PubMed:27866197
Annnotations
{"target":"http://pubannotation.org/docs/sourcedb/PubMed/sourceid/27866197","sourcedb":"PubMed","sourceid":"27866197","text":"Rs56288038 (C/G) in 3'UTR of IRF-1 Regulated by MiR-502-5p Promotes Gastric Cancer Development.\nBACKGROUND/AIMS: Interferon regulatory factor 1 (IRF-1) has been shown to function as a transcriptional activator or repressor of a variety of target genes. However, its upstream, non-coding RNA-related regulatory capacity remains unknown. In this study, we focus on the miRNA-associated single nucleotide polymorphisms (SNPs) in the 3'untranslated region (UTR) of IRF-1 to further investigate the functional relationship and potential diagnostic value of the SNPs and miRNAs among Chinese gastric cancer (GC) patients.\nMETHODS: We performed a case-control study with 819 GC patients and 756 cancer-free controls. Genotyping by realtime PCR assay, cell transfection, and the dual luciferase reporter assay were used in our study, and the 5-year overall survival rate and relapse-free survival rate in different groups were investigated.\nRESULTS: We found that patients suffering from Helicobacter pylori (Hp) infection were the susceptible population compared to controls. SNP rs56288038 (C/G) in IRF-1 3'UTR was involved in the occurrence of GC by acting as a tumor promoter factor. SNP rs56288038 (C/G) could be up-regulated by miR-502-5p, which caused a down-regulation of IRF-1 in cell lines and decreased apoptosis induced by IFN-γ. Carrying the G genotype was related to significantly low expression of IRF-1 and Hp infection, poor differentiation, big tumor size, invasion depth, as well as the high probability of metastasis, and moreover, the C/G SNP was associated with shorter survival of GC patients with five years of follow-up study.\nCONCLUSIONS: our findings have shown that the SNP rs56288038 (C/G) in IRF-1 3'UTR acted as a promotion factor in GC development through enhancing the regulatory role of miR-502-5p in IRF-1 expression.","tracks":[{"project":"DisGeNET5_variant_disease","denotations":[{"id":"27866197-10#37#47#geners56288038","span":{"begin":1694,"end":1704},"obj":"geners56288038"},{"id":"27866197-10#100#102#diseaseC0024623","span":{"begin":1757,"end":1759},"obj":"diseaseC0024623"},{"id":"27866197-10#100#102#diseaseC0699791","span":{"begin":1757,"end":1759},"obj":"diseaseC0699791"}],"relations":[{"id":"37#47#geners56288038100#102#diseaseC0024623","pred":"associated_with","subj":"27866197-10#37#47#geners56288038","obj":"27866197-10#100#102#diseaseC0024623"},{"id":"37#47#geners56288038100#102#diseaseC0699791","pred":"associated_with","subj":"27866197-10#37#47#geners56288038","obj":"27866197-10#100#102#diseaseC0699791"}],"attributes":[{"subj":"27866197-10#37#47#geners56288038","pred":"source","obj":"DisGeNET5_variant_disease"},{"subj":"27866197-10#100#102#diseaseC0024623","pred":"source","obj":"DisGeNET5_variant_disease"},{"subj":"27866197-10#100#102#diseaseC0699791","pred":"source","obj":"DisGeNET5_variant_disease"}]},{"project":"DisGeNET5_gene_disease","denotations":[{"id":"27866197-0#48#55#gene574504","span":{"begin":48,"end":55},"obj":"gene574504"},{"id":"27866197-0#68#82#diseaseC0024623","span":{"begin":68,"end":82},"obj":"diseaseC0024623"},{"id":"27866197-0#68#82#diseaseC0699791","span":{"begin":68,"end":82},"obj":"diseaseC0699791"},{"id":"27866197-10#57#62#gene3659","span":{"begin":1714,"end":1719},"obj":"gene3659"},{"id":"27866197-10#170#175#gene3659","span":{"begin":1827,"end":1832},"obj":"gene3659"},{"id":"27866197-10#100#102#diseaseC0024623","span":{"begin":1757,"end":1759},"obj":"diseaseC0024623"},{"id":"27866197-10#100#102#diseaseC0699791","span":{"begin":1757,"end":1759},"obj":"diseaseC0699791"},{"id":"27866197-10#100#102#diseaseC0024623","span":{"begin":1757,"end":1759},"obj":"diseaseC0024623"},{"id":"27866197-10#100#102#diseaseC0699791","span":{"begin":1757,"end":1759},"obj":"diseaseC0699791"},{"id":"27866197-9#71#76#gene3659","span":{"begin":1405,"end":1410},"obj":"gene3659"},{"id":"27866197-9#81#83#diseaseC0238339","span":{"begin":1415,"end":1417},"obj":"diseaseC0238339"},{"id":"27866197-9#81#83#diseaseC0333983","span":{"begin":1415,"end":1417},"obj":"diseaseC0333983"}],"relations":[{"id":"48#55#gene57450468#82#diseaseC0024623","pred":"associated_with","subj":"27866197-0#48#55#gene574504","obj":"27866197-0#68#82#diseaseC0024623"},{"id":"48#55#gene57450468#82#diseaseC0699791","pred":"associated_with","subj":"27866197-0#48#55#gene574504","obj":"27866197-0#68#82#diseaseC0699791"},{"id":"57#62#gene3659100#102#diseaseC0024623","pred":"associated_with","subj":"27866197-10#57#62#gene3659","obj":"27866197-10#100#102#diseaseC0024623"},{"id":"57#62#gene3659100#102#diseaseC0699791","pred":"associated_with","subj":"27866197-10#57#62#gene3659","obj":"27866197-10#100#102#diseaseC0699791"},{"id":"57#62#gene3659100#102#diseaseC0024623","pred":"associated_with","subj":"27866197-10#57#62#gene3659","obj":"27866197-10#100#102#diseaseC0024623"},{"id":"57#62#gene3659100#102#diseaseC0699791","pred":"associated_with","subj":"27866197-10#57#62#gene3659","obj":"27866197-10#100#102#diseaseC0699791"},{"id":"170#175#gene3659100#102#diseaseC0024623","pred":"associated_with","subj":"27866197-10#170#175#gene3659","obj":"27866197-10#100#102#diseaseC0024623"},{"id":"170#175#gene3659100#102#diseaseC0699791","pred":"associated_with","subj":"27866197-10#170#175#gene3659","obj":"27866197-10#100#102#diseaseC0699791"},{"id":"170#175#gene3659100#102#diseaseC0024623","pred":"associated_with","subj":"27866197-10#170#175#gene3659","obj":"27866197-10#100#102#diseaseC0024623"},{"id":"170#175#gene3659100#102#diseaseC0699791","pred":"associated_with","subj":"27866197-10#170#175#gene3659","obj":"27866197-10#100#102#diseaseC0699791"},{"id":"71#76#gene365981#83#diseaseC0238339","pred":"associated_with","subj":"27866197-9#71#76#gene3659","obj":"27866197-9#81#83#diseaseC0238339"},{"id":"71#76#gene365981#83#diseaseC0333983","pred":"associated_with","subj":"27866197-9#71#76#gene3659","obj":"27866197-9#81#83#diseaseC0333983"}],"attributes":[{"subj":"27866197-0#48#55#gene574504","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"27866197-0#68#82#diseaseC0024623","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"27866197-0#68#82#diseaseC0699791","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"27866197-10#57#62#gene3659","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"27866197-10#170#175#gene3659","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"27866197-10#100#102#diseaseC0024623","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"27866197-10#100#102#diseaseC0699791","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"27866197-10#100#102#diseaseC0024623","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"27866197-10#100#102#diseaseC0699791","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"27866197-9#71#76#gene3659","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"27866197-9#81#83#dise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