However, a whole virus-based ELISA and Western blot showed two-way antigenic cross-reactivities between PEDV VBS2 strain and PDCoV Michigan/8977/2014 strain (Ma et al., 2016) . Sequence analysis of the N proteins showed 4 motifs (47- are conserved between PEDV and PDCoV, probably leading to the cross-reactivities in the immunoassays.