Id |
Subject |
Object |
Predicate |
Lexical cue |
T1 |
0-139 |
Sentence |
denotes |
[Role of 14-3-3σgene in the regulation of endotoxin/lipopolysaccharide-induced inflammatory responses in human pulmonary epithelial cells]. |
T2 |
140-150 |
Sentence |
denotes |
Objective: |
T3 |
151-309 |
Sentence |
denotes |
To explore the mechanism of 14-3-3σgene in regulating inflammatory response of human pulmonary epithelial cells induced by endotoxin/lipopolysaccharide (LPS). |
T4 |
310-549 |
Sentence |
denotes |
Methods: (1) Cells of human normal pulmonary epithelial cell line BEAS-2B cultured in logarithmic growth period were collected and divided into control group and PCMV6-14-3-3σgroup using the random number table, with 3 wells in each group. |
T5 |
550-685 |
Sentence |
denotes |
Cells in control group were transfected with empty plasmid, and cells in PCMV6-14-3-3σgroup were transfected with PCMV6-14-3-3σplasmid. |
T6 |
686-1060 |
Sentence |
denotes |
The protein expression of 14-3-3σin cell was detected by Western blotting at 48 hours after transfection. (2) Cells of human normal pulmonary epithelial cell line BEAS-2B cultured in logarithmic growth period were collected and divided into control group, PCMV6-14-3-3σgroup, PCMV6-14-3-3σ+ LPS group, and LPS group using the random number table, with 3 wells in each group. |
T7 |
1061-1133 |
Sentence |
denotes |
Cells in control group were transfected with empty plasmid for 42 hours. |
T8 |
1134-1218 |
Sentence |
denotes |
Cells in PCMV6-14-3-3σgroup were transfected with PCMV6-14-3-3σplasmid for 42 hours. |
T9 |
1219-1403 |
Sentence |
denotes |
Cells in PCMV6-14-3-3σ+ LPS group were stimulated with 1 μg/mL LPS (the same final mass concentration below) for 6 hours after being transfected with PCMV6-14-3-3σplasmid for 42 hours. |
T10 |
1404-1458 |
Sentence |
denotes |
Cells in LPS group were stimulated by LPS for 6 hours. |
T11 |
1459-1600 |
Sentence |
denotes |
The protein expressions of Bax and B-cell lymphoma-2 (Bcl-2) were detected by Western blotting, and the ratio of Bax to Bcl-2 was calculated. |
T12 |
1601-1647 |
Sentence |
denotes |
Apoptotic rate was detected by flow cytometry. |
T13 |
1648-1857 |
Sentence |
denotes |
The mRNA expressions of tumor necrosis factor alpha (TNF-α) and interleukin 1beta (IL-1β) in cells were detected by real-time fluorescent quantitative reverse transcription polymerase chain reaction technique. |
T14 |
1858-1963 |
Sentence |
denotes |
Content of TNF-α and IL-1β in cell culture supernatant was detected by enzyme-linked immunosorbent assay. |
T15 |
1964-2078 |
Sentence |
denotes |
Data were statistically analyzed with t test, one-way analysis of variance, and least significant difference test. |
T16 |
2079-2617 |
Sentence |
denotes |
Results: (1) At 48 hours after transfection, the protein expression of 14-3-3σin cells of PCMV6-14-3-3σgroup (1.05±0.03) was significantly higher than that in control group (0.78±0.04, t=5.41, P<0.01). (2) Compared with those in control group, the ratio of Bax to Bcl-2, apoptotic rate, mRNA expressions of TNF-α and IL-1β, and content of TNF-α and IL-1β in cell supernatant in PCMV6-14-3-3σgroup showed no significant difference (P>0.05); the above-mentioned indexes of cells in LPS group were significantly higher or increased (P<0.01). |
T17 |
2618-2764 |
Sentence |
denotes |
Compared with those in LPS group, the above-mentioned indexes of cells in PCMV6-14-3-3σ+ LPS group were significantly lower or decreased (P<0.01). |
T18 |
2765-2777 |
Sentence |
denotes |
Conclusions: |
T19 |
2778-2825 |
Sentence |
denotes |
14-3-3σis a key factor in regulating apoptosis. |
T20 |
2826-3004 |
Sentence |
denotes |
It can alleviate the LPS-induced inflammatory responses by regulating the ratio of apoptotic regulators Bax to Bcl-2 and inhibiting apoptosis of human pulmonary epithelial cells. |
T1 |
0-139 |
Sentence |
denotes |
[Role of 14-3-3σgene in the regulation of endotoxin/lipopolysaccharide-induced inflammatory responses in human pulmonary epithelial cells]. |
T2 |
140-150 |
Sentence |
denotes |
Objective: |
T3 |
151-309 |
Sentence |
denotes |
To explore the mechanism of 14-3-3σgene in regulating inflammatory response of human pulmonary epithelial cells induced by endotoxin/lipopolysaccharide (LPS). |
T4 |
310-549 |
Sentence |
denotes |
Methods: (1) Cells of human normal pulmonary epithelial cell line BEAS-2B cultured in logarithmic growth period were collected and divided into control group and PCMV6-14-3-3σgroup using the random number table, with 3 wells in each group. |
T5 |
550-685 |
Sentence |
denotes |
Cells in control group were transfected with empty plasmid, and cells in PCMV6-14-3-3σgroup were transfected with PCMV6-14-3-3σplasmid. |
T6 |
686-1060 |
Sentence |
denotes |
The protein expression of 14-3-3σin cell was detected by Western blotting at 48 hours after transfection. (2) Cells of human normal pulmonary epithelial cell line BEAS-2B cultured in logarithmic growth period were collected and divided into control group, PCMV6-14-3-3σgroup, PCMV6-14-3-3σ+ LPS group, and LPS group using the random number table, with 3 wells in each group. |
T7 |
1061-1133 |
Sentence |
denotes |
Cells in control group were transfected with empty plasmid for 42 hours. |
T8 |
1134-1218 |
Sentence |
denotes |
Cells in PCMV6-14-3-3σgroup were transfected with PCMV6-14-3-3σplasmid for 42 hours. |
T9 |
1219-1403 |
Sentence |
denotes |
Cells in PCMV6-14-3-3σ+ LPS group were stimulated with 1 μg/mL LPS (the same final mass concentration below) for 6 hours after being transfected with PCMV6-14-3-3σplasmid for 42 hours. |
T10 |
1404-1458 |
Sentence |
denotes |
Cells in LPS group were stimulated by LPS for 6 hours. |
T11 |
1459-1600 |
Sentence |
denotes |
The protein expressions of Bax and B-cell lymphoma-2 (Bcl-2) were detected by Western blotting, and the ratio of Bax to Bcl-2 was calculated. |
T12 |
1601-1647 |
Sentence |
denotes |
Apoptotic rate was detected by flow cytometry. |
T13 |
1648-1857 |
Sentence |
denotes |
The mRNA expressions of tumor necrosis factor alpha (TNF-α) and interleukin 1beta (IL-1β) in cells were detected by real-time fluorescent quantitative reverse transcription polymerase chain reaction technique. |
T14 |
1858-1963 |
Sentence |
denotes |
Content of TNF-α and IL-1β in cell culture supernatant was detected by enzyme-linked immunosorbent assay. |
T15 |
1964-2078 |
Sentence |
denotes |
Data were statistically analyzed with t test, one-way analysis of variance, and least significant difference test. |
T16 |
2079-2617 |
Sentence |
denotes |
Results: (1) At 48 hours after transfection, the protein expression of 14-3-3σin cells of PCMV6-14-3-3σgroup (1.05±0.03) was significantly higher than that in control group (0.78±0.04, t=5.41, P<0.01). (2) Compared with those in control group, the ratio of Bax to Bcl-2, apoptotic rate, mRNA expressions of TNF-α and IL-1β, and content of TNF-α and IL-1β in cell supernatant in PCMV6-14-3-3σgroup showed no significant difference (P>0.05); the above-mentioned indexes of cells in LPS group were significantly higher or increased (P<0.01). |
T17 |
2618-2764 |
Sentence |
denotes |
Compared with those in LPS group, the above-mentioned indexes of cells in PCMV6-14-3-3σ+ LPS group were significantly lower or decreased (P<0.01). |
T18 |
2765-2777 |
Sentence |
denotes |
Conclusions: |
T19 |
2778-2825 |
Sentence |
denotes |
14-3-3σis a key factor in regulating apoptosis. |
T20 |
2826-3004 |
Sentence |
denotes |
It can alleviate the LPS-induced inflammatory responses by regulating the ratio of apoptotic regulators Bax to Bcl-2 and inhibiting apoptosis of human pulmonary epithelial cells. |