In the present study we decided to evaluate a distribution of virulence genes encoding for cytotoxic necrotizing factor type 1 (cnf1), P-fimbriae, S/F1C-fimbriae aerobactin (aer), and afa genes in alpha-haemolytic E. coli strains isolated from gynaecological material in our region and to compare the detected sequences in clinical isolates of other diagnostic groups.