Both Annexin A7 isoforms are found in adult brain tissue (Fig. 1B). In general, we observed two characteristic staining patterns for Annexin A7, a prominent nuclear stain in neurons (determined by lack of GFAP-immunoreactivity, localization, morphology) and a cytoplasmic and nuclear stain in astrocytes (GFAP-positive) in all areas of the mature murine brain. In the neocortex (isocortex) Annexin A7 was strongly enriched in nuclei of neurons of all six cortical laminae (Fig. 4A, section derived from cortex temporalis). For control, in the adult brain type-1 astrocytes can be characterized by a positive GFAP-stain (Fig. 4B). Fig. 4F demonstrates the presence of nuclear Annexin A7 in a neocortical neuron of the external granular layer (layer II) and in an astrocytic cell of the neocortical molecular layer (layer I) which additionally exhibits an intense signal in the cytoplasm and in cellular branches. A further cell type of the isocortex showed a strong immunoreactivity in both, nucleus and cytosol, and was identified as pyramidal neuron based on morphology and absence of GFAP immunoreactivity. However, Annexin A7 apparently is more enriched in the nucleus (Fig. 4E).