PMC:1382200 / 39806-40946 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/1382200","sourcedb":"PMC","sourceid":"1382200","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1382200","text":"Authors' contributions\nDEC generated the Capn2 targeting construct. PD and PAG carried out the ES electroporation and selection of targeted clones. KW performed aggregation chimeras to establish germline chimeric animals. TDV established and maintained the knockout mouse colony and performed genotyping analysis. PD, JSCA, JSE, DEC and PAG conceived the study and helped draft the manuscript. All authors read and approved the final manuscript.\nFigure 3 Genotyping of weanlings from heterozygote intercrosses. A representative example of the Southern blot genotyping of progeny from a Capn2+/- intercross is shown. Genomic DNA was extracted from mouse tail biopsies taken from three-week old weanlings and genotyped by Southern blotting as described in Figure 1. A 3.5-kb BamHI fragment from the wild-type allele was detected in all animals while a 5.3-kb BamHI fragment from the mutant allele was observed in a subset of progeny. No Capn2-/- offspring were detected among weanlings or post-implantation embryos, nor were embryo resorption sites observed. These results indicate that Capn2-/- embryos perished at a pre-implantation stage.","divisions":[{"label":"title","span":{"begin":0,"end":22}},{"label":"p","span":{"begin":23,"end":445}},{"label":"label","span":{"begin":446,"end":454}}],"tracks":[]}