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PubMed:15194535 JSONTXT

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Inflammaging

Id Subject Object Predicate Lexical cue
T1 0-109 Sentence denotes Real-time polymerase chain reaction determination of cytokine mRNA expression profiles in Hodgkin's lymphoma.
T2 110-136 Sentence denotes BACKGROUND AND OBJECTIVES:
T3 137-265 Sentence denotes Classical Hodgkin's lymphoma (HL) is a malignant disorder characterized by a small number of tumor cells and inflammatory cells.
T4 266-397 Sentence denotes Both the tumor cells and the inflammatory cells produce cytokines which are thought to contribute to the clinical parameters of HL.
T5 398-479 Sentence denotes Quantification of these cytokines at a protein level is still somewhat imprecise.
T6 480-587 Sentence denotes We, therefore, used a method to quantify cytokine mRNA expression in HL cell lines and lymph node biopsies.
T7 588-607 Sentence denotes DESIGN AND METHODS:
T8 608-985 Sentence denotes We used real-time quantitative polymerase chain reaction (RQ-PCR) to investigate mRNA expression for interleukin (IL)-1alpha, IL-1beta, IL-2, IL-4, IL-5, IL-8, IL-12p35, IL-12p40, IL-13, IL-15, interferon (IFN)-gamma and tumor necrosis factor (TNF-alpha) in lymph node tissue from 15 patients with classic Hodgkin's lymphoma (c-HL) and one with lymphocyte predominance (LP) HL.
T9 986-1053 Sentence denotes HL-derived cell lines L1236, L540, and L428 were also investigated.
T10 1054-1209 Sentence denotes Reactive lymphatic tissue (n=6) and peripheral blood mononuclear cells (PBMC) from healthy donors (n=4) before and after stimulation were used as controls.
T11 1210-1304 Sentence denotes In 5 c-HL samples the cytokine expression in T lymphocytes was also studied by flow cytometry.
T12 1305-1313 Sentence denotes RESULTS:
T13 1314-1365 Sentence denotes All c-HL samples (but not LP) expressed IL-13 mRNA.
T14 1366-1449 Sentence denotes This cytokine was not found in non-stimulated PBMC or in reactive lymphatic tissue.
T15 1450-1573 Sentence denotes Expression of IL-10, IL-1beta, IL-15 and IL-12p35 mRNA was higher in HL samples than in PBMC and reactive lymphatic tissue.
T16 1574-1711 Sentence denotes Expression of IL-10, IL-1beta, TNF-alpha and IFN-gamma mRNA was significantly higher in the EBV+ HL samples (n=6) than in the EBV- cases.
T17 1712-1798 Sentence denotes All HL cell lines showed high expression of IL-13, IL-12p35, TNF-alpha and IL-15 mRNA.
T18 1799-1930 Sentence denotes IFNg mRNA levels were high in L428 and L540 cells, IL-10 in L1236 cells and L540 cells, IL-5 in L428 cells and IL-4 in L1236 cells.
T19 1931-1962 Sentence denotes INTERPRETATION AND CONCLUSIONS:
T20 1963-2043 Sentence denotes Cytokine mRNA levels can be measured by RQ-PCR using a limited amount of tissue.
T21 2044-2272 Sentence denotes This method gives valuable information on biological variation between different HL samples and may contribute to unraveling the complex cytokine network contributing to the clinical and biological heterogeneity of this disease.
T1 0-109 Sentence denotes Real-time polymerase chain reaction determination of cytokine mRNA expression profiles in Hodgkin's lymphoma.
T2 110-136 Sentence denotes BACKGROUND AND OBJECTIVES:
T3 137-265 Sentence denotes Classical Hodgkin's lymphoma (HL) is a malignant disorder characterized by a small number of tumor cells and inflammatory cells.
T4 266-397 Sentence denotes Both the tumor cells and the inflammatory cells produce cytokines which are thought to contribute to the clinical parameters of HL.
T5 398-479 Sentence denotes Quantification of these cytokines at a protein level is still somewhat imprecise.
T6 480-587 Sentence denotes We, therefore, used a method to quantify cytokine mRNA expression in HL cell lines and lymph node biopsies.
T7 588-607 Sentence denotes DESIGN AND METHODS:
T8 608-985 Sentence denotes We used real-time quantitative polymerase chain reaction (RQ-PCR) to investigate mRNA expression for interleukin (IL)-1alpha, IL-1beta, IL-2, IL-4, IL-5, IL-8, IL-12p35, IL-12p40, IL-13, IL-15, interferon (IFN)-gamma and tumor necrosis factor (TNF-alpha) in lymph node tissue from 15 patients with classic Hodgkin's lymphoma (c-HL) and one with lymphocyte predominance (LP) HL.
T9 986-1053 Sentence denotes HL-derived cell lines L1236, L540, and L428 were also investigated.
T10 1054-1209 Sentence denotes Reactive lymphatic tissue (n=6) and peripheral blood mononuclear cells (PBMC) from healthy donors (n=4) before and after stimulation were used as controls.
T11 1210-1304 Sentence denotes In 5 c-HL samples the cytokine expression in T lymphocytes was also studied by flow cytometry.
T12 1305-1313 Sentence denotes RESULTS:
T13 1314-1365 Sentence denotes All c-HL samples (but not LP) expressed IL-13 mRNA.
T14 1366-1449 Sentence denotes This cytokine was not found in non-stimulated PBMC or in reactive lymphatic tissue.
T15 1450-1573 Sentence denotes Expression of IL-10, IL-1beta, IL-15 and IL-12p35 mRNA was higher in HL samples than in PBMC and reactive lymphatic tissue.
T16 1574-1711 Sentence denotes Expression of IL-10, IL-1beta, TNF-alpha and IFN-gamma mRNA was significantly higher in the EBV+ HL samples (n=6) than in the EBV- cases.
T17 1712-1798 Sentence denotes All HL cell lines showed high expression of IL-13, IL-12p35, TNF-alpha and IL-15 mRNA.
T18 1799-1930 Sentence denotes IFNg mRNA levels were high in L428 and L540 cells, IL-10 in L1236 cells and L540 cells, IL-5 in L428 cells and IL-4 in L1236 cells.
T19 1931-1962 Sentence denotes INTERPRETATION AND CONCLUSIONS:
T20 1963-2043 Sentence denotes Cytokine mRNA levels can be measured by RQ-PCR using a limited amount of tissue.
T21 2044-2272 Sentence denotes This method gives valuable information on biological variation between different HL samples and may contribute to unraveling the complex cytokine network contributing to the clinical and biological heterogeneity of this disease.

DisGeNET5_gene_disease

Id Subject Object Predicate Lexical cue
15194535-11#31#36#gene3600 1481-1486 gene3600 denotes IL-15
15194535-11#69#71#diseaseC1333064 1519-1521 diseaseC1333064 denotes HL
15194535-12#14#19#gene3586 1588-1593 gene3586 denotes IL-10
15194535-12#31#40#gene7124 1605-1614 gene7124 denotes TNF-alpha
15194535-12#97#99#diseaseC1333064 1671-1673 diseaseC1333064 denotes HL
15194535-13#44#49#gene3596 1756-1761 gene3596 denotes IL-13
15194535-13#4#6#diseaseC1333064 1716-1718 diseaseC1333064 denotes HL
31#36#gene360069#71#diseaseC1333064 15194535-11#31#36#gene3600 15194535-11#69#71#diseaseC1333064 associated_with IL-15,HL
14#19#gene358697#99#diseaseC1333064 15194535-12#14#19#gene3586 15194535-12#97#99#diseaseC1333064 associated_with IL-10,HL
31#40#gene712497#99#diseaseC1333064 15194535-12#31#40#gene7124 15194535-12#97#99#diseaseC1333064 associated_with TNF-alpha,HL
44#49#gene35964#6#diseaseC1333064 15194535-13#44#49#gene3596 15194535-13#4#6#diseaseC1333064 associated_with IL-13,HL

Wangshuguang

Id Subject Object Predicate Lexical cue
T1 275-280 B-Variation denotes tumor
T2 353-363 B-Negative_Regulation denotes contribute
T3 535-545 B-Variation denotes expression
T4 549-551 B-Pathway denotes HL
T5 552-556 I-Pathway denotes cell
T6 557-562 I-Variation denotes lines
T7 656-664 B-Variation denotes reaction
T8 725-732 B-Variation denotes -1alpha
T9 802-812 B-Variation denotes interferon
T10 818-824 B-Variation denotes -gamma
T11 829-834 B-Molecular_Activity denotes tumor
T12 835-843 I-Molecular_Activity denotes necrosis
T13 844-850 I-Molecular_Activity denotes factor
T14 1371-1379 B-Variation denotes cytokine
T15 1500-1504 I-Moleclar_Activity denotes mRNA
T16 1629-1633 I-Mutation denotes mRNA
T17 1716-1718 B-Pathway denotes HL
T18 1719-1723 I-Pathway denotes cell
T19 1724-1729 I-Variation denotes lines
T20 1742-1752 B-Negative_Function denotes expression
T21 1799-1803 I-Pathway denotes IFNg
T22 1804-1808 I-Pathway denotes mRNA
T23 1809-1815 B-Negtive_Regulation denotes levels
T24 2242-2255 B-Variation denotes heterogeneity

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Id Subject Object Predicate Lexical cue
T1 275-280 B-Variation denotes tumor
T2 353-363 B-Negative_Regulation denotes contribute
T3 535-545 B-Variation denotes expression
T4 549-551 B-Pathway denotes HL
T5 552-556 I-Pathway denotes cell
T6 557-562 I-Variation denotes lines
T7 656-664 B-Variation denotes reaction
T8 725-732 B-Variation denotes -1alpha
T9 802-812 B-Variation denotes interferon
T10 818-824 B-Variation denotes -gamma
T11 829-834 B-Molecular_Activity denotes tumor
T12 835-843 I-Molecular_Activity denotes necrosis
T13 844-850 I-Molecular_Activity denotes factor
T14 1371-1379 B-Variation denotes cytokine
T15 1500-1504 I-Moleclar_Activity denotes mRNA
T16 1629-1633 I-Mutation denotes mRNA
T17 1716-1718 B-Pathway denotes HL
T18 1719-1723 I-Pathway denotes cell
T19 1724-1729 I-Variation denotes lines
T20 1742-1752 B-Negative_Function denotes expression
T21 1799-1803 I-Pathway denotes IFNg
T22 1804-1808 I-Pathway denotes mRNA
T23 1809-1815 B-Negtive_Regulation denotes levels
T24 2242-2255 B-Variation denotes heterogeneity