For F. necrophorum, a selective anaerobic plate (fastidious anaerobe agar (Lab M) with 5% horse blood (SVA) supplemented with vancomycin 2.5 mg/L (ICN Biomedicals, Irvine, CA, USA) and nalidixic acid 5.0 mg/L (MP Biomedicals, Santa Ana, CA, USA)), and with a kanamycin tablet 500 μg (Rosco, Taastrup, Denmark) was used [21] .