Another DNA fragment containing a 12-bp sequence of a Bgl II site and additional 6 bp, a sequence encoding the signal peptide, anti-HIV-1 gp41 scFv (TG15) and 11 residues of human IgG3 hinge region, and an 8-bp sequence of the 5'end of the his tag was amplified by PCR using previously generated pLNCX-TCR-m-scFv as a template [23] .