ACL LDT HIVING produced 90% clinical sensitivity (18/19) and specificity (19/21) all samples were genotype M, subtype B (40) with viral loads ranging from (1000 to 150,000 cp/mL), analytical sensitivity 100% at VL=1000 cp/mL for HIV subtypes (B, G, C, CRF01_AE) and per in silico analysis HIV genotypes (D, F, A, CRF02_AG), analytical specificity 100% (11/11) in comparison to potentially cross-reactive microorganisms/viruses.