Similarly, a range of nucleic acid concentrations that included high (5.5 × 10 3 gc/250 μL CSF equivalent to 5000 gc/reaction), medium (5.5 × 10 2 gc/250 μL CSF equivalent to 500 gc/reaction), and low (550 gc/250 μL CSF equivalent to 50 gc/reaction) were selected for the EEEV transcript.