Table 1  Kinetic parameters for the hydrolysis of CoA and CoA derivatives by Trx-Y87G2A.14 fusion protein
Substrate  Km (mM)  kcat (s-1)  kcat / Km (103M-1.s-1)
CoA  0.22  13.8  64
Acetyl-CoA  0.23  11.8  51
Succinyl-CoA  0.24  12.7  53
3-Hydroxymethylglutaryl-CoA  0.20  10.5  54
CoASSCoA  0.32  2.9  9.1
Data were collected using the HPLC assay described in Materials and methods.