We evaluated four different automated syphilis serological tests, including Architect Syphilis TP ® (Abbott Diagnostics, Tokyo, Japan), Lumipulse ® presto TP (Fujirebio Inc, Tokyo, Japan), Serodia ® -TP·PA (Fujirebio INC, Tokyo, Japan), Esplain ® TP (Fujirebio Inc, Tokyo, Japan).