PubMed:22579746 JSONTXT

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Derivatization with Bodipy hydrazide allows easy matching of the spots of interest and those obtained by general fluorescent protein staining methods, which facilitates excising target proteins from the gels and identifying them. This method is effective for detecting protein carbonylation in samples of proteins submitted to metal-catalyzed oxidation \"in vitro\" and assessing the effect of hydrogen peroxide and chronological aging on protein oxidative damage in yeast 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