The primer sequences for ANA are: forward 5'-CAG TCG TGA ATG TAG AGG GAA AAA C-3'; reverse 5'-GGA ATC CCC CTT CAG GAA CTT G-3' and for EHR are: forward 5'-AAT GCT TCT ACT GCT ACT GT-3'; reverse 5'-GCT CCA CCA TGA GCT GG-3'.