DNA was isolated from paraffin blocks by phenol-chloroform extraction [54,55], and from frozen tissues by standard techniques. Primers were designed to amplify FOG2 coding exons plus 50 bp of flanking upstream and downstream sequence. PCR amplification and sequencing were performed by standard methods. Primer sequences used are listed in Table S2. Sequence analysis was done with Sequencher 4.1 (Gene Codes).