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PMC:116589 JSONTXT

Divisions (13) JSON TSV

div. section text # proj. length # Ann.
0 TIAB Embryonic stem cells and mice expressing different GFP variants for multiple non-invasive reporter u 5 2132 38 show
1 Background The ease of manipulating its genome has helped establish the mouse as the premier model organism for 5 2025 29 show
2 Results and discussion We tested several of the available wtGFP variants for developmental neutrality and stability of wide 5 6539 125 show
3 Conclusions From these observations we conclude that the blue-shifted ECFP, and the red-shifted EYFP variants of 5 2025 20 show
4 Materials and methods Vector construction cDNAs encoding ECFP, EYFP and DsRed were amplified from plasmids pECFP, pEYFP an 5 1130 37 show
5 Materials and methods Tissue culture R1 ES cells [20] were maintained under standard culture conditions in the presence of 5 761 8 show
6 Materials and methods Chimera production and germline transmission ES cell lines exhibiting robust transgene expression in 5 817 13 show
7 Materials and methods Imaging Photographs of live samples, freshly dissected in PBS ([24] were taken on a Leica MZFLIII st 5 842 2 show
8 Materials and methods Footnote The cyan and yellow fluorescent protein-expressing mice described here have been acquired b 5 299 2 show
9 Caption-Figure 1 Non-invasive multiple reporter visualization in ES cells. (a-d) a mixture of CK6/ECFP (ECFP+) and YC 5 564 3 show
10 Caption-Figure 2 Dual non-invasive reporter visualization in embryos and adult mice. Transgenic animals are hemizygou 5 1656 20 show
11 Caption-Figure 3 Dual non-invasive reporter visualization in chimeric embryos generated by tetraploid <-> diploid agg 5 1892 30 show
12 Caption-Figure 4 Dual non-invasive reporter visualization in the organs of adult chimeras. All images depict dark fie 5 1563 33 show