A cluster of pyrazolone analogues were screened and evaluated for the anti-ALS activity from FDA approved drugs and biochemical reagents by Radhia and co-workers [73] , as the most potent skeleton, the arylsulfanyl pyrazolones showed 100% efficacy compared with the positive control, radicicol (80% efficacy) in the test of G93A-SOD1 expression in PC12 cells model.