Acknowledgements These studies and manuscript preparation were supported by grants from the National Cancer Institute (NCI), including CA85147 and CA 95806, and the George Isaac Cancer Research Fund. StaRT-PCR reagents for measurement of some of the genes were provided at no cost by Gene Express, Inc. Neither the NCI nor Gene Express, Inc. had any role in study design, collection, analysis, or interpretation of data, writing of the manuscript, or in the decision to submit the manuscript for publication. Figures and Tables Figure 1 Correlation of each transcription factor with XRCC1, ERCC5, GSTP1, SOD1, or GPX1. (a-f) Each panel presents the correlation coefficients (r values) for one transcription factor in relation to each of the five genes: (a) CEBPB, (b) CEBPG, (c) E2F1, (d) E2F3, (e) E2F6, (f) EVI1. The p value for each significant correlation is provided above the bar. For CEBPG, presented in panel b, the difference in r value between non-BC individuals and BC individuals was significant or nearly significant for each correlated gene, and the p value for each comparison is provided below the corresponding pair of bars. Figure 2 Scatter plot representation of bivariate correlation of CEBPG with XRCC1. (a, b) CEBPG/XRCC1 data from Figure 1b presented as scatter plots: (a) non-BC individuals, (b) BC individuals. Table 1 Demographic data of patients from whom the NBEC samples were obtained. Subject # Group Age Gender Histology Smoking Hx 1 Ethnicity 63 NBCI2 77 M 75 W3 64 NBCI 47 F 45 W 136 NBCI 38 M 25 A.A.4 139 NBCI 44 F 17.5 W 150 NBCI 70 F 45 W 156 NBCI 46 F NS5 A.A. 157 NBCI 60 M >100 W 194 NBCI 57 M 3 W 210 NBCI 40 M 34 W 257 NBCI 69 F 20 W 261 NBCI 73 F NS W 282 NBCI 83 F 60 W 285 NBCI 69 F NS W 296 NBCI 43 F 20 W 305 NBCI 50 F 40 W 315 NBCI 64 M N/A6 W 330 NBCI 39 M NS W 331 NBCI N/A N/A N/A N/A 334 NBCI 51 M >50 W 336 NBCI 31 F NS W 337 NBCI 32 M 22 N/A 339 NBCI 59 M 50 W 361 NBCI 73 F NS H7 363 NBCI 50 M 20 A.A. 34 BCI8 80 M NSCLC9 40 W 71 BCI 63 M NSCLC 100 W 85 BCI 73 F SQ10 >100 W 88 BCI 85 M SQ 75 W 99 BCI 63 M NSCLC 45 W 118 BCI 72 M SQ 30 W 146 BCI 64 F SCLC11 45 W 147 BCI 76 M SCLC 75 W 158 BCI 88 M SCLC 115.5 W 167 BCI 60 F NSCLC 50 W 171 BCI 67 M SCLC 100 W 191 BCI 75 M SQ 54 W 211 BCI 71 M SQ 50 W 212 BCI 65 M SQ 67.5 W 247 BCI 75 F SQ 50 W 255 BCI 60 F NSCLC 30 W 259 BCI 68 M CS12 137.5 W 271 BCI 58 M AC13 94.5 W 287 BCI 65 F NSCLC 50 W 300 BCI 56 M SQ 34 W 306 BCI 46 M SQ 30 W 314 BCI 69 F BC14 NS W 329 BCI 76 F PD15 >37.5 W 335 BCI 75 M SCLC 58 A.A. B3 BCI 63 M SQ 60 W 1Pack years; 2Non-bronchogenic carcinoma individual; 3White; 4African-American; 5Non-smoker; 6Not available; 7Hispanic; 8Bronchogenic carcinoma individual; 9Non-small cell lung cancer; 10Squamous carcinoma; 11Small cell lung cancer; 12Carcinoma-in-situ; 13Adenocarcinoma;14Bronchogenic Cancer, histology not specified; 15Poorly differentiated carcinoma. Table 2 Sequence for each primer used for StaRT-PCR virtually-multiplexed transcript abundance measurement or for internal standard preparation (CT) [15]. Gene Accession # Primer Sequence Position Product ACTB X00351 Forward 5' ATC CTC ACC CTG AAG TAC CC 3' 231 Reverse 5' CCA TCT CTT GCT CGA AGT CC 3' 704 493 bp CT 5' CCA TCT CTT GCT CGA AGT CCG CCA GCC AGG TCC AGA CGC A 3' 568 377 bp CAT X04076 Forward 5' CCA GAA GAA AGC GGT CAA GA 3' 1492 Reverse 5' AAC CTT CAT TTT CCC CTG GG 3' 1822 350 bp CT 5' AAC CTT CAT TTT CCC CTG GGC CAG TGA TGA GCG GGT TAC A 3' 1699 247 bp CEBPB NM_005194 Forward 5' TGT CCA AAC CAA CCG CAC AT 3' 1412 Reverse 5' AGC AAC AAG CCC GTA GGA AC 3' 1657 265 bp CT 5' AGC AAC AAG CCC GTA GGA ACA CGC GTT CAG CCA TGT TTA A 3' 1571 199 bp CEBPG U20240 Forward 5' CGG TTG AAA AGC AAG CAG AAA GCA 3' 488 Reverse 5' GAT CCC AGA AAA TAG CCT CCA ATG 3' 814 350 bp CT 5' GAT CCC AGA AAA TAG CCT CCA ATG AAC ATT CAA GCC ACA AGC TC 3' 726 282 bp E2F1 M96577 Forward 5' TGA TAC CCC AAC TCC CTC TA 3' 2076 Reverse 5' AAA GCA GGA GGG AAC AGA GC 3' 2452 396 bp CT 5' AAA GCA GGA GGG AAC AGA GCA CTG CAG GGA CCA CAG G 3' 2363 327 bp E2F3 Y10479 Forward 5' TGA AAG CCC CTC CAG AAA CAA G 3' 1019 Reverse 5' GCA GCA GGG GAG GCA GTA AGT T 3' 1336 339 bp CT 5' GCA GCA GGG GAG GCA GTA AGT TGG GGA GGC CAG AGG AGA AAG GT 3' 1253 278 bp E2F6 AF059292 Forward 5' GGG CCT GCT GCC ATC AAA AAT A 3' 99 Reverse 5' CCG CTT TCG GAC TCC CAG TTT 3' 283 205 bp CT 5' CCG CTT TCG GAC TCC CAG TTA GCG ATA CAT CAA AAC GAG G 3' 184 125 bp ERCC1 M13194 Forward 5' CTG GAG CCC CGA GGA AGC 3' 739 Reverse 5' CAC TGG GGG TTT CCT TTG 3' 1049 328 bp CT 5' CAC TGG GGG TTT CCT TGG AAG GCC AGA TCT TCT CTT 3 928 240 bp ERCC2 X52221 Forward 5' GGC CTT CTT CAC CAG CTA C 3' 1608 Reverse 5' GTA GTC CGT CTT GCC CCT G 3' 2004 415 bp CT 5' GTA GTC CGT CTT GCC CCT GTG GAA CTG GTC CCG CAG GT 3' 2597 346 bp ERCC4 U64315 Forward 5' AGT GCA TCT CCA TGT CCC GCT ACT A 3' 2213 Reverse 5' CGA TGT TCT TAA CGT GGT GCA TCA A 3' 2578 390 bp CT 5' CGA TGT TCT TAA CGT GGT GCA TCA ACA GGC TGT GGC TTG CTT TGT 3' 2433 265 bp ERCC5 D16305 Forward 5' AAG GAA AGA GAA AGA AGC AGC AGC CA 3' 3087 Reverse 5' CAA ACA CAG ATC TGG CGG TCA CGA GG 3' 3501 440 bp CT 5' CAA ACA CAG ATC TGG CGG TCA CGA GGA GCT TCC TTC ACT GAG TTC TGC GAA T 3' 3401 366 bp EVI1 NM_005241 Forward 5' CGC CGG ATA TCC ACG AAG A 3' 302 Reverse 5' ATG CTG AGA GCG AAT GTG C 3' 711 428 bp CT 5' ATG CTG AGA GCG AAT GTG CTT AAA TGC CTT GGG ACA CT 3' 587 323 bp GPX1 Y00433 Forward 5' CCT GGT GGT GCT CGG CTT CC 3' 522 Reverse 5' CAA TGG TCT GGA AGC GGC GG 3' 852 350 bp CT 5' CAA TGG TCT GGA AGC GGC GGA CCG GAG ACC AGG TGA TGA G 3' 757 279 bp GPX3 D16360 Forward 5' GCA GAG CCG GGG ACA AGA GAA 3' 113 Reverse 5' CTG CTC TTT CTC TCC ATT GAC 3' 471 379 bp CT 5' CTG CTC TTT CTC TCC ATT GAC GCT CTT CCT GTA GTG CAT TCA 3' 298 227 bp GSTM1,2,4,5 J03817 Forward 5' GGG ACG CTC CTG ATT ATG AC 3' 122 Reverse 5' GCA AAC CAT GGC CGC TTC CC 3' 442 340 bp CT 5' GCA AAC CAT GGC CGC TTC CCT TCT CCA AAA TGT CCA CAC G 3' 301 219 bp GSTM3 J05459 Forward 5' GTG CGA GTC GTC TAT GGT TC 3' 23 Reverse 5' AGT TGT GTG CGG AAA TCC AT 3' 342 339 bp CT 5' AGT TGT GTG CGG AAA TCC ATT GCT CTG GGT GAT CTT GTT C 3' 230 247 bp GSTP1 X08058 Forward 5' TCC GCT GCA AAT ACA TCT CC 3' 305 Reverse 5' TGT TTC CCG TTG CCA TTG AT 3' 616 331 bp CT 5' TGT TTC CCG TTG CCA TTG ATT AGG ACC TCA TGG ATC AGC A 3' 485 220 bp GSTT1 X79389 Forward 5' GCT CTA CCT GGA CCT GCT GT 3' 12 Reverse 5' GGA ACA CAG GGA ACA TCA CC 3' 351 359 bp CT 5' GGA ACA CAG GGA ACA TCA CCT AGA GCA GGA TGG CCA CAC T 3' 199 227 bp GSTZ1 U86529 Forward 5' TCA CCC CCT ACC CTA CCA TCA GC 3' 806 Reverse 5' ATT TCA GCG CGG GCA TTC TTT 3' 1267 482 bp CT 5' ATT TCA GCG CGG GCA TTC TTT CCG CAT TCT CAT CTC AGC CTC AC 3' 1161 399 bp mGST1 J03746 Forward 5' GTC GGA GCA CGG ATC TAC CAC A 3' 404 Reverse 5' TTC CTC TGC TCC CCT CCT ACC TA 3' 623 242 bp CT 5' TTC CTC TGC TCC CCT CCT ACC TAT TTT CAG CAA CCT GTA AGC C 3' 505 144 bp SOD1 X02317 Forward 5' TGA AGG TGT GGG GAA GCA TTA 3' 153 Reverse 5' TTA CAC CAC AAG CCA AAC GAC 3' 492 360 bp CT 5' TTA CAC CAC AAG CCA AAC GAC TGA TGC AAT GGT CTC CTG AGA 3' 384 273 bp XPA D14533 Forward 5' CTC GGC GAC GGC GGC TGC GGC TAC TGG AG 3' 178 Reverse 5' TGT CGG ACT TCC TTT GCT TCT TCT AAT GC 3' 629 480 bp CT 5' TGT CGG ACT TCC TTT GCT TCT TCT AAT GCT CTT TTT TCT AAA TCA CAG TCT 3' 487 360 bp XRCC1 M36089 Forward 5' CCC CTG AAG AGA CCA AAG CA 3' 1906 Reverse 5' CCA TTG AAG GCT GTG ACG TA 3' 2241 355 bp CT 5' CCA TTG AAG GCT GTG ACG TAT CAG GGA CTG GCA GAT G 3' 2142 276 bp Table 3 Virtually-multiplexed transcript abundance data. SUBJECT # GROUP CEBPB CEBPG E2F1 E2F3 E2F6 EVI1 CAT ERCC1 ERCC2 ERCC4 ERCC5 63 NBCI 7.2E+03 6.4E+02 2.7E+02 1.9E+02 3.4E+02 6.1E+01 2.0E+04 1.1E+05 3.8E+03 1.1E+02 4.3E+04 64 NBCI 7.9E+03 1.7E+03 2.0E+03 2.0E+01 1.7E+02 2.5E+01 2.5E+04 3.0E+05 3.4E+03 8.1E+01 2.0E+05 136 NBCI 6.2E+03 2.1E+02 7.0E+02 1.0E-02 5.0E+01 6.0E+01 2.9E+03 7.4E+03 5.9E+02 5.6E+01 1.9E+04 139 NBCI 4.5E+03 3.4E+03 2.3E+04 5.6E+02 1.0E+03 1.0E+03 6.1E+05 1.2E+06 2.2E+04 3.9E+03 4.6E+05 150 NBCI 8.5E+03 7.4E+02 1.6E+02 1.1E+02 4.1E+01 3.2E+02 3.5E+04 1.7E+05 5.7E+03 6.9E+02 7.2E+04 156 NBCI 2.1E+04 1.2E+03 7.5E+02 ND1 ND 1.4E+02 1.5E+04 1.8E+05 2.0E+03 2.9E+02 3.0E+04 157 NBCI 2.3E+04 4.1E+03 3.1E+03 2.1E+02 6.1E+02 1.4E+02 3.5E+05 5.6E+05 8.4E+03 1.6E+03 2.1E+05 194 NBCI 6.5E+03 2.1E+03 2.9E+02 2.6E+02 8.5E+02 4.7E+02 4.5E+04 6.1E+05 4.7E+03 4.4E+02 8.9E+04 210 NBCI 1.0E+04 2.1E+03 7.6E+02 4.0E+02 6.1E+02 3.6E+02 7.6E+04 7.6E+04 3.4E+03 7.7E+02 7.2E+04 257 NBCI 1.1E+04 1.8E+03 2.7E+02 8.9E+02 1.7E+03 9.7E+02 1.0E+05 2.6E+05 1.6E+03 7.6E+02 7.4E+04 261 NBCI 7.6E+03 1.3E+03 2.5E+02 1.7E+02 1.6E+02 9.4E+01 4.4E+04 2.7E+05 5.1E+03 4.6E+02 1.3E+05 282 NBCI 6.4E+03 1.2E+03 5.4E+02 4.1E+01 2.9E+01 1.2E+02 4.1E+04 1.0E+05 6.0E+03 8.1E+02 4.0E+04 285 NBCI 2.6E+03 4.4E+02 2.5E+03 1.1E+03 ND 1.2E+02 3.7E+04 9.1E+04 1.6E+03 7.7E+03 1.6E+04 296 NBCI 1.9E+04 1.0E+03 1.0E+03 ND 2.8E+01 ND 6.9E+04 2.5E+05 3.6E+03 1.7E+02 7.1E+04 305 NBCI 1.8E+03 9.1E+01 6.1E+02 ND 8.7E+01 2.6E+02 2.0E+04 4.9E+04 4.3E+02 2.8E+02 1.3E+04 315 NBCI 3.5E+03 1.3E+03 1.2E+03 2.0E+02 7.5E+01 6.1E+02 4.7E+04 2.1E+05 2.7E+03 2.4E+03 6.2E+04 330 NBCI 2.7E+03 2.4E+02 4.0E+02 1.1E+02 3.5E+02 4.0E+02 3.6E+04 8.3E+04 1.5E+03 1.7E+03 2.8E+04 331 NBCI 7.3E+03 1.3E+03 8.0E+02 ND ND ND 8.6E+04 2.6E+05 6.5E+02 6.4E+02 6.2E+04 334 NBCI 3.7E+03 6.1E+02 1.1E+03 2.3E+01 1.9E+01 4.0E+01 7.8E+04 1.4E+05 2.5E+03 1.3E+03 5.1E+04 336 NBCI 3.6E+03 8.4E+02 2.8E+03 4.3E+02 1.2E+02 2.5E+02 8.9E+04 2.1E+05 7.9E+03 3.1E+03 9.5E+04 337 NBCI 5.0E+03 9.3E+02 1.1E+03 1.6E+02 2.5E+02 ND 6.5E+04 1.9E+05 3.3E+03 1.3E+03 4.2E+04 339 NBCI 2.5E+03 3.2E+02 5.2E+02 3.4E+01 4.3E+01 6.3E+01 5.0E+04 8.7E+04 2.3E+03 7.7E+02 3.2E+04 361 NBCI 7.9E+03 2.5E+03 6.7E+02 5.8E+02 1.4E+03 3.5E+02 6.7E+04 2.5E+05 1.5E+03 2.8E+03 4.7E+04 363 NBCI 6.2E+03 1.7E+03 1.4E+03 1.1E+02 1.9E+02 5.2E+01 7.6E+04 1.4E+05 3.7E+03 7.3E+02 7.2E+04 34 BCI 1.9E+03 1.7E+03 5.7E+02 2.4E+02 1.2E+02 3.7E+02 6.7E+04 5.6E+05 6.1E+03 1.5E+03 1.2E+05 71 BCI 7.6E+03 1.2E+03 1.3E+03 1.7E+01 1.8E+02 2.1E+01 9.5E+04 6.7E+05 1.8E+04 2.4E+02 2.2E+05 85 BCI 1.0E+04 9.7E+02 8.7E+02 6.3E+01 ND ND 2.6E+04 4.9E+04 1.1E+03 3.9E+02 2.3E+04 88 BCI 1.5E+03 4.0E+02 4.2E+02 1.1E+02 ND ND 3.1E+04 2.9E+04 2.7E+03 3.6E+02 3.9E+04 99 BCI 1.4E+04 3.3E+03 2.6E+03 2.2E+02 3.1E+03 1.4E+02 2.1E+05 2.0E+05 3.7E+03 9.3E+02 1.4E+05 118 BCI 1.5E+03 1.2E+03 2.8E+02 ND ND 1.4E+02 2.4E+04 3.5E+04 1.9E+03 1.2E+03 1.7E+04 146 BCI 1.9E+04 1.2E+03 2.3E+02 1.2E+02 1.6E+03 6.0E+01 3.1E+04 1.4E+05 4.9E+03 2.7E+02 6.2E+04 147 BCI 2.8E+03 1.3E+03 N/A2 3.6E+02 2.8E+02 3.8E+02 6.1E+04 3.0E+05 2.7E+03 1.1E+03 1.8E+04 158 BCI 4.9E+03 8.8E+02 1.9E+02 2.8E+01 4.0E+02 6.8E+01 2.2E+04 1.4E+05 1.7E+03 1.5E+02 1.3E+04 167 BCI 6.7E+03 9.2E+02 4.2E+02 1.8E+02 8.2E+02 3.8E+02 8.9E+04 1.1E+05 2.2E+03 2.8E+02 4.1E+04 171 BCI 1.0E+04 3.9E+03 3.2E+03 5.1E+02 8.9E+01 2.4E+02 5.3E+05 8.3E+05 1.3E+04 6.3E+02 1.6E+05 191 BCI 1.6E+04 2.4E+03 1.5E+02 1.5E+02 7.1E+01 1.2E+02 2.0E+04 9.6E+04 2.5E+03 3.4E+02 1.2E+04 211 BCI 3.8E+03 1.1E+03 6.5E+02 6.3E+02 3.2E+02 2.9E+02 8.3E+04 3.5E+05 2.8E+03 3.2E+02 9.1E+04 212 BCI 1.8E+04 2.8E+03 6.0E+02 3.4E+02 2.0E+02 2.2E+02 3.4E+04 2.1E+05 5.2E+03 3.0E+02 3.6E+04 247 BCI 6.5E+03 7.5E+02 6.4E+02 1.8E+02 ND 1.2E+02 7.8E+04 4.4E+04 5.5E+02 2.7E+03 1.3E+04 255 BCI 1.9E+04 1.1E+03 6.1E+02 1.8E+01 2.3E+02 7.6E+01 1.1E+05 6.5E+05 1.2E+04 1.2E+03 3.8E+05 259 BCI 8.4E+03 6.4E+02 5.1E+02 ND 6.7E+01 4.0E+01 4.7E+04 1.5E+05 2.6E+03 3.8E+02 7.9E+04 271 BCI 4.1E+03 6.6E+02 1.4E+03 6.5E+01 2.7E+02 1.9E+02 9.1E+04 1.5E+05 1.6E+03 ND 1.5E+05 287 BCI 8.7E+03 1.1E+03 9.5E+01 7.4E+01 4.0E+01 2.6E+02 4.7E+04 1.2E+05 6.5E+03 1.1E+02 4.1E+04 300 BCI 4.9E+03 4.4E+02 4.1E+02 4.4E+01 ND 5.1E+01 3.4E+04 6.9E+04 1.0E+03 1.0E+03 2.6E+04 306 BCI 6.5E+03 6.8E+02 4.9E+02 4.4E+01 ND 1.7E+01 3.7E+04 2.5E+05 2.8E+03 3.2E+02 4.1E+04 314 BCI 4.4E+03 9.3E+02 4.8E+02 3.1E+02 ND 1.9E+02 5.3E+04 6.2E+04 4.5E+03 5.2E+02 3.1E+04 329 BCI 1.4E+04 3.5E+02 2.4E+02 ND ND ND 7.9E+04 1.5E+05 1.4E+03 ND 1.5E+05 335 BCI 4.2E+03 3.7E+02 2.2E+03 3.3E+02 2.4E+02 2.4E+02 4.8E+04 1.9E+05 9.8E+03 3.1E+02 3.3E+04 B3 BCI 7.4E+03 9.3E+02 1.4E+02 2.4E+02 1.8E+02 3.8E+02 3.4E+04 1.6E+05 2.2E+03 2.8E+02 4.1E+04 SUBJECT # GROUP GPX1 GPX3 GSTM15 GSTM3 GSTP1 GSTT1 GSTZ1 MGST1 SOD1 XPA XRCC1 63 NBCI 8.4E+05 1.5E+03 7.3E+03 3.9E+03 1.9E+06 ND 3.1E+03 1.7E+05 1.6E+05 2.5E+03 2.1E+04 64 NBCI 4.4E+05 3.1E+02 1.5E+04 2.6E+03 3.5E+06 6.4E+03 2.0E+03 1.6E+05 6.5E+05 2.2E+03 4.2E+04 136 NBCI 2.0E+05 5.2E+02 6.3E+03 2.2E+03 5.3E+05 ND 8.9E+02 3.2E+04 5.3E+04 1.1E+03 4.4E+03 139 NBCI 1.8E+06 3.2E+03 5.2E+04 8.3E+03 3.2E+07 ND 2.8E+04 8.4E+05 2.2E+06 2.6E+04 1.6E+05 150 NBCI 2.1E+05 2.8E+03 1.1E+04 1.1E+03 1.5E+06 ND 3.1E+03 3.1E+04 2.0E+05 2.0E+03 3.1E+04 156 NBCI 4.8E+05 3.9E+03 1.9E+04 6.5E+03 2.9E+06 ND 4.9E+03 5.8E+04 1.3E+05 4.0E+03 2.5E+04 157 NBCI 2.4E+06 2.0E+03 2.6E+04 3.3E+03 1.8E+07 8.5E+03 3.6E+03 3.6E+05 1.8E+06 6.2E+03 1.7E+05 194 NBCI 5.4E+05 4.0E+03 1.2E+04 1.1E+03 7.8E+06 7.7E+03 3.2E+03 9.6E+04 5.8E+05 3.7E+03 2.4E+05 210 NBCI 3.2E+05 3.3E+03 4.1E+03 2.6E+03 3.9E+06 1.8E+03 3.7E+03 7.2E+04 3.3E+05 4.8E+03 2.9E+04 257 NBCI 6.3E+05 2.5E+03 1.1E+04 1.2E+02 2.8E+06 8.7E+03 2.9E+03 2.3E+04 1.2E+05 2.6E+03 4.9E+04 261 NBCI 7.6E+05 4.7E+03 1.6E+04 7.4E+03 3.1E+06 1.5E+03 1.8E+03 1.2E+05 4.5E+05 4.9E+03 7.2E+04 282 NBCI 4.6E+05 2.5E+03 1.3E+04 2.3E+03 1.9E+06 1.5E+04 1.3E+03 6.5E+04 4.5E+05 2.0E+03 3.1E+04 285 NBCI 4.3E+05 5.3E+03 8.4E+04 6.2E+02 3.5E+06 7.3E+03 9.2E+03 3.0E+04 2.3E+05 ND 2.0E+04 296 NBCI 8.1E+05 1.5E+03 7.4E+03 4.1E+03 3.6E+06 8.1E+03 4.5E+03 2.0E+05 5.4E+05 2.6E+03 5.2E+04 305 NBCI 1.4E+05 6.5E+02 4.0E+03 3.2E+03 1.6E+06 ND 6.9E+02 4.1E+04 1.8E+05 1.3E+03 5.7E+03 315 NBCI 4.2E+05 7.6E+03 5.1E+03 3.1E+03 7.9E+06 7.2E+03 4.3E+03 7.3E+04 4.5E+05 1.0E+04 3.8E+04 330 NBCI 1.2E+05 2.0E+03 5.8E+03 3.5E+03 1.1E+06 3.1E+01 1.3E+03 2.9E+04 2.3E+05 1.4E+03 1.7E+04 331 NBCI 6.1E+05 3.4E+03 1.3E+04 3.6E+03 7.3E+06 1.0E+04 1.8E+03 1.2E+05 1.3E+06 1.9E+03 5.7E+04 334 NBCI 6.5E+05 4.0E+03 2.7E+04 1.7E+04 3.7E+06 4.6E+03 3.0E+03 6.8E+04 5.9E+05 2.9E+03 2.6E+04 336 NBCI 4.7E+05 2.7E+03 4.4E+04 1.6E+03 3.4E+06 1.2E+04 8.3E+03 6.2E+04 5.4E+05 4.2E+03 1.2E+05 337 NBCI 2.8E+05 2.8E+03 3.8E+03 4.3E+03 1.5E+06 5.3E+03 5.6E+03 4.9E+04 3.3E+05 1.7E+03 4.4E+04 339 NBCI 3.1E+05 1.6E+04 3.0E+04 1.1E+03 3.5E+06 6.6E+03 2.0E+03 6.6E+04 2.4E+05 2.5E+03 2.2E+04 361 NBCI 3.7E+05 8.4E+02 1.5E+04 1.3E+03 7.8E+06 3.1E+03 2.7E+03 4.8E+04 6.4E+05 6.1E+03 7.7E+04 363 NBCI 6.1E+05 2.7E+03 2.2E+04 2.0E+03 8.1E+06 9.9E+03 2.4E+03 9.7E+04 7.0E+05 7.0E+03 3.6E+04 34 BCI 8.8E+05 3.1E+03 3.4E+03 5.1E+02 1.8E+06 ND 1.9E+03 6.1E+04 2.9E+05 3.1E+03 5.6E+04 71 BCI 8.8E+05 1.3E+04 3.2E+04 2.2E+03 6.7E+06 ND 2.5E+03 4.1E+05 7.5E+05 3.1E+03 5.6E+05 85 BCI 2.3E+05 1.1E+03 2.6E+04 4.1E+03 1.2E+06 1.0E+04 8.1E+02 4.8E+04 1.5E+05 3.5E+03 1.8E+04 88 BCI 1.3E+05 1.5E+03 3.5E+03 1.1E+03 6.9E+05 ND 5.6E+02 2.4E+04 1.9E+05 2.4E+03 1.6E+04 99 BCI 9.0E+05 2.1E+03 1.5E+04 5.5E+03 9.6E+06 6.7E+03 8.0E+03 1.2E+05 9.4E+05 8.5E+03 4.7E+04 118 BCI 8.0E+04 3.8E+03 1.1E+04 4.8E+03 1.7E+06 4.0E+03 4.5E+02 4.4E+04 1.7E+05 1.1E+03 6.1E+03 146 BCI 4.1E+05 2.9E+04 4.7E+04 4.7E+02 2.0E+06 7.1E+03 1.6E+04 6.6E+04 1.7E+05 3.8E+04 6.5E+04 147 BCI 4.1E+05 2.5E+03 8.2E+03 7.3E+02 8.8E+05 1.7E+03 1.3E+03 2.3E+04 6.5E+05 2.3E+02 7.4E+04 158 BCI 2.6E+04 2.6E+03 1.4E+04 4.7E+03 2.3E+06 2.9E+03 1.2E+03 2.3E+04 3.7E+04 1.9E+03 2.3E+04 167 BCI 1.9E+05 2.5E+03 5.3E+03 1.8E+03 2.4E+06 1.4E+04 2.3E+03 9.5E+04 4.0E+05 2.9E+03 2.8E+04 171 BCI 1.6E+06 1.7E+03 1.6E+04 4.0E+03 7.8E+06 6.0E+03 6.5E+03 2.5E+05 1.4E+06 1.4E+04 6.0E+04 191 BCI 4.5E+05 1.9E+03 3.5E+03 2.5E+02 1.7E+06 3.6E+03 5.6E+02 2.1E+04 1.6E+05 2.5E+03 2.1E+04 211 BCI 6.0E+05 1.7E+04 1.1E+04 1.3E+02 1.0E+07 5.5E+03 4.1E+03 2.4E+04 7.1E+05 4.3E+03 8.3E+04 212 BCI 6.5E+04 1.6E+03 9.6E+03 1.1E+03 4.0E+06 8.1E+03 9.5E+02 7.5E+04 9.4E+04 3.1E+03 3.6E+04 247 BCI 2.3E+05 2.0E+02 1.4E+04 2.1E+03 1.3E+06 7.1E+03 1.1E+03 2.4E+04 1.3E+05 1.3E+03 1.1E+04 255 BCI 1.3E+06 7.3E+03 1.5E+04 2.5E+03 2.8E+06 3.8E+04 8.1E+03 1.1E+05 2.8E+06 2.0E+03 1.0E+05 259 BCI 3.0E+06 5.9E+03 1.4E+04 7.0E+03 9.2E+06 5.1E+03 5.1E+03 1.1E+05 5.0E+05 4.8E+03 2.1E+04 271 BCI 7.4E+05 9.5E+02 3.8E+03 2.6E+03 2.4E+06 3.7E+03 3.1E+03 7.1E+04 6.3E+05 4.1E+03 3.0E+04 287 BCI 5.8E+05 8.7E+03 1.1E+04 2.3E+03 1.9E+06 1.5E+04 6.3E+02 8.8E+04 1.2E+05 4.7E+03 3.3E+04 300 BCI 2.7E+05 1.5E+03 7.7E+03 5.0E+03 3.7E+06 7.1E+03 1.9E+03 6.7E+04 3.2E+05 8.0E+02 1.3E+04 306 BCI 4.2E+05 2.0E+02 9.7E+03 2.3E+03 2.2E+06 ND 4.8E+03 5.6E+04 1.1E+06 4.5E+02 1.9E+04 314 BCI 1.6E+05 3.7E+03 1.7E+04 1.1E+03 1.2E+06 1.9E+02 1.5E+03 2.7E+04 2.7E+05 2.8E+03 3.4E+04 329 BCI 2.3E+05 8.9E+03 1.6E+04 5.3E+03 7.2E+06 7.8E+03 1.4E+03 5.7E+04 8.9E+05 1.9E+03 4.8E+04 335 BCI 4.1E+05 6.5E+03 2.1E+04 7.1E+03 4.3E+06 1.8E+02 7.0E+03 6.9E+04 5.2E+05 3.9E+03 4.4E+04 B3 BCI 4.0E+05 3.2E+03 6.0E+03 3.8E+02 1.6E+06 7.7E+03 1.8E+03 1.8E+04 2.6E+05 3.7E+03 1.7E+04 Virtually-multiplexed transcript abundance data for each gene (in the form of molecules/106 β-actin molecules) from all experiments were included in the same Standardized Expression Database (SED). These data are now directly comparable to previously published virtually-multiplexed transcript abundance data from this laboratory [15], or to Virtually-multiplexed transcript abundance data collected by others who use the NCI-funded (R24 CA 95806) Standardized Expression Measurement (SEM) Center. The data presented here represent more than 6,000 Virtually-multiplexed transcript abundance measurements conducted in multiple experiments. The sixteen antioxidant or DNA repair genes and each of the six transcription factors except for E2F1 were measured in each NBEC sample from 49 individuals (24 non-BC individuals and 25 BC individuals). 1ND, Not detectable (When 60 molecules of Competitive Template (CT) were added to the reaction, CT was detected but native template was not.) 2NA, Not available. E2F1 was measured in all of the samples except for one BC individual (24 non-BC individuals and 24 BC individuals). Table 4 Bivariate analysis of virtually-multiplexed transcript abundance data values for each antioxidant or DNA repair gene versus each transcription factor. Non-BC Individuals n = 24 BC Individuals n = 25 ALL n = 49 Antioxidant/DNA Repair Genes vs Transcription Factors r Value p Value r Value p Value r Value p Value CAT vs CEBPB 0.13 1 0.18 1 0.15 1 CAT vs CEBPG 0.65 0.004 0.35 0.48 0.55 <0.0006 CAT vs E2F1* 0.54 0.04 0.68 0.002 0.56 <0.0006 CAT vs E2F3 0.48 0.12 0.18 1 0.37 0.06 CAT vs E2F6 0.26 1 0.3 0.84 0.25 0.48 CAT vs EVI1 -0.01 1 0.21 1 0.08 1 ERCC1 vs CEBPB 0.32 0.78 0.27 1 0.29 0.24 ERCC1 vs CEBPG 0.77 <0.0006 0.42 0.24 0.62 <0.0006 ERCC1 vs E2F1 0.35 0.54 0.39 0.36 0.37 0.06 ERCC1 vs E2F3 0.39 0.36 0.21 1 0.31 0.18 ERCC1 vs E2F6 0.17 1 0.63 0.005 0.42 0.02 ERCC1 vs EVI1 -0.02 1 0.38 0.36 0.17 1 ERCC2 vs CEBPB 0.25 1 0.19 1 0.22 0.84 ERCC2 vs CEBPG 0.63 0.006 0.39 0.3 0.53 <0.0006 ERCC2 vs E2F1 0.39 0.36 0.32 0.72 0.33 0.12 ERCC2 vs E2F3 0.58 0.02 0.22 1 0.42 0.02 ERCC2 vs E2F6 0.37 0.42 0.51 0.06 0.42 0.02 ERCC2 vs EVI1 0.19 1 0.29 0.96 0.23 0.66 ERCC4 vs CEBPB -0.35 0.6 -0.11 1 -0.16 1 ERCC4 vs CEBPG 0.24 1 0.37 0.42 0.25 0.48 ERCC4 vs E2F1 0.42 0.24 0.04 1 0.2 1 ERCC4 vs E2F3 0.6 0.01 0.33 0.6 0.33 0.12 ERCC4 vs E2F6 -0.04 1 0.04 1 0.07 1 ERCC4 vs EVI1 0.24 1 0.33 0.66 0.27 0.36 ERCC5 vs CEBPB 0.4 0.3 0.28 1 0.33 0.12 ERCC5 vs CEBPG 0.79 <0.0006 0.12 1 0.46 0.005 ERCC5 vs E2F1 0.44 0.18 0.45 0.18 0.44 0.01 ERCC5 vs E2F3 0.39 0.36 -0.11 1 0.13 1 ERCC5 vs E2F6 0.41 0.3 0.35 0.54 0.38 0.04 ERCC5 vs EVI1 0.07 1 -0.04 1 0.01 1 GPX1 vs CEBPB 0.49 0.06 0.24 1 0.32 0.12 GPX1 vs CEBPG 0.72 <0.0006 0.19 1 0.4 0.02 GPX1 vs E2F1 0.48 0.12 0.38 0.36 0.43 0.02 GPX1 vs E2F3 0.22 1 -0.004 1 0.08 1 GPX1 vs E2F6 0.06 1 0.36 0.48 0.28 0.3 GPX1 vs EVI1 -0.06 1 0.2 1 0.1 1 GPX3 vs CEBPB -0.18 1 0.14 1 0.02 1 GPX3 vs CEBPG 0.13 1 0.01 1 0.07 1 GPX3 vs E2F1 -0.03 1 -0.17 1 -0.12 1 GPX3 vs E2F3 0.32 0.78 -0.2 1 0.05 1 GPX3 vs E2F6 -0.26 1 0.44 0.18 0.19 1 GPX3 vs EVI1 0.06 1 0.08 1 0.06 1 GSTM1-5 vs CEBPB -0.08 1 0.43 0.18 0.17 1 GSTM1-5 vs CEBPG 0.25 1 0.02 1 0.16 1 GSTM1-5 vs E2F1 0.51 0.06 0.23 1 0.41 0.02 GSTM1-5 vs E2F3 0.29 0.96 -0.16 1 0.1 1 GSTM1-5 vs E2F6 -0.3 0.9 0.006 1 -0.1 1 GSTM1-5 vs EVI1 0.22 1 -0.12 1 0.07 1 GSTM3 vs CEBPB 0.01 1 0.06 1 0.04 1 GSTM3 vs CEBPG -0.007 1 -0.28 1 0.01 1 GSTM3 vs E2F1 0.29 1 0.35 0.54 0.34 0.12 GSTM3 vs E2F3 -0.31 0.84 -0.49 0.06 -0.4 0.03 GSTM3 vs E2F6 -0.11 1 -0.25 1 -0.16 1 GSTM3 vs EVI1 -0.27 1 -0.25 1 -0.25 0.54 GSTP1 vs CEBPB 0.19 1 0.38 0.36 0.28 0.36 GSTP1 vs CEBPG 0.74 <0.0006 0.18 1 0.51 0.001 GSTP1 vs E2F1 0.6 0.01 0.46 0.12 0.56 <0.0006 GSTP1 vs E2F3 0.32 0.78 -0.25 1 0.07 1 GSTP1 vs E2F6 0.1 1 0.35 0.48 0.26 0.42 GSTP1 vs EVI1 0.11 1 0.13 1 0.12 1 GSTT1 vs CEBPB 0.03 1 0.45 0.12 0.24 0.6 GSTT1 vs CEBPG 0.39 0.36 0.16 1 0.3 0.24 GSTT1 vs E2F1 0.07 1 -0.15 1 -0.05 1 GSTT1 vs E2F3 0.35 0.54 -0.1 1 0.17 1 GSTT1 vs E2F6 0.05 1 0.21 1 0.11 1 GSTT1 vs EVI1 -0.26 1 0.22 1 -0.04 1 GSTZ1 vs CEBPB 0.11 1 0.36 0.42 0.25 0.54 GSTZ1 vs CEBPG 0.51 0.06 0.08 1 0.28 0.3 GSTZ1 vs E2F1 0.64 0.004 0.5 0.06 0.58 <0.0006 GSTZ1 vs E2F3 0.42 0.24 0.14 1 0.25 0.54 GSTZ1 vs E2F6 -0.05 1 0.48 0.12 0.32 0.18 GSTZ1 vs EVI1 0.02 1 0.27 1 0.16 1 mGST vs CEBPB 0.31 0.78 0.35 0.48 0.32 0.12 mGST vs CEBPG 0.56 0.02 0.25 1 0.42 0.02 mGST vs E2F1 0.58 0.02 0.54 0.04 0.58 <0.0006 mGST vs E2F3 0.03 1 -0.15 1 -0.06 1 mGST vs E2F6 0.17 1 0.29 0.96 0.27 0.36 mGST vs EVI1 -0.16 1 0.07 1 -0.04 1 SOD1 vs CEBPB 0.13 1 0.15 1 0.14 1 SOD1 vs CEBPG 0.66 0.002 0.009 1 0.36 0.06 SOD1 vs E2F1 0.59 0.02 0.55 0.04 0.56 <0.0006 SOD1 vs E2F3 0.25 1 -0.07 1 0.09 1 SOD1 vs E2F6 0.12 1 0.14 1 0.14 1 SOD1 vs EVI1 -0.17 1 0.03 1 -0.06 1 XPA vs CEBPB 0.31 0.84 0.42 0.24 0.31 0.18 XPA vs CEBPG 0.36 0.54 0.33 0.66 0.34 0.12 XPA vs E2F1 -0.05 1 0.22 1 -0.02 1 XPA vs E2F3 -0.07 1 0.14 1 -0.01 1 XPA vs E2F6 0.55 0.04 0.46 0.12 0.4 0.02 XPA vs EVI1 0.04 1 0.07 1 0.04 1 XRCC1 vs CEBPB 0.36 0.48 0.28 1 0.32 0.18 XRCC1 vs CEBPG 0.83 <0.0006 0.27 1 0.591 <0.0006 XRCC1 vs E2F1 0.32 0.78 0.37 0.48 0.35 0.12 XRCC1 vs E2F3 0.47 0.12 0.22 1 0.35 0.06 XRCC1 vs E2F6 0.26 1 0.54 0.04 0.41 0.02 XRCC1 vs EVI1 -0.009 1 0.12 1 0.06 1 The correlation of logarithmically transformed virtually-multiplexed transcript abundance data (Table 3) from each of the six transcription factors with each of the sixteen antioxidant or DNA repair genes was determined. The transformation was necessary due to the wide range of expression of each gene among the samples. The correlation coefficient (r value) and level of significance (p value) for each correlation are presented. Significance (p < 0.01) was determined using a two-tailed test following Bonferroni adjustment for six multiple comparisons (comparison of each of six transcription factors to each of the antioxidant or DNA repair genes). *values for E2F1 obtained in all but one BC individuals.