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PubMed:32340415 JSONTXT

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Inflammaging

Id Subject Object Predicate Lexical cue
T1 0-139 Sentence denotes [Role of 14-3-3σgene in the regulation of endotoxin/lipopolysaccharide-induced inflammatory responses in human pulmonary epithelial cells].
T2 140-150 Sentence denotes Objective:
T3 151-309 Sentence denotes To explore the mechanism of 14-3-3σgene in regulating inflammatory response of human pulmonary epithelial cells induced by endotoxin/lipopolysaccharide (LPS).
T4 310-549 Sentence denotes Methods: (1) Cells of human normal pulmonary epithelial cell line BEAS-2B cultured in logarithmic growth period were collected and divided into control group and PCMV6-14-3-3σgroup using the random number table, with 3 wells in each group.
T5 550-685 Sentence denotes Cells in control group were transfected with empty plasmid, and cells in PCMV6-14-3-3σgroup were transfected with PCMV6-14-3-3σplasmid.
T6 686-1060 Sentence denotes The protein expression of 14-3-3σin cell was detected by Western blotting at 48 hours after transfection. (2) Cells of human normal pulmonary epithelial cell line BEAS-2B cultured in logarithmic growth period were collected and divided into control group, PCMV6-14-3-3σgroup, PCMV6-14-3-3σ+ LPS group, and LPS group using the random number table, with 3 wells in each group.
T7 1061-1133 Sentence denotes Cells in control group were transfected with empty plasmid for 42 hours.
T8 1134-1218 Sentence denotes Cells in PCMV6-14-3-3σgroup were transfected with PCMV6-14-3-3σplasmid for 42 hours.
T9 1219-1403 Sentence denotes Cells in PCMV6-14-3-3σ+ LPS group were stimulated with 1 μg/mL LPS (the same final mass concentration below) for 6 hours after being transfected with PCMV6-14-3-3σplasmid for 42 hours.
T10 1404-1458 Sentence denotes Cells in LPS group were stimulated by LPS for 6 hours.
T11 1459-1600 Sentence denotes The protein expressions of Bax and B-cell lymphoma-2 (Bcl-2) were detected by Western blotting, and the ratio of Bax to Bcl-2 was calculated.
T12 1601-1647 Sentence denotes Apoptotic rate was detected by flow cytometry.
T13 1648-1857 Sentence denotes The mRNA expressions of tumor necrosis factor alpha (TNF-α) and interleukin 1beta (IL-1β) in cells were detected by real-time fluorescent quantitative reverse transcription polymerase chain reaction technique.
T14 1858-1963 Sentence denotes Content of TNF-α and IL-1β in cell culture supernatant was detected by enzyme-linked immunosorbent assay.
T15 1964-2078 Sentence denotes Data were statistically analyzed with t test, one-way analysis of variance, and least significant difference test.
T16 2079-2617 Sentence denotes Results: (1) At 48 hours after transfection, the protein expression of 14-3-3σin cells of PCMV6-14-3-3σgroup (1.05±0.03) was significantly higher than that in control group (0.78±0.04, t=5.41, P<0.01). (2) Compared with those in control group, the ratio of Bax to Bcl-2, apoptotic rate, mRNA expressions of TNF-α and IL-1β, and content of TNF-α and IL-1β in cell supernatant in PCMV6-14-3-3σgroup showed no significant difference (P>0.05); the above-mentioned indexes of cells in LPS group were significantly higher or increased (P<0.01).
T17 2618-2764 Sentence denotes Compared with those in LPS group, the above-mentioned indexes of cells in PCMV6-14-3-3σ+ LPS group were significantly lower or decreased (P<0.01).
T18 2765-2777 Sentence denotes Conclusions:
T19 2778-2825 Sentence denotes 14-3-3σis a key factor in regulating apoptosis.
T20 2826-3004 Sentence denotes It can alleviate the LPS-induced inflammatory responses by regulating the ratio of apoptotic regulators Bax to Bcl-2 and inhibiting apoptosis of human pulmonary epithelial cells.
T1 0-139 Sentence denotes [Role of 14-3-3σgene in the regulation of endotoxin/lipopolysaccharide-induced inflammatory responses in human pulmonary epithelial cells].
T2 140-150 Sentence denotes Objective:
T3 151-309 Sentence denotes To explore the mechanism of 14-3-3σgene in regulating inflammatory response of human pulmonary epithelial cells induced by endotoxin/lipopolysaccharide (LPS).
T4 310-549 Sentence denotes Methods: (1) Cells of human normal pulmonary epithelial cell line BEAS-2B cultured in logarithmic growth period were collected and divided into control group and PCMV6-14-3-3σgroup using the random number table, with 3 wells in each group.
T5 550-685 Sentence denotes Cells in control group were transfected with empty plasmid, and cells in PCMV6-14-3-3σgroup were transfected with PCMV6-14-3-3σplasmid.
T6 686-1060 Sentence denotes The protein expression of 14-3-3σin cell was detected by Western blotting at 48 hours after transfection. (2) Cells of human normal pulmonary epithelial cell line BEAS-2B cultured in logarithmic growth period were collected and divided into control group, PCMV6-14-3-3σgroup, PCMV6-14-3-3σ+ LPS group, and LPS group using the random number table, with 3 wells in each group.
T7 1061-1133 Sentence denotes Cells in control group were transfected with empty plasmid for 42 hours.
T8 1134-1218 Sentence denotes Cells in PCMV6-14-3-3σgroup were transfected with PCMV6-14-3-3σplasmid for 42 hours.
T9 1219-1403 Sentence denotes Cells in PCMV6-14-3-3σ+ LPS group were stimulated with 1 μg/mL LPS (the same final mass concentration below) for 6 hours after being transfected with PCMV6-14-3-3σplasmid for 42 hours.
T10 1404-1458 Sentence denotes Cells in LPS group were stimulated by LPS for 6 hours.
T11 1459-1600 Sentence denotes The protein expressions of Bax and B-cell lymphoma-2 (Bcl-2) were detected by Western blotting, and the ratio of Bax to Bcl-2 was calculated.
T12 1601-1647 Sentence denotes Apoptotic rate was detected by flow cytometry.
T13 1648-1857 Sentence denotes The mRNA expressions of tumor necrosis factor alpha (TNF-α) and interleukin 1beta (IL-1β) in cells were detected by real-time fluorescent quantitative reverse transcription polymerase chain reaction technique.
T14 1858-1963 Sentence denotes Content of TNF-α and IL-1β in cell culture supernatant was detected by enzyme-linked immunosorbent assay.
T15 1964-2078 Sentence denotes Data were statistically analyzed with t test, one-way analysis of variance, and least significant difference test.
T16 2079-2617 Sentence denotes Results: (1) At 48 hours after transfection, the protein expression of 14-3-3σin cells of PCMV6-14-3-3σgroup (1.05±0.03) was significantly higher than that in control group (0.78±0.04, t=5.41, P<0.01). (2) Compared with those in control group, the ratio of Bax to Bcl-2, apoptotic rate, mRNA expressions of TNF-α and IL-1β, and content of TNF-α and IL-1β in cell supernatant in PCMV6-14-3-3σgroup showed no significant difference (P>0.05); the above-mentioned indexes of cells in LPS group were significantly higher or increased (P<0.01).
T17 2618-2764 Sentence denotes Compared with those in LPS group, the above-mentioned indexes of cells in PCMV6-14-3-3σ+ LPS group were significantly lower or decreased (P<0.01).
T18 2765-2777 Sentence denotes Conclusions:
T19 2778-2825 Sentence denotes 14-3-3σis a key factor in regulating apoptosis.
T20 2826-3004 Sentence denotes It can alleviate the LPS-induced inflammatory responses by regulating the ratio of apoptotic regulators Bax to Bcl-2 and inhibiting apoptosis of human pulmonary epithelial cells.