One million cells were incubated with anti-Fc blocking antibody (anti-mouse FcRεII/III, 24G2) for 15 min at 4 • C and incubated with titered dilutions of the following antibodies: CD4-FITC (RMA4-5), CD8-PE (53-6.7), CD45-PerCP (Ly-5), and CD11b-APC (M1/70), CD19-PE (1D3), and CD3-APC (145-2C11) (all antibodies from Pharmingen, San Diego, CA).