Glove reuse should be avoided, but if it occurs in situations with limited resources, the following steps should be performed: (1) evaluation process to verify the reasons and need for reuse; (2) safe handling of contaminated gloves by healthcare workers during reprocessing should be guaranteed; (3) selection process: only surgical latex gloves can be reused for examination purposes and/or for surgical double-gloving; (4) reprocessing: decontamination with 0.5% chlorine solution, cleansing with soap and water, autoclave sterilization or steam high-level disinfection, and integrity test with air or water filling.