These data support the clinical results ( Figure 4) where disease onset is more rapid in the CCL2-transgenic mice compared to control mice. incubation with anti-CD45 and anti-CD11b antibodies and flow cytometric sorting in to CD45 low CD11b + and CD45 high CD11b + pools. (A) Purified T cells were incubated with antibodies to CD62L, CD25, CD45, and TcRα/β and analyzed by flow cytometry.