Figure 3 Model Construction Population model includes individual size-specific growth and mortality rates, and population size-specific recruitment rate. Growth rate was determined by in situ staining of tubeworm aggregations using a blue chitin stain (in situ photograph of stained aggregation demonstrating annual growth shown here) and collection after 12–14 mo. Diagenetic model included advection and diffusion of sulfate, sulfide, methane, bicarbonate, and hydrogen ions as well as organic carbon content of sediments. Fluxes across the rhizosphere (root system) boundary were compared to sulfide uptake rates for simulated aggregations to determine whether sulfide supply could match the required uptake rates of aggregations (for specific methodology see methods). HC, C6+ hydrocarbons; orgC, organic carbon; ox, oxidation reaction; red, reduction reaction.