Production and cryo-EM of unilamellar phospholipid vesicles (SUV 100 ) created from a blend of 80 parts 1,2-dioleoyl-sn-glycero-3-phosphocholine, 10 parts 1,2-dioleoyl-snglycero-3-[phosphor-rac-(1-glycerol)], 2 parts 1,2-distearoyl-sn-glycero-3phosphoethanolamine-N-[biotinyl(polyethylene glycol)2000], and 1 part 3,3'-dioctadecyloxacarbocyanine perchlorate has been described previously (Kunding et al., 2008) .