Figure 3  S-Opsin Dissociated Cell In Situ Hybridization and S-Opsin/Rhodopsin Antibody Staining on rd7 Mutant Retina
(A–C) A dissociated cell in situ hybridization with an S-opsin probe (red) on dissociated rd7 mutant retinal cells stained with DAPI (blue). (C) shows the merged images.
(D–F) The outer nuclear layer of an rd7 mutant retina stained by antibody for S-opsin (red) and rhodopsin (green). The scleral edge of the outer nuclear layer is up. DAPI staining is in blue. (F) shows the merged images. Insets are higher-power images of the outer segments showing non-overlap of S-opsin and rhodopsin staining in the mutant.