PubMed:24051159 JSONTXT

{"project":"Allie","denotations":[{"id":"SS1_24051159_2_0","span":{"begin":172,"end":196},"obj":"expanded"},{"id":"SS2_24051159_2_0","span":{"begin":198,"end":200},"obj":"abbr"},{"id":"SS1_24051159_6_0","span":{"begin":651,"end":667},"obj":"expanded"},{"id":"SS2_24051159_6_0","span":{"begin":669,"end":671},"obj":"abbr"},{"id":"SS1_24051159_6_1","span":{"begin":686,"end":709},"obj":"expanded"},{"id":"SS2_24051159_6_1","span":{"begin":711,"end":713},"obj":"abbr"}],"relations":[{"id":"AE1_24051159_2_0","pred":"abbreviatedTo","subj":"SS1_24051159_2_0","obj":"SS2_24051159_2_0"},{"id":"AE1_24051159_6_0","pred":"abbreviatedTo","subj":"SS1_24051159_6_0","obj":"SS2_24051159_6_0"},{"id":"AE1_24051159_6_1","pred":"abbreviatedTo","subj":"SS1_24051159_6_1","obj":"SS2_24051159_6_1"}],"text":"DNA-based adaptive immunity protect host from infection-associated periodontal bone resorption via recognition of Porphyromonas gingivalis virulence component.\nBACKGROUND: Porphyromonas gingivalis (Pg) is one of a constellation of oral organisms associated with human chronic periodontitis. While adaptive immunity to periodontal pathogen proteins has been investigated and is an important component of periodontal bone resorption, the effect of periodontal pathogen DNA in eliciting systemic and mucosal antibody and modulating immune responses has not been investigated.\nMETHODS: Rowett rats were locally injected with whole genomic Pg DNA in alum. Escherichia coli (Ec) genomic DNA, Fusobacterium nucleatum (Fn) genomic DNA, and saline/alum injected rats served as controls. After various time points, serum IgG and salivary IgA antibody to Ec, Fn or Pg were detected by ELISA. Serum and salivary antibody reactions with Pg surface antigens were determined by Western blot analyses and the specific antigen was identified by mass spectrometry. Effects of genomic DNA immunization on Pg bacterial colonization and experimental periodontal bone resorption were also evaluated.\nRESULTS: Sera from Pg DNA, Ec DNA and Fn DNA-injected rats did not react with Ec or Fn bacteria. Serum IgG antibody levels to Pg and Pg surface extracts were significantly higher in animals immunized with Pg DNA as compared to the control groups. Rats injected with Pg DNA demonstrated a strong serum IgG and salivary IgA antibody reaction solely to Pg fimbrillin (41kDa), the major protein component of Pg fimbriae. In the Pg DNA-immunized group, the numbers of Pg bacteria in oral cavity and the extent of periodontal bone resorption were significantly reduced after Pg infection.\nCONCLUSIONS: This study suggests that infected hosts may select specific genes from whole genomic DNA of the periodontal pathogen for transcription and presentation. The results indicate that the unique gene selected can initiate a host protective immune response to the parent bacterium."}