The highest concentrations of bioconjugates (H5 HA conjugate: influenza A conjugate=50:50) not only interfered with migration on the strips, but also increased the relative CL value decreasing the TL/CL ratio, indicating that there is no significant difference between the negative sample and H5N3 (20 HAU/mL) in terms of 'fold of control' (Fig. 5A) .