PubMed:17451546 JSONTXT

{"project":"Inflammaging","denotations":[{"id":"T1","span":{"begin":0,"end":89},"obj":"Sentence"},{"id":"T2","span":{"begin":90,"end":115},"obj":"Sentence"},{"id":"T3","span":{"begin":116,"end":298},"obj":"Sentence"},{"id":"T4","span":{"begin":299,"end":320},"obj":"Sentence"},{"id":"T5","span":{"begin":321,"end":430},"obj":"Sentence"},{"id":"T6","span":{"begin":431,"end":471},"obj":"Sentence"},{"id":"T7","span":{"begin":472,"end":567},"obj":"Sentence"},{"id":"T8","span":{"begin":568,"end":728},"obj":"Sentence"},{"id":"T9","span":{"begin":729,"end":737},"obj":"Sentence"},{"id":"T10","span":{"begin":738,"end":923},"obj":"Sentence"},{"id":"T11","span":{"begin":924,"end":990},"obj":"Sentence"},{"id":"T12","span":{"begin":991,"end":1217},"obj":"Sentence"},{"id":"T13","span":{"begin":1218,"end":1338},"obj":"Sentence"},{"id":"T14","span":{"begin":1339,"end":1446},"obj":"Sentence"},{"id":"T15","span":{"begin":1447,"end":1507},"obj":"Sentence"},{"id":"T16","span":{"begin":1508,"end":1519},"obj":"Sentence"},{"id":"T17","span":{"begin":1520,"end":1689},"obj":"Sentence"},{"id":"T18","span":{"begin":1690,"end":1769},"obj":"Sentence"},{"id":"T19","span":{"begin":1770,"end":1860},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":89},"obj":"Sentence"},{"id":"T2","span":{"begin":90,"end":115},"obj":"Sentence"},{"id":"T3","span":{"begin":116,"end":298},"obj":"Sentence"},{"id":"T4","span":{"begin":299,"end":320},"obj":"Sentence"},{"id":"T5","span":{"begin":321,"end":430},"obj":"Sentence"},{"id":"T6","span":{"begin":431,"end":471},"obj":"Sentence"},{"id":"T7","span":{"begin":472,"end":567},"obj":"Sentence"},{"id":"T8","span":{"begin":568,"end":728},"obj":"Sentence"},{"id":"T9","span":{"begin":729,"end":737},"obj":"Sentence"},{"id":"T10","span":{"begin":738,"end":923},"obj":"Sentence"},{"id":"T11","span":{"begin":924,"end":990},"obj":"Sentence"},{"id":"T12","span":{"begin":991,"end":1217},"obj":"Sentence"},{"id":"T13","span":{"begin":1218,"end":1338},"obj":"Sentence"},{"id":"T14","span":{"begin":1339,"end":1446},"obj":"Sentence"},{"id":"T15","span":{"begin":1447,"end":1507},"obj":"Sentence"},{"id":"T16","span":{"begin":1508,"end":1519},"obj":"Sentence"},{"id":"T17","span":{"begin":1520,"end":1689},"obj":"Sentence"},{"id":"T18","span":{"begin":1690,"end":1769},"obj":"Sentence"},{"id":"T19","span":{"begin":1770,"end":1860},"obj":"Sentence"}],"text":"The effect of age on prostaglandin-synthesizing enzymes in the development of gingivitis.\nBACKGROUND AND OBJECTIVE: The aim of this study was to identify the expression of cyclooxygenase-1, cyclooxygenase-2, cyclooxygenase-3, and microsomal prostaglandin E synthase-1 in young and elderly subjects.\nMATERIAL AND METHODS: Periodontally healthy subjects were divided into young (18-30 years, n = 7) and elderly (46-77 years, n = 7). A gingival biopsy was taken at baseline. After experimental gingivitis, clinical examination was repeated and a second biopsy was taken. The expression of cyclooxygenase-1, cyclooxygenase-2, cyclooxygenase-3, and microsomal prostaglandin E synthase-1 was analyzed by means of immunohistochemistry.\nRESULTS: In both healthy age groups, cyclooxygenase-1 and microsomal prostaglandin E synthase-1 were expressed in epithelial cells, endothelial cells and fibroblast-like connective tissue cells. Cyclooxygenase-1 was found in Langerhans' cells of the epithelium. Cyclooxygenase-2 expression was observed in cells exhibiting the morphology of epithelial mitosis cells, and the expression of cyclooxygenase-2 in periodontally healthy elderly subjects was significantly lower (p \u003c or = 0.05). Following experimental gingivitis, cyclooxygenase-1 and microsomal prostaglandin E synthase-1 expression did not change. However, the expression of cyclooxygenase-2 was significantly increased in both age groups (p \u003c or = 0.05). Cyclooxygenase-3 was not detected in any group investigated.\nCONCLUSION: Cyclooxygenase-1 and microsomal prostaglandin E synthase-1 were expressed constitutively in gingival tissue, and expression was unaffected by age or inflammation states. In contrast, the expression of cyclooxygenase-2 was weaker in elderly subjects. In the course of experimental gingivitis, cyclooxygenase-2 was induced in both age groups."}