The bacteriophage host Salmonella enterica Serovar Typhimurium LT2 (RD1) bacterium was grown in LB culture medium containing 100 mg/L Ampicillin, added after autoclaving, to select for cells with pRD1 as the PRD1 phage infects cells that harbor a conjugative plasmid [20]. The SM (Sodium-Magnesium) phage dilutant buffer for phage isolation and propagation with a pH of 7.4 was prepared by adding 5.8 g of NaCl, 2.0 g of MgSO4·7 H2O, and 50 mL of 1 M Tris-HCl per 1 L of MQ water, autoclaved, 0.2 μm filter sterilized and stored at room temperature. Calcium chloride (CaCl2) solution was prepared as a filter-sterilized 1 M stock solution and added to the LB broth to a final concentration of 0.001 M. The overlay was prepared by adding 0.1 mL of fresh mid-log phase (OD600 = 1.0) S. enterica host culture incubated at 37 °C, and 0.1 mL of phage (102–103 plaque forming units (PFU)/mL) to 4 mL of soft agar containing 100 mg/L Ampicillin at 56 °C in a 5 mL tube, poured over an LB agar plate and incubated overnight at 37 °C.