|
Abstract
Supplementary Figure 1. Antigen expression after infection with MeVac encoding TRP-2 or OVA. Vero cells were subjected to mock infection or infected with MeVac encoding the TRP-2 or OVA antigen at MOI 3. After 48 h, cells were lyzed and Western blot was performed using antigen-specific antisera. Lane 1: mock; lane 2: MeVac TRP-2, MeVac OVA; lane 3: positive control (TRP-2: melanosomes; OVA: chicken egg white). Supplementary Figure 2. Replication kinetics of measles vaccine viruses encoding antigens. To generate one-step growth curves, Vero cells were inoculated with MeVac variants at MOI 3 in triplicates. At designated time points, cells were harvested, triplicate samples were pooled and viral progeny were quantified by titration assays. Supplementary Figure 3. The measles Schwarz vaccine strain infects MC38-hCD46 cells. MC38-hCD46 cells were subjected to mock infection or inoculated with MeVac encoding eGFP. Images were acquired 24 and 48 h post infection (p.i.). Scale bars: 200 µm.
MC38-hCD46 cells were infected with indicated MeVac variants at MOI 3. After 24 h, cells were lyzed by one freeze-thaw cycle. As control, MC38-hCD46 cells were loaded with SIINFEKL peptide and then lyzed (MC38-hCD46 SIINFEKL). Lysates were cleared by centrifugation. DC2.4 cells were inoculated with lysates or pulsed with SIINFEKL peptide for one hour (bottom row, right panel) and flow cytometry for SIINFEKL bound to H-2K b was performed with a PE-labeled antibody.
|
