Id |
Subject |
Object |
Predicate |
Lexical cue |
T16409 |
0-4 |
NN |
denotes |
MDCK |
T16410 |
5-10 |
NNS |
denotes |
cells |
T16411 |
66-74 |
VBN |
denotes |
cultured |
T16412 |
11-12 |
-LRB- |
denotes |
( |
T16413 |
20-24 |
NN |
denotes |
ATCC |
T16414 |
12-15 |
NN |
denotes |
CCL |
T16415 |
15-16 |
HYPH |
denotes |
- |
T16416 |
16-18 |
CD |
denotes |
34 |
T16417 |
18-19 |
: |
denotes |
; |
T16418 |
24-26 |
, |
denotes |
, |
T16419 |
26-34 |
NNP |
denotes |
Manassas |
T16420 |
34-36 |
, |
denotes |
, |
T16421 |
36-44 |
NNP |
denotes |
Virginia |
T16422 |
44-46 |
, |
denotes |
, |
T16423 |
46-52 |
NNP |
denotes |
United |
T16424 |
53-59 |
NNP |
denotes |
States |
T16425 |
59-60 |
-RRB- |
denotes |
) |
T16426 |
61-65 |
VBD |
denotes |
were |
T16427 |
75-77 |
IN |
denotes |
in |
T16428 |
78-82 |
NN |
denotes |
DMEM |
T16429 |
83-84 |
-LRB- |
denotes |
( |
T16430 |
90-97 |
NNP |
denotes |
Aldrich |
T16431 |
84-89 |
NNP |
denotes |
Sigma |
T16432 |
89-90 |
HYPH |
denotes |
- |
T16433 |
97-99 |
, |
denotes |
, |
T16434 |
99-102 |
NNP |
denotes |
St. |
T16435 |
103-108 |
NNP |
denotes |
Louis |
T16436 |
108-110 |
, |
denotes |
, |
T16437 |
110-118 |
NNP |
denotes |
Missouri |
T16438 |
118-120 |
, |
denotes |
, |
T16439 |
120-126 |
NNP |
denotes |
United |
T16440 |
127-133 |
NNP |
denotes |
States |
T16441 |
133-134 |
-RRB- |
denotes |
) |
T16442 |
135-147 |
VBN |
denotes |
supplemented |
T16443 |
148-152 |
IN |
denotes |
with |
T16444 |
153-155 |
CD |
denotes |
10 |
T16445 |
155-156 |
NN |
denotes |
% |
T16446 |
157-160 |
NN |
denotes |
FBS |
T16447 |
161-162 |
-LRB- |
denotes |
( |
T16448 |
168-175 |
NNP |
denotes |
Aldrich |
T16449 |
162-167 |
NNP |
denotes |
Sigma |
T16450 |
167-168 |
HYPH |
denotes |
- |
T16451 |
175-176 |
-RRB- |
denotes |
) |
T16452 |
176-178 |
, |
denotes |
, |
T16453 |
178-181 |
CD |
denotes |
100 |
T16454 |
182-183 |
NN |
denotes |
U |
T16455 |
183-184 |
SYM |
denotes |
/ |
T16456 |
184-186 |
NN |
denotes |
ml |
T16457 |
187-189 |
IN |
denotes |
of |
T16458 |
190-200 |
NN |
denotes |
penicillin |
T16459 |
200-202 |
, |
denotes |
, |
T16460 |
202-205 |
CC |
denotes |
and |
T16461 |
206-209 |
CD |
denotes |
100 |
T16462 |
210-212 |
NN |
denotes |
μg |
T16463 |
212-213 |
SYM |
denotes |
/ |
T16464 |
213-215 |
NN |
denotes |
ml |
T16465 |
216-218 |
IN |
denotes |
of |
T16466 |
219-231 |
NN |
denotes |
streptomycin |
T16467 |
232-234 |
IN |
denotes |
at |
T16468 |
235-237 |
CD |
denotes |
37 |
T16469 |
238-240 |
NN |
denotes |
°C |
T16470 |
241-243 |
IN |
denotes |
in |
T16471 |
244-245 |
CD |
denotes |
5 |
T16472 |
245-246 |
NN |
denotes |
% |
T16473 |
247-250 |
NN |
denotes |
CO2 |
T16474 |
250-251 |
. |
denotes |
. |
T16475 |
251-491 |
sentence |
denotes |
To generate stable MDCK cell lines, cells were transfected using Lipofectamine 2000 (Invitrogen) and the pcDNA3.1 expression constructs (containing wild-type AQP2, AQP2-F204V, or no insert) and selected with 900 μg/ml G418 (Sigma-Aldrich). |
T16476 |
252-254 |
TO |
denotes |
To |
T16477 |
255-263 |
VB |
denotes |
generate |
T16478 |
299-310 |
VBN |
denotes |
transfected |
T16479 |
264-270 |
JJ |
denotes |
stable |
T16480 |
281-286 |
NNS |
denotes |
lines |
T16481 |
271-275 |
NN |
denotes |
MDCK |
T16482 |
276-280 |
NN |
denotes |
cell |
T16483 |
286-288 |
, |
denotes |
, |
T16484 |
288-293 |
NNS |
denotes |
cells |
T16485 |
294-298 |
VBD |
denotes |
were |
T16486 |
311-316 |
VBG |
denotes |
using |
T16487 |
317-330 |
NN |
denotes |
Lipofectamine |
T16488 |
331-335 |
CD |
denotes |
2000 |
T16489 |
336-337 |
-LRB- |
denotes |
( |
T16490 |
337-347 |
NNP |
denotes |
Invitrogen |
T16491 |
347-348 |
-RRB- |
denotes |
) |
T16492 |
349-352 |
CC |
denotes |
and |
T16493 |
353-356 |
DT |
denotes |
the |
T16494 |
377-387 |
NNS |
denotes |
constructs |
T16495 |
357-365 |
NN |
denotes |
pcDNA3.1 |
T16496 |
366-376 |
NN |
denotes |
expression |
T16497 |
388-389 |
-LRB- |
denotes |
( |
T16498 |
389-399 |
VBG |
denotes |
containing |
T16499 |
400-404 |
JJ |
denotes |
wild |
T16500 |
405-409 |
NN |
denotes |
type |
T16501 |
404-405 |
HYPH |
denotes |
- |
T16502 |
410-414 |
NN |
denotes |
AQP2 |
T16503 |
414-416 |
, |
denotes |
, |
T16504 |
416-420 |
NN |
denotes |
AQP2 |
T16505 |
421-426 |
NN |
denotes |
F204V |
T16506 |
420-421 |
HYPH |
denotes |
- |
T16507 |
426-428 |
, |
denotes |
, |
T16508 |
428-430 |
CC |
denotes |
or |
T16509 |
431-433 |
DT |
denotes |
no |
T16510 |
434-440 |
NN |
denotes |
insert |
T16511 |
440-441 |
-RRB- |
denotes |
) |
T16512 |
442-445 |
CC |
denotes |
and |
T16513 |
446-454 |
VBN |
denotes |
selected |
T16514 |
455-459 |
IN |
denotes |
with |
T16515 |
460-463 |
CD |
denotes |
900 |
T16516 |
464-466 |
NN |
denotes |
μg |
T16517 |
470-474 |
NN |
denotes |
G418 |
T16518 |
466-467 |
SYM |
denotes |
/ |
T16519 |
467-469 |
NN |
denotes |
ml |
T16520 |
475-476 |
-LRB- |
denotes |
( |
T16521 |
482-489 |
NNP |
denotes |
Aldrich |
T16522 |
476-481 |
NNP |
denotes |
Sigma |
T16523 |
481-482 |
HYPH |
denotes |
- |
T16524 |
489-490 |
-RRB- |
denotes |
) |
T16525 |
490-491 |
. |
denotes |
. |
T16526 |
491-537 |
sentence |
denotes |
Individual colonies were expanded 14 d later. |
T16527 |
492-502 |
JJ |
denotes |
Individual |
T16528 |
503-511 |
NNS |
denotes |
colonies |
T16529 |
517-525 |
VBN |
denotes |
expanded |
T16530 |
512-516 |
VBD |
denotes |
were |
T16531 |
526-528 |
CD |
denotes |
14 |
T16532 |
529-530 |
NN |
denotes |
d |
T16533 |
531-536 |
RB |
denotes |
later |
T16534 |
536-537 |
. |
denotes |
. |
T16535 |
537-627 |
sentence |
denotes |
For the duration of these experiments, the antibiotic was continually added to the media. |
T16536 |
538-541 |
IN |
denotes |
For |
T16537 |
608-613 |
VBN |
denotes |
added |
T16538 |
542-545 |
DT |
denotes |
the |
T16539 |
546-554 |
NN |
denotes |
duration |
T16540 |
555-557 |
IN |
denotes |
of |
T16541 |
558-563 |
DT |
denotes |
these |
T16542 |
564-575 |
NNS |
denotes |
experiments |
T16543 |
575-577 |
, |
denotes |
, |
T16544 |
577-580 |
DT |
denotes |
the |
T16545 |
581-591 |
NN |
denotes |
antibiotic |
T16546 |
592-595 |
VBD |
denotes |
was |
T16547 |
596-607 |
RB |
denotes |
continually |
T16548 |
614-616 |
IN |
denotes |
to |
T16549 |
617-620 |
DT |
denotes |
the |
T16550 |
621-626 |
NNS |
denotes |
media |
T16551 |
626-627 |
. |
denotes |
. |
T16552 |
627-738 |
sentence |
denotes |
Transient GFP transfections were carried out in subconfluent stable cells lines also using Lipofectamine 2000. |
T16553 |
628-637 |
JJ |
denotes |
Transient |
T16554 |
642-655 |
NNS |
denotes |
transfections |
T16555 |
638-641 |
NN |
denotes |
GFP |
T16556 |
661-668 |
VBN |
denotes |
carried |
T16557 |
656-660 |
VBD |
denotes |
were |
T16558 |
669-672 |
RP |
denotes |
out |
T16559 |
673-675 |
IN |
denotes |
in |
T16560 |
676-688 |
JJ |
denotes |
subconfluent |
T16561 |
702-707 |
NNS |
denotes |
lines |
T16562 |
689-695 |
JJ |
denotes |
stable |
T16563 |
696-701 |
NNS |
denotes |
cells |
T16564 |
708-712 |
RB |
denotes |
also |
T16565 |
713-718 |
VBG |
denotes |
using |
T16566 |
719-732 |
NN |
denotes |
Lipofectamine |
T16567 |
733-737 |
CD |
denotes |
2000 |
T16568 |
737-738 |
. |
denotes |
. |
R4708 |
T16409 |
T16410 |
compound |
MDCK,cells |
R4709 |
T16410 |
T16411 |
nsubjpass |
cells,cultured |
R4710 |
T16412 |
T16413 |
punct |
(,ATCC |
R4711 |
T16413 |
T16410 |
parataxis |
ATCC,cells |
R4712 |
T16414 |
T16413 |
dep |
CCL,ATCC |
R4713 |
T16415 |
T16414 |
punct |
-,CCL |
R4714 |
T16416 |
T16414 |
nummod |
34,CCL |
R4715 |
T16417 |
T16413 |
punct |
;,ATCC |
R4716 |
T16418 |
T16413 |
punct |
", ",ATCC |
R4717 |
T16419 |
T16413 |
npadvmod |
Manassas,ATCC |
R4718 |
T16420 |
T16413 |
punct |
", ",ATCC |
R4719 |
T16421 |
T16413 |
npadvmod |
Virginia,ATCC |
R4720 |
T16422 |
T16413 |
punct |
", ",ATCC |
R4721 |
T16423 |
T16424 |
compound |
United,States |
R4722 |
T16424 |
T16413 |
npadvmod |
States,ATCC |
R4723 |
T16425 |
T16413 |
punct |
),ATCC |
R4724 |
T16426 |
T16411 |
auxpass |
were,cultured |
R4725 |
T16427 |
T16411 |
prep |
in,cultured |
R4726 |
T16428 |
T16427 |
pobj |
DMEM,in |
R4727 |
T16429 |
T16430 |
punct |
(,Aldrich |
R4728 |
T16430 |
T16428 |
parataxis |
Aldrich,DMEM |
R4729 |
T16431 |
T16430 |
compound |
Sigma,Aldrich |
R4730 |
T16432 |
T16430 |
punct |
-,Aldrich |
R4731 |
T16433 |
T16430 |
punct |
", ",Aldrich |
R4732 |
T16434 |
T16435 |
compound |
St.,Louis |
R4733 |
T16435 |
T16430 |
npadvmod |
Louis,Aldrich |
R4734 |
T16436 |
T16430 |
punct |
", ",Aldrich |
R4735 |
T16437 |
T16430 |
npadvmod |
Missouri,Aldrich |
R4736 |
T16438 |
T16430 |
punct |
", ",Aldrich |
R4737 |
T16439 |
T16440 |
compound |
United,States |
R4738 |
T16440 |
T16430 |
npadvmod |
States,Aldrich |
R4739 |
T16441 |
T16430 |
punct |
),Aldrich |
R4740 |
T16442 |
T16411 |
dep |
supplemented,cultured |
R4741 |
T16443 |
T16442 |
prep |
with,supplemented |
R4742 |
T16444 |
T16445 |
nummod |
10,% |
R4743 |
T16445 |
T16446 |
compound |
%,FBS |
R4744 |
T16446 |
T16443 |
pobj |
FBS,with |
R4745 |
T16447 |
T16448 |
punct |
(,Aldrich |
R4746 |
T16448 |
T16446 |
parataxis |
Aldrich,FBS |
R4747 |
T16449 |
T16448 |
compound |
Sigma,Aldrich |
R4748 |
T16450 |
T16448 |
punct |
-,Aldrich |
R4749 |
T16451 |
T16448 |
punct |
),Aldrich |
R4750 |
T16452 |
T16446 |
punct |
", ",FBS |
R4751 |
T16453 |
T16454 |
nummod |
100,U |
R4752 |
T16454 |
T16446 |
conj |
U,FBS |
R4753 |
T16455 |
T16456 |
punct |
/,ml |
R4754 |
T16456 |
T16454 |
prep |
ml,U |
R4755 |
T16457 |
T16454 |
prep |
of,U |
R4756 |
T16458 |
T16457 |
pobj |
penicillin,of |
R4757 |
T16459 |
T16454 |
punct |
", ",U |
R4758 |
T16460 |
T16454 |
cc |
and,U |
R4759 |
T16461 |
T16462 |
nummod |
100,μg |
R4760 |
T16462 |
T16454 |
conj |
μg,U |
R4761 |
T16463 |
T16464 |
punct |
/,ml |
R4762 |
T16464 |
T16462 |
prep |
ml,μg |
R4763 |
T16465 |
T16462 |
prep |
of,μg |
R4764 |
T16466 |
T16465 |
pobj |
streptomycin,of |
R4765 |
T16467 |
T16446 |
prep |
at,FBS |
R4766 |
T16468 |
T16469 |
nummod |
37,°C |
R4767 |
T16469 |
T16467 |
pobj |
°C,at |
R4768 |
T16470 |
T16446 |
prep |
in,FBS |
R4769 |
T16471 |
T16472 |
nummod |
5,% |
R4770 |
T16472 |
T16473 |
compound |
%,CO2 |
R4771 |
T16473 |
T16470 |
pobj |
CO2,in |
R4772 |
T16474 |
T16411 |
punct |
.,cultured |
R4773 |
T16476 |
T16477 |
aux |
To,generate |
R4774 |
T16477 |
T16478 |
advcl |
generate,transfected |
R4775 |
T16479 |
T16480 |
amod |
stable,lines |
R4776 |
T16480 |
T16477 |
dobj |
lines,generate |
R4777 |
T16481 |
T16480 |
compound |
MDCK,lines |
R4778 |
T16482 |
T16480 |
compound |
cell,lines |
R4779 |
T16483 |
T16478 |
punct |
", ",transfected |
R4780 |
T16484 |
T16478 |
nsubjpass |
cells,transfected |
R4781 |
T16485 |
T16478 |
auxpass |
were,transfected |
R4782 |
T16486 |
T16478 |
advcl |
using,transfected |
R4783 |
T16487 |
T16486 |
dobj |
Lipofectamine,using |
R4784 |
T16488 |
T16487 |
nummod |
2000,Lipofectamine |
R4785 |
T16489 |
T16490 |
punct |
(,Invitrogen |
R4786 |
T16490 |
T16487 |
parataxis |
Invitrogen,Lipofectamine |
R4787 |
T16491 |
T16490 |
punct |
),Invitrogen |
R4788 |
T16492 |
T16487 |
cc |
and,Lipofectamine |
R4789 |
T16493 |
T16494 |
det |
the,constructs |
R4790 |
T16494 |
T16487 |
conj |
constructs,Lipofectamine |
R4791 |
T16495 |
T16494 |
compound |
pcDNA3.1,constructs |
R4792 |
T16496 |
T16494 |
compound |
expression,constructs |
R4793 |
T16497 |
T16494 |
punct |
(,constructs |
R4794 |
T16498 |
T16494 |
acl |
containing,constructs |
R4795 |
T16499 |
T16500 |
amod |
wild,type |
R4796 |
T16500 |
T16502 |
compound |
type,AQP2 |
R4797 |
T16501 |
T16500 |
punct |
-,type |
R4798 |
T16502 |
T16498 |
dobj |
AQP2,containing |
R4799 |
T16503 |
T16502 |
punct |
", ",AQP2 |
R4800 |
T16504 |
T16505 |
compound |
AQP2,F204V |
R4801 |
T16505 |
T16502 |
conj |
F204V,AQP2 |
R4802 |
T16506 |
T16505 |
punct |
-,F204V |
R4803 |
T16507 |
T16505 |
punct |
", ",F204V |
R4804 |
T16508 |
T16505 |
cc |
or,F204V |
R4805 |
T16509 |
T16510 |
det |
no,insert |
R4806 |
T16510 |
T16505 |
conj |
insert,F204V |
R4807 |
T16511 |
T16494 |
punct |
),constructs |
R4808 |
T16512 |
T16478 |
cc |
and,transfected |
R4809 |
T16513 |
T16478 |
conj |
selected,transfected |
R4810 |
T16514 |
T16513 |
prep |
with,selected |
R4811 |
T16515 |
T16516 |
nummod |
900,μg |
R4812 |
T16516 |
T16517 |
nmod |
μg,G418 |
R4813 |
T16517 |
T16514 |
pobj |
G418,with |
R4814 |
T16518 |
T16519 |
punct |
/,ml |
R4815 |
T16519 |
T16516 |
prep |
ml,μg |
R4816 |
T16520 |
T16521 |
punct |
(,Aldrich |
R4817 |
T16521 |
T16517 |
parataxis |
Aldrich,G418 |
R4818 |
T16522 |
T16521 |
compound |
Sigma,Aldrich |
R4819 |
T16523 |
T16521 |
punct |
-,Aldrich |
R4820 |
T16524 |
T16521 |
punct |
),Aldrich |
R4821 |
T16525 |
T16478 |
punct |
.,transfected |
R4822 |
T16527 |
T16528 |
amod |
Individual,colonies |
R4823 |
T16528 |
T16529 |
nsubjpass |
colonies,expanded |
R4824 |
T16530 |
T16529 |
auxpass |
were,expanded |
R4825 |
T16531 |
T16532 |
nummod |
14,d |
R4826 |
T16532 |
T16533 |
npadvmod |
d,later |
R4827 |
T16533 |
T16529 |
advmod |
later,expanded |
R4828 |
T16534 |
T16529 |
punct |
.,expanded |
R4829 |
T16536 |
T16537 |
prep |
For,added |
R4830 |
T16538 |
T16539 |
det |
the,duration |
R4831 |
T16539 |
T16536 |
pobj |
duration,For |
R4832 |
T16540 |
T16539 |
prep |
of,duration |
R4833 |
T16541 |
T16542 |
det |
these,experiments |
R4834 |
T16542 |
T16540 |
pobj |
experiments,of |
R4835 |
T16543 |
T16537 |
punct |
", ",added |
R4836 |
T16544 |
T16545 |
det |
the,antibiotic |
R4837 |
T16545 |
T16537 |
nsubjpass |
antibiotic,added |
R4838 |
T16546 |
T16537 |
auxpass |
was,added |
R4839 |
T16547 |
T16537 |
advmod |
continually,added |
R4840 |
T16548 |
T16537 |
prep |
to,added |
R4841 |
T16549 |
T16550 |
det |
the,media |
R4842 |
T16550 |
T16548 |
pobj |
media,to |
R4843 |
T16551 |
T16537 |
punct |
.,added |
R4844 |
T16553 |
T16554 |
amod |
Transient,transfections |
R4845 |
T16554 |
T16556 |
nsubjpass |
transfections,carried |
R4846 |
T16555 |
T16554 |
compound |
GFP,transfections |
R4847 |
T16557 |
T16556 |
auxpass |
were,carried |
R4848 |
T16558 |
T16556 |
prt |
out,carried |
R4849 |
T16559 |
T16556 |
prep |
in,carried |
R4850 |
T16560 |
T16561 |
amod |
subconfluent,lines |
R4851 |
T16561 |
T16559 |
pobj |
lines,in |
R4852 |
T16562 |
T16561 |
amod |
stable,lines |
R4853 |
T16563 |
T16561 |
compound |
cells,lines |
R4854 |
T16564 |
T16556 |
advmod |
also,carried |
R4855 |
T16565 |
T16556 |
advcl |
using,carried |
R4856 |
T16566 |
T16565 |
dobj |
Lipofectamine,using |
R4857 |
T16567 |
T16566 |
nummod |
2000,Lipofectamine |
R4858 |
T16568 |
T16556 |
punct |
.,carried |