Supporting Information
Figure S1  Activity Levels in Female PGC-1α−/− Mice Is Unchanged
Using a CLAMS system, PGC-1α+/+ (n = 4) and PGC-1α−/− (n = 3) female mice were monitored for 48 h after a 17-h period of acclimation. XY beam breaks were tabulated over the 12-h light and dark cycles as denoted on the bottom. The bars represent mean (± SEM) beam breaks per each 12-h cycle.
(342 KB EPS).
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Figure S2  Altered Emotionality in PGC-1α−/− Mice
An analysis of exploratory behavior included the number of entries into the center of the cage (upper left), the time spent in the center of the cage in seconds (sec) (upper right), the distance traveled in the center of the cage in meters (m) (lower left) as well as the distance traveled in the periphery (lower right). * p < 0.05 compared to the PGC-1α+/+ mice.
(620 KB EPS).
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Figure S3  No Difference in Weight Gain on a High-Fat Diet in Female PGC-1α−/− Mice Compared to WT Controls
8-wk-old female mice were fed a diet high in fat (43% calories from fat) for 6 wk. The change in body weight (grams) after 6 wk on a high-fat diet is shown for PGC-1α+/+ (n = 8) and PGC-1α−/− (n = 11) mice. NS, not significant.
(264 KB EPS).
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Figure S4  Male PGC-1α−/− Mice Are Somewhat Resistant to Diet-Induced Obesity
Male and female PGC-1α+/+ (n ≥ 6) and PGC-1α−/− (n ≥ 6) mice were fed a high-fat diet (43% calories from fat) beginning at 4 wk of age. Body weight was monitored weekly as shown on the graph on the left. The mean (± SEM) change in body weight is shown in the bar graph on the right. *, significant difference compared to the PGC-1α+/+ controls, p < 0.05.
(794 KB EPS).
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Table S1  Probes and Primers
Sequences of mouse-specific probes and primers used for real-time RT-PCR.
(25 KB DOC).
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Accession Numbers
The GenBank (http://www.ncbi.nlm.nih.gov/) accession numbers of the vector discussed in this paper is p1339-PGK-Neomycin targeting vector (AF335420).