In addition, Far Western blotting experiments mapped Embp fibronectin interactions to FnIII 12 -13 domains By making use of Embp expressing S. epidermidis strain 1585v and isogenic, Embp-negative mutant 1585v-M135 the functional relevance of Embp-Fn interactions for S. epidermidis surface colonization was then tested in semi-quantitative adherence assays, showing that Embp-expression is sufficient and necessary for S. epidermidis binding to surface immobilized Fn.