Table 2 shows the amount and the percentage of cell-linked COX extracted by either Triton X-100 or Triton X-114 at several detergent concentrations. COX could be extracted from cells and solubilized by both detergents at 1% w/v. The % of recovery is of the same order as previously reported for these detergents [17]. The increase of the Triton X-114 concentration from 1 to 3% w/v resulted in concomitant increases of 1.5-fold the total activity, 2.6-fold the specific activity and a % of recovery above 90%. A Triton X-114 concentration as high as 6% w/v does not improve significantly the recovery nor the specific activity. Phase separation was further induced to gain COX purity in extracts and in the broth independently.