denotes of
T13084 3607-3610 DT denotes the
T13085 3633-3638 NN denotes assay
T13086 3611-3616 NN denotes yeast
T13087 3617-3620 CD denotes two
T13088 3621-3627 NN denotes hybrid
T13089 3620-3621 HYPH denotes -
T13090 3628-3632 NN denotes GAL4
T13091 3638-3640 , denotes ,
T13092 3640-3642 NN denotes HA
T13093 3643-3649 VBN denotes tagged
T13094 3642-3643 HYPH denotes -
T13095 3665-3666 NN denotes s
T13096 3650-3654 JJ denotes full
T13097 3655-3661 NN denotes length
T13098 3654-3655 HYPH denotes -
T13099 3662-3664 NN denotes mr
T13100 3664-3665 HYPH denotes -
T13101 3667-3668 -LRB- denotes (
T13102 3668-3672 JJ denotes full
T13103 3673-3675 NN denotes HA
T13104 3672-3673 HYPH denotes -
T13105 3675-3676 -RRB- denotes )
T13106 3677-3680 VBD denotes was
T13107 3703-3707 IN denotes with
T13108 3708-3712 NN denotes Flag
T13109 3713-3719 VBN denotes tagged
T13110 3712-3713 HYPH denotes -
T13111 3735-3736 NN denotes s
T13112 3720-3724 JJ denotes full
T13113 3725-3731 NN denotes length
T13114 3724-3725 HYPH denotes -
T13115 3732-3734 NN denotes mr
T13116 3734-3735 HYPH denotes -
T13117 3737-3738 -LRB- denotes (
T13118 3738-3742 NN denotes Flag
T13119 3743-3746 NN denotes mrs
T13120 3742-3743 HYPH denotes -
T13121 3746-3747 -RRB- denotes )
T13122 3748-3751 CC denotes and
T13123 3752-3755 DT denotes the
T13124 3779-3786 NN denotes portion
T13125 3756-3760 NN denotes Flag
T13126 3761-3767 VBN denotes tagged
T13127 3760-3761 HYPH denotes -
T13128 3768-3769 NN denotes C
T13129 3770-3778 NN denotes terminus
T13130 3769-3770 HYPH denotes -
T13131 3787-3797 VBG denotes containing
T13132 3798-3801 DT denotes the
T13133 3806-3812 NN denotes domain
T13134 3802-3805 NN denotes SAM
T13135 3813-3814 -LRB- denotes (
T13136 3814-3818 NN denotes Flag
T13137 3819-3822 NN denotes SAM
T13138 3818-3819 HYPH denotes -
T13139 3822-3823 -RRB- denotes )
T13140 3823-3825 , denotes ,
T13141 3825-3837 RB denotes respectively
T13142 3838-3839 -LRB- denotes (
T13143 3847-3852 NN denotes lane3
T13144 3839-3842 NN denotes Fig
T13145 3843-3845 NN denotes 5B
T13146 3845-3847 , denotes ,
T13147 3853-3856 CC denotes and
T13148 3857-3862 NN denotes lane5
T13149 3862-3863 -RRB- denotes )
T13150 3863-3864 . denotes .
T13151 3864-4016 sentence denotes We also found a weak co-immunoprecipitation band in co-transfection of full-HA and Flag-tagged N-terminus portion of mr-s (Flag-ΔSAM, Fig. 5B, lane 4).
T13152 3865-3867 PRP denotes We
T13153 3873-3878 VBD denotes found
T13154 3868-3872 RB denotes also
T13155 3879-3880 DT denotes a
T13156 3909-3913 NN denotes band
T13157 3881-3885 JJ denotes weak
T13158 3886-3908 NN denotes co-immunoprecipitation
T13159 3914-3916 IN denotes in
T13160 3917-3932 NN denotes co-transfection
T13161 3933-3935 IN denotes of
T13162 3936-3940 JJ denotes full
T13163 3941-3943 NN denotes HA
T13164 3940-3941 HYPH denotes -
T13165 3944-3947 CC denotes and
T13166 3948-3952 NN denotes Flag
T13167 3953-3959 VBN denotes tagged
T13168 3952-3953 HYPH denotes -
T13169 3971-3978 NN denotes portion
T13170 3960-3961 NN denotes N
T13171 3962-3970 NN denotes terminus
T13172 3961-3962 HYPH denotes -
T13173 3979-3981 IN denotes of
T13174 3982-3984 NN denotes mr
T13175 3985-3986 NN denotes s
T13176 3984-3985 HYPH denotes -
T13177 3987-3988 -LRB- denotes (
T13178 3988-3992 NN denotes Flag
T13179 3993-3997 NN denotes ΔSAM
T13180 3992-3993 HYPH denotes -
T13181 3997-3999 , denotes ,
T13182 4008-4012 NN denotes lane
T13183 3999-4003 NN denotes Fig.
T13184 4004-4006 NN denotes 5B
T13185 4006-4008 , denotes ,
T13186 4013-4014 CD denotes 4
T13187 4014-4015 -RRB- denotes )
T13188 4015-4016 . denotes .
T13189 4016-4253 sentence denotes When ΔSAM-HA and Flag-tagged deletion mutants were co-transfected, ΔSAM-HA was co-immunoprecipitated with Flag-mrs and Flag-ΔSAM (Fig. 5B, lane 8 and lane 9), while ΔSAM-HA was not co-immunoprecipitated with Flag-SAM (Fig. 5B, lane 10).
T13190 4017-4021 WRB denotes When
T13191 4068-4082 VBN denotes co-transfected
T13192 4022-4026 NN denotes ΔSAM
T13193 4027-4029 NN denotes HA
T13194 4026-4027 HYPH denotes -
T13195 4030-4033 CC denotes and
T13196 4034-4038 NN denotes Flag
T13197 4039-4045 VBN denotes tagged
T13198 4038-4039 HYPH denotes -
T13199 4055-4062 NNS denotes mutants
T13200 4046-4054 NN denotes deletion
T13201 4063-4067 VBD denotes were
T13202 4096-4117 VBN denotes co-immunoprecipitated
T13203 4082-4084 , denotes ,
T13204 4084-4088 NN denotes ΔSAM
T13205 4089-4091 NN denotes HA
T13206 4088-4089 HYPH denotes -
T13207 4092-4095 VBD denotes was
T13208 4118-4122 IN denotes with
T13209 4123-4127 NN denotes Flag
T13210 4128-4131 NN denotes mrs
T13211 4127-4128 HYPH denotes -
T13212 4132-4135 CC denotes and
T13213 4136-4140 NN denotes Flag
T13214 4141-4145 NN denotes ΔSAM
T13215 4140-4141 HYPH denotes -
T13216 4146-4147 -LRB- denotes (
T13217 4152-4154 NN denotes 5B
T13218 4147-4151 NN denotes Fig.
T13219 4154-4156 , denotes ,
T13220 4156-4160 NN denotes lane
T13221 4161-4162 CD denotes 8
T13222 4163-4166 CC denotes and
T13223 4167-4171 NN denotes lane
T13224 4172-4173 CD denotes 9
T13225 4173-4174 -RRB- denotes )
T13226 4174-4176 , denotes ,
T13227 4176-4181 IN denotes while
T13228 4198-4219 VBN denotes co-immunoprecipitated
T13229 4182-4186 NN denotes ΔSAM
T13230 4187-4189 NN denotes HA
T13231 4186-4187 HYPH denotes -
T13232 4190-4193 VBD denotes was
T13233 4194-4197 RB denotes not
T13234 4220-4224 IN denotes with
T13235 4225-4229 NN denotes Flag
T13284 5177-5178 -RRB- denotes )
T13285 5178-5179 . denotes .
T13286 5179-5437 sentence denotes These mutations alter residues that are conserved in the SAM domain of ph and previous report indicates that these mutations of ph-SAM cause significant reduction in binding activity to the other SAM domain-containing protein, Sex comb on midleg (Scm) (41).
T13287 5180-5185 DT denotes These
T13288 5186-5195 NNS denotes mutations
T13289 5196-5201 VBP denotes alter
T13290 5202-5210 NNS denotes residues
T13291 5211-5215 WDT denotes that
T13292 5220-5229 VBN denotes conserved
T13293 5216-5219 VBP denotes are
T13294 5230-5232 IN denotes in
T13295 5233-5236 DT denotes the
T13296 5241-5247 NN denotes domain
T13297 5237-5240 NN denotes SAM
T13298 5248-5250 IN denotes of
T13299 5251-5253 NN denotes ph
T13300 5254-5257 CC denotes and
T13301 5258-5266 JJ denotes previous
T13302 5267-5273 NN denotes report
T13303 5274-5283 VBZ denotes indicates
T13304 5284-5288 IN denotes that
T13305 5315-5320 VBP denotes cause
T13306 5289-5294 DT denotes these
T13307 5295-5304 NNS denotes mutations
T13308 5305-5307 IN denotes of
T13309 5308-5310 NN denotes ph
T13310 5311-5314 NN denotes SAM
T13311 5310-5311 HYPH denotes -
T13312 5321-5332 JJ denotes significant
T13313 5333-5342 NN denotes reduction
T13314 5343-5345 IN denotes in
T13315 5346-5353 NN denotes binding
T13316 5354-5362 NN denotes activity
T13317 5363-5365 IN denotes to
T13318 5366-5369 DT denotes the
T13319 5398-5405 NN denotes protein
T13320 5370-5375 JJ denotes other
T13321 5376-5379 NN denotes SAM
T13322 5380-5386 NN denotes domain
T13323 5387-5397 VBG denotes containing
T13324 5386-5387 HYPH denotes -
T13325 5405-5407 , denotes ,
T13326 5407-5410 NN denotes Sex
T13327 5411-5415 NN denotes comb
T13328 5416-5418 IN denotes on
T13329 5419-5425 NN denotes midleg
T13330 5426-5427 -LRB- denotes (
T13331 5427-5430 NN denotes Scm
T13332 5430-5431 -RRB- denotes )
T13333 5432-5433 -LRB- denotes (
T13334 5433-5435 CD denotes 41
T13335 5435-5436 -RRB- denotes )
T13336 5436-5437 . denotes .
T13337 5437-5634 sentence denotes Based on this result, we introduced two types of site-directed mutations, which correspond to the mutations introduced in ph protein, into Flag-tagged full-length mr-s (Flag-W404A and Flag-G453A).
T13338 5438-5443 VBN denotes Based
T13339 5463-5473 VBD denotes introduced
T13340 5444-5446 IN denotes on
T13341 5447-5451 DT denotes this
T13342 5452-5458 NN denotes result
T13343 5458-5460 , denotes ,
T13344 5460-5462 PRP denotes we
T13345 5474-5477 CD denotes two
T13346 5478-5483 NNS denotes types
T13347 5484-5486 IN denotes of
T13348 5487-5491 NN denotes site
T13349 5492-5500 VBN denotes directed
T13350 5491-5492 HYPH denotes -
T13351 5501-5510 NNS denotes mutations
T13352 5510-5512 , denotes ,
T13353 5512-5517 WDT denotes which
T13354 5518-5528 VBP denotes correspond
T13355 5529-5531 IN denotes to
T13356 5532-5535 DT denotes the
T13357 5536-5545 NNS denotes mutations
T13358 5546-5556 VBN denotes introduced
T13359 5557-5559 IN denotes in
T13360 5560-5562 NN denotes ph
T13361 5563-5570 NN denotes protein
T13362 5570-5572 , denotes ,
T13363 5572-5576 IN denotes into
T13364 5577-5581 NN denotes Flag
T13365 5582-5588 VBN denotes tagged
T13366 5581-5582 HYPH denotes -
T13367 5604-5605 NN denotes s
T13368 5589-5593 JJ denotes full
T13369 5594-5600 NN denotes length
T13370 5593-5594 HYPH denotes -
T13371 5601-5603 NN denotes mr
T13372 5603-5604 HYPH denotes -
T13373 5606-5607 -LRB- denotes (
T13374 5607-5611 NN denotes Flag
T13375 5612-5617 NN denotes W404A
T13376 5611-5612 HYPH denotes -
T13377 5618-5621 CC denotes and
T13378 5622-5626 NN denotes Flag
T13379 5627-5632 NN denotes G453A
T13380 5626-5627 HYPH denotes -
T13381 5632-5633 -RRB- denotes )
T13382 5633-5634 . denotes .
T13383 5634-5792 sentence denotes We found that Flag-W404A binding activity was significantly reduced and Flag-G453A binding activity was also slightly reduced compared to Flag-mrs (Fig. 5C).
T13384 5635-5637 PRP denotes We
T13385 5638-5643 VBD denotes found
T13386 5644-5648 IN denotes that
T13387 5695-5702 VBN denotes reduced
T13388 5649-5653 NN denotes Flag
T13389 5654-5659 NN denotes W404A
T13390 5653-5654 HYPH denotes -
T13391 5660-5667 NN denotes binding
T13392 5668-5676 NN denotes activity
T13393 5677-5680 VBD denotes was
T13394 5681-5694 RB denotes significantly
T13395 5703-5706 CC denotes and
T13396 5707-5711 NN denotes Flag
T13397 5712-5717 NN denotes G453A
T13398 5711-5712 HYPH denotes -
T13399 5718-5725 NN denotes binding
T13400 5726-5734 NN denotes activity
T13401 5753-5760 VBN denotes reduced
T13402 5735-5738 VBD denotes was
T13403 5739-5743 RB denotes also
T13404 5744-5752 RB denotes slightly
T13405 5761-5769 VBN denotes compared
T13406 5770-5772 IN denotes to
T13407 5773-5777 NN denotes Flag
T13408 5778-5781 NN denotes mrs
T13409 5777-5778 HYPH denotes -
T13410 5782-5783 -LRB- denotes (
T13411 5788-5790 NN denotes 5C
T13412 5783-5787 NN denotes Fig.
T13413 5790-5791 -RRB- denotes )
T13414 5791-5792 . denotes .
T13415 5792-5991 sentence denotes These results, together with yeast two-hybrid GAL4 assay, indicate that the mr-s protein self-associates strongly through its SAM domain and weakly through the N-terminus portion lacking SAM domain.
T13416 5793-5798 DT denotes These
T13417 5799-5806 NNS denotes results
T13418 5851-5859 VBP denotes indicate
T13419 5806-5808 , denotes ,
T13420 5808-5816 RB denotes together
T13421 5817-5821 IN denotes with
T13422 5822-5827 NN denotes yeast
T13423 5844-5849 NN denotes assay
T13424 5828-5831 CD denotes two
T13425 5832-5838 NN denotes hybrid
T13426 5831-5832 HYPH denotes -
T13427 5839-5843 NN denotes GAL4
T13428 5849-5851 , denotes ,
T13429 5860-5864 IN denotes that
T13430 5887-5897 VBZ denotes associates
T13431 5865-5868 DT denotes the
T13432 5874-5881 NN denotes protein
T13433 5869-5871 NN denotes mr
T13434 5872-5873 NN denotes s
T13435 5871-5872 HYPH denotes -
T13436 5882-5886 NN denotes self
T13437 5886-5887 HYPH denotes -
T13438 5898-5906 RB denotes strongly
T13439 5907-5914 IN denotes through
T13440 5915-5918 PRP$ denotes its
T13441 5923-5929 NN denotes domain
T13442 5919-5922 NN denotes SAM
T13443 5930-5933 CC denotes and
T13444 5934-5940 RB denotes weakly
T13445 5941-5948 IN denotes through
T13446 5949-5952 DT denotes the
T13447 5964-5971 NN denotes portion
T13448 5953-5954 NN denotes N
T13449 5955-5963 NN denotes terminus
T13450 5954-5955 HYPH denotes -
T13451 5972-5979 VBG denotes lacking
T13452 5980-5983 NN denotes SAM
T13453 5984-5990 NN denotes domain
T13454 5990-5991 . denotes .
T30892 2473-2480 NN denotes Summary
T30893 2481-2483 IN denotes of
T30894 2484-2489 NN denotes yeast
T30895 2501-2510 NN denotes screening
T30896 2490-2493 CD denotes two
T30897 2494-2500 NN denotes hybrid
T30898 2493-2494 HYPH denotes -
T30899 2511-2514 CC denotes and
T30900 2515-2519 NN denotes GAL4
T30901 2520-2525 NN denotes assay
T30902 2525-2526 . denotes .
T30903 2526-2631 sentence denotes (A) Full-length mr-s as a bait used in the yeast two-hybrid screening and positive clones are indicated.
T30904 2527-2528 -LRB- denotes (
T30905 2528-2529 LS denotes A
T30906 2621-2630 VBN denotes indicated
T30907 2529-2530 -RRB- denotes )
T30908 2531-2535 JJ denotes Full
T30909 2536-2542 NN denotes length
T30910 2535-2536 HYPH denotes -
T30911 2546-2547 NN denotes s
T30912 2543-2545 NN denotes mr
T30913 2545-2546 HYPH denotes -
T30914 2548-2550 IN denotes as
T30915 2551-2552 DT denotes a
T30916 2553-2557 NN denotes bait
T30917 2558-2562 VBN denotes used
T30918 2563-2565 IN denotes in
T30919 2566-2569 DT denotes the
T30920 2587-2596 NN denotes screening
T30921 2570-2575 NN denotes yeast
T30922 2576-2579 CD denotes two
T30923 2580-2586 NN denotes hybrid
T30924 2579-2580 HYPH denotes -
T30925 2597-2600 CC denotes and
T30926 2601-2609 JJ denotes positive
T30927 2610-2616 NNS denotes clones
T30928 2617-2620 VBP denotes are
T30929 2630-2631 . denotes .
T30930 2631-2714 sentence denotes Note that all of five mr-s clones identified in the screening contain SAM domains.
T30931 2632-2636 VB denotes Note
T30932 2637-2641 IN denotes that
T30933 2694-2701 VBP denotes contain
T30934 2642-2645 DT denotes all
T30935 2646-2648 IN denotes of
T30936 2649-2653 CD denotes five
T30937 2659-2665 NNS denotes clones
T30938 2654-2656 NN denotes mr
T30939 2657-2658 NN denotes s
T30940 2656-2657 HYPH denotes -
T30941 2666-2676 VBN denotes identified
T30942 2677-2679 IN denotes in
T30943 2680-2683 DT denotes the
T30944 2684-2693 NN denotes screening
T30945 2702-2705 NN denotes SAM
T30946 2706-2713 NNS denotes domains
T30947 2713-2714 . denotes .
T30948 2714-2794 sentence denotes (B) Schematic diagram of the mr-s fusion proteins used in the yeast GAL4 assay.
T30949 2715-2716 -LRB- denotes (
T30950 2716-2717 LS denotes B
T30951 2729-2736 NN denotes diagram
T30952 2717-2718 -RRB- denotes )
T30953 2719-2728 JJ denotes Schematic
T30954 2737-2739 IN denotes of
T30955 2740-2743 DT denotes the
T30956 2756-2764 NN denotes proteins
T30957 2744-2746 NN denotes mr
T30958 2747-2748 NN denotes s
T30959 2746-2747 HYPH denotes -
T30960 2749-2755 NN denotes fusion
T30961 2765-2769 VBN denotes used
T30962 2770-2772 IN denotes in
T30963 2773-2776 DT denotes the
T30964 2788-2793 NN denotes assay
T30965 2777-2782 NN denotes yeast
T30966 2783-2787 NN denotes GAL4
T30967 2793-2794 . denotes .
T30968 2794-2845 sentence denotes Black boxes represent the position of SAM domains.
T30969 2795-2800 JJ denotes Black
T30970 2801-2806 NNS denotes boxes
T30971 2807-2816 VBP denotes represent
T30972 2817-2820 DT denotes the
T30973 2821-2829 NN denotes position
T30974 2830-2832 IN denotes of
T30975 2833-2836 NN denotes SAM
T30976 2837-2844 NNS denotes domains
T30977 2844-2845 . denotes .
T30978 2845-2918 sentence denotes Relative levels of LacZ expression are shown on the right, respectively.
T30979 2846-2854 JJ denotes Relative
T30980 2855-2861 NNS denotes levels
T30981 2885-2890 VBN denotes shown
T30982 2862-2864 IN denotes of
T30983 2865-2869 NN denotes LacZ
T30984 2870-2880 NN denotes expression
T30985 2881-2884 VBP denotes are
T30986 2891-2893 IN denotes on
T30987 2894-2897 DT denotes the
T30988 2898-2903 NN denotes right
T30989 2903-2905 , denotes ,
T30990 2905-2917 RB denotes respectively
T30991 2917-2918 . denotes .
T30992 2918-3062 sentence denotes Note; ++ indicates an intense blue signal visible after 12hr of incubation at 37°C, + indicates a blue signal visible after 24hr of incubation.
T30993 2919-2923 NN denotes Note
T30994 3005-3014 VBZ denotes indicates
T30995 2923-2924 : denotes ;
T30996 2925-2927 SYM denotes ++
T30997 2928-2937 VBZ denotes indicates
T30998 2938-2940 DT denotes an
T30999 2954-2960 NN denotes signal
T31000 2941-2948 JJ denotes intense
T31001 2949-2953 JJ denotes blue
T31002 2961-2968 JJ denotes visible
T31003 2969-2974 IN denotes after
T31004 2975-2979 NN denotes 12hr
T31005 2980-2982 IN denotes of
T31006 2983-2993 NN denotes incubation
T31007 2994-2996 IN denotes at
T31008 2997-2999 CD denotes 37
T31009 2999-3001 NN denotes °C
T31010 3001-3003 , denotes ,
T31011 3003-3004 SYM denotes +
T31012 3015-3016 DT denotes a
T31013 3022-3028 NN denotes signal
T31014 3017-3021 JJ denotes blue
T31015 3029-3036 JJ denotes visible
T31016 3037-3042 IN denotes after
T31017 3043-3047 NN denotes 24hr
T31018 3048-3050 IN denotes of
T31019 3051-3061 NN denotes incubation
T31020 3061-3062 . denotes .
T31021 3062-3239 sentence denotes BD, binding domain; AD, activation domain; full, full-length mr-s; N, N-terminal portion of mr-s (amino acids 1 to 400); C, C-terminal portion of mr-s (amino acids 391 to 542).
T31022 3063-3065 NN denotes BD
T31023 3065-3067 , denotes ,
T31024 3067-3074 NN denotes binding
T31025 3075-3081 NN denotes domain
T31026 3081-3082 : denotes ;
T31027 3083-3085 NN denotes AD
T31028 3085-3087 , denotes ,
T31029 3087-3097 NN denotes activation
T31030 3098-3104 NN denotes domain
T31031 3104-3105 : denotes ;
T31032 3106-3110 JJ denotes full
T31033 3127-3128 NN denotes s
T31034 3110-3112 , denotes ,
T31035 3112-3116 JJ denotes full
T31036 3117-3123 NN denotes length
T31037 3116-3117 HYPH denotes -
T31038 3124-3126 NN denotes mr
T31039 3126-3127 HYPH denotes -
T31040 3128-3129 : denotes ;
T31041 3130-3131 NN denotes N
T31042 3131-3133 , denotes ,
T31043 3133-3134 NN denotes N
T31044 3135-3143 JJ denotes terminal
T31045 3134-3135 HYPH denotes -
T31046 3144-3151 NN denotes portion
T31047 3152-3154 IN denotes of
T31048 3155-3157 NN denotes mr
T31049 3158-3159 NN denotes s
T31050 3157-3158 HYPH denotes -
T31051 3160-3161 -LRB- denotes (
T31052 3173-3174 CD denotes 1
T31053 3161-3166 NN denotes amino
T31054 3167-3172 NNS denotes acids
T31055 3175-3177 IN denotes to
T31056 3178-3181 CD denotes 400
T31057 3181-3182 -RRB- denotes )
T31058 3182-3183 : denotes ;
T31059 3184-3185 NN denotes C
T31060 3185-3187 , denotes ,
T31061 3187-3188 NN denotes C
T31062 3189-3197 JJ denotes terminal
T31063 3188-3189 HYPH denotes -
T31064 3198-3205 NN denotes portion
T31065 3206-3208 IN denotes of
T31066 3209-3211 NN denotes mr
T31067 3212-3213 NN denotes s
T31068 3211-3212 HYPH denotes -
T31069 3214-3215 -LRB- denotes (
T31070 3227-3230 CD denotes 391
T31071 3215-3220 NN denotes amino
T31072 3221-3226 NNS denotes acids
T31073 3231-3233 IN denotes to
T31074 3234-3237 CD denotes 542
T31075 3237-3238 -RRB- denotes )
T31076 3238-3239 . denotes .
T31407 4264-4267 DT denotes The
T31408 4273-4280 NN denotes protein
T31409 4268-4270 NN denotes mr
T31410 4271-4272 NN denotes s
T31411 4270-4271 HYPH denotes -
T31412 4290-4299 VB denotes associate
T31413 4281-4284 MD denotes can
T31414 4285-4289 NN denotes self
T31415 4289-4290 HYPH denotes -
T31416 4300-4302 IN denotes in
T31417 4303-4312 JJ denotes mammalian
T31418 4313-4318 NNS denotes cells
T31419 4318-4319 . denotes .
T31420 4319-4395 sentence denotes (A) Schematic drawing of the constructs used for immunoprecipitation assay.
T31421 4320-4321 -LRB- denotes (
T31422 4321-4322 LS denotes A
T31423 4334-4341 NN denotes drawing
T31424 4322-4323 -RRB- denotes )
T31425 4324-4333 JJ denotes Schematic
T31426 4342-4344 IN denotes of
T31427 4345-4348 DT denotes the
T31428 4349-4359 NNS denotes constructs
T31429 4360-4364 VBN denotes used
T31430 4365-4368 IN denotes for
T31431 4369-4388 NN denotes immunoprecipitation
T31432 4389-4394 NN denotes assay
T31433 4394-4395 . denotes .
T31434 4395-4573 sentence denotes HA-tagged or Flag-tagged full-length (amino acids 1 to 542), ΔSAM (amino acids 1 to 400) and SAM (amino acids 401 to 542) regions were inserted into pcDNA3 vector, respectively.
T31435 4396-4398 NN denotes HA
T31436 4399-4405 VBN denotes tagged
T31437 4398-4399 HYPH denotes -
T31438 4518-4525 NNS denotes regions
T31439 4406-4408 CC denotes or
T31440 4409-4413 NN denotes Flag
T31441 4414-4420 VBN denotes tagged
T31442 4413-4414 HYPH denotes -
T31443 4421-4425 JJ denotes full
T31444 4426-4432 NN denotes length
T31445 4425-4426 HYPH denotes -
T31446 4433-4434 -LRB- denotes (
T31447 4446-4447 CD denotes 1
T31448 4434-4439 NN denotes amino
T31449 4440-4445 NNS denotes acids
T31450 4448-4450 IN denotes to
T31451 4451-4454 CD denotes 542
T31452 4454-4455 -RRB- denotes )
T31453 4455-4457 , denotes ,
T31454 4457-4461 NN denotes ΔSAM
T31455 4462-4463 -LRB- denotes (
T31456 4475-4476 CD denotes 1
T31457 4463-4468 NN denotes amino
T31458 4469-4474 NNS denotes acids
T31459 4477-4479 IN denotes to
T31460 4480-4483 CD denotes 400
T31461 4483-4484 -RRB- denotes )
T31462 4485-4488 CC denotes and
T31463 4489-4492 NN denotes SAM
T31464 4493-4494 -LRB- denotes (
T31465 4506-4509 CD denotes 401
T31466 4494-4499 NN denotes amino
T31467 4500-4505 NNS denotes acids
T31468 4510-4512 IN denotes to
T31469 4513-4516 CD denotes 542
T31470 4516-4517 -RRB- denotes )
T31471 4531-4539 VBN denotes inserted
T31472 4526-4530 VBD denotes were
T31473 4540-4544 IN denotes into
T31474 4545-4551 NN denotes pcDNA3
T31475 4552-4558 NN denotes vector
T31476 4558-4560 , denotes ,
T31477 4560-4572 RB denotes respectively
T31478 4572-4573 . denotes .
T31479 4573-4645 sentence denotes (B) The constructs indicated above were transfected into HEK293T cells.
T31480 4574-4575 -LRB- denotes (
T31481 4575-4576 LS denotes B
T31482 4614-4625 VBN denotes transfected
T31483 4576-4577 -RRB- denotes )
T31484 4578-4581 DT denotes The
T31485 4582-4592 NNS denotes constructs
T31486 4593-4602 VBN denotes indicated
T31487 4603-4608 RB denotes above
T31488 4609-4613 VBD denotes were
T31489 4626-4630 IN denotes into
T31490 4631-4638 NN denotes HEK293T
T31491 4639-4644 NNS denotes cells
T31492 4644-4645 . denotes .
T31493 4645-4737 sentence denotes Each lane was co-immunoprecipitated by anti-Flag antibody and detected by anti-HA antibody.
T31494 4646-4650 DT denotes Each
T31495 4651-4655 NN denotes lane
T31496 4660-4681 VBN denotes co-immunoprecipitated
T31497 4656-4659 VBD denotes was
T31498 4682-4684 IN denotes by
T31499 4685-4694 JJ denotes anti-Flag
T31500 4695-4703 NN denotes antibody
T31501 4704-4707 CC denotes and
T31502 4708-4716 VBN denotes detected
T31503 4717-4719 IN denotes by
T31504 4720-4727 JJ denotes anti-HA
T31505 4728-4736 NN denotes antibody
T31506 4736-4737 . denotes .
T31507 4737-4790 sentence denotes Input protein lysates are shown in the lower panels.
T31508 4738-4743 NN denotes Input
T31509 4752-4759 NNS denotes lysates
T31510 4744-4751 NN denotes protein
T31511 4764-4769 VBN denotes shown
T31512 4760-4763 VBP denotes are
T31513 4770-4772 IN denotes in
T31514 4773-4776 DT denotes the
T31515 4783-4789 NNS denotes panels
T31516 4777-4782 JJ denotes lower
T31517 4789-4790 . denotes .
T31518 4790-4912 sentence denotes (C) Flag-tagged two site-directed mr-s mutants, Flag-W404A and Flag-G453A were generated and co-transfected with full-HA.
T31519 4791-4792 -LRB- denotes (
T31520 4792-4793 LS denotes C
T31521 4870-4879 VBN denotes generated
T31522 4793-4794 -RRB- denotes )
T31523 4795-4799 NN denotes Flag
T31524 4800-4806 VBN denotes tagged
T31525 4799-4800 HYPH denotes -
T31526 4830-4837 NNS denotes mutants
T31527 4807-4810 CD denotes two
T31528 4811-4815 NN denotes site
T31529 4816-4824 VBN denotes directed
T31530 4815-4816 HYPH denotes -
T31531 4825-4827 NN denotes mr
T31532 4828-4829 NN denotes s
T31533 4827-4828 HYPH denotes -
T31534 4837-4839 , denotes ,
T31535 4839-4843 NN denotes Flag
T31536 4844-4849 NN denotes W404A
T31537 4843-4844 HYPH denotes -
T31538 4850-4853 CC denotes and
T31539 4854-4858 NN denotes Flag
T31540 4859-4864 NN denotes G453A
T31541 4858-4859 HYPH denotes -
T31542 4865-4869 VBD denotes were
T31543 4880-4883 CC denotes and
T31544 4884-4898 VBN denotes co-transfected
T31545 4899-4903 IN denotes with
T31546 4904-4908 JJ denotes full
T31547 4909-4911 NN denotes HA
T31548 4908-4909 HYPH denotes -
T31549 4911-4912 . denotes .
T31550 4912-5004 sentence denotes Each lane was co-immunoprecipitated by anti-HA antibody and detected by anti-Flag antibody.
T31551 4913-4917 DT denotes Each
T31552 4918-4922 NN denotes lane
T31553 4927-4948 VBN denotes co-immunoprecipitated
T31554 4923-4926 VBD denotes was
T31555 4949-4951 IN denotes by
T31556 4952-4959 JJ denotes anti-HA
T31557 4960-4968 NN denotes antibody
T31558 4969-4972 CC denotes and
T31559 4973-4981 VBN denotes detected
T31560 4982-4984 IN denotes by
T31561 4985-4994 JJ denotes anti-Flag
T31562 4995-5003 NN denotes antibody
T31563 5003-5004 . denotes .
T13281 5162-5166 NN denotes Fig.
T13282 5167-5169 NN denotes 1B
T12794 1555-1559 VBZ denotes does
T13283 5169-5171 , denotes ,
T12899 2007-2008 HYPH denotes -
T12900 2010-2011 -LRB- denotes (
T12901 2027-2028 NN denotes C
T12902 2011-2015 NN denotes Fig.
T12903 2016-2018 NN denotes 4B
T12904 2018-2020 , denotes ,
T12998 3240-3486 sentence denotes To confirm self-association of the mr-s protein in mammalian cells, we next performed co-immunoprecipitation studies in HEK293T cells by co-transfection of HA-tagged full-length/truncated mr-s and Flag-tagged full-length/truncated mr-s (Fig. 5A).
T12999 3240-3242 TO denotes To
T13000 3243-3250 VB denotes confirm
T13001 3316-3325 VBD denotes performed
T13002 3251-3255 NN denotes self
T13003 3256-3267 NN denotes association
T13004 3255-3256 HYPH denotes -
T13005 3268-3270 IN denotes of
T13006 3271-3274 DT denotes the
T13007 3280-3287 NN denotes protein
T13008 3275-3277 NN denotes mr
T13009 3278-3279 NN denotes s
T13010 3277-3278 HYPH denotes -
T13011 3288-3290 IN denotes in
T13012 3291-3300 JJ denotes mammalian
T13013 3301-3306 NNS denotes cells
T13014 3306-3308 , denotes ,
T13015 3308-3310 PRP denotes we
T13016 3311-3315 RB denotes next
T13017 3326-3348 NN denotes co-immunoprecipitation
T13018 3349-3356 NNS denotes studies
T13019 3357-3359 IN denotes in
T13020 3360-3367 NN denotes HEK293T
T13021 3368-3373 NNS denotes cells
T13022 3374-3376 IN denotes by
T13023 3377-3392 NN denotes co-transfection
T13024 3393-3395 IN denotes of
T13025 3396-3398 NN denotes HA
T13026 3399-3405 VBN denotes tagged
T13236 4230-4233 NN denotes SAM
T13237 4229-4230 HYPH denotes -
T13238 4234-4235 -LRB- denotes (
T13239 4240-4242 NN denotes 5B
T13240 4235-4239 NN denotes Fig.
T13241 4242-4244 , denotes ,
T13242 4244-4248 NN denotes lane
T13243 4249-4251 CD denotes 10
T13244 4251-4252 -RRB- denotes )
T13245 4252-4253 . denotes .
T13246 4253-5179 sentence denotes Figure 5 The mr-s protein can self-associate in mammalian cells. (A) Schematic drawing of the constructs used for immunoprecipitation assay. HA-tagged or Flag-tagged full-length (amino acids 1 to 542), ΔSAM (amino acids 1 to 400) and SAM (amino acids 401 to 542) regions were inserted into pcDNA3 vector, respectively. (B) The constructs indicated above were transfected into HEK293T cells. Each lane was co-immunoprecipitated by anti-Flag antibody and detected by anti-HA antibody. Input protein lysates are shown in the lower panels. (C) Flag-tagged two site-directed mr-s mutants, Flag-W404A and Flag-G453A were generated and co-transfected with full-HA. Each lane was co-immunoprecipitated by anti-HA antibody and detected by anti-Flag antibody. To investigate whether the mr-s protein self-associates mainly through the SAM domain, two site-directed mutations were generated in the SAM domain of mr-s (Fig. 1B, arrows).
T13247 5005-5007 TO denotes To
T13248 5008-5019 VB denotes investigate
T13249 5125-5134 VBN denotes generated
T13250 5020-5027 IN denotes whether
T13251 5050-5060 VBZ denotes associates
T13252 5028-5031 DT denotes the
T13253 5037-5044 NN denotes protein
T13254 5032-5034 NN denotes mr
T13255 5035-5036 NN denotes s
T13256 5034-5035 HYPH denotes -
T13257 5045-5049 NN denotes self
T13258 5049-5050 HYPH denotes -
T13259 5061-5067 RB denotes mainly
T13260 5068-5075 IN denotes through
T13261 5076-5079 DT denotes the
T13262 5084-5090 NN denotes domain
T13263 5080-5083 NN denotes SAM
T13264 5090-5092 , denotes ,
T13265 5092-5095 CD denotes two
T13266 5110-5119 NNS denotes mutations
T13267 5096-5100 NN denotes site
T13268 5101-5109 VBN denotes directed
T13269 5100-5101 HYPH denotes -
T13270 5120-5124 VBD denotes were
T13271 5135-5137 IN denotes in
T13272 5138-5141 DT denotes the
T13273 5146-5152 NN denotes domain
T13274 5142-5145 NN denotes SAM
T13275 5153-5155 IN denotes of
T13276 5156-5158 NN denotes mr
T13277 5159-5160 NN denotes s
T13278 5158-5159 HYPH denotes -
T13279 5161-5162 -LRB- denotes (
T13280 5171-5177 NNS denotes arrows
R2867 T12462 T12463 compound Self,association
R2868 T12464 T12463 punct -,association
R2869 T12465 T12463 prep of,association
R2870 T12466 T12467 compound mr,s
R2871 T12467 T12469 compound s,protein
R2872 T12468 T12467 punct -,s
R2873 T12469 T12465 pobj protein,of
R2874 T12471 T12472 compound SAM,domains
R2875 T12472 T12473 nsubjpass domains,known
R2876 T12474 T12473 auxpass are,known
R2877 T12475 T12476 aux to,function
R2878 T12476 T12473 xcomp function,known
R2879 T12477 T12476 prep as,function
R2880 T12478 T12479 compound protein,protein
R2881 T12479 T12481 compound protein,modules
R2882 T12480 T12479 punct -,protein
R2883 T12481 T12477 pobj modules,as
R2884 T12482 T12481 compound interaction,modules
R2885 T12483 T12484 punct [,15
R2886 T12484 T12473 parataxis 15,known
R2887 T12485 T12486 punct -,17
R2888 T12486 T12484 prep 17,15
R2889 T12487 T12484 punct ],15
R2890 T12488 T12473 punct .,known
R2891 T12490 T12491 mark Although,bind
R2892 T12491 T12495 advcl bind,reported
R2893 T12492 T12493 compound SAM,domains
R2894 T12493 T12491 nsubj domains,bind
R2895 T12494 T12491 aux can,bind
R2896 T12496 T12491 prep to,bind
R2897 T12497 T12498 amod various,proteins
R2898 T12498 T12496 pobj proteins,to
R2899 T12499 T12500 amod non-SAM,domain
R2900 T12500 T12501 npadvmod domain,containing
R2901 T12501 T12498 amod containing,proteins
R2902 T12502 T12501 punct -,containing
R2903 T12503 T12495 punct ", ",reported
R2904 T12504 T12505 amod many,interactions
R2905 T12505 T12495 nsubjpass interactions,reported
R2906 T12506 T12505 nmod homo-SAM,interactions
R2907 T12507 T12506 cc and,homo-SAM
R2908 T12508 T12506 conj hetero-SAM,homo-SAM
R2909 T12509 T12505 compound domain,interactions
R2910 T12510 T12495 aux have,reported
R2911 T12511 T12495 auxpass been,reported
R2912 T12512 T12495 punct .,reported
R2913 T12514 T12515 aux To,investigate
R2914 T12515 T12516 advcl investigate,performed
R2915 T12517 T12518 mark whether,function
R2916 T12518 T12515 ccomp function,investigate
R2917 T12519 T12520 det the,domain
R2918 T12520 T12518 nsubj domain,function
R2919 T12521 T12520 compound SAM,domain
R2920 T12522 T12520 prep of,domain
R2921 T12523 T12524 det the,protein
R2922 T12524 T12522 pobj protein,of
R2923 T12525 T12526 compound mr,s
R2924 T12526 T12524 compound s,protein
R2925 T12527 T12526 punct -,s
R2926 T12528 T12518 aux can,function
R2927 T12529 T12518 advmod also,function
R2928 T12530 T12518 prep as,function
R2929 T12531 T12532 det a,module
R2930 T12532 T12530 pobj module,as
R2931 T12533 T12534 compound protein,protein
R2932 T12534 T12532 compound protein,module
R2933 T12535 T12534 punct -,protein
R2934 T12536 T12532 compound interaction,module
R2935 T12537 T12516 punct ", ",performed
R2936 T12538 T12516 nsubj we,performed
R2937 T12539 T12540 nmod yeast,screening
R2938 T12540 T12516 dobj screening,performed
R2939 T12541 T12542 nummod two,hybrid
R2940 T12542 T12540 compound hybrid,screening
R2941 T12543 T12542 punct -,hybrid
R2942 T12544 T12516 advcl using,performed
R2943 T12545 T12546 amod full,length
R2944 T12546 T12548 compound length,protein
R2945 T12547 T12546 punct -,length
R2946 T12548 T12544 dobj protein,using
R2947 T12549 T12550 compound mr,s
R2948 T12550 T12548 compound s,protein
R2949 T12551 T12550 punct -,s
R2950 T12552 T12544 prep as,using
R2951 T12553 T12554 det the,bait
R2952 T12554 T12552 pobj bait,as
R2953 T12555 T12516 punct .,performed
R2954 T12557 T12558 advcl Using,screened
R2955 T12559 T12560 det this,bait
R2956 T12560 T12557 dobj bait,Using
R2957 T12561 T12558 punct ", ",screened
R2958 T12562 T12558 nsubj we,screened
R2959 T12563 T12564 det the,library
R2960 T12564 T12558 dobj library,screened
R2961 T12565 T12566 amod transcriptional,activator
R2962 T12566 T12567 compound activator,protein
R2963 T12567 T12564 compound protein,library
R2964 T12568 T12567 compound fusion,protein
R2965 T12569 T12570 prep in,fused
R2966 T12570 T12564 advcl fused,library
R2967 T12571 T12569 pobj which,in
R2968 T12572 T12573 nmod mouse,cDNAs
R2969 T12573 T12570 nsubjpass cDNAs,fused
R2970 T12574 T12573 nmod P0,cDNAs
R2971 T12575 T12576 punct -,P3
R2972 T12576 T12574 prep P3,P0
R2973 T12577 T12573 amod retinal,cDNAs
R2974 T12578 T12570 auxpass were,fused
R2975 T12579 T12570 prep to,fused
R2976 T12580 T12581 det the,domain
R2977 T12581 T12579 pobj domain,to
R2978 T12582 T12581 compound GAL4,domain
R2979 T12583 T12581 compound activation,domain
R2980 T12584 T12558 punct .,screened
R2981 T12586 T12587 det The,clones
R2982 T12587 T12591 nsubj clones,were
R2983 T12588 T12589 advmod most,frequent
R2984 T12589 T12587 amod frequent,clones
R2985 T12590 T12587 amod positive,clones
R2986 T12592 T12593 punct (,28
R2987 T12593 T12587 parataxis 28,clones
R2988 T12594 T12593 quantmod 5,28
R2989 T12595 T12593 quantmod out,28
R2990 T12596 T12593 quantmod of,28
R2991 T12597 T12593 punct ),28
R2992 T12598 T12599 compound cDNA,fragments
R2993 T12599 T12591 attr fragments,were
R2994 T12600 T12599 acl containing,fragments
R2995 T12601 T12602 det the,domain
R2996 T12602 T12600 dobj domain,containing
R2997 T12603 T12602 compound SAM,domain
R2998 T12604 T12602 prep of,domain
R2999 T12605 T12606 compound mr,s
R3000 T12606 T12604 pobj s,of
R3001 T12607 T12606 punct -,s
R3002 T12608 T12609 punct (,4A
R3003 T12609 T12591 parataxis 4A,were
R3004 T12610 T12609 compound Fig.,4A
R3005 T12611 T12609 punct ),4A
R3006 T12612 T12591 punct .,were
R3007 T12614 T12615 det This,result
R3008 T12615 T12616 nsubj result,suggests
R3009 T12617 T12616 advmod strongly,suggests
R3010 T12618 T12619 mark that,associates
R3011 T12619 T12616 ccomp associates,suggests
R3012 T12620 T12621 compound mr,s
R3013 T12621 T12623 compound s,protein
R3014 T12622 T12621 punct -,s
R3015 T12623 T12619 nsubj protein,associates
R3016 T12624 T12619 dep self,associates
R3017 T12625 T12619 punct -,associates
R3018 T12626 T12619 prep through,associates
R3019 T12627 T12628 compound SAM,domain
R3020 T12628 T12629 npadvmod domain,containing
R3021 T12629 T12631 amod containing,regions
R3022 T12630 T12629 punct -,containing
R3023 T12631 T12626 pobj regions,through
R3024 T12632 T12616 punct .,suggests
R3025 T12634 T12635 nsubj We,tested
R3026 T12636 T12635 advmod then,tested
R3027 T12637 T12635 advmod directly,tested
R3028 T12638 T12639 det this,association
R3029 T12639 T12635 dobj association,tested
R3030 T12640 T12639 compound self,association
R3031 T12641 T12639 punct -,association
R3032 T12642 T12639 prep of,association
R3033 T12643 T12644 compound mr,s
R3034 T12644 T12646 compound s,protein
R3035 T12645 T12644 punct -,s
R3036 T12646 T12642 pobj protein,of
R3037 T12647 T12635 prep in,tested
R3038 T12648 T12647 pobj yeast,in
R3039 T12649 T12635 punct .,tested
R3040 T12651 T12652 nsubj We,fused
R3041 T12653 T12654 amod full,length
R3042 T12654 T12656 nmod length,portions
R3043 T12655 T12654 punct -,length
R3044 T12656 T12652 dobj portions,fused
R3045 T12657 T12654 cc or,length
R3046 T12658 T12654 conj truncated,length
R3047 T12659 T12656 prep of,portions
R3048 T12660 T12661 det the,protein
R3049 T12661 T12659 pobj protein,of
R3050 T12662 T12663 compound mr,s
R3051 T12663 T12661 compound s,protein
R3052 T12664 T12663 punct -,s
R3053 T12665 T12652 prep to,fused
R3054 T12666 T12667 det the,domain
R3055 T12667 T12665 pobj domain,to
R3056 T12668 T12669 npadvmod DNA,binding
R3057 T12669 T12667 amod binding,domain
R3058 T12670 T12669 punct -,binding
R3059 T12671 T12667 prep of,domain
R3060 T12672 T12673 det the,factor
R3061 T12673 T12671 pobj factor,of
R3062 T12674 T12673 compound yeast,factor
R3063 T12675 T12673 compound transcription,factor
R3064 T12676 T12673 appos GAL4,factor
R3065 T12677 T12678 aux to,make
R3066 T12678 T12652 advcl make,fused
R3067 T12679 T12680 compound bait,constructs
R3068 T12680 T12678 dobj constructs,make
R3069 T12681 T12652 punct .,fused
R3070 T12683 T12684 nsubj We,fused
R3071 T12685 T12686 amod full,length
R3072 T12686 T12688 nmod length,portions
R3073 T12687 T12686 punct -,length
R3074 T12688 T12684 dobj portions,fused
R3075 T12689 T12686 cc or,length
R3076 T12690 T12686 conj truncated,length
R3077 T12691 T12688 prep of,portions
R3078 T12692 T12693 det the,protein
R3079 T12693 T12691 pobj protein,of
R3080 T12694 T12695 compound mr,s
R3081 T12695 T12693 compound s,protein
R3082 T12696 T12695 punct -,s
R3083 T12697 T12684 prep to,fused
R3084 T12698 T12699 det the,domain
R3085 T12699 T12697 pobj domain,to
R3086 T12700 T12701 nmod GAL4,activation
R3087 T12701 T12699 compound activation,domain
R3088 T12702 T12701 amod transcriptional,activation
R3089 T12703 T12704 aux to,make
R3090 T12704 T12684 advcl make,fused
R3091 T12705 T12706 compound prey,constructs
R3092 T12706 T12704 dobj constructs,make
R3093 T12707 T12708 punct (,4B
R3094 T12708 T12684 parataxis 4B,fused
R3095 T12709 T12708 compound Fig.,4B
R3096 T12710 T12708 punct ),4B
R3097 T12711 T12684 punct .,fused
R3098 T12713 T12714 det These,constructs
R3099 T12714 T12715 nsubjpass constructs,transformed
R3100 T12716 T12715 auxpass were,transformed
R3101 T12717 T12715 prep into,transformed
R3102 T12718 T12717 pobj yeast,into
R3103 T12719 T12720 dep that,contain
R3104 T12720 T12718 relcl contain,yeast
R3105 T12721 T12722 det a,transgene
R3106 T12722 T12720 dobj transgene,contain
R3107 T12723 T12722 prep with,transgene
R3108 T12724 T12725 compound GAL4,binding
R3109 T12725 T12726 compound binding,sites
R3110 T12726 T12723 pobj sites,with
R3111 T12727 T12726 advmod upstream,sites
R3112 T12728 T12727 prep of,upstream
R3113 T12729 T12730 det the,gene
R3114 T12730 T12728 pobj gene,of
R3115 T12731 T12730 compound lacZ,gene
R3116 T12732 T12715 punct .,transformed
R3117 T12734 T12735 nsubj We,found
R3118 T12736 T12737 mark that,induced
R3119 T12737 T12735 ccomp induced,found
R3120 T12738 T12739 det the,construct
R3121 T12739 T12737 nsubj construct,induced
R3122 T12740 T12741 amod full,length
R3123 T12741 T12739 compound length,construct
R3124 T12742 T12741 punct -,length
R3125 T12743 T12744 compound mr,s
R3126 T12744 T12739 compound s,construct
R3127 T12745 T12744 punct -,s
R3128 T12746 T12739 compound bait,construct
R3129 T12747 T12748 compound lacZ,expression
R3130 T12748 T12737 dobj expression,induced
R3131 T12749 T12737 prep with,induced
R3132 T12750 T12751 det the,construct
R3133 T12751 T12749 pobj construct,with
R3134 T12752 T12753 amod full,length
R3135 T12753 T12751 compound length,construct
R3136 T12754 T12753 punct -,length
R3137 T12755 T12756 compound mr,s
R3138 T12756 T12751 compound s,construct
R3139 T12757 T12756 punct -,s
R3140 T12758 T12751 compound prey,construct
R3141 T12759 T12760 punct (,full
R3142 T12760 T12735 parataxis full,found
R3143 T12761 T12762 compound Fig.,4B
R3144 T12762 T12760 dep 4B,full
R3145 T12763 T12760 punct ", ",full
R3146 T12764 T12760 amod full,full
R3147 T12765 T12760 punct ×,full
R3148 T12766 T12760 punct ),full
R3149 T12767 T12735 punct .,found
R3150 T12769 T12770 det The,stretch
R3151 T12770 T12780 nsubj stretch,activate
R3152 T12771 T12772 nmod N,terminus
R3153 T12772 T12770 nmod terminus,stretch
R3154 T12773 T12772 punct -,terminus
R3155 T12774 T12770 nummod 400,stretch
R3156 T12775 T12776 nmod amino,acid
R3157 T12776 T12770 nmod acid,stretch
R3158 T12777 T12776 punct (,acid
R3159 T12778 T12776 appos aa,acid
R3160 T12779 T12770 punct ),stretch
R3161 T12781 T12770 prep of,stretch
R3162 T12782 T12783 compound mr,s
R3163 T12783 T12781 pobj s,of
R3164 T12784 T12783 punct -,s
R3165 T12785 T12770 punct ", ",stretch
R3166 T12786 T12787 dep which,contain
R3167 T12787 T12770 relcl contain,stretch
R3168 T12788 T12787 aux does,contain
R3169 T12789 T12787 neg not,contain
R3170 T12790 T12791 det a,domain
R3171 T12791 T12787 dobj domain,contain
R3172 T12792 T12791 compound SAM,domain
R3173 T12793 T12780 punct ", ",activate
R3174 T12794 T12780 aux does,activate
R3175 T12795 T12780 advmod weakly,activate
R3176 T12796 T12780 dobj transcription,activate
R3177 T12797 T12796 prep of,transcription
R3178 T12798 T12797 pobj lacZ,of
R3179 T12799 T12800 punct (,N
R3180 T12800 T12780 parataxis N,activate
R3181 T12801 T12802 compound Fig.,4B
R3182 T12802 T12800 dep 4B,N
R3183 T12803 T12800 punct ", ",N
R3184 T12804 T12800 amod full,N
R3185 T12805 T12800 punct ×,N
R3186 T12806 T12800 punct ),N
R3187 T12807 T12780 punct .,activate
R3188 T12809 T12810 det The,stretch
R3189 T12810 T12816 nsubj stretch,was
R3190 T12811 T12812 nmod N,terminus
R3191 T12812 T12810 nmod terminus,stretch
R3192 T12813 T12812 punct -,terminus
R3193 T12814 T12815 nummod 400,aa
R3194 T12815 T12810 compound aa,stretch
R3195 T12817 T12810 prep of,stretch
R3196 T12818 T12819 compound mr,s
R3197 T12819 T12817 pobj s,of
R3198 T12820 T12819 punct -,s
R3199 T12821 T12816 acomp able,was
R3200 T12822 T12823 aux to,induce
R3201 T12823 T12821 xcomp induce,able
R3202 T12824 T12823 dobj transcription,induce
R3203 T12825 T12824 prep of,transcription
R3204 T12826 T12825 pobj lacZ,of
R3205 T12827 T12823 advmod weakly,induce
R3206 T12828 T12823 prep with,induce
R3207 T12829 T12830 det the,stretch
R3208 T12830 T12828 pobj stretch,with
R3209 T12831 T12830 amod same,stretch
R3210 T12832 T12833 nmod N,terminus
R3211 T12833 T12830 nmod terminus,stretch
R3212 T12834 T12833 punct -,terminus
R3213 T12835 T12836 nummod 400,aa
R3214 T12836 T12830 compound aa,stretch
R3215 T12837 T12830 prep of,stretch
R3216 T12838 T12839 compound mr,s
R3217 T12839 T12837 pobj s,of
R3218 T12840 T12839 punct -,s
R3219 T12841 T12842 punct (,N
R3220 T12842 T12816 parataxis N,was
R3221 T12843 T12844 compound Fig.,4B
R3222 T12844 T12842 dep 4B,N
R3223 T12845 T12842 punct ", ",N
R3224 T12846 T12842 nmod N,N
R3225 T12847 T12842 punct ×,N
R3226 T12848 T12842 punct ),N
R3227 T12849 T12816 punct .,was
R3228 T12851 T12852 mark Although,activates
R3229 T12852 T12864 advcl activates,observed
R3230 T12853 T12854 det the,protein
R3231 T12854 T12852 nsubj protein,activates
R3232 T12855 T12856 nmod N,terminus
R3233 T12856 T12854 nmod terminus,protein
R3234 T12857 T12856 punct -,terminus
R3235 T12858 T12859 nummod 400,aa
R3236 T12859 T12854 compound aa,protein
R3237 T12860 T12861 compound mr,s
R3238 T12861 T12854 compound s,protein
R3239 T12862 T12861 punct -,s
R3240 T12863 T12852 advmod weakly,activates
R3241 T12865 T12866 compound lacZ,transcription
R3242 T12866 T12852 dobj transcription,activates
R3243 T12867 T12852 prep with,activates
R3244 T12868 T12869 det the,portion
R3245 T12869 T12867 pobj portion,with
R3246 T12870 T12869 amod same,portion
R3247 T12871 T12872 compound N,terminus
R3248 T12872 T12869 compound terminus,portion
R3249 T12873 T12872 punct -,terminus
R3250 T12874 T12864 punct ", ",observed
R3251 T12875 T12876 det a,activation
R3252 T12876 T12864 nsubjpass activation,observed
R3253 T12877 T12878 advmod much,stronger
R3254 T12878 T12876 amod stronger,activation
R3255 T12879 T12876 prep of,activation
R3256 T12880 T12881 compound lacZ,expression
R3257 T12881 T12879 pobj expression,of
R3258 T12882 T12864 auxpass was,observed
R3259 T12883 T12864 prep with,observed
R3260 T12884 T12885 det a,portion
R3261 T12885 T12883 pobj portion,with
R3262 T12886 T12887 compound C,terminus
R3263 T12887 T12885 compound terminus,portion
R3264 T12888 T12887 punct -,terminus
R3265 T12889 T12885 acl encoding,portion
R3266 T12890 T12891 det the,stretch
R3267 T12891 T12889 dobj stretch,encoding
R3268 T12892 T12893 nummod 391,aa
R3269 T12893 T12891 compound aa,stretch
R3270 T12894 T12895 punct –,542
R3271 T12895 T12892 prep 542,391
R3272 T12896 T12891 prep of,stretch
R3273 T12897 T12898 compound mr,s
R3274 T12898 T12896 pobj s,of
R3275 T12899 T12898 punct -,s
R3276 T12900 T12901 punct (,C
R3277 T12901 T12864 parataxis C,observed
R3278 T12902 T12903 compound Fig.,4B
R3279 T12903 T12901 dep 4B,C
R3280 T12904 T12901 punct ", ",C
R3281 T12905 T12901 amod full,C
R3282 T12906 T12901 punct ×,C
R3283 T12907 T12901 punct ", ",C
R3284 T12908 T12909 nmod C,C
R3285 T12909 T12901 appos C,C
R3286 T12910 T12909 punct ×,C
R3287 T12911 T12901 punct ),C
R3288 T12912 T12864 punct .,observed
R3289 T12914 T12915 poss Our,assay
R3290 T12915 T12917 nsubj assay,indicated
R3291 T12916 T12915 compound GAL4,assay
R3292 T12918 T12919 mark that,was
R3294 T12920 T12921 det the,signal
R3295 T12921 T12919 nsubj signal,was
R3296 T12922 T12923 advmod when,was
R3298 T12924 T12925 det the,s
R3299 T12925 T12923 nsubj s,was
R3300 T12926 T12927 amod full,length
R3301 T12927 T12925 compound length,s
R3302 T12928 T12927 punct -,length
R3303 T12929 T12925 compound mr,s
R3304 T12930 T12925 punct -,s
R3319 T12945 T12944 compound SAM,domains
R3320 T12946 T12942 auxpass were,used
R3321 T12947 T12917 punct .,indicated
R3322 T12949 T12950 nsubj This,reflect
R3323 T12951 T12950 aux may,reflect
R3324 T12952 T12950 advmod simply,reflect
R3325 T12953 T12954 det the,tendency
R3326 T12954 T12950 dobj tendency,reflect
R3327 T12955 T12956 mark for,enter
R3328 T12956 T12954 advcl enter,tendency
R3329 T12957 T12958 det the,proteins
R3330 T12958 T12956 nsubj proteins,enter
R3331 T12959 T12958 amod small,proteins
R3332 T12960 T12958 compound fusion,proteins
R3333 T12961 T12956 aux to,enter
R3334 T12962 T12963 det the,nucleus
R3335 T12963 T12956 dobj nucleus,enter
R3336 T12964 T12963 compound yeast,nucleus
R3337 T12965 T12956 cc and,enter
R3338 T12966 T12956 conj occupy,enter
R3339 T12967 T12968 compound GAL4,binding
R3340 T12968 T12969 compound binding,sites
R3341 T12969 T12966 dobj sites,occupy
R3342 T12970 T12950 punct .,reflect
R3343 T12972 T12973 advmod Alternatively,be
R3344 T12974 T12973 punct ", ",be
R3345 T12975 T12976 det the,domain
R3346 T12976 T12973 nsubj domain,be
R3347 T12977 T12976 compound SAM,domain
R3348 T12978 T12973 aux may,be
R3349 T12979 T12980 advmod less,accessible
R3350 T12980 T12973 acomp accessible,be
R3351 T12981 T12980 prep for,accessible
R3352 T12982 T12981 pobj interaction,for
R3353 T12983 T12973 prep in,be
R3354 T12984 T12985 det the,context
R3355 T12985 T12983 pobj context,in
R3356 T12986 T12987 amod full,length
R3357 T12987 T12985 compound length,context
R3358 T12988 T12987 punct -,length
R3359 T12989 T12985 compound protein,context
R3360 T12990 T12991 mark as,reported
R3361 T12991 T12973 advcl reported,be
R3362 T12992 T12991 advmod previously,reported
R3363 T12993 T12994 punct [,39
R3364 T12994 T12973 parataxis 39,be
R3365 T12995 T12994 punct ],39
R3366 T12996 T12973 punct .,be
R3372 T13005 T13003 prep of,association
R3373 T13006 T13007 det the,protein
R3374 T13007 T13005 pobj protein,of
R3375 T13008 T13009 compound mr,s
R3376 T13009 T13007 compound s,protein
R3377 T13010 T13009 punct -,s
R3378 T13011 T13000 prep in,confirm
R3379 T13012 T13013 amod mammalian,cells
R3380 T13013 T13011 pobj cells,in
R3381 T13014 T13001 punct ", ",performed
R3382 T13015 T13001 nsubj we,performed
R3383 T13016 T13001 advmod next,performed
R3384 T13017 T13018 compound co-immunoprecipitation,studies
R3385 T13018 T13001 dobj studies,performed
R3386 T13019 T13001 prep in,performed
R3387 T13020 T13021 compound HEK293T,cells
R3388 T13021 T13019 pobj cells,in
R3389 T13022 T13001 prep by,performed
R3390 T13023 T13022 pobj co-transfection,by
R3391 T13024 T13023 prep of,co-transfection
R3392 T13025 T13026 npadvmod HA,tagged
R3393 T13026 T13028 amod tagged,s
R3394 T13027 T13026 punct -,tagged
R3395 T13028 T13024 pobj s,of
R3396 T13029 T13030 amod full,length
R3397 T13030 T13032 dep length,truncated
R3398 T13031 T13030 punct -,length
R3399 T13032 T13028 amod truncated,s
R3400 T13033 T13032 punct /,truncated
R3401 T13034 T13028 compound mr,s
R3402 T13035 T13028 punct -,s
R3403 T13036 T13028 cc and,s
R3404 T13037 T13038 npadvmod Flag,tagged
R3405 T13038 T13040 amod tagged,s
R3406 T13039 T13038 punct -,tagged
R3407 T13040 T13028 conj s,s
R3408 T13041 T13042 amod full,length
R3409 T13042 T13044 dep length,truncated
R3410 T13043 T13042 punct -,length
R3411 T13044 T13040 amod truncated,s
R3412 T13045 T13044 punct /,truncated
R3413 T13046 T13040 compound mr,s
R3414 T13047 T13040 punct -,s
R3415 T13048 T13049 punct (,5A
R3416 T13049 T13001 parataxis 5A,performed
R3417 T13050 T13049 compound Fig.,5A
R3418 T13051 T13049 punct ),5A
R3419 T13052 T13001 punct .,performed
R3420 T13054 T13055 prep As,constructed
R3421 T13056 T13057 det a,control
R3422 T13057 T13054 pobj control,As
R3423 T13058 T13057 amod negative,control
R3424 T13059 T13055 punct ", ",constructed
R3425 T13060 T13055 nsubj we,constructed
R3426 T13061 T13062 npadvmod Flag,tagged
R3427 T13062 T13064 amod tagged,hedgehog
R3428 T13063 T13062 punct -,tagged
R3429 T13064 T13055 dobj hedgehog,constructed
R3430 T13065 T13064 amod Sonic,hedgehog
R3431 T13066 T13064 punct (,hedgehog
R3432 T13067 T13064 appos Shh,hedgehog
R3433 T13068 T13055 punct ),constructed
R3434 T13069 T13070 punct (,2
R3435 T13070 T13055 parataxis 2,constructed
R3436 T13071 T13070 nmod lane,2
R3437 T13072 T13070 cc and,2
R3438 T13073 T13070 conj 7,2
R3439 T13074 T13070 punct ),2
R3440 T13075 T13055 punct .,constructed
R3441 T13077 T13078 prep In,co-immunoprecipitated
R3442 T13079 T13077 pobj accordance,In
R3443 T13080 T13079 prep with,accordance
R3444 T13081 T13082 det the,result
R3445 T13082 T13080 pobj result,with
R3446 T13083 T13082 prep of,result
R3447 T13084 T13085 det the,assay
R3448 T13085 T13083 pobj assay,of
R3449 T13086 T13085 nmod yeast,assay
R3450 T13087 T13088 nummod two,hybrid
R3451 T13088 T13085 compound hybrid,assay
R3452 T13089 T13088 punct -,hybrid
R3453 T13090 T13085 compound GAL4,assay
R3454 T13091 T13078 punct ", ",co-immunoprecipitated
R3455 T13092 T13093 npadvmod HA,tagged
R3456 T13093 T13095 amod tagged,s
R3457 T13094 T13093 punct -,tagged
R3458 T13095 T13078 nsubjpass s,co-immunoprecipitated
R3459 T13096 T13097 amod full,length
R3460 T13097 T13095 compound length,s
R3461 T13098 T13097 punct -,length
R3462 T13099 T13095 compound mr,s
R3463 T13100 T13095 punct -,s
R3464 T13101 T13095 punct (,s
R3465 T13102 T13103 amod full,HA
R3466 T13103 T13095 appos HA,s
R3467 T13104 T13103 punct -,HA
R3468 T13105 T13078 punct ),co-immunoprecipitated
R3469 T13106 T13078 auxpass was,co-immunoprecipitated
R3470 T13107 T13078 prep with,co-immunoprecipitated
R3471 T13108 T13109 npadvmod Flag,tagged
R3472 T13109 T13111 amod tagged,s
R3473 T13110 T13109 punct -,tagged
R3474 T13111 T13107 pobj s,with
R3475 T13112 T13113 amod full,length
R3476 T13113 T13111 compound length,s
R3477 T13114 T13113 punct -,length
R3478 T13115 T13111 compound mr,s
R3479 T13116 T13111 punct -,s
R3480 T13117 T13111 punct (,s
R3481 T13118 T13119 compound Flag,mrs
R3482 T13119 T13111 appos mrs,s
R3483 T13120 T13119 punct -,mrs
R3484 T13121 T13111 punct ),s
R3485 T13122 T13111 cc and,s
R3486 T13123 T13124 det the,portion
R3487 T13124 T13111 conj portion,s
R3488 T13125 T13126 npadvmod Flag,tagged
R3489 T13126 T13124 amod tagged,portion
R3490 T13127 T13126 punct -,tagged
R3491 T13128 T13129 compound C,terminus
R3492 T13129 T13124 compound terminus,portion
R3493 T13130 T13129 punct -,terminus
R3494 T13131 T13124 acl containing,portion
R3495 T13132 T13133 det the,domain
R3496 T13133 T13131 dobj domain,containing
R3497 T13134 T13133 compound SAM,domain
R3498 T13135 T13124 punct (,portion
R3499 T13136 T13137 compound Flag,SAM
R3500 T13137 T13124 appos SAM,portion
R3501 T13138 T13137 punct -,SAM
R3502 T13139 T13078 punct ),co-immunoprecipitated
R3503 T13140 T13078 punct ", ",co-immunoprecipitated
R3504 T13141 T13078 advmod respectively,co-immunoprecipitated
R3505 T13142 T13143 punct (,lane3
R3506 T13143 T13078 parataxis lane3,co-immunoprecipitated
R3507 T13144 T13145 compound Fig,5B
R3508 T13145 T13143 dep 5B,lane3
R3509 T13146 T13143 punct ", ",lane3
R3510 T13147 T13143 cc and,lane3
R3511 T13148 T13143 conj lane5,lane3
R3512 T13149 T13143 punct ),lane3
R3513 T13150 T13078 punct .,co-immunoprecipitated
R3514 T13152 T13153 nsubj We,found
R3515 T13154 T13153 advmod also,found
R3516 T13155 T13156 det a,band
R3517 T13156 T13153 dobj band,found
R3518 T13157 T13156 amod weak,band
R3519 T13158 T13156 compound co-immunoprecipitation,band
R3520 T13159 T13153 prep in,found
R3521 T13160 T13159 pobj co-transfection,in
R3522 T13161 T13160 prep of,co-transfection
R3523 T13162 T13163 amod full,HA
R3524 T13163 T13161 pobj HA,of
R3525 T13164 T13163 punct -,HA
R3526 T13165 T13163 cc and,HA
R3527 T13166 T13167 npadvmod Flag,tagged
R3528 T13167 T13169 amod tagged,portion
R3530 T13168 T13167 punct -,tagged
R3533 T13169 T13163 conj portion,HA
R3536 T13170 T13171 compound N,terminus
R3540 T13171 T13169 compound terminus,portion
R3543 T13172 T13171 punct -,terminus
R3544 T13173 T13169 prep of,portion
R3545 T13174 T13175 compound mr,s
R3546 T13175 T13173 pobj s,of
R3547 T13176 T13175 punct -,s
R3548 T13177 T13169 punct (,portion
R3550 T13178 T13179 compound Flag,ΔSAM
R3552 T13179 T13169 appos ΔSAM,portion
R3555 T13180 T13179 punct -,ΔSAM
R3559 T13181 T13182 punct ", ",lane
R3562 T13182 T13153 parataxis lane,found
R3566 T13183 T13184 compound Fig.,5B
R3570 T13184 T13182 dep 5B,lane
R3573 T13185 T13182 punct ", ",lane
R3577 T13186 T13182 nummod 4,lane
R3581 T13187 T13182 punct ),lane
R3585 T13188 T13153 punct .,found
R3589 T13190 T13191 advmod When,co-transfected
R3590 T13191 T13202 advcl co-transfected,co-immunoprecipitated
R3591 T13277 T13275 pobj s,of
R3592 T13192 T13193 compound ΔSAM,HA
R3593 T13278 T13277 punct -,s
R3594 T13279 T13280 punct (,arrows
R3595 T13280 T13249 parataxis arrows,generated
R3596 T13281 T13282 compound Fig.,1B
R3597 T13282 T13280 dep 1B,arrows
R3598 T13193 T13191 nsubjpass HA,co-transfected
R3599 T13283 T13280 punct ", ",arrows
R3600 T13194 T13193 punct -,HA
R3601 T13195 T13193 cc and,HA
R3602 T13284 T13280 punct ),arrows
R3603 T13196 T13197 npadvmod Flag,tagged
R3604 T13285 T13249 punct .,generated
R3605 T13197 T13199 amod tagged,mutants
R3606 T13287 T13288 det These,mutations
R3607 T13288 T13289 nsubj mutations,alter
R3608 T13198 T13197 punct -,tagged
R3609 T13290 T13289 dobj residues,alter
R3610 T13291 T13292 dep that,conserved
R3611 T13199 T13193 conj mutants,HA
R3612 T13292 T13290 relcl conserved,residues
R3613 T13293 T13292 auxpass are,conserved
R3614 T13294 T13292 prep in,conserved
R3615 T13200 T13199 compound deletion,mutants
R3616 T13295 T13296 det the,domain
R3617 T13296 T13294 pobj domain,in
R3618 T13297 T13296 compound SAM,domain
R3619 T13201 T13191 auxpass were,co-transfected
R3620 T13298 T13296 prep of,domain
R3621 T13299 T13298 pobj ph,of
R3622 T13300 T13289 cc and,alter
R3623 T13203 T13202 punct ", ",co-immunoprecipitated
R3624 T13301 T13302 amod previous,report
R3625 T13302 T13303 nsubj report,indicates
R3626 T13303 T13289 conj indicates,alter
R3627 T13304 T13305 mark that,cause
R3628 T13204 T13205 compound ΔSAM,HA
R3629 T13305 T13303 ccomp cause,indicates
R3630 T13306 T13307 det these,mutations
R3631 T13205 T13202 nsubjpass HA,co-immunoprecipitated
R3632 T13307 T13305 nsubj mutations,cause
R3633 T13308 T13307 prep of,mutations
R3634 T13206 T13205 punct -,HA
R3635 T13309 T13310 compound ph,SAM
R3636 T13310 T13308 pobj SAM,of
R3637 T13311 T13310 punct -,SAM
R3638 T13207 T13202 auxpass was,co-immunoprecipitated
R3639 T13312 T13313 amod significant,reduction
R3640 T13313 T13305 dobj reduction,cause
R3641 T13314 T13313 prep in,reduction
R3642 T13208 T13202 prep with,co-immunoprecipitated
R3643 T13315 T13316 compound binding,activity
R3644 T13316 T13314 pobj activity,in
R3645 T13317 T13316 prep to,activity
R3646 T13209 T13210 compound Flag,mrs
R3647 T13318 T13319 det the,protein
R3648 T13319 T13317 pobj protein,to
R3649 T13320 T13319 amod other,protein
R3650 T13321 T13322 compound SAM,domain
R3651 T13322 T13323 npadvmod domain,containing
R3652 T13210 T13208 pobj mrs,with
R3653 T13323 T13319 amod containing,protein
R3654 T13324 T13323 punct -,containing
R3655 T13325 T13319 punct ", ",protein
R3656 T13326 T13327 compound Sex,comb
R3657 T13211 T13210 punct -,mrs
R3658 T13327 T13319 appos comb,protein
R3659 T13328 T13327 prep on,comb
R3660 T13329 T13328 pobj midleg,on
R3661 T13212 T13210 cc and,mrs
R3662 T13330 T13327 punct (,comb
R3663 T13331 T13327 appos Scm,comb
R3664 T13213 T13214 compound Flag,ΔSAM
R3665 T13332 T13305 punct ),cause
R3666 T13333 T13334 punct (,41
R3667 T13334 T13305 parataxis 41,cause
R3668 T13214 T13210 conj ΔSAM,mrs
R3669 T13335 T13334 punct ),41
R3670 T13336 T13303 punct .,indicates
R3671 T13215 T13214 punct -,ΔSAM
R3672 T13338 T13339 prep Based,introduced
R3673 T13216 T13217 punct (,5B
R3674 T13340 T13338 prep on,Based
R3675 T13341 T13342 det this,result
R3676 T13342 T13340 pobj result,on
R3677 T13217 T13202 parataxis 5B,co-immunoprecipitated
R3678 T13343 T13339 punct ", ",introduced
R3679 T13344 T13339 nsubj we,introduced
R3680 T13345 T13346 nummod two,types
R3681 T13218 T13217 compound Fig.,5B
R3682 T13346 T13339 dobj types,introduced
R3683 T13347 T13346 prep of,types
R3684 T13348 T13349 npadvmod site,directed
R3685 T13219 T13217 punct ", ",5B
R3686 T13349 T13351 amod directed,mutations
R3687 T13350 T13349 punct -,directed
R3688 T13220 T13217 appos lane,5B
R3689 T13351 T13347 pobj mutations,of
R3690 T13352 T13346 punct ", ",types
R3691 T13221 T13220 nummod 8,lane
R3692 T13353 T13354 dep which,correspond
R3693 T13354 T13346 relcl correspond,types
R3694 T13355 T13354 prep to,correspond
R3695 T13222 T13220 cc and,lane
R3696 T13356 T13357 det the,mutations
R3697 T13357 T13355 pobj mutations,to
R3698 T13358 T13357 acl introduced,mutations
R3699 T13223 T13220 conj lane,lane
R3700 T13359 T13358 prep in,introduced
R3701 T13360 T13361 compound ph,protein
R3702 T13361 T13359 pobj protein,in
R3703 T13224 T13223 nummod 9,lane
R3704 T13362 T13358 punct ", ",introduced
R3705 T13225 T13217 punct ),5B
R3706 T13363 T13358 prep into,introduced
R3707 T13364 T13365 npadvmod Flag,tagged
R3708 T13365 T13367 amod tagged,s
R3709 T13226 T13202 punct ", ",co-immunoprecipitated
R3710 T13366 T13365 punct -,tagged
R3711 T13367 T13363 pobj s,into
R3712 T13227 T13228 mark while,co-immunoprecipitated
R3713 T13368 T13369 amod full,length
R3714 T13369 T13367 compound length,s
R3715 T13370 T13369 punct -,length
R3716 T13228 T13202 advcl co-immunoprecipitated,co-immunoprecipitated
R3717 T13371 T13367 compound mr,s
R3718 T13372 T13367 punct -,s
R3719 T13373 T13346 punct (,types
R3720 T13229 T13230 compound ΔSAM,HA
R3721 T13374 T13375 compound Flag,W404A
R3722 T13375 T13346 appos W404A,types
R3723 T13376 T13375 punct -,W404A
R3724 T13230 T13228 nsubjpass HA,co-immunoprecipitated
R3725 T13377 T13375 cc and,W404A
R3726 T13378 T13379 compound Flag,G453A
R3727 T13379 T13375 conj G453A,W404A
R3728 T13231 T13230 punct -,HA
R3729 T13380 T13379 punct -,G453A
R3730 T13381 T13339 punct ),introduced
R3731 T13382 T13339 punct .,introduced
R3732 T13232 T13228 auxpass was,co-immunoprecipitated
R3733 T13384 T13385 nsubj We,found
R3734 T13233 T13228 neg not,co-immunoprecipitated
R3735 T13234 T13228 prep with,co-immunoprecipitated
R3736 T13386 T13387 mark that,reduced
R3737 T13387 T13385 ccomp reduced,found
R3738 T13388 T13389 compound Flag,W404A
R3739 T13235 T13236 compound Flag,SAM
R3740 T13389 T13391 compound W404A,binding
R3741 T13390 T13389 punct -,W404A
R3742 T13391 T13392 compound binding,activity
R3743 T13392 T13387 nsubjpass activity,reduced
R3744 T13393 T13387 auxpass was,reduced
R3745 T13394 T13387 advmod significantly,reduced
R3746 T13395 T13385 cc and,found
R3747 T13396 T13397 compound Flag,G453A
R3748 T13397 T13399 compound G453A,binding
R3749 T13398 T13397 punct -,G453A
R3750 T13399 T13400 compound binding,activity
R3751 T13400 T13401 nsubjpass activity,reduced
R3752 T13401 T13385 conj reduced,found
R3753 T13402 T13401 auxpass was,reduced
R3754 T13403 T13401 advmod also,reduced
R3755 T13404 T13401 advmod slightly,reduced
R3756 T13405 T13401 prep compared,reduced
R3757 T13406 T13405 prep to,compared
R3758 T13407 T13408 compound Flag,mrs
R3759 T13408 T13406 pobj mrs,to
R3760 T13409 T13408 punct -,mrs
R3761 T13410 T13411 punct (,5C
R3762 T13411 T13401 parataxis 5C,reduced
R3763 T13412 T13411 compound Fig.,5C
R3764 T13413 T13411 punct ),5C
R3765 T13414 T13401 punct .,reduced
R3766 T13416 T13417 det These,results
R3767 T13417 T13418 nsubj results,indicate
R3768 T13419 T13418 punct ", ",indicate
R3769 T13420 T13418 advmod together,indicate
R3770 T13421 T13420 prep with,together
R3771 T13422 T13423 nmod yeast,assay
R3772 T13423 T13421 pobj assay,with
R3773 T13424 T13425 nummod two,hybrid
R3774 T13425 T13423 compound hybrid,assay
R3775 T13426 T13425 punct -,hybrid
R3776 T13427 T13423 compound GAL4,assay
R3777 T13428 T13418 punct ", ",indicate
R3778 T13429 T13430 mark that,associates
R3779 T13430 T13418 ccomp associates,indicate
R3780 T13431 T13432 det the,protein
R3781 T13432 T13430 nsubj protein,associates
R3782 T13433 T13434 compound mr,s
R3783 T13434 T13432 compound s,protein
R3784 T13435 T13434 punct -,s
R3785 T13436 T13430 dep self,associates
R3786 T13437 T13430 punct -,associates
R3787 T13438 T13430 advmod strongly,associates
R3788 T13439 T13430 prep through,associates
R3789 T13440 T13441 poss its,domain
R3790 T13441 T13439 pobj domain,through
R3791 T13442 T13441 compound SAM,domain
R3792 T13443 T13430 cc and,associates
R3793 T13444 T13430 conj weakly,associates
R3794 T13445 T13444 prep through,weakly
R3795 T13446 T13447 det the,portion
R3796 T13447 T13445 pobj portion,through
R3797 T13448 T13449 compound N,terminus
R3798 T13449 T13447 compound terminus,portion
R3799 T13450 T13449 punct -,terminus
R3800 T13451 T13447 acl lacking,portion
R3801 T13452 T13453 compound SAM,domain
R3802 T13453 T13451 dobj domain,lacking
R3803 T13454 T13418 punct .,indicate
R8797 T30893 T30892 prep of,Summary
R8798 T30894 T30895 nmod yeast,screening
R8799 T30895 T30893 pobj screening,of
R8800 T30896 T30897 nummod two,hybrid
R8801 T30897 T30895 compound hybrid,screening
R8802 T30898 T30897 punct -,hybrid
R8803 T30899 T30895 cc and,screening
R8804 T30900 T30901 compound GAL4,assay
R8805 T30901 T30895 conj assay,screening
R8806 T30902 T30892 punct .,Summary
R8807 T30904 T30905 punct (,A
R8808 T30905 T30906 meta A,indicated
R8809 T30907 T30905 punct ),A
R8810 T30908 T30909 amod Full,length
R8811 T30909 T30911 compound length,s
R8812 T30910 T30909 punct -,length
R8813 T30911 T30906 nsubjpass s,indicated
R8814 T30912 T30911 compound mr,s
R8815 T30913 T30911 punct -,s
R8816 T30914 T30911 prep as,s
R8817 T30915 T30916 det a,bait
R8818 T30916 T30914 pobj bait,as
R8819 T30917 T30916 acl used,bait
R8820 T30918 T30917 prep in,used
R8821 T30919 T30920 det the,screening
R8822 T30920 T30918 pobj screening,in
R8823 T30921 T30920 nmod yeast,screening
R8824 T30922 T30923 nummod two,hybrid
R8825 T30923 T30920 compound hybrid,screening
R8826 T30924 T30923 punct -,hybrid
R8827 T30925 T30911 cc and,s
R8828 T30926 T30927 amod positive,clones
R8829 T30927 T30911 conj clones,s
R8830 T30928 T30906 auxpass are,indicated
R8831 T30929 T30906 punct .,indicated
R8832 T30932 T30933 mark that,contain
R8833 T30933 T30931 ccomp contain,Note
R8834 T30934 T30933 nsubj all,contain
R8835 T30935 T30934 prep of,all
R8836 T30936 T30937 nummod five,clones
R8837 T30937 T30935 pobj clones,of
R8838 T30938 T30939 compound mr,s
R8839 T30939 T30937 compound s,clones
R8840 T30940 T30939 punct -,s
R8841 T30941 T30937 acl identified,clones
R8842 T30942 T30941 prep in,identified
R8843 T30943 T30944 det the,screening
R8844 T30944 T30942 pobj screening,in
R8845 T30945 T30946 compound SAM,domains
R8846 T30946 T30933 dobj domains,contain
R8847 T30947 T30931 punct .,Note
R8848 T30949 T30950 punct (,B
R8849 T30950 T30951 meta B,diagram
R8850 T30952 T30950 punct ),B
R8851 T30953 T30951 amod Schematic,diagram
R8852 T30954 T30951 prep of,diagram
R8853 T30955 T30956 det the,proteins
R8854 T30956 T30954 pobj proteins,of
R8855 T30957 T30958 compound mr,s
R8856 T30958 T30956 compound s,proteins
R8857 T30959 T30958 punct -,s
R8858 T30960 T30956 compound fusion,proteins
R8859 T30961 T30956 acl used,proteins
R8860 T30962 T30961 prep in,used
R8861 T30963 T30964 det the,assay
R8862 T30964 T30962 pobj assay,in
R8863 T30965 T30964 compound yeast,assay
R8864 T30966 T30964 compound GAL4,assay
R8865 T30967 T30951 punct .,diagram
R8866 T30969 T30970 amod Black,boxes
R8867 T30970 T30971 nsubj boxes,represent
R8868 T30972 T30973 det the,position
R8869 T30973 T30971 dobj position,represent
R8870 T30974 T30973 prep of,position
R8871 T30975 T30976 compound SAM,domains
R8872 T30976 T30974 pobj domains,of
R8873 T30977 T30971 punct .,represent
R8874 T30979 T30980 amod Relative,levels
R8875 T30980 T30981 nsubjpass levels,shown
R8876 T30982 T30980 prep of,levels
R8877 T30983 T30984 compound LacZ,expression
R8878 T30984 T30982 pobj expression,of
R8879 T30985 T30981 auxpass are,shown
R8880 T30986 T30981 prep on,shown
R8881 T30987 T30988 det the,right
R8882 T30988 T30986 pobj right,on
R8883 T30989 T30981 punct ", ",shown
R8884 T30990 T30981 advmod respectively,shown
R8885 T30991 T30981 punct .,shown
R8886 T30993 T30994 dep Note,indicates
R8887 T30995 T30994 punct ;,indicates
R8888 T30996 T30997 nsubj ++,indicates
R8889 T30997 T30994 ccomp indicates,indicates
R8890 T30998 T30999 det an,signal
R8891 T30999 T30997 dobj signal,indicates
R8892 T31000 T30999 amod intense,signal
R8893 T31001 T30999 amod blue,signal
R8894 T31002 T30999 relcl visible,signal
R8895 T31003 T31002 prep after,visible
R8896 T31004 T31003 pobj 12hr,after
R8897 T31005 T31004 prep of,12hr
R8898 T31006 T31005 pobj incubation,of
R8899 T31007 T31006 prep at,incubation
R8900 T31008 T31009 nummod 37,°C
R8901 T31009 T31007 pobj °C,at
R8902 T31010 T30994 punct ", ",indicates
R8903 T31011 T30994 nsubj +,indicates
R8904 T31012 T31013 det a,signal
R8905 T31013 T30994 dobj signal,indicates
R8906 T31014 T31013 amod blue,signal
R8907 T31015 T31013 relcl visible,signal
R8908 T31016 T31015 prep after,visible
R8909 T31017 T31016 pobj 24hr,after
R8910 T31018 T31017 prep of,24hr
R8911 T31019 T31018 pobj incubation,of
R8912 T31020 T30994 punct .,indicates
R8913 T31023 T31022 punct ", ",BD
R8914 T31024 T31025 compound binding,domain
R8915 T31025 T31022 appos domain,BD
R8916 T31026 T31022 punct ;,BD
R8917 T31027 T31022 appos AD,BD
R8918 T31028 T31027 punct ", ",AD
R8919 T31029 T31030 compound activation,domain
R8920 T31030 T31027 appos domain,AD
R8921 T31031 T31022 punct ;,BD
R8922 T31032 T31033 amod full,s
R8923 T31033 T31022 appos s,BD
R8924 T31034 T31033 punct ", ",s
R8925 T31035 T31036 amod full,length
R8926 T31036 T31033 compound length,s
R8927 T31037 T31036 punct -,length
R8928 T31038 T31033 compound mr,s
R8929 T31039 T31033 punct -,s
R8930 T31040 T31022 punct ;,BD
R8931 T31041 T31022 appos N,BD
R8932 T31042 T31041 punct ", ",N
R8933 T31043 T31044 npadvmod N,terminal
R8934 T31044 T31046 amod terminal,portion
R8935 T31045 T31044 punct -,terminal
R8936 T31046 T31041 appos portion,N
R8937 T31047 T31046 prep of,portion
R8938 T31048 T31049 compound mr,s
R8939 T31049 T31047 pobj s,of
R8940 T31050 T31049 punct -,s
R8941 T31051 T31052 punct (,1
R8942 T31052 T31046 parataxis 1,portion
R8943 T31053 T31052 nmod amino,1
R8944 T31054 T31052 nmod acids,1
R8945 T31055 T31052 prep to,1
R8946 T31056 T31055 pobj 400,to
R8947 T31057 T31052 punct ),1
R8948 T31058 T31022 punct ;,BD
R8949 T31059 T31022 appos C,BD
R8950 T31060 T31059 punct ", ",C
R8951 T31061 T31062 npadvmod C,terminal
R8952 T31062 T31064 amod terminal,portion
R8953 T31063 T31062 punct -,terminal
R8954 T31064 T31059 appos portion,C
R8955 T31065 T31064 prep of,portion
R8956 T31066 T31067 compound mr,s
R8957 T31067 T31065 pobj s,of
R8958 T31068 T31067 punct -,s
R8959 T31069 T31070 punct (,391
R8960 T31070 T31064 parataxis 391,portion
R8961 T31071 T31070 nmod amino,391
R8962 T31072 T31070 nmod acids,391
R8963 T31073 T31070 prep to,391
R8964 T31074 T31073 pobj 542,to
R8965 T31075 T31070 punct ),391
R8966 T31076 T31022 punct .,BD
R8967 T31407 T31408 det The,protein
R8968 T31408 T31412 nsubj protein,associate
R8969 T31409 T31410 compound mr,s
R8970 T31410 T31408 compound s,protein
R8971 T31411 T31410 punct -,s
R8972 T31413 T31412 aux can,associate
R8973 T31414 T31412 dep self,associate
R8974 T31415 T31412 punct -,associate
R8975 T31416 T31412 prep in,associate
R8976 T31417 T31418 amod mammalian,cells
R8977 T31418 T31416 pobj cells,in
R8978 T31419 T31412 punct .,associate
R8979 T31421 T31422 punct (,A
R8980 T31422 T31423 meta A,drawing
R8981 T31424 T31422 punct ),A
R8982 T31425 T31423 amod Schematic,drawing
R8983 T31426 T31423 prep of,drawing
R8984 T31427 T31428 det the,constructs
R8985 T31428 T31426 pobj constructs,of
R8986 T31429 T31428 acl used,constructs
R8987 T31430 T31429 prep for,used
R8988 T31431 T31432 compound immunoprecipitation,assay
R8989 T31432 T31430 pobj assay,for
R8990 T31433 T31423 punct .,drawing
R8991 T31435 T31436 npadvmod HA,tagged
R8992 T31436 T31438 amod tagged,regions
R8993 T31437 T31436 punct -,tagged
R8994 T31438 T31471 nsubjpass regions,inserted
R8995 T31439 T31436 cc or,tagged
R8996 T31440 T31441 npadvmod Flag,tagged
R8997 T31441 T31436 conj tagged,tagged
R8998 T31442 T31441 punct -,tagged
R8999 T31443 T31444 amod full,length
R9000 T31444 T31438 nmod length,regions
R9001 T31445 T31444 punct -,length
R9002 T31446 T31447 punct (,1
R9003 T31447 T31444 parataxis 1,length
R9004 T31448 T31447 nmod amino,1
R9005 T31449 T31447 nmod acids,1
R9006 T31450 T31447 prep to,1
R9007 T31451 T31450 pobj 542,to
R9008 T31452 T31447 punct ),1
R9009 T31453 T31438 punct ", ",regions
R9010 T31454 T31438 nmod ΔSAM,regions
R9011 T31455 T31456 punct (,1
R9012 T31456 T31454 parataxis 1,ΔSAM
R9013 T31457 T31456 nmod amino,1
R9014 T31458 T31456 nmod acids,1
R9015 T31459 T31456 prep to,1
R9016 T31460 T31459 pobj 400,to
R9017 T31461 T31456 punct ),1
R9018 T31462 T31454 cc and,ΔSAM
R9019 T31463 T31454 conj SAM,ΔSAM
R9020 T31464 T31465 punct (,401
R9021 T31465 T31463 parataxis 401,SAM
R9022 T31466 T31465 nmod amino,401
R9023 T31467 T31465 nmod acids,401
R9024 T31468 T31465 prep to,401
R9025 T31469 T31468 pobj 542,to
R9026 T31470 T31465 punct ),401
R9027 T31472 T31471 auxpass were,inserted
R9028 T31473 T31471 prep into,inserted
R9029 T31474 T31475 compound pcDNA3,vector
R9030 T31475 T31473 pobj vector,into
R9031 T31476 T31471 punct ", ",inserted
R9032 T31477 T31471 advmod respectively,inserted
R9033 T31478 T31471 punct .,inserted
R9034 T31480 T31481 punct (,B
R9035 T31481 T31482 meta B,transfected
R9036 T31483 T31481 punct ),B
R9037 T31484 T31485 det The,constructs
R9038 T31485 T31482 nsubjpass constructs,transfected
R9039 T31486 T31485 acl indicated,constructs
R9040 T31487 T31486 advmod above,indicated
R9041 T31488 T31482 auxpass were,transfected
R9042 T31489 T31482 prep into,transfected
R9043 T31490 T31491 compound HEK293T,cells
R9044 T31491 T31489 pobj cells,into
R9045 T31492 T31482 punct .,transfected
R9046 T31494 T31495 det Each,lane
R9047 T31495 T31496 nsubjpass lane,co-immunoprecipitated
R9048 T31497 T31496 auxpass was,co-immunoprecipitated
R9049 T31498 T31496 agent by,co-immunoprecipitated
R9050 T31499 T31500 amod anti-Flag,antibody
R9051 T31500 T31498 pobj antibody,by
R9052 T31501 T31496 cc and,co-immunoprecipitated
R9053 T31502 T31496 conj detected,co-immunoprecipitated
R9054 T31503 T31502 agent by,detected
R9055 T31504 T31505 amod anti-HA,antibody
R9056 T31505 T31503 pobj antibody,by
R9057 T31506 T31496 punct .,co-immunoprecipitated
R9058 T31508 T31509 compound Input,lysates
R9059 T31509 T31511 nsubjpass lysates,shown
R9060 T31510 T31509 compound protein,lysates
R9061 T31512 T31511 auxpass are,shown
R9062 T31513 T31511 prep in,shown
R9063 T31514 T31515 det the,panels
R9064 T31515 T31513 pobj panels,in
R9065 T31516 T31515 amod lower,panels
R9066 T31517 T31511 punct .,shown
R9067 T31519 T31520 punct (,C
R9068 T31520 T31521 meta C,generated
R9069 T31522 T31520 punct ),C
R9070 T31523 T31524 npadvmod Flag,tagged
R9071 T31524 T31526 amod tagged,mutants
R9072 T31525 T31524 punct -,tagged
R9073 T31526 T31521 nsubjpass mutants,generated
R9074 T31527 T31528 nummod two,site
R9075 T31528 T31529 npadvmod site,directed
R9076 T31529 T31526 amod directed,mutants
R9077 T31530 T31529 punct -,directed
R9078 T31531 T31532 compound mr,s
R9079 T31532 T31526 compound s,mutants
R9080 T31533 T31532 punct -,s
R9081 T31534 T31526 punct ", ",mutants
R9082 T31535 T31536 compound Flag,W404A
R9083 T31536 T31526 appos W404A,mutants
R9084 T31537 T31536 punct -,W404A
R9085 T31538 T31536 cc and,W404A
R9086 T31539 T31540 compound Flag,G453A
R9087 T31540 T31536 conj G453A,W404A
R9088 T31541 T31540 punct -,G453A
R9089 T31542 T31521 auxpass were,generated
R9090 T31543 T31521 cc and,generated
R9091 T31544 T31521 conj co-transfected,generated
R9092 T31545 T31544 prep with,co-transfected
R9093 T31546 T31547 amod full,HA
R9094 T31547 T31545 pobj HA,with
R9095 T31548 T31547 punct -,HA
R9096 T31549 T31521 punct .,generated
R9097 T31551 T31552 det Each,lane
R9098 T31552 T31553 nsubjpass lane,co-immunoprecipitated
R9099 T31554 T31553 auxpass was,co-immunoprecipitated
R9100 T31555 T31553 agent by,co-immunoprecipitated
R9101 T31556 T31557 amod anti-HA,antibody
R9102 T31557 T31555 pobj antibody,by
R9103 T31558 T31553 cc and,co-immunoprecipitated
R9104 T31559 T31553 conj detected,co-immunoprecipitated
R9105 T31560 T31559 agent by,detected
R9106 T31561 T31562 amod anti-Flag,antibody
R9107 T31562 T31560 pobj antibody,by
R9108 T31563 T31553 punct .,co-immunoprecipitated
R3293 T12919 T12917 ccomp was,indicated
R3297 T12923 T12921 relcl was,signal
R3305 T12931 T12923 acomp present,was
R3306 T12932 T12923 prep in,was
R3307 T12933 T12934 preconj both,contexts
R3308 T12934 T12932 pobj contexts,in
R3309 T12935 T12934 det the,contexts
R3310 T12936 T12934 nmod bait,contexts
R3311 T12937 T12936 cc and,bait
R3312 T12938 T12936 conj prey,bait
R3313 T12939 T12919 acomp weaker,was
R3314 T12940 T12939 prep than,weaker
R3315 T12941 T12942 advmod when,used
R3316 T12942 T12940 pcomp used,than
R3317 T12943 T12944 amod isolated,domains
R3318 T12944 T12942 nsubjpass domains,used
R3367 T12999 T13000 aux To,confirm
R3368 T13000 T13001 advcl confirm,performed
R3369 T13002 T13003 compound self,association
R3370 T13003 T13000 dobj association,confirm
R3371 T13004 T13003 punct -,association
R3529 T13236 T13234 pobj SAM,with
R3531 T13237 T13236 punct -,SAM
R3532 T13238 T13239 punct (,5B
R3534 T13239 T13228 parataxis 5B,co-immunoprecipitated
R3535 T13240 T13239 compound Fig.,5B
R3537 T13241 T13239 punct ", ",5B
R3538 T13242 T13239 appos lane,5B
R3539 T13243 T13242 nummod 10,lane
R3541 T13244 T13239 punct ),5B
R3542 T13245 T13202 punct .,co-immunoprecipitated
R3549 T13247 T13248 aux To,investigate
R3551 T13248 T13249 advcl investigate,generated
R3553 T13250 T13251 mark whether,associates
R3554 T13251 T13248 ccomp associates,investigate
R3556 T13252 T13253 det the,protein
R3557 T13253 T13251 nsubj protein,associates
R3558 T13254 T13255 compound mr,s
R3560 T13255 T13253 compound s,protein
R3561 T13256 T13255 punct -,s
R3563 T13257 T13251 dep self,associates
R3564 T13258 T13251 punct -,associates
R3565 T13259 T13251 advmod mainly,associates
R3567 T13260 T13251 prep through,associates
R3568 T13261 T13262 det the,domain
R3569 T13262 T13260 pobj domain,through
R3571 T13263 T13262 compound SAM,domain
R3572 T13264 T13249 punct ", ",generated
R3574 T13265 T13266 nummod two,mutations
R3575 T13266 T13249 nsubjpass mutations,generated
R3576 T13267 T13268 npadvmod site,directed
R3578 T13268 T13266 amod directed,mutations
R3579 T13269 T13268 punct -,directed
R3580 T13270 T13249 auxpass were,generated
R3582 T13271 T13249 prep in,generated
R3583 T13272 T13273 det the,domain
R3584 T13273 T13271 pobj domain,in
R3586 T13274 T13273 compound SAM,domain
R3587 T13275 T13273 prep of,domain
R3588 T13276 T13277 compound mr,s