BB-kb@ldeleger:BB-kb-18346136 / 227-1229 JSONTXT

{"project":"bionlp-ost-19-BB-kb-test","denotations":[{"id":"T3","span":{"begin":0,"end":1002},"obj":"Paragraph"},{"id":"T10","span":{"begin":86,"end":108},"obj":"Habitat"},{"id":"T11","span":{"begin":133,"end":161},"obj":"Habitat"},{"id":"T12","span":{"begin":194,"end":204},"obj":"Microorganism"},{"id":"T13","span":{"begin":359,"end":370},"obj":"Microorganism"},{"id":"T14","span":{"begin":380,"end":415},"obj":"Habitat"},{"id":"T15-0","span":{"begin":380,"end":405},"obj":"_FRAGMENT"},{"id":"T15-1","span":{"begin":420,"end":434},"obj":"Habitat"},{"id":"T16","span":{"begin":565,"end":600},"obj":"Habitat"},{"id":"T17","span":{"begin":877,"end":915},"obj":"Habitat"}],"relations":[{"id":"C-T15-0","pred":"_lexicallyChainedTo","subj":"T15-1","obj":"T15-0"}],"text":"The method consisted of a novel two-step enrichment involving overnight incubation in buffered peptone water and a 5-h subculture in Rappaport-Vassiliadis medium, lysis of bacterial cells and a Salmonella-specific 5'-nuclease real-time PCR with an exogenous internal amplification control. Because a two-step enrichment was used, the detection limit for dead S. enterica cells in artificially contaminated ice cream and salami samples was high at 10(7 )CFU (25 g)(-1), eliminating potential false-positive results. When the method was evaluated with a range of 100 naturally contaminated food samples, three positive samples were detected by both the real-time PCR-based method and by the standard microbiological method, according to EN ISO 6579. When the real-time PCR-based method was evaluated alongside the standard microbiological method according to EN ISO 6579 with 36 food samples artificially contaminated at a level of 10(0 )CFU (25 g)(-1), identical results were obtained from both methods."}