The results from 422 qRT-PCR assays and western blot analyses showed that, compared with that of the 423 control, the expression of PI3K and phosphorylation levels of Akt and mTOR (p-Akt 424 and p-mTOR) in the HSV-2-infected cells were significantly increased, and these 425 proteins all were decreased significantly in the JZ-1+HSV-2 group (Fig. 5B, Fig. 7C ). showed that, compared with that of the HSV-2 group, the expression levels of PI3K, 433 p-Akt and p-mTOR were significantly reduced in the LY + HSV-2 group, indicating 434 that it indeed inhibited the PI3K/Akt/ mTOR pathway (Fig. 6C, Fig. 7D ).