PMC:101390 / 21041-22498 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/101390","sourcedb":"PMC","sourceid":"101390","source_url":"https://www.ncbi.nlm.nih.gov/pmc/101390","text":"Results are shown in Figure 5. Concerning the enzyme extracted from cells, partition coefficient reaches an optimum at 3% w/v detergent, decreasing at higher detergent concentrations. We do not have direct evidence to explain this decrease but a reason for it could be a change in the composition of the rich phase that may contain more components of the bacterial cell wall extracted at high detergent concentration, thus interfering in the partitioning of COX. In the case of the extracellular COX the higher the detergent concentration the larger the partition coefficient. As seen in table 4, the rich phase is quite more compact after phase separation in the culture broth than in the cell-extract and its relative volume is not proportional to the detergent concentration. Thus the rich phase is less hydrated, that is, more hydrophobic, with the culture broth than with the cell-extract and the more hydrophobic the higher the detergent concentration. As a result the partition coefficient for COX in the culture broth increases with the detergent concentration. Phase separation of Triton X-114 is affected by the presence of other surfactants such as Triton X-45 [28] and polyols such as glycerol [29], thus bacterial surfactants extracted during detergent treatment may affect phase separation as well. Natural surfactants may be also present in the broth but at a much lower concentration, therefore having less influence on the phase separation.","tracks":[{"project":"2_test","denotations":[{"id":"11914155-1645934-8561979","span":{"begin":1173,"end":1175},"obj":"1645934"},{"id":"11914155-1952054-8561980","span":{"begin":1207,"end":1209},"obj":"1952054"}],"attributes":[{"subj":"11914155-1645934-8561979","pred":"source","obj":"2_test"},{"subj":"11914155-1952054-8561980","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#ec93c3","default":true}]}]}}