One H5 subtype-specific monoclonal antibody (mAb) (2H2) (detection) was developed by Professor Ho-Joon Shin (Ajou University, Suwon, Korea) and Professor Hyun Park (Wonkwnag University, Iksan, Korea); H5 subtype-specific mAb (1C5) (capture) was donated by Dr. Chom-Kyu Chong (GenBody Inc., Cheonan, Korea).