> top > docs > PMC:1134658 > spans > 27095-28440 > annotations

PMC:1134658 / 27095-28440 JSONTXT

Annnotations TAB JSON ListView MergeView

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T15608 0-8 NN denotes Analysis
T15609 9-11 IN denotes of
T15610 12-17 NN denotes BMP-6
T15611 18-27 NN denotes messenger
T15612 28-31 NN denotes RNA
T15613 32-33 -LRB- denotes (
T15614 33-37 NN denotes mRNA
T15615 37-38 -RRB- denotes )
T15616 39-49 NN denotes expression
T15617 50-60 JJ denotes Endogenous
T15618 61-71 NN denotes expression
T15619 72-74 IN denotes of
T15620 75-78 DT denotes the
T15621 79-84 NN denotes BMP-6
T15622 85-89 NN denotes gene
T15623 90-93 VB denotes was
T15624 94-102 VB denotes examined
T15625 103-105 IN denotes by
T15626 106-113 JJ denotes reverse
T15627 114-138 NN denotes transcription-polymerase
T15628 139-144 NN denotes chain
T15629 145-153 NN denotes reaction
T15630 155-160 JJ denotes Total
T15631 161-164 NN denotes RNA
T15632 165-168 VB denotes was
T15633 169-177 VB denotes isolated
T15634 178-183 VB denotes using
T15635 184-194 RB denotes Absolutely
T15636 195-199 NNP denotes RNA™
T15637 200-206 NN denotes RT-PCR
T15638 207-215 NNP denotes Miniprep
T15639 216-219 NN denotes Kit
T15640 220-221 -LRB- denotes (
T15641 221-231 NNP denotes Stratagene
T15642 232-238 NNP denotes Europe
T15643 238-239 -COMMA- denotes ,
T15644 240-249 NNP denotes Amsterdam
T15645 249-250 -COMMA- denotes ,
T15646 251-261 NNP denotes Netherland
T15647 261-262 -RRB- denotes )
T15648 263-272 VB denotes according
T15649 273-275 TO denotes to
T15650 276-279 DT denotes the
T15651 280-293 NN denotes manufacturers
T15652 294-306 NN denotes instructions
T15653 308-322 NN denotes Quantification
T15654 323-325 IN denotes of
T15655 326-329 DT denotes the
T15656 330-338 VB denotes isolated
T15657 339-344 JJ denotes total
T15658 345-348 NN denotes RNA
T15659 349-352 VB denotes was
T15660 353-361 VB denotes achieved
T15661 362-364 IN denotes by
T15662 365-370 VB denotes using
T15663 371-389 JJ denotes spectrophotometric
T15664 390-395 NN denotes OD260
T15665 396-408 NN denotes measurements
T15666 410-415 JJ denotes Equal
T15667 416-423 NN denotes amounts
T15668 424-426 IN denotes of
T15669 427-430 NN denotes RNA
T15670 431-435 VB denotes were
T15671 436-440 RB denotes then
T15672 441-448 VB denotes reverse
T15673 449-460 VB denotes transcribed
T15674 461-463 TO denotes to
T15675 464-468 NN denotes cDNA
T15676 469-473 IN denotes with
T15677 474-481 NNP denotes TaqMan®
T15678 482-489 NNP denotes Reverse
T15679 490-503 NN denotes Transcription
T15680 504-512 NN denotes Reagents
T15681 513-514 -LRB- denotes (
T15682 514-521 NNP denotes Applied
T15683 522-532 NNP denotes Biosystems
T15684 532-533 -COMMA- denotes ,
T15685 534-540 NNP denotes Foster
T15686 541-545 NNP denotes City
T15687 545-546 -COMMA- denotes ,
T15688 547-549 NNP denotes CA
T15689 549-550 -COMMA- denotes ,
T15690 551-556 NN denotes U.S.A
T15691 556-557 -RRB- denotes )
T15692 559-561 TO denotes To
T15693 562-569 VB denotes measure
T15694 570-574 NN denotes mRNA
T15695 575-585 NN denotes expression
T15696 586-588 IN denotes of
T15697 589-593 NN denotes BMP6
T15698 593-594 -COMMA- denotes ,
T15699 595-602 NN denotes Id1–Id4
T15700 603-606 CC denotes and
T15701 607-611 NN denotes PGK1
T15702 612-615 NN denotes PCR
T15703 616-620 VB denotes were
T15704 621-628 VB denotes carried
T15705 629-632 RP denotes out
T15706 633-637 IN denotes with
T15707 638-645 NNP denotes TaqMan®
T15708 646-655 JJ denotes universal
T15709 656-662 NN denotes master
T15710 663-666 NN denotes mix
T15711 668-675 NN denotes Primers
T15712 676-679 CC denotes and
T15713 680-686 NN denotes probes
T15714 687-691 VB denotes were
T15715 692-700 VB denotes provided
T15716 701-703 IN denotes by
T15717 704-719 NN denotes Assay-on-Demand
T15718 720-721 -LRB- denotes (
T15719 721-728 NNP denotes Applied
T15720 729-739 NNP denotes Biosystems
T15721 739-740 -RRB- denotes )
T15722 742-745 NN denotes PCR
T15723 746-755 NN denotes reactions
T15724 756-760 VB denotes were
T15725 761-768 VB denotes carried
T15726 769-772 RP denotes out
T15727 773-775 IN denotes in
T15728 776-777 DT denotes a
T15729 778-783 JJ denotes final
T15730 784-790 NN denotes volume
T15731 791-793 IN denotes of
T15732 794-796 CD denotes 25
T15733 797-799 NN denotes μl
T15734 800-801 -LRB- denotes (
T15735 801-806 NN denotes BMP-6
T15736 806-807 -RRB- denotes )
T15737 808-810 CC denotes or
T15738 811-813 CD denotes 20
T15739 814-816 NN denotes μl
T15740 817-818 -LRB- denotes (
T15741 818-821 NN denotes ID1
T15742 821-822 -RRB- denotes )
T15743 824-827 DT denotes The
T15744 828-832 NN denotes cDNA
T15745 833-838 VB denotes added
T15746 839-841 TO denotes to
T15747 842-846 DT denotes each
T15748 847-855 NN denotes reaction
T15749 856-859 VB denotes was
T15750 860-870 JJ denotes equivalent
T15751 871-873 TO denotes to
T15752 874-877 DT denotes the
T15753 878-883 NN denotes input
T15754 884-886 IN denotes of
T15755 887-889 CD denotes 20
T15756 890-892 NN denotes ng
T15757 893-895 IN denotes of
T15758 896-901 JJ denotes total
T15759 902-905 NN denotes RNA
T15760 907-910 DT denotes The
T15761 911-915 NN denotes gene
T15762 916-926 NN denotes expression
T15763 927-930 VB denotes was
T15764 931-941 VB denotes quantified
T15765 942-947 VB denotes using
T15766 948-951 DT denotes the
T15767 952-960 JJ denotes standard
T15768 961-966 NN denotes curve
T15769 967-973 NN denotes method
T15770 974-975 -LRB- denotes (
T15771 975-979 NN denotes BMP6
T15772 979-980 -RRB- denotes )
T15773 980-981 -COMMA- denotes ,
T15774 982-984 CC denotes or
T15775 985-988 DT denotes the
T15776 989-1000 JJ denotes comparative
T15777 1001-1003 NN denotes CT
T15778 1004-1010 NN denotes method
T15779 1011-1012 -LRB- denotes (
T15780 1012-1015 NN denotes Id1
T15781 1015-1016 -RRB- denotes )
T15782 1017-1019 IN denotes as
T15783 1020-1029 VB denotes described
T15784 1030-1032 IN denotes in
T15785 1033-1040 NNP denotes ABI7700
T15786 1041-1045 NNP denotes User
T15787 1046-1054 NNP denotes Bulletin
T15788 1055-1056 CD denotes 2
T15789 1057-1058 -LRB- denotes (
T15790 1058-1065 NNP denotes Applied
T15791 1066-1076 NNP denotes Biosystems
T15792 1076-1077 -RRB- denotes )
T15793 1079-1082 DT denotes The
T15794 1083-1093 NN denotes expression
T15795 1094-1097 VB denotes was
T15796 1098-1102 RB denotes then
T15797 1103-1113 VB denotes normalized
T15798 1114-1116 TO denotes to
T15799 1117-1120 DT denotes the
T15800 1121-1131 NN denotes expression
T15801 1132-1137 NN denotes level
T15802 1138-1140 IN denotes of
T15803 1141-1145 NN denotes PGK1
T15804 1147-1151 NN denotes PGK1
T15805 1152-1155 VB denotes was
T15806 1156-1162 VB denotes chosen
T15807 1162-1163 -COMMA- denotes ,
T15808 1164-1171 IN denotes because
T15809 1172-1174 PRP denotes it
T15810 1175-1178 VB denotes has
T15811 1179-1183 VB denotes been
T15812 1184-1189 VB denotes shown
T15813 1190-1192 TO denotes to
T15814 1193-1197 VB denotes have
T15815 1198-1201 JJ denotes low
T15816 1202-1212 NN denotes expression
T15817 1213-1224 NN denotes variability
T15818 1225-1230 IN denotes among
T15819 1231-1241 NN denotes lymphocyte
T15820 1242-1251 NN denotes specimens
T15821 1252-1253 -LRB- denotes [
T15822 1253-1255 CD denotes 56
T15823 1255-1256 -RRB- denotes ]
T15824 1258-1268 NN denotes Expression
T15825 1269-1275 NN denotes levels
T15826 1276-1278 IN denotes in
T15827 1279-1280 NN denotes B
T15828 1281-1286 NN denotes cells
T15829 1287-1291 VB denotes were
T15830 1292-1296 RB denotes then
T15831 1297-1304 VB denotes related
T15832 1305-1307 TO denotes to
T15833 1308-1311 DT denotes the
T15834 1312-1322 NN denotes expression
T15835 1323-1329 NN denotes levels
T15836 1330-1332 IN denotes in
T15837 1333-1338 NN denotes Ramos
T15838 1339-1344 NN denotes cells
R11992 T15608 T15609 arg1Of Analysis,of
R11993 T15612 T15609 arg2Of RNA,of
R11994 T15612 T15610 arg1Of RNA,BMP-6
R11995 T15612 T15611 arg1Of RNA,messenger
R11996 T15612 T15613 arg1Of RNA,(
R11997 T15614 T15613 arg2Of mRNA,(
R11998 T15615 T15613 arg3Of ),(
R11999 T15616 T15608 arg1Of expression,Analysis
R12000 T15618 T15617 arg1Of expression,Endogenous
R12001 T15618 T15619 arg1Of expression,of
R12002 T15618 T15623 arg1Of expression,was
R12003 T15618 T15624 arg2Of expression,examined
R12004 T15622 T15619 arg2Of gene,of
R12005 T15622 T15620 arg1Of gene,the
R12006 T15622 T15621 arg1Of gene,BMP-6
R12007 T15624 T15623 arg2Of examined,was
R12008 T15629 T15624 arg1Of reaction,examined
R12009 T15629 T15625 arg2Of reaction,by
R12010 T15629 T15626 arg1Of reaction,reverse
R12011 T15629 T15627 arg1Of reaction,transcription-polymerase
R12012 T15629 T15628 arg1Of reaction,chain
R12013 T15631 T15630 arg1Of RNA,Total
R12014 T15631 T15632 arg1Of RNA,was
R12015 T15631 T15633 arg2Of RNA,isolated
R12016 T15633 T15632 arg2Of isolated,was
R12017 T15634 T15633 arg3Of using,isolated
R12018 T15634 T15635 arg1Of using,Absolutely
R12019 T15634 T15648 arg1Of using,according
R12020 T15638 T15636 arg1Of Miniprep,RNA™
R12021 T15638 T15637 arg1Of Miniprep,RT-PCR
R12022 T15639 T15634 arg2Of Kit,using
R12023 T15639 T15638 arg1Of Kit,Miniprep
R12024 T15639 T15640 arg1Of Kit,(
R12025 T15642 T15641 arg1Of Europe,Stratagene
R12026 T15642 T15643 arg1Of Europe,","
R12027 T15643 T15640 arg2Of ",",(
R12028 T15643 T15645 arg1Of ",",","
R12029 T15643 T15646 arg1Of ",",Netherland
R12030 T15644 T15643 arg2Of Amsterdam,","
R12031 T15647 T15640 arg3Of ),(
R12032 T15649 T15648 arg2Of to,according
R12033 T15652 T15649 arg2Of instructions,to
R12034 T15652 T15650 arg1Of instructions,the
R12035 T15652 T15651 arg1Of instructions,manufacturers
R12036 T15653 T15654 arg1Of Quantification,of
R12037 T15653 T15659 arg1Of Quantification,was
R12038 T15653 T15660 arg2Of Quantification,achieved
R12039 T15658 T15654 arg2Of RNA,of
R12040 T15658 T15655 arg1Of RNA,the
R12041 T15658 T15656 arg2Of RNA,isolated
R12042 T15658 T15657 arg1Of RNA,total
R12043 T15660 T15659 arg2Of achieved,was
R12044 T15660 T15661 arg1Of achieved,by
R12045 T15662 T15661 arg2Of using,by
R12046 T15665 T15662 arg2Of measurements,using
R12047 T15665 T15663 arg1Of measurements,spectrophotometric
R12048 T15665 T15664 arg1Of measurements,OD260
R12049 T15667 T15666 arg1Of amounts,Equal
R12050 T15667 T15668 arg1Of amounts,of
R12051 T15667 T15670 arg1Of amounts,were
R12052 T15669 T15668 arg2Of RNA,of
R12053 T15670 T15671 arg1Of were,then
R12054 T15672 T15670 arg2Of reverse,were
R12055 T15672 T15673 modOf reverse,transcribed
R12056 T15673 T15674 arg1Of transcribed,to
R12057 T15675 T15674 arg2Of cDNA,to
R12058 T15675 T15676 arg1Of cDNA,with
R12059 T15678 T15677 arg1Of Reverse,TaqMan®
R12060 T15680 T15676 arg2Of Reagents,with
R12061 T15680 T15678 arg1Of Reagents,Reverse
R12062 T15680 T15679 arg1Of Reagents,Transcription
R12063 T15680 T15681 arg1Of Reagents,(
R12064 T15683 T15682 arg1Of Biosystems,Applied
R12065 T15683 T15684 arg1Of Biosystems,","
R12066 T15684 T15687 arg1Of ",",","
R12067 T15686 T15684 arg2Of City,","
R12068 T15686 T15685 arg1Of City,Foster
R12069 T15687 T15681 arg2Of ",",(
R12070 T15687 T15689 arg1Of ",",","
R12071 T15688 T15687 arg2Of CA,","
R12072 T15690 T15689 arg2Of U.S.A,","
R12073 T15691 T15681 arg3Of ),(
R12074 T15693 T15692 arg1Of measure,To
R12075 T15695 T15693 arg2Of expression,measure
R12076 T15695 T15694 arg1Of expression,mRNA
R12077 T15695 T15696 arg1Of expression,of
R12078 T15697 T15696 arg2Of BMP6,of
R12079 T15699 T15700 arg1Of Id1–Id4,and
R12080 T15701 T15700 arg2Of PGK1,and
R12081 T15702 T15693 arg1Of PCR,measure
R12082 T15702 T15699 arg1Of PCR,Id1–Id4
R12083 T15702 T15701 arg1Of PCR,PGK1
R12084 T15702 T15703 arg1Of PCR,were
R12085 T15702 T15704 arg2Of PCR,carried
R12086 T15704 T15692 modOf carried,To
R12087 T15704 T15698 arg1Of carried,","
R12088 T15704 T15703 arg2Of carried,were
R12089 T15704 T15705 arg1Of carried,out
R12090 T15704 T15706 arg1Of carried,with
R12091 T15710 T15706 arg2Of mix,with
R12092 T15710 T15707 arg1Of mix,TaqMan®
R12093 T15710 T15708 arg1Of mix,universal
R12094 T15710 T15709 arg1Of mix,master
R12095 T15711 T15712 arg1Of Primers,and
R12096 T15712 T15714 arg1Of and,were
R12097 T15712 T15715 arg2Of and,provided
R12098 T15713 T15712 arg2Of probes,and
R12099 T15715 T15714 arg2Of provided,were
R12100 T15717 T15715 arg1Of Assay-on-Demand,provided
R12101 T15717 T15716 arg2Of Assay-on-Demand,by
R12102 T15717 T15718 arg1Of Assay-on-Demand,(
R12103 T15720 T15718 arg2Of Biosystems,(
R12104 T15720 T15719 arg1Of Biosystems,Applied
R12105 T15721 T15718 arg3Of ),(
R12106 T15723 T15722 arg1Of reactions,PCR
R12107 T15723 T15724 arg1Of reactions,were
R12108 T15723 T15725 arg2Of reactions,carried
R12109 T15725 T15724 arg2Of carried,were
R12110 T15725 T15726 arg1Of carried,out
R12111 T15725 T15727 arg1Of carried,in
R12112 T15730 T15727 arg2Of volume,in
R12113 T15730 T15728 arg1Of volume,a
R12114 T15730 T15729 arg1Of volume,final
R12115 T15730 T15731 arg1Of volume,of
R12116 T15733 T15732 arg1Of μl,25
R12117 T15733 T15734 arg1Of μl,(
R12118 T15733 T15737 arg1Of μl,or
R12119 T15735 T15734 arg2Of BMP-6,(
R12120 T15736 T15734 arg3Of ),(
R12121 T15737 T15731 arg2Of or,of
R12122 T15739 T15737 arg2Of μl,or
R12123 T15739 T15738 arg1Of μl,20
R12124 T15739 T15740 arg1Of μl,(
R12125 T15741 T15740 arg2Of ID1,(
R12126 T15742 T15740 arg3Of ),(
R12127 T15744 T15743 arg1Of cDNA,The
R12128 T15744 T15745 arg2Of cDNA,added
R12129 T15744 T15749 arg1Of cDNA,was
R12130 T15744 T15750 arg1Of cDNA,equivalent
R12131 T15745 T15746 arg1Of added,to
R12132 T15748 T15746 arg2Of reaction,to
R12133 T15748 T15747 arg1Of reaction,each
R12134 T15750 T15749 arg2Of equivalent,was
R12135 T15750 T15751 arg1Of equivalent,to
R12136 T15753 T15751 arg2Of input,to
R12137 T15753 T15752 arg1Of input,the
R12138 T15753 T15754 arg1Of input,of
R12139 T15756 T15754 arg2Of ng,of
R12140 T15756 T15755 arg1Of ng,20
R12141 T15756 T15757 arg1Of ng,of
R12142 T15759 T15757 arg2Of RNA,of
R12143 T15759 T15758 arg1Of RNA,total
R12144 T15762 T15760 arg1Of expression,The
R12145 T15762 T15761 arg1Of expression,gene
R12146 T15762 T15763 arg1Of expression,was
R12147 T15762 T15764 arg2Of expression,quantified
R12148 T15762 T15765 arg1Of expression,using
R12149 T15764 T15763 arg2Of quantified,was
R12150 T15765 T15764 arg3Of using,quantified
R12151 T15765 T15782 arg1Of using,as
R12152 T15769 T15766 arg1Of method,the
R12153 T15769 T15767 arg1Of method,standard
R12154 T15769 T15768 arg1Of method,curve
R12155 T15769 T15770 arg1Of method,(
R12156 T15769 T15774 arg1Of method,or
R12157 T15771 T15770 arg2Of BMP6,(
R12158 T15772 T15770 arg3Of ),(
R12159 T15774 T15765 arg2Of or,using
R12160 T15774 T15773 arg1Of or,","
R12161 T15778 T15774 arg2Of method,or
R12162 T15778 T15775 arg1Of method,the
R12163 T15778 T15776 arg1Of method,comparative
R12164 T15778 T15777 arg1Of method,CT
R12165 T15778 T15779 arg1Of method,(
R12166 T15780 T15779 arg2Of Id1,(
R12167 T15781 T15779 arg3Of ),(
R12168 T15783 T15782 arg2Of described,as
R12169 T15783 T15784 arg1Of described,in
R12170 T15787 T15784 arg2Of Bulletin,in
R12171 T15787 T15785 arg1Of Bulletin,ABI7700
R12172 T15787 T15786 arg1Of Bulletin,User
R12173 T15787 T15788 arg1Of Bulletin,2
R12174 T15787 T15789 arg1Of Bulletin,(
R12175 T15791 T15789 arg2Of Biosystems,(
R12176 T15791 T15790 arg1Of Biosystems,Applied
R12177 T15792 T15789 arg3Of ),(
R12178 T15794 T15793 arg1Of expression,The
R12179 T15794 T15795 arg1Of expression,was
R12180 T15794 T15797 arg2Of expression,normalized
R12181 T15797 T15795 arg2Of normalized,was
R12182 T15797 T15796 arg1Of normalized,then
R12183 T15797 T15798 arg1Of normalized,to
R12184 T15801 T15798 arg2Of level,to
R12185 T15801 T15799 arg1Of level,the
R12186 T15801 T15800 arg1Of level,expression
R12187 T15801 T15802 arg1Of level,of
R12188 T15803 T15802 arg2Of PGK1,of
R12189 T15804 T15805 arg1Of PGK1,was
R12190 T15804 T15806 arg2Of PGK1,chosen
R12191 T15806 T15805 arg2Of chosen,was
R12192 T15806 T15807 arg1Of chosen,","
R12193 T15806 T15808 arg1Of chosen,because
R12194 T15809 T15810 arg1Of it,has
R12195 T15809 T15811 arg1Of it,been
R12196 T15809 T15812 arg2Of it,shown
R12197 T15809 T15814 arg1Of it,have
R12198 T15812 T15808 arg2Of shown,because
R12199 T15812 T15810 arg2Of shown,has
R12200 T15812 T15811 arg2Of shown,been
R12201 T15812 T15821 arg1Of shown,[
R12202 T15814 T15812 arg3Of have,shown
R12203 T15814 T15813 arg1Of have,to
R12204 T15817 T15814 arg2Of variability,have
R12205 T15817 T15815 arg1Of variability,low
R12206 T15817 T15816 arg1Of variability,expression
R12207 T15817 T15818 arg1Of variability,among
R12208 T15820 T15818 arg2Of specimens,among
R12209 T15820 T15819 arg1Of specimens,lymphocyte
R12210 T15822 T15821 arg2Of 56,[
R12211 T15823 T15821 arg3Of ],[
R12212 T15825 T15824 arg1Of levels,Expression
R12213 T15825 T15826 arg1Of levels,in
R12214 T15825 T15829 arg1Of levels,were
R12215 T15825 T15831 arg2Of levels,related
R12216 T15828 T15826 arg2Of cells,in
R12217 T15828 T15827 arg1Of cells,B
R12218 T15831 T15829 arg2Of related,were
R12219 T15831 T15830 arg1Of related,then
R12220 T15831 T15832 arg1Of related,to
R12221 T15835 T15832 arg2Of levels,to
R12222 T15835 T15833 arg1Of levels,the
R12223 T15835 T15834 arg1Of levels,expression
R12224 T15835 T15836 arg1Of levels,in
R12225 T15838 T15836 arg2Of cells,in
R12226 T15838 T15837 arg1Of cells,Ramos

bionlp-st-ge-2016-coref

Id Subject Object Predicate Lexical cue
T15839 1172-1174 Anaphor denotes it
T15840 1147-1151 Antecedent denotes PGK1
R12227 T15839 T15840 boundBy it,PGK1

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T15878 0-8 NN denotes Analysis
T15879 9-11 IN denotes of
T15880 12-17 NNP denotes BMP-6
T15881 18-27 NN denotes messenger
T15882 28-31 NNP denotes RNA
T15883 32-33 -LRB- denotes (
T15884 33-37 NNP denotes mRNA
T15885 37-38 -RRB- denotes )
T15886 39-49 NN denotes expression
T15887 50-60 JJ denotes Endogenous
T15888 61-71 NN denotes expression
T15889 72-74 IN denotes of
T15890 75-78 DT denotes the
T15891 79-84 NNP denotes BMP-6
T15892 85-89 NN denotes gene
T15893 90-93 VBD denotes was
T15894 94-102 VBN denotes examined
T15895 103-105 IN denotes by
T15896 106-113 JJ denotes reverse
T15897 114-138 NN denotes transcription-polymerase
T15898 139-144 NN denotes chain
T15899 145-153 NN denotes reaction
T15900 153-154 . denotes .
T15901 155-160 JJ denotes Total
T15902 161-164 NNP denotes RNA
T15903 165-168 VBD denotes was
T15904 169-177 VBN denotes isolated
T15905 178-183 VBG denotes using
T15906 184-194 RB denotes Absolutely
T15907 195-198 NNP denotes RNA
T15908 198-199 CD denotes ™
T15909 200-206 NNP denotes RT-PCR
T15910 207-215 NNP denotes Miniprep
T15911 216-219 NNP denotes Kit
T15912 220-221 -LRB- denotes (
T15913 221-231 NNP denotes Stratagene
T15914 232-238 NNP denotes Europe
T15915 238-239 , denotes ,
T15916 240-249 NNP denotes Amsterdam
T15917 249-250 , denotes ,
T15918 251-261 NNP denotes Netherland
T15919 261-262 -RRB- denotes )
T15920 263-272 VBG denotes according
T15921 273-275 TO denotes to
T15922 276-279 DT denotes the
T15923 280-293 NNS denotes manufacturers
T15924 294-306 NNS denotes instructions
T15925 306-307 . denotes .
T15926 308-322 NN denotes Quantification
T15927 323-325 IN denotes of
T15928 326-329 DT denotes the
T15929 330-338 JJ denotes isolated
T15930 339-344 JJ denotes total
T15931 345-348 NNP denotes RNA
T15932 349-352 VBD denotes was
T15933 353-361 VBN denotes achieved
T15934 362-364 IN denotes by
T15935 365-370 VBG denotes using
T15936 371-389 JJ denotes spectrophotometric
T15937 390-395 NNP denotes OD260
T15938 396-408 NNS denotes measurements
T15939 408-409 . denotes .
T15940 410-415 NNP denotes Equal
T15941 416-423 NNS denotes amounts
T15942 424-426 IN denotes of
T15943 427-430 NNP denotes RNA
T15944 431-435 VBD denotes were
T15945 436-440 RB denotes then
T15946 441-448 VB denotes reverse
T15947 449-460 VBN denotes transcribed
T15948 461-463 TO denotes to
T15949 464-468 VB denotes cDNA
T15950 469-473 IN denotes with
T15951 474-480 NNP denotes TaqMan
T15952 480-481 NNP denotes ®
T15953 482-489 VB denotes Reverse
T15954 490-503 NNP denotes Transcription
T15955 504-512 NNP denotes Reagents
T15956 513-514 -LRB- denotes (
T15957 514-521 NNP denotes Applied
T15958 522-532 NNPS denotes Biosystems
T15959 532-533 , denotes ,
T15960 534-540 NNP denotes Foster
T15961 541-545 NNP denotes City
T15962 545-546 , denotes ,
T15963 547-549 NNP denotes CA
T15964 549-550 , denotes ,
T15965 551-556 NNP denotes U.S.A
T15966 556-557 -RRB- denotes )
T15967 557-558 . denotes .
T15968 559-561 TO denotes To
T15969 562-569 VB denotes measure
T15970 570-574 NNP denotes mRNA
T15971 575-585 NN denotes expression
T15972 586-588 IN denotes of
T15973 589-593 NNP denotes BMP6
T15974 593-594 , denotes ,
T15975 595-598 NNP denotes Id1
T15976 599-602 NNP denotes Id4
T15977 603-606 CC denotes and
T15978 607-611 NNP denotes PGK1
T15979 612-615 NNP denotes PCR
T15980 616-620 VBD denotes were
T15981 621-628 VBN denotes carried
T15982 629-632 RP denotes out
T15983 633-637 IN denotes with
T15984 638-644 NNP denotes TaqMan
T15985 644-645 VBD denotes ®
T15986 646-655 JJ denotes universal
T15987 656-662 NN denotes master
T15988 663-666 NN denotes mix
T15989 666-667 . denotes .
T15990 668-675 NNS denotes Primers
T15991 676-679 CC denotes and
T15992 680-686 NNS denotes probes
T15993 687-691 VBD denotes were
T15994 692-700 VBN denotes provided
T15995 701-703 IN denotes by
T15996 704-719 NNP denotes Assay-on-Demand
T15997 720-721 -LRB- denotes (
T15998 721-728 NNP denotes Applied
T15999 729-739 NNPS denotes Biosystems
T16000 739-740 -RRB- denotes )
T16001 740-741 . denotes .
T16002 742-745 NNP denotes PCR
T16003 746-755 NNS denotes reactions
T16004 756-760 VBD denotes were
T16005 761-768 VBN denotes carried
T16006 769-772 RP denotes out
T16007 773-775 IN denotes in
T16008 776-777 DT denotes a
T16009 778-783 JJ denotes final
T16010 784-790 NN denotes volume
T16011 791-793 IN denotes of
T16012 794-796 CD denotes 25
T16013 797-799 NN denotes μl
T16014 800-801 -LRB- denotes (
T16015 801-806 NNP denotes BMP-6
T16016 806-807 -RRB- denotes )
T16017 808-810 CC denotes or
T16018 811-813 CD denotes 20
T16019 814-816 NN denotes μl
T16020 817-818 -LRB- denotes (
T16021 818-821 CD denotes ID1
T16022 821-822 -RRB- denotes )
T16023 822-823 . denotes .
T16024 824-827 DT denotes The
T16025 828-832 NNP denotes cDNA
T16026 833-838 VBD denotes added
T16027 839-841 TO denotes to
T16028 842-846 DT denotes each
T16029 847-855 NN denotes reaction
T16030 856-859 VBD denotes was
T16031 860-870 JJ denotes equivalent
T16032 871-873 TO denotes to
T16033 874-877 DT denotes the
T16034 878-883 NN denotes input
T16035 884-886 IN denotes of
T16036 887-889 CD denotes 20
T16037 890-892 NN denotes ng
T16038 893-895 IN denotes of
T16039 896-901 JJ denotes total
T16040 902-905 NNP denotes RNA
T16041 905-906 . denotes .
T16042 907-910 DT denotes The
T16043 911-915 NN denotes gene
T16044 916-926 NN denotes expression
T16045 927-930 VBD denotes was
T16046 931-941 VBN denotes quantified
T16047 942-947 VBG denotes using
T16048 948-951 DT denotes the
T16049 952-960 JJ denotes standard
T16050 961-966 NN denotes curve
T16051 967-973 NN denotes method
T16052 974-975 -LRB- denotes (
T16053 975-979 NNP denotes BMP6
T16054 979-980 -RRB- denotes )
T16055 980-981 , denotes ,
T16056 982-984 CC denotes or
T16057 985-988 DT denotes the
T16058 989-1000 JJ denotes comparative
T16059 1001-1003 NNP denotes CT
T16060 1004-1010 NN denotes method
T16061 1011-1012 -LRB- denotes (
T16062 1012-1015 NNP denotes Id1
T16063 1015-1016 -RRB- denotes )
T16064 1017-1019 IN denotes as
T16065 1020-1029 VBN denotes described
T16066 1030-1032 IN denotes in
T16067 1033-1040 NNP denotes ABI7700
T16068 1041-1045 NNP denotes User
T16069 1046-1054 NNP denotes Bulletin
T16070 1055-1056 CD denotes 2
T16071 1057-1058 -LRB- denotes (
T16072 1058-1065 NNP denotes Applied
T16073 1066-1076 NNPS denotes Biosystems
T16074 1076-1077 -RRB- denotes )
T16075 1077-1078 . denotes .
T16076 1079-1082 DT denotes The
T16077 1083-1093 NN denotes expression
T16078 1094-1097 VBD denotes was
T16079 1098-1102 RB denotes then
T16080 1103-1113 VBN denotes normalized
T16081 1114-1116 TO denotes to
T16082 1117-1120 DT denotes the
T16083 1121-1131 NN denotes expression
T16084 1132-1137 NN denotes level
T16085 1138-1140 IN denotes of
T16086 1141-1145 NNP denotes PGK1
T16087 1145-1146 . denotes .
T16088 1147-1151 CD denotes PGK1
T16089 1152-1155 VBD denotes was
T16090 1156-1162 VBN denotes chosen
T16091 1162-1163 , denotes ,
T16092 1164-1171 IN denotes because
T16093 1172-1174 PRP denotes it
T16094 1175-1178 VBZ denotes has
T16095 1179-1183 VBN denotes been
T16096 1184-1189 VBN denotes shown
T16097 1190-1192 TO denotes to
T16098 1193-1197 VB denotes have
T16099 1198-1201 JJ denotes low
T16100 1202-1212 NN denotes expression
T16101 1213-1224 NN denotes variability
T16102 1225-1230 IN denotes among
T16103 1231-1241 JJ denotes lymphocyte
T16104 1242-1251 NNS denotes specimens
T16105 1252-1253 NNP denotes [
T16106 1253-1255 CD denotes 56
T16107 1255-1256 NNP denotes ]
T16108 1256-1257 . denotes .
T16109 1258-1268 NN denotes Expression
T16110 1269-1275 NNS denotes levels
T16111 1276-1278 IN denotes in
T16112 1279-1280 NNP denotes B
T16113 1281-1286 NNS denotes cells
T16114 1287-1291 VBD denotes were
T16115 1292-1296 RB denotes then
T16116 1297-1304 VBN denotes related
T16117 1305-1307 TO denotes to
T16118 1308-1311 DT denotes the
T16119 1312-1322 NN denotes expression
T16120 1323-1329 NNS denotes levels
T16121 1330-1332 IN denotes in
T16122 1333-1338 NNP denotes Ramos
T16123 1339-1344 NNS denotes cells
T16124 1344-1345 . denotes .
R12228 T15878 T15888 nmod Analysis,expression
R12229 T15879 T15878 prep of,Analysis
R12230 T15880 T15882 amod BMP-6,RNA
R12231 T15881 T15882 compound messenger,RNA
R12232 T15882 T15879 pobj RNA,of
R12233 T15883 T15882 punct (,RNA
R12234 T15884 T15882 appos mRNA,RNA
R12235 T15885 T15882 punct ),RNA
R12236 T15886 T15888 nmod expression,expression
R12237 T15887 T15888 amod Endogenous,expression
R12238 T15888 T15894 nsubjpass expression,examined
R12239 T15889 T15888 prep of,expression
R12240 T15890 T15892 det the,gene
R12241 T15891 T15892 compound BMP-6,gene
R12242 T15892 T15889 pobj gene,of
R12243 T15893 T15894 auxpass was,examined
R12244 T15894 T15894 ROOT examined,examined
R12245 T15895 T15894 agent by,examined
R12246 T15896 T15899 amod reverse,reaction
R12247 T15897 T15899 compound transcription-polymerase,reaction
R12248 T15898 T15899 compound chain,reaction
R12249 T15899 T15895 pobj reaction,by
R12250 T15900 T15894 punct .,examined
R12251 T15901 T15902 amod Total,RNA
R12252 T15902 T15904 nsubjpass RNA,isolated
R12253 T15903 T15904 auxpass was,isolated
R12254 T15904 T15904 ROOT isolated,isolated
R12255 T15905 T15904 advcl using,isolated
R12256 T15906 T15907 advmod Absolutely,RNA
R12257 T15907 T15920 nmod RNA,according
R12258 T15908 T15911 nummod ™,Kit
R12259 T15909 T15911 compound RT-PCR,Kit
R12260 T15910 T15911 compound Miniprep,Kit
R12261 T15911 T15918 npadvmod Kit,Netherland
R12262 T15912 T15911 punct (,Kit
R12263 T15913 T15914 compound Stratagene,Europe
R12264 T15914 T15911 appos Europe,Kit
R12265 T15915 T15914 punct ",",Europe
R12266 T15916 T15914 conj Amsterdam,Europe
R12267 T15917 T15916 punct ",",Amsterdam
R12268 T15918 T15907 advmod Netherland,RNA
R12269 T15919 T15907 punct ),RNA
R12270 T15920 T15905 dobj according,using
R12271 T15921 T15920 prep to,according
R12272 T15922 T15924 det the,instructions
R12273 T15923 T15924 compound manufacturers,instructions
R12274 T15924 T15921 pobj instructions,to
R12275 T15925 T15904 punct .,isolated
R12276 T15926 T15933 nsubjpass Quantification,achieved
R12277 T15927 T15926 prep of,Quantification
R12278 T15928 T15931 det the,RNA
R12279 T15929 T15931 amod isolated,RNA
R12280 T15930 T15931 amod total,RNA
R12281 T15931 T15927 pobj RNA,of
R12282 T15932 T15933 auxpass was,achieved
R12283 T15933 T15933 ROOT achieved,achieved
R12284 T15934 T15933 prep by,achieved
R12285 T15935 T15934 pcomp using,by
R12286 T15936 T15937 compound spectrophotometric,OD260
R12287 T15937 T15938 compound OD260,measurements
R12288 T15938 T15935 dobj measurements,using
R12289 T15939 T15933 punct .,achieved
R12290 T15940 T15941 compound Equal,amounts
R12291 T15941 T15947 nsubjpass amounts,transcribed
R12292 T15942 T15941 prep of,amounts
R12293 T15943 T15942 pobj RNA,of
R12294 T15944 T15947 auxpass were,transcribed
R12295 T15945 T15946 advmod then,reverse
R12297 T15947 T15947 ROOT transcribed,transcribed
R12298 T15948 T15949 aux to,cDNA
R12299 T15949 T15947 advcl cDNA,transcribed
R12300 T15950 T15949 prep with,cDNA
R12301 T15951 T15952 compound TaqMan,®
R12302 T15952 T15950 pobj ®,with
R12303 T15953 T15953 ROOT Reverse,Reverse
R12304 T15954 T15955 compound Transcription,Reagents
R12305 T15955 T15953 dobj Reagents,Reverse
R12306 T15956 T15958 punct (,Biosystems
R12307 T15957 T15958 compound Applied,Biosystems
R12308 T15958 T15955 appos Biosystems,Reagents
R12309 T15959 T15958 punct ",",Biosystems
R12310 T15960 T15961 compound Foster,City
R12311 T15961 T15958 npadvmod City,Biosystems
R12312 T15962 T15961 punct ",",City
R12313 T15963 T15961 conj CA,City
R12314 T15964 T15963 punct ",",CA
R12315 T15965 T15963 conj U.S.A,CA
R12316 T15966 T15958 punct ),Biosystems
R12317 T15967 T15953 punct .,Reverse
R12318 T15968 T15969 aux To,measure
R12319 T15969 T15981 advcl measure,carried
R12320 T15970 T15971 compound mRNA,expression
R12321 T15971 T15981 nsubjpass expression,carried
R12322 T15972 T15971 prep of,expression
R12323 T15973 T15972 pobj BMP6,of
R12324 T15974 T15973 punct ",",BMP6
R12325 T15975 T15973 conj Id1,BMP6
R12326 T15976 T15971 appos Id4,expression
R12327 T15977 T15976 cc and,Id4
R12328 T15978 T15979 compound PGK1,PCR
R12329 T15979 T15976 conj PCR,Id4
R12330 T15980 T15981 auxpass were,carried
R12331 T15981 T15981 ROOT carried,carried
R12332 T15982 T15981 prt out,carried
R12333 T15983 T15981 prep with,carried
R12334 T15984 T15985 compound TaqMan,®
R12335 T15985 T15981 advcl ®,carried
R12336 T15986 T15988 amod universal,mix
R12337 T15987 T15988 compound master,mix
R12338 T15988 T15985 dobj mix,®
R12339 T15989 T15985 punct .,®
R12340 T15990 T15994 nsubjpass Primers,provided
R12341 T15991 T15990 cc and,Primers
R12342 T15992 T15990 conj probes,Primers
R12343 T15993 T15994 auxpass were,provided
R12344 T15994 T15994 ROOT provided,provided
R12345 T15995 T15994 agent by,provided
R12346 T15996 T15995 pobj Assay-on-Demand,by
R12347 T15997 T15999 punct (,Biosystems
R12348 T15998 T15999 compound Applied,Biosystems
R12349 T15999 T15996 parataxis Biosystems,Assay-on-Demand
R12350 T16000 T15999 punct ),Biosystems
R12351 T16001 T15994 punct .,provided
R12352 T16002 T16003 compound PCR,reactions
R12353 T16003 T16005 nsubjpass reactions,carried
R12354 T16004 T16005 auxpass were,carried
R12355 T16005 T16005 ROOT carried,carried
R12356 T16006 T16005 prt out,carried
R12357 T16007 T16005 prep in,carried
R12358 T16008 T16010 det a,volume
R12359 T16009 T16010 amod final,volume
R12360 T16010 T16007 pobj volume,in
R12361 T16011 T16010 prep of,volume
R12362 T16012 T16013 nummod 25,μl
R12363 T16013 T16011 pobj μl,of
R12364 T16014 T16015 punct (,BMP-6
R12365 T16015 T16013 appos BMP-6,μl
R12366 T16016 T16015 punct ),BMP-6
R12367 T16017 T16013 cc or,μl
R12368 T16018 T16019 nummod 20,μl
R12369 T16019 T16013 conj μl,μl
R12370 T16020 T16021 punct (,ID1
R12371 T16021 T16019 appos ID1,μl
R12372 T16022 T16019 punct ),μl
R12373 T16023 T16005 punct .,carried
R12374 T16024 T16025 det The,cDNA
R12375 T16025 T16026 nsubj cDNA,added
R12376 T16026 T16026 ROOT added,added
R12377 T16027 T16026 prep to,added
R12378 T16028 T16029 det each,reaction
R12379 T16029 T16027 pobj reaction,to
R12380 T16030 T16026 ccomp was,added
R12381 T16031 T16030 acomp equivalent,was
R12382 T16032 T16031 prep to,equivalent
R12383 T16033 T16034 det the,input
R12384 T16034 T16032 pobj input,to
R12385 T16035 T16034 prep of,input
R12386 T16036 T16037 nummod 20,ng
R12387 T16037 T16035 pobj ng,of
R12388 T16038 T16037 prep of,ng
R12389 T16039 T16040 amod total,RNA
R12390 T16040 T16038 pobj RNA,of
R12391 T16041 T16026 punct .,added
R12392 T16042 T16044 det The,expression
R12393 T16043 T16044 compound gene,expression
R12394 T16044 T16046 nsubjpass expression,quantified
R12395 T16045 T16046 auxpass was,quantified
R12396 T16046 T16046 ROOT quantified,quantified
R12397 T16047 T16046 advcl using,quantified
R12398 T16048 T16051 det the,method
R12399 T16049 T16051 amod standard,method
R12400 T16050 T16051 compound curve,method
R12401 T16051 T16047 dobj method,using
R12402 T16052 T16053 punct (,BMP6
R12403 T16053 T16051 appos BMP6,method
R12404 T16054 T16053 punct ),BMP6
R12405 T16055 T16051 punct ",",method
R12406 T16056 T16051 cc or,method
R12407 T16057 T16060 det the,method
R12408 T16058 T16060 amod comparative,method
R12409 T16059 T16060 compound CT,method
R12410 T16060 T16051 conj method,method
R12411 T16061 T16062 punct (,Id1
R12412 T16062 T16060 appos Id1,method
R12413 T16063 T16060 punct ),method
R12414 T16064 T16065 mark as,described
R12415 T16065 T16046 advcl described,quantified
R12416 T16066 T16065 prep in,described
R12417 T16067 T16069 compound ABI7700,Bulletin
R12418 T16068 T16069 compound User,Bulletin
R12419 T16069 T16066 pobj Bulletin,in
R12420 T16070 T16069 nummod 2,Bulletin
R12421 T16071 T16073 punct (,Biosystems
R12422 T16072 T16073 compound Applied,Biosystems
R12423 T16073 T16069 appos Biosystems,Bulletin
R12424 T16074 T16073 punct ),Biosystems
R12425 T16075 T16046 punct .,quantified
R12426 T16076 T16077 det The,expression
R12427 T16077 T16078 nsubj expression,was
R12428 T16078 T16078 ROOT was,was
R12429 T16079 T16080 advmod then,normalized
R12430 T16080 T16078 acomp normalized,was
R12431 T16081 T16080 prep to,normalized
R12432 T16082 T16084 det the,level
R12433 T16083 T16084 compound expression,level
R12434 T16084 T16081 pobj level,to
R12435 T16085 T16084 prep of,level
R12436 T16086 T16085 pobj PGK1,of
R12437 T16087 T16078 punct .,was
R12438 T16088 T16090 nsubjpass PGK1,chosen
R12439 T16089 T16090 auxpass was,chosen
R12440 T16090 T16090 ROOT chosen,chosen
R12441 T16091 T16090 punct ",",chosen
R12442 T16092 T16096 mark because,shown
R12443 T16093 T16096 nsubjpass it,shown
R12444 T16094 T16096 aux has,shown
R12445 T16095 T16096 auxpass been,shown
R12446 T16096 T16090 advcl shown,chosen
R12447 T16097 T16098 aux to,have
R12448 T16098 T16096 xcomp have,shown
R12449 T16099 T16101 amod low,variability
R12450 T16100 T16101 compound expression,variability
R12451 T16101 T16098 dobj variability,have
R12452 T16102 T16101 prep among,variability
R12453 T16103 T16104 amod lymphocyte,specimens
R12454 T16104 T16102 pobj specimens,among
R12455 T16105 T16107 nmod [,]
R12456 T16106 T16107 nummod 56,]
R12457 T16107 T16101 appos ],variability
R12458 T16108 T16090 punct .,chosen
R12459 T16109 T16110 compound Expression,levels
R12460 T16110 T16116 nsubjpass levels,related
R12461 T16111 T16110 prep in,levels
R12462 T16112 T16113 compound B,cells
R12463 T16113 T16111 pobj cells,in
R12464 T16114 T16116 auxpass were,related
R12465 T16115 T16116 advmod then,related
R12466 T16116 T16116 ROOT related,related
R12467 T16117 T16116 prep to,related
R12468 T16118 T16120 det the,levels
R12469 T16119 T16120 compound expression,levels
R12470 T16120 T16117 pobj levels,to
R12471 T16121 T16120 prep in,levels
R12472 T16122 T16123 compound Ramos,cells
R12473 T16123 T16121 pobj cells,in
R12474 T16124 T16116 punct .,related
R12296 T15946 T15947 advmod reverse,transcribed

GO-BP

Id Subject Object Predicate Lexical cue
T15601 106-127 http://purl.obolibrary.org/obo/GO_0001171 denotes reverse transcription
T15602 482-503 http://purl.obolibrary.org/obo/GO_0001171 denotes Reverse Transcription
T15603 114-127 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T15604 490-503 http://purl.obolibrary.org/obo/GO_0006351 denotes Transcription
T15605 200-202 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T15606 547-549 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T15607 911-926 http://purl.obolibrary.org/obo/GO_0010467 denotes gene expression

GO-MF

Id Subject Object Predicate Lexical cue
T16125 200-202 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T16126 547-549 http://purl.obolibrary.org/obo/GO_0033968 denotes CA

GO-CC

Id Subject Object Predicate Lexical cue
T16127 1281-1286 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16128 1339-1344 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

sentences

Id Subject Object Predicate Lexical cue
T15588 0-49 Sentence denotes Analysis of BMP-6 messenger RNA (mRNA) expression
T15589 50-154 Sentence denotes Endogenous expression of the BMP-6 gene was examined by reverse transcription-polymerase chain reaction.
T15590 155-307 Sentence denotes Total RNA was isolated using Absolutely RNA™ RT-PCR Miniprep Kit (Stratagene Europe, Amsterdam, Netherland) according to the manufacturers instructions.
T15591 308-409 Sentence denotes Quantification of the isolated total RNA was achieved by using spectrophotometric OD260 measurements.
T15592 410-558 Sentence denotes Equal amounts of RNA were then reverse transcribed to cDNA with TaqMan® Reverse Transcription Reagents (Applied Biosystems, Foster City, CA, U.S.A).
T15593 559-667 Sentence denotes To measure mRNA expression of BMP6, Id1–Id4 and PGK1 PCR were carried out with TaqMan® universal master mix.
T15594 668-741 Sentence denotes Primers and probes were provided by Assay-on-Demand (Applied Biosystems).
T15595 742-823 Sentence denotes PCR reactions were carried out in a final volume of 25 μl (BMP-6) or 20 μl (ID1).
T15596 824-906 Sentence denotes The cDNA added to each reaction was equivalent to the input of 20 ng of total RNA.
T15597 907-1078 Sentence denotes The gene expression was quantified using the standard curve method (BMP6), or the comparative CT method (Id1) as described in ABI7700 User Bulletin 2 (Applied Biosystems).
T15598 1079-1146 Sentence denotes The expression was then normalized to the expression level of PGK1.
T15599 1147-1257 Sentence denotes PGK1 was chosen, because it has been shown to have low expression variability among lymphocyte specimens [56].
T15600 1258-1345 Sentence denotes Expression levels in B cells were then related to the expression levels in Ramos cells.
T202 0-49 Sentence denotes Analysis of BMP-6 messenger RNA (mRNA) expression
T203 50-154 Sentence denotes Endogenous expression of the BMP-6 gene was examined by reverse transcription-polymerase chain reaction.
T204 155-307 Sentence denotes Total RNA was isolated using Absolutely RNA™ RT-PCR Miniprep Kit (Stratagene Europe, Amsterdam, Netherland) according to the manufacturers instructions.
T205 308-409 Sentence denotes Quantification of the isolated total RNA was achieved by using spectrophotometric OD260 measurements.
T206 410-558 Sentence denotes Equal amounts of RNA were then reverse transcribed to cDNA with TaqMan® Reverse Transcription Reagents (Applied Biosystems, Foster City, CA, U.S.A).
T207 559-667 Sentence denotes To measure mRNA expression of BMP6, Id1–Id4 and PGK1 PCR were carried out with TaqMan® universal master mix.
T208 668-741 Sentence denotes Primers and probes were provided by Assay-on-Demand (Applied Biosystems).
T209 742-823 Sentence denotes PCR reactions were carried out in a final volume of 25 μl (BMP-6) or 20 μl (ID1).
T210 824-906 Sentence denotes The cDNA added to each reaction was equivalent to the input of 20 ng of total RNA.
T211 907-1078 Sentence denotes The gene expression was quantified using the standard curve method (BMP6), or the comparative CT method (Id1) as described in ABI7700 User Bulletin 2 (Applied Biosystems).
T212 1079-1146 Sentence denotes The expression was then normalized to the expression level of PGK1.
T213 1147-1257 Sentence denotes PGK1 was chosen, because it has been shown to have low expression variability among lymphocyte specimens [56].
T214 1258-1345 Sentence denotes Expression levels in B cells were then related to the expression levels in Ramos cells.

events-check-again

Id Subject Object Predicate Lexical cue Speculation
T16190 12-17 Protein denotes BMP-6
T16191 39-49 Transcription denotes expression true
T16192 39-49 Transcription denotes expression true
T16193 39-49 Transcription denotes expression true
T16194 39-49 Transcription denotes expression true
T16195 61-71 Gene_expression denotes expression true
T16196 79-84 Protein denotes BMP-6
T16197 570-585 Transcription denotes mRNA expression true
T16198 570-585 Transcription denotes mRNA expression true
T16199 570-585 Transcription denotes mRNA expression true
T16200 570-585 Transcription denotes mRNA expression true
T16201 589-593 Protein denotes BMP6
T16202 595-598 Protein denotes Id1
T16203 599-602 Protein denotes Id4
T16204 607-611 Protein denotes PGK1
T16205 801-806 Protein denotes BMP-6
T16206 916-926 Gene_expression denotes expression true
T16207 916-926 Gene_expression denotes expression true
T16208 975-979 Protein denotes BMP6
T16209 1012-1015 Protein denotes Id1
T16210 1121-1131 Gene_expression denotes expression true
T16211 1141-1145 Protein denotes PGK1
T16212 1147-1151 Protein denotes PGK1
T16213 1193-1212 Gene_expression denotes have low expression
R12507 T16196 T16195 themeOf BMP-6,expression
R12508 T16201 T16193 themeOf BMP6,expression
R12509 T16201 T16200 themeOf BMP6,mRNA expression
R12510 T16202 T16194 themeOf Id1,expression
R12511 T16202 T16198 themeOf Id1,mRNA expression
R12512 T16203 T16199 themeOf Id4,mRNA expression
R12513 T16203 T16192 themeOf Id4,expression
R12514 T16204 T16197 themeOf PGK1,mRNA expression
R12515 T16204 T16191 themeOf PGK1,expression
R12516 T16208 T16206 themeOf BMP6,expression
R12517 T16209 T16207 themeOf Id1,expression
R12518 T16211 T16210 themeOf PGK1,expression
R12519 T16212 T16213 themeOf PGK1,have low expression

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T16129 12-31 Protein denotes BMP-6 messenger RNA
T16130 39-49 Gene_expression denotes expression
T16131 575-585 Transcription denotes expression
T16132 575-585 Transcription denotes expression
T16133 575-585 Transcription denotes expression
T16134 575-585 Transcription denotes expression
T16135 589-593 Protein denotes BMP6
T16136 595-598 Protein denotes Id1
T16137 599-602 Protein denotes Id4
T16138 607-615 Protein denotes PGK1 PCR
T16139 1121-1131 Gene_expression denotes expression
T16140 1141-1145 Protein denotes PGK1
T16141 1147-1151 Protein denotes PGK1
R12477 T16136 T16132 themeOf Id1,expression
R12478 T16137 T16133 themeOf Id4,expression
R12479 T16138 T16134 themeOf PGK1 PCR,expression
R12480 T16140 T16139 themeOf PGK1,expression
R12475 T16129 T16130 themeOf BMP-6 messenger RNA,expression
R12476 T16135 T16131 themeOf BMP6,expression

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue Speculation
T15584 1121-1131 Gene_expression denotes expression true
T15585 1141-1145 Protein denotes PGK1
T15586 1147-1151 Protein denotes PGK1
T15587 1193-1212 Gene_expression denotes have low expression
T15564 12-17 Protein denotes BMP-6
T15565 39-49 Transcription denotes expression true
T15566 39-49 Transcription denotes expression true
T15567 39-49 Transcription denotes expression true
T15568 39-49 Transcription denotes expression true
T15569 61-71 Gene_expression denotes expression true
T15570 79-84 Protein denotes BMP-6
T15571 570-585 Transcription denotes mRNA expression true
T15572 570-585 Transcription denotes mRNA expression true
T15573 570-585 Transcription denotes mRNA expression true
T15574 570-585 Transcription denotes mRNA expression true
T15575 589-593 Protein denotes BMP6
T15576 595-598 Protein denotes Id1
T15577 599-602 Protein denotes Id4
T15578 607-611 Protein denotes PGK1
T15579 801-806 Protein denotes BMP-6
T15580 916-926 Gene_expression denotes expression true
T15581 916-926 Gene_expression denotes expression true
T15582 975-979 Protein denotes BMP6
T15583 1012-1015 Protein denotes Id1
R11979 T15570 T15569 themeOf BMP-6,expression
R11980 T15575 T15567 themeOf BMP6,expression
R11981 T15575 T15574 themeOf BMP6,mRNA expression
R11982 T15576 T15568 themeOf Id1,expression
R11983 T15576 T15572 themeOf Id1,mRNA expression
R11984 T15577 T15573 themeOf Id4,mRNA expression
R11985 T15577 T15566 themeOf Id4,expression
R11986 T15578 T15571 themeOf PGK1,mRNA expression
R11987 T15578 T15565 themeOf PGK1,expression
R11988 T15582 T15580 themeOf BMP6,expression
R11989 T15583 T15581 themeOf Id1,expression
R11990 T15585 T15584 themeOf PGK1,expression
R11991 T15586 T15587 themeOf PGK1,have low expression

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T15841 12-15 P22004 denotes BMP
T15842 12-15 P18075 denotes BMP
T15843 12-15 P12644 denotes BMP
T15844 12-15 P12643 denotes BMP
T15845 12-17 P22004 denotes BMP-6
T15846 79-82 P18075 denotes BMP
T15847 79-82 P12643 denotes BMP
T15848 79-82 P12644 denotes BMP
T15849 79-82 P22004 denotes BMP
T15850 79-84 P22004 denotes BMP-6
T15851 589-593 P22004 denotes BMP6
T15852 595-598 P41134 denotes Id1
T15853 599-602 P47928 denotes Id4
T15854 607-611 P00558 denotes PGK1
T15855 801-804 P22004 denotes BMP
T15856 801-804 P12644 denotes BMP
T15857 801-804 P18075 denotes BMP
T15858 801-804 P12643 denotes BMP
T15859 801-806 P22004 denotes BMP-6
T15860 818-821 P41134 denotes ID1
T15861 975-979 P22004 denotes BMP6
T15862 1012-1015 P41134 denotes Id1
T15863 1141-1145 P00558 denotes PGK1
T15864 1147-1151 P00558 denotes PGK1

test2

Id Subject Object Predicate Lexical cue Speculation
T15549 12-17 Protein denotes BMP-6 true
T15550 61-71 Gene_expression denotes expression true
T15551 79-84 Protein denotes BMP-6 true
T15552 570-585 Transcription denotes mRNA expression true
T15553 589-593 Protein denotes BMP6 true
T15554 595-598 Protein denotes Id1 true
T15555 599-602 Protein denotes Id4 true
T15556 607-611 Protein denotes PGK1 true
T15557 801-806 Protein denotes BMP-6 true
T15558 975-979 Protein denotes BMP6 true
T15559 1012-1015 Protein denotes Id1 true
T15560 1121-1131 Gene_expression denotes expression true
T15561 1141-1145 Protein denotes PGK1
T15562 1147-1151 Protein denotes PGK1
T15563 1258-1268 Gene_expression denotes Expression
R11973 T15551 T15550 themeOf BMP-6,expression
R11974 T15553 T15552 themeOf BMP6,mRNA expression
R11975 T15554 T15552 themeOf Id1,mRNA expression
R11976 T15555 T15552 themeOf Id4,mRNA expression
R11977 T15556 T15552 themeOf PGK1,mRNA expression
R11978 T15561 T15560 themeOf PGK1,expression

tees-test

Id Subject Object Predicate Lexical cue
T3131 12-17 D055418 denotes BMP-6
T3132 12-17 D055418 denotes BMP-6
T3133 12-17 Q04906 denotes BMP-6
T3134 12-17 PR:000000167 denotes BMP-6
T3135 12-17 P20722 denotes BMP-6
T3136 12-17 PR:Q04906 denotes BMP-6
T3137 12-17 P22004 denotes BMP-6
T3138 12-17 PR:P22004 denotes BMP-6
T3139 12-17 PR:P20722 denotes BMP-6
T3140 18-31 D012333 denotes messenger RNA
T3141 18-31 CHEBI:33699 denotes messenger RNA
T3142 18-31 SO:0000234 denotes messenger RNA
T3143 28-31 GO:0005562 denotes RNA
T3144 33-37 D012333 denotes mRNA
T3145 33-37 CHEBI:33699 denotes mRNA
T3146 33-37 SO:0000234 denotes mRNA
T3147 79-84 D055418 denotes BMP-6
T3148 79-84 D055418 denotes BMP-6
T3149 79-84 Q04906 denotes BMP-6
T3150 79-84 PR:000000167 denotes BMP-6
T3151 79-84 P20722 denotes BMP-6
T3152 79-84 PR:Q04906 denotes BMP-6
T3153 79-84 P22004 denotes BMP-6
T3154 79-84 PR:P22004 denotes BMP-6
T3155 79-84 PR:P20722 denotes BMP-6
T3156 85-89 SO:0000704 denotes gene
T3157 106-113 SO:0001031 denotes reverse
T3158 106-127 GO:0001171 denotes reverse transcription
T3159 139-144 SO:0000419 denotes chain
T3160 139-153 MOP:0000635 denotes chain reaction
T3161 161-164 CHEBI:33697 denotes RNA
T3162 161-164 D012313 denotes RNA
T3163 161-164 GO:0005562 denotes RNA
T3164 161-164 SO:0000356 denotes RNA
T3165 195-198 CHEBI:33697 denotes RNA
T3166 195-198 D012313 denotes RNA
T3167 195-198 GO:0005562 denotes RNA
T3168 195-198 SO:0000356 denotes RNA
T3169 203-206 Q41249 denotes PCR
T3170 203-206 O66148 denotes PCR
T3171 203-206 Q59987 denotes PCR
T3172 203-206 Q01289 denotes PCR
T3173 203-206 O80333 denotes PCR
T3174 203-206 P15904 denotes PCR
T3175 203-206 Q9SDT1 denotes PCR
T3176 203-206 Q39617 denotes PCR
T3177 216-219 PR:P05532 denotes Kit
T3178 345-348 CHEBI:33697 denotes RNA
T3179 345-348 D012313 denotes RNA
T3180 345-348 GO:0005562 denotes RNA
T3181 345-348 SO:0000356 denotes RNA
T3182 427-430 CHEBI:33697 denotes RNA
T3183 427-430 D012313 denotes RNA
T3184 427-430 GO:0005562 denotes RNA
T3185 427-430 SO:0000356 denotes RNA
T3186 441-448 SO:0001031 denotes reverse
T3187 464-468 D018076 denotes cDNA
T3188 464-468 SO:0000756 denotes cDNA
T3189 482-489 SO:0001031 denotes Reverse
T3190 482-503 GO:0001171 denotes Reverse Transcription
T3191 504-512 CHEBI:33893 denotes Reagents
T3192 504-512 D007202 denotes Reagents
T3193 570-574 D012333 denotes mRNA
T3194 570-574 CHEBI:33699 denotes mRNA
T3195 570-574 SO:0000234 denotes mRNA
T3196 589-593 D055418 denotes BMP6
T3197 589-593 D055418 denotes BMP6
T3198 589-593 Q04906 denotes BMP6
T3199 589-593 PR:000000167 denotes BMP6
T3200 589-593 P20722 denotes BMP6
T3201 589-593 PR:Q04906 denotes BMP6
T3202 589-593 P22004 denotes BMP6
T3203 589-593 PR:P22004 denotes BMP6
T3204 589-593 PR:P20722 denotes BMP6
T3205 595-598 PR:P41134 denotes Id1
T3206 595-598 PR:P20067 denotes Id1
T3207 595-598 PR:P41135 denotes Id1
T3208 595-598 PR:000000039 denotes Id1
T3209 599-602 PR:P47928 denotes Id4
T3210 599-602 PR:P41139 denotes Id4
T3211 599-602 PR:000000042 denotes Id4
T3212 607-610 GO:0004618 denotes PGK
T3213 607-611 PR:P00558 denotes PGK1
T3214 607-611 PR:P91427 denotes PGK1
T3215 607-611 PR:P09411 denotes PGK1
T3216 607-611 PR:P00560 denotes PGK1
T3217 607-611 PR:O60101 denotes PGK1
T3218 607-611 PR:P46273 denotes PGK1
T3219 607-611 PR:000012607 denotes PGK1
T3220 607-611 PR:Q9LD57 denotes PGK1
T3221 607-611 PR:P16617 denotes PGK1
T3222 612-615 Q41249 denotes PCR
T3223 612-615 O66148 denotes PCR
T3224 612-615 Q59987 denotes PCR
T3225 612-615 Q01289 denotes PCR
T3226 612-615 O80333 denotes PCR
T3227 612-615 P15904 denotes PCR
T3228 612-615 Q9SDT1 denotes PCR
T3229 612-615 Q39617 denotes PCR
T3230 742-745 Q41249 denotes PCR
T3231 742-745 O66148 denotes PCR
T3232 742-745 Q59987 denotes PCR
T3233 742-745 Q01289 denotes PCR
T3234 742-745 O80333 denotes PCR
T3235 742-745 P15904 denotes PCR
T3236 742-745 Q9SDT1 denotes PCR
T3237 742-745 Q39617 denotes PCR
T3238 801-806 Q04906 denotes BMP-6
T3239 801-806 PR:000000167 denotes BMP-6
T3240 801-806 P20722 denotes BMP-6
T3241 801-806 PR:Q04906 denotes BMP-6
T3242 801-806 P22004 denotes BMP-6
T3243 801-806 PR:P22004 denotes BMP-6
T3244 801-806 PR:P20722 denotes BMP-6
T3245 801-806 D055418 denotes BMP-6
T3246 801-806 D055418 denotes BMP-6
T3247 818-821 PR:P41134 denotes ID1
T3248 818-821 PR:P20067 denotes ID1
T3249 818-821 PR:P41135 denotes ID1
T3250 818-821 PR:000000039 denotes ID1
T3251 828-832 SO:0000756 denotes cDNA
T3252 828-832 D018076 denotes cDNA
T3253 902-905 SO:0000356 denotes RNA
T3254 902-905 CHEBI:33697 denotes RNA
T3255 902-905 D012313 denotes RNA
T3256 902-905 GO:0005562 denotes RNA
T3257 911-915 SO:0000704 denotes gene
T3258 911-926 GO:0010467 denotes gene expression
T3259 975-979 D055418 denotes BMP6
T3260 975-979 D055418 denotes BMP6
T3261 975-979 Q04906 denotes BMP6
T3262 975-979 PR:000000167 denotes BMP6
T3263 975-979 P20722 denotes BMP6
T3264 975-979 PR:Q04906 denotes BMP6
T3265 975-979 P22004 denotes BMP6
T3266 975-979 PR:P22004 denotes BMP6
T3267 975-979 PR:P20722 denotes BMP6
T3268 1012-1015 PR:P41134 denotes Id1
T3269 1012-1015 PR:P20067 denotes Id1
T3270 1012-1015 PR:P41135 denotes Id1
T3271 1012-1015 PR:000000039 denotes Id1
T3272 1141-1144 GO:0004618 denotes PGK
T3273 1141-1145 PR:P00558 denotes PGK1
T3274 1141-1145 PR:P91427 denotes PGK1
T3275 1141-1145 PR:P09411 denotes PGK1
T3276 1141-1145 PR:P00560 denotes PGK1
T3277 1141-1145 PR:O60101 denotes PGK1
T3278 1141-1145 PR:P46273 denotes PGK1
T3279 1141-1145 PR:000012607 denotes PGK1
T3280 1141-1145 PR:Q9LD57 denotes PGK1
T3281 1141-1145 PR:P16617 denotes PGK1
T3282 1147-1150 GO:0004618 denotes PGK
T3283 1147-1151 PR:P00558 denotes PGK1
T3284 1147-1151 PR:P91427 denotes PGK1
T3285 1147-1151 PR:P09411 denotes PGK1
T3286 1147-1151 PR:P00560 denotes PGK1
T3287 1147-1151 PR:O60101 denotes PGK1
T3288 1147-1151 PR:P46273 denotes PGK1
T3289 1147-1151 PR:000012607 denotes PGK1
T3290 1147-1151 PR:Q9LD57 denotes PGK1
T3291 1147-1151 PR:P16617 denotes PGK1
T3292 1231-1241 CL:0000542 denotes lymphocyte
T3293 1333-1338 CVCL_0597 denotes Ramos