CORD-19:e9900f49f3469114a7ad6b81b7692540cdf17817 / 20619-20902 JSONTXT

{"project":"CORD-19_Custom_license_subset","denotations":[{"id":"T117","span":{"begin":0,"end":283},"obj":"Sentence"}],"text":"Briefly, radiolabeled cell lysates (1 ϫ 10 5 cell equivalents) were diluted in 1 ml RIPA buffer (0.5% Triton X-100, 0.1% SDS, 300 mM NaCl, 4 mM EDTA, and 50 mM Tris-HCl, pH 7.4) and immunoprecipitated with 2 l of nsp-specific antibodies and protein A Sepharose beads (GE Healthcare)."}

{"project":"CORD-19-Sentences","denotations":[{"id":"TextSentencer_T117","span":{"begin":0,"end":283},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Briefly, radiolabeled cell lysates (1 ϫ 10 5 cell equivalents) were diluted in 1 ml RIPA buffer (0.5% Triton X-100, 0.1% SDS, 300 mM NaCl, 4 mM EDTA, and 50 mM Tris-HCl, pH 7.4) and immunoprecipitated with 2 l of nsp-specific antibodies and protein A Sepharose beads (GE Healthcare)."}