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PMC:149366 JSONTXT

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div. section text # proj. length # Ann.
0 TIAB Brn3c null mutant mice show long-term, incomplete retention of some afferent inner ear innervation B 5 1746 53 show
1 Background Brn3c is a POU domain factor that is crucial for inner ear hair cell development. Targeted null Brn3 5 3026 92 show
2 Results To appreciate the effects of the Brn3c null mutation on the pattern of the inner ear innervation, we 5 751 18 show
3 Results Brn3c null mutants at birth (P0) Vestibular ganglia are smaller in Brn3c null mutants (Fig. 1b) than 5 2993 111 show
4 Results Brn3c null mutants at P7/8 Reduction of vestibular innervation is more pronounced than at P0. Loss o 5 4017 138 show
5 Results 6 months Brn3c null mutants Very few fibers (both afferent and efferent) to the vestibular epithelia 5 1619 44 show
6 Results In situ hybridization confirms neonatal neurotrophin expression We processed four ears of P0 and P8 5 1903 74 show
7 Discussion We will explore five points in this discussion: How the limited expression of neurotrophins relates 5 496 11 show
8 Discussion Immature hair cells and limited expression of neurotrophins rescue afferent projection until P8 The 5 3272 98 show
9 Discussion Long term survival of apical sensory neurons is unrelated to differentiated hair cells and likely is 5 1628 42 show
10 Discussion Growth of fibers to the cochlea does not require mature hair cells Formation of radial fibers that b 5 2980 67 show
11 Discussion Relevance of our findings to other mutations with hair cell loss Vahava et al. [12] and Frydman et a 5 1285 30 show
12 Conclusion The progressive loss of afferent and efferent innervation in Brn3c null mutants shows neither in spa 5 1094 27 show
13 Methods Breeding and genotyping Brn3c mice were bred as previously described [22]. Mice were genotyped and B 5 431 9 show
14 Methods Tracing of nerve fibers DiI tracing from the brainstem was performed as previously described [41]. S 5 1785 29 show
15 Methods In situ hybridization Four ears each of Brn3c null mutants (P0 and P8) and control littermates were 5 427 5 show
16 Methods Immunocytochemistry of hair cells and quantification of hair cells and neurons Immunostaining of P7 5 925 21 show
17 Caption-Figure 1 Size variations of vestibular ganglia in control and mutant littermates labeled with DiI. In newborn 5 713 36 show
18 Caption-Figure 2 Innervation of Brn3c null and control ears are shown for newborn mice. There is no specific loss of 5 621 13 show
19 Caption-Figure 3 Reduction of innervation of the ear is shown in 8-day old mice. While all vestibular sensory epithel 5 835 14 show
20 Caption-Figure 4 The distribution of hair cells as identified with MyoVII immunocytochemistry is shown for 7-day old 5 1329 29 show
21 Caption-Figure 5 This graph shows the reduction in sensory neurons (top) and in hair cells (bottom) between 7-day old 5 538 12 show
22 Caption-Figure 6 These confocal images show the projection pattern of a P8 Brn3c null mutant mouse. DiI (red) was ins 5 616 15 show
23 Caption-Figure 7 The innervation of sensory epithelia is shown in 6-month old Brn3c null mutant mice. Compared to P8 5 626 16 show
24 Caption-Figure 8 The expression of BDNF and NT-3 mRNA is shown in the P0 vestibular sensory epithelia. Silver grains 5 619 23 show
25 Caption-Figure 9 BDNF and NT-3 mRNA expression is shown in the cochlea of P0 (a-d) and P8 (e,f) Brn3c null and contro 5 548 16 show
26 Caption-Table 1 Numbers of hair cells and sensory neurons in P7 wildtype and Brn3c mutant littermates 5 85 3 show