In the developing brain we noted a striking change in the subcellular distribution of Annexin A7. Cells in the stratum germinativum of the neopallial cortex, which surrounds the lateral ventricle, show at E13 and E15 a staining for Annexin A7 mainly in the cytosol. But this staining largely disappears and at the following day E16 we detect Annexin A7 in the nucleus of cells in the intermediate and marginal zone of the neopallium. The different expression patterns of Annexin A7-positive cells from the ventricular germinative zone to the marginal zone of the later neocortex observed in this study are similar to the developmental patterns of Tenascin-C-positive astroglial precursors following the guidance of the radial glial cells [18]. Moreover, the patterns resemble that of migrating and differentiated neurons described by Berry et al. [13]. Neurons that are generated prenatally in the proliferative ventricular layer of the neopallial cortex subsequently migrate through the intermediate zone to form the different cortical cell layers in a declining inside-out gradient of cell maturation. These observations suggested that the subcellular localization of Annexin A7 depends on developmental stage and cell type.