PMC:138691 / 7499-8663 JSONTXT

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T3190","span":{"begin":113,"end":135},"obj":"GO_EXT:0000150"},{"id":"T3191","span":{"begin":136,"end":141},"obj":"PR_EXT:000013672"},{"id":"T3192","span":{"begin":148,"end":155},"obj":"CHEBI_SO_EXT:peptide_or_peptide_region"},{"id":"T3193","span":{"begin":191,"end":198},"obj":"SO_EXT:sequence_repeat_unit_or_region"},{"id":"T3194","span":{"begin":204,"end":209},"obj":"NCBITaxon:9606"},{"id":"T3195","span":{"begin":210,"end":215},"obj":"PR_EXT:000004804"},{"id":"T3196","span":{"begin":217,"end":227},"obj":"GO:0010467"},{"id":"T3197","span":{"begin":244,"end":250},"obj":"SO_EXT:sequence_repeat_unit_or_region"},{"id":"T3198","span":{"begin":300,"end":309},"obj":"CHEBI_EXT:35222"},{"id":"T3199","span":{"begin":313,"end":316},"obj":"CHEBI_SO_EXT:DNA"},{"id":"T3200","span":{"begin":313,"end":323},"obj":"GO:0006281"},{"id":"T3201","span":{"begin":376,"end":386},"obj":"GO:0010467"},{"id":"T3202","span":{"begin":390,"end":400},"obj":"SO_EXT:engineered_biological_sequence"},{"id":"T3203","span":{"begin":421,"end":427},"obj":"SO_EXT:sequence_repeat_unit_or_region"},{"id":"T3204","span":{"begin":437,"end":442},"obj":"CL_GO_EXT:cell"},{"id":"T3205","span":{"begin":476,"end":481},"obj":"CL_GO_EXT:cell"},{"id":"T3206","span":{"begin":502,"end":506},"obj":"GO:0000086"},{"id":"T3207","span":{"begin":559,"end":570},"obj":"GO:0009294"},{"id":"T3208","span":{"begin":571,"end":576},"obj":"CL_GO_EXT:cell"},{"id":"T3209","span":{"begin":592,"end":599},"obj":"GO:0005634"},{"id":"T3210","span":{"begin":600,"end":605},"obj":"PR_EXT:000013672"},{"id":"T3211","span":{"begin":738,"end":748},"obj":"GO:0010467"},{"id":"T3212","span":{"begin":765,"end":771},"obj":"SO_EXT:sequence_repeat_unit_or_region"},{"id":"T3213","span":{"begin":773,"end":776},"obj":"CHEBI_SO_EXT:DNA"},{"id":"T3214","span":{"begin":777,"end":784},"obj":"CHEMINF_GO_EXT:chemical_binding_or_bond_formation"},{"id":"T3215","span":{"begin":796,"end":804},"obj":"CHEBI:10545"},{"id":"T3216","span":{"begin":854,"end":861},"obj":"CHEBI_SO_EXT:peptide_or_peptide_region"},{"id":"T3217","span":{"begin":893,"end":898},"obj":"PR_EXT:000013672"},{"id":"T3218","span":{"begin":916,"end":926},"obj":"GO_EXT:oligomeric_macromolecular_complex"},{"id":"T3219","span":{"begin":940,"end":943},"obj":"CHEBI_SO_EXT:DNA"},{"id":"T3220","span":{"begin":952,"end":959},"obj":"CHEBI_SO_EXT:peptide_or_peptide_region"},{"id":"T3221","span":{"begin":993,"end":998},"obj":"PR_EXT:000013672"},{"id":"T3222","span":{"begin":999,"end":1002},"obj":"CHEBI_SO_EXT:DNA"},{"id":"T3223","span":{"begin":1077,"end":1082},"obj":"PR_EXT:000004804"},{"id":"T3224","span":{"begin":1083,"end":1090},"obj":"CHEBI_PR_EXT:protein"},{"id":"T3225","span":{"begin":1106,"end":1124},"obj":"GO_EXT:negative_regulator"},{"id":"T3226","span":{"begin":1142,"end":1147},"obj":"PR_EXT:000013672"},{"id":"T3227","span":{"begin":1148,"end":1151},"obj":"CHEBI_SO_EXT:DNA"}],"text":"The second paper providing evidence, by Davies et al [26], is a biochemical study of the interaction between the homologous recombinase RAD51 and a peptide consisting of one of the eight BRC repeats from human BRCA2. Expression of a single BRC repeat (BRC4) had previously been reported to act as an inhibitor of DNA repair by Chen et al [27]. These investigators showed that expression of constructs containing the BRC4 repeat in MCF-7 cells enhances the radiosensitivity of cells and blocks both the G2/M delay associated with damage and the ability of the transfected cells to assembly subnuclear RAD51 foci. In the paper by Davies et al, data provide evidence for a plausible mechanism of the dominant negative effect associated with expression of a single BRC repeat. DNA binding assays and electron microscopy methods were used to show that a BRC3 peptide interferes with the ability of RAD51 to assemble into oligomeric filaments on DNA. A BRC4 peptide was also reported to inhibit the RAD51–DNA interaction. These experiments raise the possibility that the full-length BRCA2 protein could act as a negative regulator of inappropriate RAD51–DNA interaction."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T2031","span":{"begin":136,"end":141},"obj":"PR:000013672"},{"id":"T2032","span":{"begin":204,"end":209},"obj":"NCBITaxon:9606"},{"id":"T2033","span":{"begin":210,"end":215},"obj":"PR:000004804"},{"id":"T2034","span":{"begin":217,"end":227},"obj":"GO:0010467"},{"id":"T2035","span":{"begin":300,"end":309},"obj":"CHEBI:35222"},{"id":"T2036","span":{"begin":313,"end":323},"obj":"GO:0006281"},{"id":"T2037","span":{"begin":376,"end":386},"obj":"GO:0010467"},{"id":"T2038","span":{"begin":502,"end":506},"obj":"GO:0000086"},{"id":"T2039","span":{"begin":559,"end":570},"obj":"GO:0009294"},{"id":"T2040","span":{"begin":592,"end":599},"obj":"GO:0005634"},{"id":"T2041","span":{"begin":600,"end":605},"obj":"PR:000013672"},{"id":"T2042","span":{"begin":738,"end":748},"obj":"GO:0010467"},{"id":"T2043","span":{"begin":796,"end":804},"obj":"CHEBI:10545"},{"id":"T2044","span":{"begin":893,"end":898},"obj":"PR:000013672"},{"id":"T2045","span":{"begin":993,"end":998},"obj":"PR:000013672"},{"id":"T2046","span":{"begin":1077,"end":1082},"obj":"PR:000004804"},{"id":"T2047","span":{"begin":1142,"end":1147},"obj":"PR:000013672"}],"text":"The second paper providing evidence, by Davies et al [26], is a biochemical study of the interaction between the homologous recombinase RAD51 and a peptide consisting of one of the eight BRC repeats from human BRCA2. Expression of a single BRC repeat (BRC4) had previously been reported to act as an inhibitor of DNA repair by Chen et al [27]. These investigators showed that expression of constructs containing the BRC4 repeat in MCF-7 cells enhances the radiosensitivity of cells and blocks both the G2/M delay associated with damage and the ability of the transfected cells to assembly subnuclear RAD51 foci. In the paper by Davies et al, data provide evidence for a plausible mechanism of the dominant negative effect associated with expression of a single BRC repeat. DNA binding assays and electron microscopy methods were used to show that a BRC3 peptide interferes with the ability of RAD51 to assemble into oligomeric filaments on DNA. A BRC4 peptide was also reported to inhibit the RAD51–DNA interaction. These experiments raise the possibility that the full-length BRCA2 protein could act as a negative regulator of inappropriate RAD51–DNA interaction."}

{"project":"craft-sa-dev","denotations":[{"id":"T2154","span":{"begin":0,"end":3},"obj":"DT"},{"id":"T2155","span":{"begin":11,"end":16},"obj":"NN"},{"id":"T2156","span":{"begin":4,"end":10},"obj":"JJ"},{"id":"T2157","span":{"begin":59,"end":61},"obj":"VBZ"},{"id":"T2158","span":{"begin":17,"end":26},"obj":"VBG"},{"id":"T2159","span":{"begin":27,"end":35},"obj":"NN"},{"id":"T2160","span":{"begin":35,"end":37},"obj":","},{"id":"T2161","span":{"begin":37,"end":39},"obj":"IN"},{"id":"T2162","span":{"begin":40,"end":46},"obj":"NNP"},{"id":"T2163","span":{"begin":47,"end":49},"obj":"FW"},{"id":"T2164","span":{"begin":50,"end":52},"obj":"FW"},{"id":"T2165","span":{"begin":53,"end":54},"obj":"-LRB-"},{"id":"T2166","span":{"begin":54,"end":56},"obj":"CD"},{"id":"T2167","span":{"begin":56,"end":57},"obj":"-RRB-"},{"id":"T2168","span":{"begin":57,"end":59},"obj":","},{"id":"T2169","span":{"begin":62,"end":63},"obj":"DT"},{"id":"T2170","span":{"begin":76,"end":81},"obj":"NN"},{"id":"T2171","span":{"begin":64,"end":75},"obj":"JJ"},{"id":"T2172","span":{"begin":82,"end":84},"obj":"IN"},{"id":"T2173","span":{"begin":85,"end":88},"obj":"DT"},{"id":"T2174","span":{"begin":89,"end":100},"obj":"NN"},{"id":"T2175","span":{"begin":101,"end":108},"obj":"IN"},{"id":"T2176","span":{"begin":109,"end":112},"obj":"DT"},{"id":"T2177","span":{"begin":136,"end":141},"obj":"NN"},{"id":"T2178","span":{"begin":113,"end":123},"obj":"JJ"},{"id":"T2179","span":{"begin":124,"end":135},"obj":"NN"},{"id":"T2180","span":{"begin":142,"end":145},"obj":"CC"},{"id":"T2181","span":{"begin":146,"end":147},"obj":"DT"},{"id":"T2182","span":{"begin":148,"end":155},"obj":"NN"},{"id":"T2183","span":{"begin":156,"end":166},"obj":"VBG"},{"id":"T2184","span":{"begin":167,"end":169},"obj":"IN"},{"id":"T2185","span":{"begin":170,"end":173},"obj":"CD"},{"id":"T2186","span":{"begin":174,"end":176},"obj":"IN"},{"id":"T2187","span":{"begin":177,"end":180},"obj":"DT"},{"id":"T2188","span":{"begin":191,"end":198},"obj":"NNS"},{"id":"T2189","span":{"begin":181,"end":186},"obj":"CD"},{"id":"T2190","span":{"begin":187,"end":190},"obj":"NN"},{"id":"T2191","span":{"begin":199,"end":203},"obj":"IN"},{"id":"T2192","span":{"begin":204,"end":209},"obj":"JJ"},{"id":"T2193","span":{"begin":210,"end":215},"obj":"NN"},{"id":"T2194","span":{"begin":215,"end":216},"obj":"."},{"id":"T2195","span":{"begin":216,"end":343},"obj":"sentence"},{"id":"T2196","span":{"begin":217,"end":227},"obj":"NN"},{"id":"T2197","span":{"begin":278,"end":286},"obj":"VBN"},{"id":"T2198","span":{"begin":228,"end":230},"obj":"IN"},{"id":"T2199","span":{"begin":231,"end":232},"obj":"DT"},{"id":"T2200","span":{"begin":244,"end":250},"obj":"NN"},{"id":"T2201","span":{"begin":233,"end":239},"obj":"JJ"},{"id":"T2202","span":{"begin":240,"end":243},"obj":"NN"},{"id":"T2203","span":{"begin":251,"end":252},"obj":"-LRB-"},{"id":"T2204","span":{"begin":252,"end":256},"obj":"NN"},{"id":"T2205","span":{"begin":256,"end":257},"obj":"-RRB-"},{"id":"T2206","span":{"begin":258,"end":261},"obj":"VBD"},{"id":"T2207","span":{"begin":262,"end":272},"obj":"RB"},{"id":"T2208","span":{"begin":273,"end":277},"obj":"VBN"},{"id":"T2209","span":{"begin":287,"end":289},"obj":"TO"},{"id":"T2210","span":{"begin":290,"end":293},"obj":"VB"},{"id":"T2211","span":{"begin":294,"end":296},"obj":"IN"},{"id":"T2212","span":{"begin":297,"end":299},"obj":"DT"},{"id":"T2213","span":{"begin":300,"end":309},"obj":"NN"},{"id":"T2214","span":{"begin":310,"end":312},"obj":"IN"},{"id":"T2215","span":{"begin":313,"end":316},"obj":"NN"},{"id":"T2216","span":{"begin":317,"end":323},"obj":"NN"},{"id":"T2217","span":{"begin":324,"end":326},"obj":"IN"},{"id":"T2218","span":{"begin":327,"end":331},"obj":"NNP"},{"id":"T2219","span":{"begin":332,"end":334},"obj":"FW"},{"id":"T2220","span":{"begin":335,"end":337},"obj":"FW"},{"id":"T2221","span":{"begin":338,"end":339},"obj":"-LRB-"},{"id":"T2222","span":{"begin":339,"end":341},"obj":"CD"},{"id":"T2223","span":{"begin":341,"end":342},"obj":"-RRB-"},{"id":"T2224","span":{"begin":342,"end":343},"obj":"."},{"id":"T2225","span":{"begin":343,"end":611},"obj":"sentence"},{"id":"T2226","span":{"begin":344,"end":349},"obj":"DT"},{"id":"T2227","span":{"begin":350,"end":363},"obj":"NNS"},{"id":"T2228","span":{"begin":364,"end":370},"obj":"VBD"},{"id":"T2229","span":{"begin":371,"end":375},"obj":"IN"},{"id":"T2230","span":{"begin":443,"end":451},"obj":"VBZ"},{"id":"T2231","span":{"begin":376,"end":386},"obj":"NN"},{"id":"T2232","span":{"begin":387,"end":389},"obj":"IN"},{"id":"T2233","span":{"begin":390,"end":400},"obj":"NNS"},{"id":"T2234","span":{"begin":401,"end":411},"obj":"VBG"},{"id":"T2235","span":{"begin":412,"end":415},"obj":"DT"},{"id":"T2236","span":{"begin":421,"end":427},"obj":"NN"},{"id":"T2237","span":{"begin":416,"end":420},"obj":"NN"},{"id":"T2238","span":{"begin":428,"end":430},"obj":"IN"},{"id":"T2239","span":{"begin":431,"end":434},"obj":"NN"},{"id":"T2240","span":{"begin":437,"end":442},"obj":"NNS"},{"id":"T2241","span":{"begin":434,"end":435},"obj":"HYPH"},{"id":"T2242","span":{"begin":435,"end":436},"obj":"CD"},{"id":"T2243","span":{"begin":452,"end":455},"obj":"DT"},{"id":"T2244","span":{"begin":456,"end":472},"obj":"NN"},{"id":"T2245","span":{"begin":473,"end":475},"obj":"IN"},{"id":"T2246","span":{"begin":476,"end":481},"obj":"NNS"},{"id":"T2247","span":{"begin":482,"end":485},"obj":"CC"},{"id":"T2248","span":{"begin":486,"end":492},"obj":"VBZ"},{"id":"T2249","span":{"begin":493,"end":497},"obj":"CC"},{"id":"T2250","span":{"begin":507,"end":512},"obj":"NN"},{"id":"T2251","span":{"begin":498,"end":501},"obj":"DT"},{"id":"T2252","span":{"begin":502,"end":504},"obj":"NN"},{"id":"T2253","span":{"begin":505,"end":506},"obj":"NN"},{"id":"T2254","span":{"begin":504,"end":505},"obj":"HYPH"},{"id":"T2255","span":{"begin":513,"end":523},"obj":"VBN"},{"id":"T2256","span":{"begin":524,"end":528},"obj":"IN"},{"id":"T2257","span":{"begin":529,"end":535},"obj":"NN"},{"id":"T2258","span":{"begin":536,"end":539},"obj":"CC"},{"id":"T2259","span":{"begin":540,"end":543},"obj":"DT"},{"id":"T2260","span":{"begin":544,"end":551},"obj":"NN"},{"id":"T2261","span":{"begin":552,"end":554},"obj":"IN"},{"id":"T2262","span":{"begin":555,"end":558},"obj":"DT"},{"id":"T2263","span":{"begin":571,"end":576},"obj":"NNS"},{"id":"T2264","span":{"begin":559,"end":570},"obj":"VBN"},{"id":"T2265","span":{"begin":577,"end":579},"obj":"TO"},{"id":"T2266","span":{"begin":580,"end":588},"obj":"VB"},{"id":"T2267","span":{"begin":589,"end":599},"obj":"JJ"},{"id":"T2268","span":{"begin":606,"end":610},"obj":"NNS"},{"id":"T2269","span":{"begin":600,"end":605},"obj":"NN"},{"id":"T2270","span":{"begin":610,"end":611},"obj":"."},{"id":"T2271","span":{"begin":611,"end":772},"obj":"sentence"},{"id":"T2272","span":{"begin":612,"end":614},"obj":"IN"},{"id":"T2273","span":{"begin":647,"end":654},"obj":"VBP"},{"id":"T2274","span":{"begin":615,"end":618},"obj":"DT"},{"id":"T2275","span":{"begin":619,"end":624},"obj":"NN"},{"id":"T2276","span":{"begin":625,"end":627},"obj":"IN"},{"id":"T2277","span":{"begin":628,"end":634},"obj":"NNP"},{"id":"T2278","span":{"begin":635,"end":637},"obj":"FW"},{"id":"T2279","span":{"begin":638,"end":640},"obj":"FW"},{"id":"T2280","span":{"begin":640,"end":642},"obj":","},{"id":"T2281","span":{"begin":642,"end":646},"obj":"NNS"},{"id":"T2282","span":{"begin":655,"end":663},"obj":"NN"},{"id":"T2283","span":{"begin":664,"end":667},"obj":"IN"},{"id":"T2284","span":{"begin":668,"end":669},"obj":"DT"},{"id":"T2285","span":{"begin":680,"end":689},"obj":"NN"},{"id":"T2286","span":{"begin":670,"end":679},"obj":"JJ"},{"id":"T2287","span":{"begin":690,"end":692},"obj":"IN"},{"id":"T2288","span":{"begin":693,"end":696},"obj":"DT"},{"id":"T2289","span":{"begin":715,"end":721},"obj":"NN"},{"id":"T2290","span":{"begin":697,"end":705},"obj":"JJ"},{"id":"T2291","span":{"begin":706,"end":714},"obj":"JJ"},{"id":"T2292","span":{"begin":722,"end":732},"obj":"VBN"},{"id":"T2293","span":{"begin":733,"end":737},"obj":"IN"},{"id":"T2294","span":{"begin":738,"end":748},"obj":"NN"},{"id":"T2295","span":{"begin":749,"end":751},"obj":"IN"},{"id":"T2296","span":{"begin":752,"end":753},"obj":"DT"},{"id":"T2297","span":{"begin":765,"end":771},"obj":"NN"},{"id":"T2298","span":{"begin":754,"end":760},"obj":"JJ"},{"id":"T2299","span":{"begin":761,"end":764},"obj":"NN"},{"id":"T2300","span":{"begin":771,"end":772},"obj":"."},{"id":"T2301","span":{"begin":772,"end":944},"obj":"sentence"},{"id":"T2302","span":{"begin":773,"end":776},"obj":"NN"},{"id":"T2303","span":{"begin":785,"end":791},"obj":"NNS"},{"id":"T2304","span":{"begin":777,"end":784},"obj":"NN"},{"id":"T2305","span":{"begin":829,"end":833},"obj":"VBN"},{"id":"T2306","span":{"begin":792,"end":795},"obj":"CC"},{"id":"T2307","span":{"begin":796,"end":804},"obj":"NN"},{"id":"T2308","span":{"begin":805,"end":815},"obj":"NN"},{"id":"T2309","span":{"begin":816,"end":823},"obj":"NNS"},{"id":"T2310","span":{"begin":824,"end":828},"obj":"VBD"},{"id":"T2311","span":{"begin":834,"end":836},"obj":"TO"},{"id":"T2312","span":{"begin":837,"end":841},"obj":"VB"},{"id":"T2313","span":{"begin":842,"end":846},"obj":"IN"},{"id":"T2314","span":{"begin":862,"end":872},"obj":"VBZ"},{"id":"T2315","span":{"begin":847,"end":848},"obj":"DT"},{"id":"T2316","span":{"begin":854,"end":861},"obj":"NN"},{"id":"T2317","span":{"begin":849,"end":853},"obj":"NN"},{"id":"T2318","span":{"begin":873,"end":877},"obj":"IN"},{"id":"T2319","span":{"begin":878,"end":881},"obj":"DT"},{"id":"T2320","span":{"begin":882,"end":889},"obj":"NN"},{"id":"T2321","span":{"begin":890,"end":892},"obj":"IN"},{"id":"T2322","span":{"begin":893,"end":898},"obj":"NN"},{"id":"T2323","span":{"begin":899,"end":901},"obj":"TO"},{"id":"T2324","span":{"begin":902,"end":910},"obj":"VB"},{"id":"T2325","span":{"begin":911,"end":915},"obj":"IN"},{"id":"T2326","span":{"begin":916,"end":926},"obj":"JJ"},{"id":"T2327","span":{"begin":927,"end":936},"obj":"NNS"},{"id":"T2328","span":{"begin":937,"end":939},"obj":"IN"},{"id":"T2329","span":{"begin":940,"end":943},"obj":"NN"},{"id":"T2330","span":{"begin":943,"end":944},"obj":"."},{"id":"T2331","span":{"begin":944,"end":1015},"obj":"sentence"},{"id":"T2332","span":{"begin":945,"end":946},"obj":"DT"},{"id":"T2333","span":{"begin":952,"end":959},"obj":"NN"},{"id":"T2334","span":{"begin":947,"end":951},"obj":"NN"},{"id":"T2335","span":{"begin":969,"end":977},"obj":"VBN"},{"id":"T2336","span":{"begin":960,"end":963},"obj":"VBD"},{"id":"T2337","span":{"begin":964,"end":968},"obj":"RB"},{"id":"T2338","span":{"begin":978,"end":980},"obj":"TO"},{"id":"T2339","span":{"begin":981,"end":988},"obj":"VB"},{"id":"T2340","span":{"begin":989,"end":992},"obj":"DT"},{"id":"T2341","span":{"begin":1003,"end":1014},"obj":"NN"},{"id":"T2342","span":{"begin":993,"end":998},"obj":"NN"},{"id":"T2343","span":{"begin":999,"end":1002},"obj":"NN"},{"id":"T2344","span":{"begin":998,"end":999},"obj":"HYPH"},{"id":"T2345","span":{"begin":1014,"end":1015},"obj":"."},{"id":"T2346","span":{"begin":1015,"end":1164},"obj":"sentence"},{"id":"T2347","span":{"begin":1016,"end":1021},"obj":"DT"},{"id":"T2348","span":{"begin":1022,"end":1033},"obj":"NNS"},{"id":"T2349","span":{"begin":1034,"end":1039},"obj":"VBP"},{"id":"T2350","span":{"begin":1040,"end":1043},"obj":"DT"},{"id":"T2351","span":{"begin":1044,"end":1055},"obj":"NN"},{"id":"T2352","span":{"begin":1056,"end":1060},"obj":"IN"},{"id":"T2353","span":{"begin":1097,"end":1100},"obj":"VB"},{"id":"T2354","span":{"begin":1061,"end":1064},"obj":"DT"},{"id":"T2355","span":{"begin":1083,"end":1090},"obj":"NN"},{"id":"T2356","span":{"begin":1065,"end":1069},"obj":"JJ"},{"id":"T2357","span":{"begin":1070,"end":1076},"obj":"NN"},{"id":"T2358","span":{"begin":1069,"end":1070},"obj":"HYPH"},{"id":"T2359","span":{"begin":1077,"end":1082},"obj":"NN"},{"id":"T2360","span":{"begin":1091,"end":1096},"obj":"MD"},{"id":"T2361","span":{"begin":1101,"end":1103},"obj":"IN"},{"id":"T2362","span":{"begin":1104,"end":1105},"obj":"DT"},{"id":"T2363","span":{"begin":1115,"end":1124},"obj":"NN"},{"id":"T2364","span":{"begin":1106,"end":1114},"obj":"JJ"},{"id":"T2365","span":{"begin":1125,"end":1127},"obj":"IN"},{"id":"T2366","span":{"begin":1128,"end":1141},"obj":"JJ"},{"id":"T2367","span":{"begin":1152,"end":1163},"obj":"NN"},{"id":"T2368","span":{"begin":1142,"end":1147},"obj":"NN"},{"id":"T2369","span":{"begin":1148,"end":1151},"obj":"NN"},{"id":"T2370","span":{"begin":1147,"end":1148},"obj":"HYPH"},{"id":"T2371","span":{"begin":1163,"end":1164},"obj":"."}],"relations":[{"id":"R1262","pred":"det","subj":"T2154","obj":"T2155"},{"id":"R1263","pred":"nsubj","subj":"T2155","obj":"T2157"},{"id":"R1264","pred":"amod","subj":"T2156","obj":"T2155"},{"id":"R1265","pred":"acl","subj":"T2158","obj":"T2155"},{"id":"R1266","pred":"dobj","subj":"T2159","obj":"T2158"},{"id":"R1267","pred":"punct","subj":"T2160","obj":"T2155"},{"id":"R1268","pred":"prep","subj":"T2161","obj":"T2155"},{"id":"R1269","pred":"pobj","subj":"T2162","obj":"T2161"},{"id":"R1270","pred":"advmod","subj":"T2163","obj":"T2164"},{"id":"R1271","pred":"advmod","subj":"T2164","obj":"T2162"},{"id":"R1272","pred":"punct","subj":"T2165","obj":"T2166"},{"id":"R1273","pred":"parataxis","subj":"T2166","obj":"T2155"},{"id":"R1274","pred":"punct","subj":"T2167","obj":"T2166"},{"id":"R1275","pred":"punct","subj":"T2168","obj":"T2157"},{"id":"R1276","pred":"det","subj":"T2169","obj":"T2170"},{"id":"R1277","pred":"attr","subj":"T2170","obj":"T2157"},{"id":"R1278","pred":"amod","subj":"T2171","obj":"T2170"},{"id":"R1279","pred":"prep","subj":"T2172","obj":"T2170"},{"id":"R1280","pred":"det","subj":"T2173","obj":"T2174"},{"id":"R1281","pred":"pobj","subj":"T2174","obj":"T2172"},{"id":"R1282","pred":"prep","subj":"T2175","obj":"T2174"},{"id":"R1283","pred":"det","subj":"T2176","obj":"T2177"},{"id":"R1284","pred":"pobj","subj":"T2177","obj":"T2175"},{"id":"R1285","pred":"amod","subj":"T2178","obj":"T2177"},{"id":"R1286","pred":"compound","subj":"T2179","obj":"T2177"},{"id":"R1287","pred":"cc","subj":"T2180","obj":"T2177"},{"id":"R1288","pred":"det","subj":"T2181","obj":"T2182"},{"id":"R1289","pred":"conj","subj":"T2182","obj":"T2177"},{"id":"R1290","pred":"acl","subj":"T2183","obj":"T2182"},{"id":"R1291","pred":"prep","subj":"T2184","obj":"T2183"},{"id":"R1292","pred":"pobj","subj":"T2185","obj":"T2184"},{"id":"R1293","pred":"prep","subj":"T2186","obj":"T2185"},{"id":"R1294","pred":"det","subj":"T2187","obj":"T2188"},{"id":"R1295","pred":"pobj","subj":"T2188","obj":"T2186"},{"id":"R1296","pred":"nummod","subj":"T2189","obj":"T2188"},{"id":"R1297","pred":"compound","subj":"T2190","obj":"T2188"},{"id":"R1298","pred":"prep","subj":"T2191","obj":"T2188"},{"id":"R1299","pred":"amod","subj":"T2192","obj":"T2193"},{"id":"R1300","pred":"pobj","subj":"T2193","obj":"T2191"},{"id":"R1301","pred":"punct","subj":"T2194","obj":"T2157"},{"id":"R1302","pred":"nsubjpass","subj":"T2196","obj":"T2197"},{"id":"R1303","pred":"prep","subj":"T2198","obj":"T2196"},{"id":"R1304","pred":"det","subj":"T2199","obj":"T2200"},{"id":"R1305","pred":"pobj","subj":"T2200","obj":"T2198"},{"id":"R1306","pred":"amod","subj":"T2201","obj":"T2200"},{"id":"R1307","pred":"compound","subj":"T2202","obj":"T2200"},{"id":"R1308","pred":"punct","subj":"T2203","obj":"T2200"},{"id":"R1309","pred":"appos","subj":"T2204","obj":"T2200"},{"id":"R1310","pred":"punct","subj":"T2205","obj":"T2197"},{"id":"R1311","pred":"aux","subj":"T2206","obj":"T2197"},{"id":"R1312","pred":"advmod","subj":"T2207","obj":"T2197"},{"id":"R1313","pred":"auxpass","subj":"T2208","obj":"T2197"},{"id":"R1314","pred":"aux","subj":"T2209","obj":"T2210"},{"id":"R1315","pred":"xcomp","subj":"T2210","obj":"T2197"},{"id":"R1316","pred":"prep","subj":"T2211","obj":"T2210"},{"id":"R1317","pred":"det","subj":"T2212","obj":"T2213"},{"id":"R1318","pred":"pobj","subj":"T2213","obj":"T2211"},{"id":"R1319","pred":"prep","subj":"T2214","obj":"T2213"},{"id":"R1320","pred":"compound","subj":"T2215","obj":"T2216"},{"id":"R1321","pred":"pobj","subj":"T2216","obj":"T2214"},{"id":"R1322","pred":"agent","subj":"T2217","obj":"T2197"},{"id":"R1323","pred":"pobj","subj":"T2218","obj":"T2217"},{"id":"R1324","pred":"advmod","subj":"T2219","obj":"T2220"},{"id":"R1325","pred":"advmod","subj":"T2220","obj":"T2218"},{"id":"R1326","pred":"punct","subj":"T2221","obj":"T2222"},{"id":"R1327","pred":"parataxis","subj":"T2222","obj":"T2197"},{"id":"R1328","pred":"punct","subj":"T2223","obj":"T2222"},{"id":"R1329","pred":"punct","subj":"T2224","obj":"T2197"},{"id":"R1330","pred":"det","subj":"T2226","obj":"T2227"},{"id":"R1331","pred":"nsubj","subj":"T2227","obj":"T2228"},{"id":"R1332","pred":"mark","subj":"T2229","obj":"T2230"},{"id":"R1333","pred":"ccomp","subj":"T2230","obj":"T2228"},{"id":"R1334","pred":"nsubj","subj":"T2231","obj":"T2230"},{"id":"R1335","pred":"prep","subj":"T2232","obj":"T2231"},{"id":"R1336","pred":"pobj","subj":"T2233","obj":"T2232"},{"id":"R1337","pred":"acl","subj":"T2234","obj":"T2233"},{"id":"R1338","pred":"det","subj":"T2235","obj":"T2236"},{"id":"R1339","pred":"dobj","subj":"T2236","obj":"T2234"},{"id":"R1340","pred":"compound","subj":"T2237","obj":"T2236"},{"id":"R1341","pred":"prep","subj":"T2238","obj":"T2236"},{"id":"R1342","pred":"nmod","subj":"T2239","obj":"T2240"},{"id":"R1343","pred":"pobj","subj":"T2240","obj":"T2238"},{"id":"R1344","pred":"punct","subj":"T2241","obj":"T2239"},{"id":"R1345","pred":"nummod","subj":"T2242","obj":"T2239"},{"id":"R1346","pred":"det","subj":"T2243","obj":"T2244"},{"id":"R1347","pred":"dobj","subj":"T2244","obj":"T2230"},{"id":"R1348","pred":"prep","subj":"T2245","obj":"T2244"},{"id":"R1349","pred":"pobj","subj":"T2246","obj":"T2245"},{"id":"R1350","pred":"cc","subj":"T2247","obj":"T2230"},{"id":"R1351","pred":"conj","subj":"T2248","obj":"T2230"},{"id":"R1352","pred":"preconj","subj":"T2249","obj":"T2250"},{"id":"R1353","pred":"dobj","subj":"T2250","obj":"T2248"},{"id":"R1354","pred":"det","subj":"T2251","obj":"T2250"},{"id":"R1355","pred":"compound","subj":"T2252","obj":"T2253"},{"id":"R1356","pred":"compound","subj":"T2253","obj":"T2250"},{"id":"R1357","pred":"punct","subj":"T2254","obj":"T2253"},{"id":"R1358","pred":"acl","subj":"T2255","obj":"T2250"},{"id":"R1359","pred":"prep","subj":"T2256","obj":"T2255"},{"id":"R1360","pred":"pobj","subj":"T2257","obj":"T2256"},{"id":"R1361","pred":"cc","subj":"T2258","obj":"T2250"},{"id":"R1362","pred":"det","subj":"T2259","obj":"T2260"},{"id":"R1363","pred":"conj","subj":"T2260","obj":"T2250"},{"id":"R1364","pred":"prep","subj":"T2261","obj":"T2260"},{"id":"R1365","pred":"det","subj":"T2262","obj":"T2263"},{"id":"R1366","pred":"pobj","subj":"T2263","obj":"T2261"},{"id":"R1367","pred":"amod","subj":"T2264","obj":"T2263"},{"id":"R1368","pred":"aux","subj":"T2265","obj":"T2266"},{"id":"R1369","pred":"acl","subj":"T2266","obj":"T2260"},{"id":"R1370","pred":"amod","subj":"T2267","obj":"T2268"},{"id":"R1371","pred":"dobj","subj":"T2268","obj":"T2266"},{"id":"R1372","pred":"compound","subj":"T2269","obj":"T2268"},{"id":"R1373","pred":"punct","subj":"T2270","obj":"T2228"},{"id":"R1374","pred":"prep","subj":"T2272","obj":"T2273"},{"id":"R1375","pred":"det","subj":"T2274","obj":"T2275"},{"id":"R1376","pred":"pobj","subj":"T2275","obj":"T2272"},{"id":"R1377","pred":"prep","subj":"T2276","obj":"T2275"},{"id":"R1378","pred":"pobj","subj":"T2277","obj":"T2276"},{"id":"R1379","pred":"advmod","subj":"T2278","obj":"T2279"},{"id":"R1380","pred":"advmod","subj":"T2279","obj":"T2277"},{"id":"R1381","pred":"punct","subj":"T2280","obj":"T2273"},{"id":"R1382","pred":"nsubj","subj":"T2281","obj":"T2273"},{"id":"R1383","pred":"dobj","subj":"T2282","obj":"T2273"},{"id":"R1384","pred":"prep","subj":"T2283","obj":"T2282"},{"id":"R1385","pred":"det","subj":"T2284","obj":"T2285"},{"id":"R1386","pred":"pobj","subj":"T2285","obj":"T2283"},{"id":"R1387","pred":"amod","subj":"T2286","obj":"T2285"},{"id":"R1388","pred":"prep","subj":"T2287","obj":"T2285"},{"id":"R1389","pred":"det","subj":"T2288","obj":"T2289"},{"id":"R1390","pred":"pobj","subj":"T2289","obj":"T2287"},{"id":"R1391","pred":"amod","subj":"T2290","obj":"T2289"},{"id":"R1392","pred":"amod","subj":"T2291","obj":"T2289"},{"id":"R1393","pred":"acl","subj":"T2292","obj":"T2289"},{"id":"R1394","pred":"prep","subj":"T2293","obj":"T2292"},{"id":"R1395","pred":"pobj","subj":"T2294","obj":"T2293"},{"id":"R1396","pred":"prep","subj":"T2295","obj":"T2294"},{"id":"R1397","pred":"det","subj":"T2296","obj":"T2297"},{"id":"R1398","pred":"pobj","subj":"T2297","obj":"T2295"},{"id":"R1399","pred":"amod","subj":"T2298","obj":"T2297"},{"id":"R1400","pred":"compound","subj":"T2299","obj":"T2297"},{"id":"R1401","pred":"punct","subj":"T2300","obj":"T2273"},{"id":"R1402","pred":"compound","subj":"T2302","obj":"T2303"},{"id":"R1403","pred":"nsubjpass","subj":"T2303","obj":"T2305"},{"id":"R1404","pred":"compound","subj":"T2304","obj":"T2303"},{"id":"R1405","pred":"cc","subj":"T2306","obj":"T2303"},{"id":"R1406","pred":"compound","subj":"T2307","obj":"T2308"},{"id":"R1407","pred":"compound","subj":"T2308","obj":"T2309"},{"id":"R1408","pred":"conj","subj":"T2309","obj":"T2303"},{"id":"R1409","pred":"auxpass","subj":"T2310","obj":"T2305"},{"id":"R1410","pred":"aux","subj":"T2311","obj":"T2312"},{"id":"R1411","pred":"advcl","subj":"T2312","obj":"T2305"},{"id":"R1412","pred":"mark","subj":"T2313","obj":"T2314"},{"id":"R1413","pred":"ccomp","subj":"T2314","obj":"T2312"},{"id":"R1414","pred":"det","subj":"T2315","obj":"T2316"},{"id":"R1415","pred":"nsubj","subj":"T2316","obj":"T2314"},{"id":"R1416","pred":"compound","subj":"T2317","obj":"T2316"},{"id":"R1417","pred":"prep","subj":"T2318","obj":"T2314"},{"id":"R1418","pred":"det","subj":"T2319","obj":"T2320"},{"id":"R1419","pred":"pobj","subj":"T2320","obj":"T2318"},{"id":"R1420","pred":"prep","subj":"T2321","obj":"T2320"},{"id":"R1421","pred":"pobj","subj":"T2322","obj":"T2321"},{"id":"R1422","pred":"aux","subj":"T2323","obj":"T2324"},{"id":"R1423","pred":"acl","subj":"T2324","obj":"T2320"},{"id":"R1424","pred":"prep","subj":"T2325","obj":"T2324"},{"id":"R1425","pred":"amod","subj":"T2326","obj":"T2327"},{"id":"R1426","pred":"pobj","subj":"T2327","obj":"T2325"},{"id":"R1427","pred":"prep","subj":"T2328","obj":"T2324"},{"id":"R1428","pred":"pobj","subj":"T2329","obj":"T2328"},{"id":"R1429","pred":"punct","subj":"T2330","obj":"T2305"},{"id":"R1430","pred":"det","subj":"T2332","obj":"T2333"},{"id":"R1431","pred":"nsubjpass","subj":"T2333","obj":"T2335"},{"id":"R1432","pred":"compound","subj":"T2334","obj":"T2333"},{"id":"R1433","pred":"auxpass","subj":"T2336","obj":"T2335"},{"id":"R1434","pred":"advmod","subj":"T2337","obj":"T2335"},{"id":"R1435","pred":"aux","subj":"T2338","obj":"T2339"},{"id":"R1436","pred":"xcomp","subj":"T2339","obj":"T2335"},{"id":"R1437","pred":"det","subj":"T2340","obj":"T2341"},{"id":"R1438","pred":"dobj","subj":"T2341","obj":"T2339"},{"id":"R1439","pred":"compound","subj":"T2342","obj":"T2343"},{"id":"R1440","pred":"compound","subj":"T2343","obj":"T2341"},{"id":"R1441","pred":"punct","subj":"T2344","obj":"T2343"},{"id":"R1442","pred":"punct","subj":"T2345","obj":"T2335"},{"id":"R1443","pred":"det","subj":"T2347","obj":"T2348"},{"id":"R1444","pred":"nsubj","subj":"T2348","obj":"T2349"},{"id":"R1445","pred":"det","subj":"T2350","obj":"T2351"},{"id":"R1446","pred":"dobj","subj":"T2351","obj":"T2349"},{"id":"R1447","pred":"mark","subj":"T2352","obj":"T2353"},{"id":"R1448","pred":"acl","subj":"T2353","obj":"T2351"},{"id":"R1449","pred":"det","subj":"T2354","obj":"T2355"},{"id":"R1450","pred":"nsubj","subj":"T2355","obj":"T2353"},{"id":"R1451","pred":"amod","subj":"T2356","obj":"T2357"},{"id":"R1452","pred":"compound","subj":"T2357","obj":"T2355"},{"id":"R1453","pred":"punct","subj":"T2358","obj":"T2357"},{"id":"R1454","pred":"compound","subj":"T2359","obj":"T2355"},{"id":"R1455","pred":"aux","subj":"T2360","obj":"T2353"},{"id":"R1456","pred":"prep","subj":"T2361","obj":"T2353"},{"id":"R1457","pred":"det","subj":"T2362","obj":"T2363"},{"id":"R1458","pred":"pobj","subj":"T2363","obj":"T2361"},{"id":"R1459","pred":"amod","subj":"T2364","obj":"T2363"},{"id":"R1460","pred":"prep","subj":"T2365","obj":"T2363"},{"id":"R1461","pred":"amod","subj":"T2366","obj":"T2367"},{"id":"R1462","pred":"pobj","subj":"T2367","obj":"T2365"},{"id":"R1463","pred":"compound","subj":"T2368","obj":"T2369"},{"id":"R1464","pred":"compound","subj":"T2369","obj":"T2367"},{"id":"R1465","pred":"punct","subj":"T2370","obj":"T2369"},{"id":"R1466","pred":"punct","subj":"T2371","obj":"T2349"}],"text":"The second paper providing evidence, by Davies et al [26], is a biochemical study of the interaction between the homologous recombinase RAD51 and a peptide consisting of one of the eight BRC repeats from human BRCA2. Expression of a single BRC repeat (BRC4) had previously been reported to act as an inhibitor of DNA repair by Chen et al [27]. These investigators showed that expression of constructs containing the BRC4 repeat in MCF-7 cells enhances the radiosensitivity of cells and blocks both the G2/M delay associated with damage and the ability of the transfected cells to assembly subnuclear RAD51 foci. In the paper by Davies et al, data provide evidence for a plausible mechanism of the dominant negative effect associated with expression of a single BRC repeat. DNA binding assays and electron microscopy methods were used to show that a BRC3 peptide interferes with the ability of RAD51 to assemble into oligomeric filaments on DNA. A BRC4 peptide was also reported to inhibit the RAD51–DNA interaction. These experiments raise the possibility that the full-length BRCA2 protein could act as a negative regulator of inappropriate RAD51–DNA interaction."}