Second, based on results obtained from the test scans (N = 6), agreement between regional quantification (for all RRs and reference tissue methods) and the gold standard (DVRPI_GMCB) was assessed using Bland–Altman (BA) analysis [27]. Next, linear regression analysis of the data points in the BA plots was used to assess whether (and to what extent) bias was dependent on underlying amyloid burden. Finally, correlations, slopes and intercepts between DVRPI_GMCB and the corresponding parameter of interest derived from each of the RRs and methods were calculated using linear regression analysis.