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[Studies on the suppressor cells acting on the mitogen-induced blastogenesis in tumor-bearing hosts. I. Suppressor cells in the spleens and peripheral blood of tumor-bearing rats (author's transl)].
When Walker carcinosarcoma 256 cells were transplanted to the inbred Fischer male rats, the responses of spleen cells to Concanavalin A (Con A) or Phytohemagglutinin (PHA) stimulation were gradually reduced as tumor grew up. However, these responses were significantly restored in most cases when the phagocytic or adherent cells were removed from spleen cell suspensions by treating them with a carbonyl iron-magnetic procedure or the passing through a Sephadex G-10 column. Similarly, the decreased responses of peripheral blood mononuclear cells from tumor-bearing rats to Con A stimulation were also restored by removal of the adherent cells with a Sephadex G-10 column. These results strongly suggest a possibility that the suppressor cells, probably the monocyte-macrophage series, were contained in both spleen cells and peripheral blood mononuclear cells and these cells exert their suppressive effect on the mitogen-induced lymphocyte transformation.
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